Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004140

Physical Location & Seq

Genomic Coordinates Reverse (-)

18656955 .. 18658377

Gene Structure 1423 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G50330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 696 bp

ATGGATAACTCCGACATTCTAATGAACATGATGATGCAGCAGATGGAGAAGCTTCCTGAACACTTCTCTAACTCAAACCCTAACCCTAATCCCCATAACATTATGATGCTTTCTGAATCCAACACCCACCCGTTCTTCTTCAACCCCACTCATTCTCATCTCCCATTTGACCAAACCATGCCTCACCACCAACCCGGTTTAAATTTCCGGTACGCCCCCTCCCCGTCATCATCTCTCCCGGAGAAGAGAGGAGGCTGCAGCGACAACGCCAACATGGCGGCGATGAGAGAGATGATCTTTCGAATAGCCGTGATGCAGCCTATACATATTGATCCGGAATCCGTAAAGCCACCAAAGAGAAAGAACGTGAGGATCTCTAAGGATCCACAGAGCGTGGCAGCTCGGCATCGAAGGGAGAGGATAAGCGAGCGGATTCGGATTCTTCAGCGGCTTGTTCCCGGTGGGACTAAGATGGATACGGCGTCGATGCTCGATGAGGCTATCCATTACGTTAAGTTTCTCAAGAAGCAAGTGCAGTCGCTGGAGGAACATGCGGTGGTTAACGGCGGAGGAATGACGGCGGTGGCCGGAGGAGCACTTGCGGGTACTGTTGGTGGAGGATATGGAGGAAAAGGGTGTGGCATTATGCGGTCTGATCATCACCAGATGCTTGGAAATGCACAGATTCTTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0010468 GO:0010500 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0035670 GO:0043565 GO:0045893 GO:0045935 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048444 GO:0048445 GO:0048449 GO:0048462 GO:0048467 GO:0048518 GO:0048522 GO:0048608 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0080126 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1905328 GO:1905392 GO:1905393 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

231

Amino Acids

25.68

Weight (kDa)

9.51

Isoelectric Point (pI)

55.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	134 - 175	3.1e-07	Helix-loop-helix DNA-binding domain
bHLH_SAC51	PF23173	136 - 180	1.6e-12	Transcription factor SAC51 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50330 AT5G67060 AT5G67060
fragaria_vesca	FvH4_1g16130 FvH4_2g34280
malus_domestica	MD02G1173400.v1.1 MD08G1098800.v1.1 MD15G1081100.v1.1 MD15G1284900.v1.1
prunus_persica	Prupe.1G434000_v2.0.a1 Prupe.7G132300_v2.0.a1
pyrus_communis	pycom15g07560 pycom15g07580
rosa_chinensis	RchiOBHm_Chr2g0105811 RchiOBHm_Chr6g0308101
rosa_laevigata	RLG00000010668 RLG00000017461
rosa_multiflora	Rmu_sc0005706.1_g000001 Rmu_sc0010497.1_g000002
rosa_roxburghii	Rroxscaffold_2G00137880 Rroxscaffold_7G00160540
rosa_rugosa	Rorug02G0130500 Rorug06G0364000
rosa_samantha	Rh2AG181700 Rh2BG191300 Rh2CG186000 Rh2DG187900 Rh6AG472700 Rh6BG483900 Rh6CG488800 Rh6DG475100
rosa_wichuraiana	Rw2G014230 Rw6G041250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	430
AccIII	TCCGGA	1 cut(s)	334
AciI	CCGC	8 cut(s)	278, 430, 448, 554, 567, 581, 602, 649
AclWI	GGATC	4 cut(s)	326, 377, 380, 390
AcoI	YGGCCR	1 cut(s)	585
AcsI	RAATTY	1 cut(s)	202
AcuI	CTGAAG	1 cut(s)	428
AcyI	GRCGYC	1 cut(s)	482
AfaI	GTAC	2 cut(s)	212, 607
AgsI	TTSAA	1 cut(s)	142
AluBI	AGCT	2 cut(s)	52, 401
AluI	AGCT	2 cut(s)	52, 401
Alw21I	GWGCWC	1 cut(s)	598
AlwI	GGATC	4 cut(s)	326, 377, 380, 390
AlwNI	CAGNNNCTG	1 cut(s)	541
Aor13HI	TCCGGA	1 cut(s)	334
AoxI	GGCC	1 cut(s)	585
ApeKI	GCWGC	5 cut(s)	37, 255, 258, 316, 398
ApoI	RAATTY	1 cut(s)	202
AsuC2I	CCSGG	3 cut(s)	195, 239, 459
AsuHPI	GGTGA	2 cut(s)	176, 653
AsuII	TTCGAA	1 cut(s)	301
BamHI	GGATCC	1 cut(s)	382
Bbv12I	GWGCWC	1 cut(s)	598
BbvI	GCAGC	5 cut(s)	49, 242, 270, 328, 410
BccI	CCATC	2 cut(s)	37, 466
BceAI	ACGGC	4 cut(s)	293, 495, 580, 594
BciVI	GTATCC	1 cut(s)	469
BclI	TGATCA	1 cut(s)	655
BcnI	CCSGG	3 cut(s)	195, 239, 459
BfmI	CTRYAG	1 cut(s)	256
BfuI	GTATCC	1 cut(s)	469
BglI	GCCNNNNNGGC	1 cut(s)	275
BisI	GCNGC	7 cut(s)	38, 256, 259, 279, 317, 399, 449
BlsI	GCNGC	7 cut(s)	39, 257, 260, 280, 318, 400, 450
Bme1390I	CCNGG	3 cut(s)	195, 239, 459
BmiI	GGNNCC	1 cut(s)	384
BmrFI	CCNGG	3 cut(s)	195, 239, 459
BmsI	GCATC	6 cut(s)	24, 96, 303, 415, 477, 657
BpmI	CTGGAG	1 cut(s)	563
Bpu14I	TTCGAA	1 cut(s)	301
BpuEI	CTTGAG	1 cut(s)	506
BpuMI	CCSGG	3 cut(s)	195, 239, 459
BsaHI	GRCGYC	1 cut(s)	482
BsaWI	WCCGGW	2 cut(s)	207, 334
BsaXI	ACNNNNNCTCC	2 cut(s)	203, 233
BseAI	TCCGGA	1 cut(s)	334
BseRI	GAGGAG	2 cut(s)	264, 606
BseXI	GCAGC	5 cut(s)	49, 242, 270, 328, 410
BsgI	GTGCAG	1 cut(s)	554
BshFI	GGCC	1 cut(s)	587
BsiHKAI	GWGCWC	1 cut(s)	598
BsiSI	CCGG	6 cut(s)	195, 208, 239, 335, 459, 588
BslFI	GGGAC	1 cut(s)	478
BsmFI	GGGAC	1 cut(s)	478
BsnI	GGCC	1 cut(s)	587
Bsp119I	TTCGAA	1 cut(s)	301
Bsp1286I	GDGCHC	1 cut(s)	598
Bsp13I	TCCGGA	1 cut(s)	334
Bsp143I	GATC	5 cut(s)	294, 331, 372, 382, 655
BspACI	CCGC	8 cut(s)	278, 430, 448, 554, 567, 581, 602, 649
BspANI	GGCC	1 cut(s)	587
BspEI	TCCGGA	1 cut(s)	334
BspLI	GGNNCC	1 cut(s)	384
BspMAI	CTGCAG	1 cut(s)	260
BspPI	GGATC	4 cut(s)	326, 377, 380, 390
BspT104I	TTCGAA	1 cut(s)	301
BsrBI	CCGCTC	1 cut(s)	430
BssMI	GATC	5 cut(s)	294, 331, 372, 382, 655
BssNI	GRCGYC	1 cut(s)	482
Bst4CI	ACNGT	1 cut(s)	610
Bst6I	CTCTTC	1 cut(s)	239
BstACI	GRCGYC	1 cut(s)	482
BstBI	TTCGAA	1 cut(s)	301
BstC8I	GCNNGC	1 cut(s)	428
BstDEI	CTNAG	3 cut(s)	378, 468, 689
BstKTI	GATC	5 cut(s)	297, 334, 375, 385, 658
BstMBI	GATC	5 cut(s)	294, 331, 372, 382, 655
BstMWI	GCNNNNNNNGC	2 cut(s)	275, 593
BstNSI	RCATGY	1 cut(s)	554
BstSCI	CCNGG	3 cut(s)	193, 237, 457
BstSFI	CTRYAG	1 cut(s)	256
BstV1I	GCAGC	5 cut(s)	49, 242, 270, 328, 410
BstX2I	RGATCY	2 cut(s)	372, 382
BstXI	CCANNNNNNTGG	1 cut(s)	671
BstYI	RGATCY	2 cut(s)	372, 382
BsuI	GTATCC	1 cut(s)	469
BsuRI	GGCC	1 cut(s)	587
BtgZI	GCGATG	1 cut(s)	296
Cac8I	GCNNGC	1 cut(s)	428
CaiI	CAGNNNCTG	1 cut(s)	541
CseI	GACGC	1 cut(s)	471
Csp6I	GTAC	2 cut(s)	211, 606
CviAII	CATG	4 cut(s)	28, 178, 274, 551
CviJI	RGCY	9 cut(s)	52, 255, 308, 319, 349, 401, 451, 500, 587
CviKI_1	RGCY	9 cut(s)	52, 255, 308, 319, 349, 401, 451, 500, 587
CviQI	GTAC	2 cut(s)	211, 606
DdeI	CTNAG	3 cut(s)	378, 468, 689
DpnI	GATC	5 cut(s)	296, 333, 374, 384, 657
DpnII	GATC	5 cut(s)	294, 331, 372, 382, 655
DraI	TTTAAA	1 cut(s)	201
EaeI	YGGCCR	1 cut(s)	585
Eam1104I	CTCTTC	1 cut(s)	239
EarI	CTCTTC	1 cut(s)	239
EciI	GGCGGA	1 cut(s)	582
Eco57I	CTGAAG	1 cut(s)	428
FaeI	CATG	4 cut(s)	31, 181, 277, 554
FaqI	GGGAC	1 cut(s)	478
FatI	CATG	4 cut(s)	27, 177, 273, 550
FauI	CCCGC	1 cut(s)	595
FbaI	TGATCA	1 cut(s)	655
Fnu4HI	GCNGC	7 cut(s)	38, 256, 259, 279, 317, 399, 449
Fsp4HI	GCNGC	7 cut(s)	38, 256, 259, 279, 317, 399, 449
GluI	GCNGC	7 cut(s)	38, 256, 259, 279, 317, 399, 449
GsuI	CTGGAG	1 cut(s)	563
HaeIII	GGCC	1 cut(s)	587
HapII	CCGG	6 cut(s)	195, 208, 239, 335, 459, 588
HgaI	GACGC	1 cut(s)	471
Hin1I	GRCGYC	1 cut(s)	482
Hin1II	CATG	4 cut(s)	31, 181, 277, 554
HincII	GTYRAC	1 cut(s)	562
HindII	GTYRAC	1 cut(s)	562
HindIII	AAGCTT	1 cut(s)	50
HinfI	GANTC	5 cut(s)	116, 338, 433, 439, 685
HpaI	GTTAAC	1 cut(s)	562
HpaII	CCGG	6 cut(s)	195, 208, 239, 335, 459, 588
HphI	GGTGA	2 cut(s)	176, 653
Hpy166II	GTNNAC	1 cut(s)	562
Hpy188I	TCNGA	4 cut(s)	13, 115, 438, 655
Hpy188III	TCNNGA	3 cut(s)	56, 335, 523
Hpy8I	GTNNAC	1 cut(s)	562
Hpy99I	CGWCG	1 cut(s)	487
HpyAV	CCTTC	1 cut(s)	405
HpyCH4III	ACNGT	1 cut(s)	610
HpyCH4IV	ACGT	2 cut(s)	366, 510
HpyCH4V	TGCA	5 cut(s)	37, 258, 316, 535, 680
HpyF10VI	GCNNNNNNNGC	2 cut(s)	275, 593
HpyF3I	CTNAG	3 cut(s)	378, 468, 689
HpySE526I	ACGT	2 cut(s)	366, 510
Hsp92I	GRCGYC	1 cut(s)	482
Hsp92II	CATG	4 cut(s)	31, 181, 277, 554
Kpn2I	TCCGGA	1 cut(s)	334
Ksp22I	TGATCA	1 cut(s)	655
KspAI	GTTAAC	1 cut(s)	562
Kzo9I	GATC	5 cut(s)	294, 331, 372, 382, 655
LmnI	GCTCC	1 cut(s)	593
LpnPI	CCDG	9 cut(s)	69, 208, 221, 252, 348, 472, 527, 601, 677
Lsp1109I	GCAGC	5 cut(s)	49, 242, 270, 328, 410
LweI	GCATC	6 cut(s)	24, 96, 303, 415, 477, 657
MaeII	ACGT	2 cut(s)	366, 510
MalI	GATC	5 cut(s)	296, 333, 374, 384, 657
MbiI	CCGCTC	1 cut(s)	430
MboI	GATC	5 cut(s)	294, 331, 372, 382, 655
MboII	GAAGA	4 cut(s)	127, 130, 256, 434
MflI	RGATCY	2 cut(s)	372, 382
MhlI	GDGCHC	1 cut(s)	598
MluCI	AATT	1 cut(s)	202
MmeI	TCCRAC	2 cut(s)	36, 144
MroI	TCCGGA	1 cut(s)	334
MseI	TTAA	3 cut(s)	200, 513, 561
MslI	CAYNNNNRTG	3 cut(s)	20, 32, 104
MspA1I	CMGCKG	1 cut(s)	448
MspI	CCGG	6 cut(s)	195, 208, 239, 335, 459, 588
MspR9I	CCNGG	3 cut(s)	195, 239, 459
MwoI	GCNNNNNNNGC	2 cut(s)	275, 593
NciI	CCSGG	3 cut(s)	195, 239, 459
NdeII	GATC	5 cut(s)	294, 331, 372, 382, 655
NlaIII	CATG	4 cut(s)	31, 181, 277, 554
NlaIV	GGNNCC	1 cut(s)	384
NmeAIII	GCCGAG	1 cut(s)	382
NspI	RCATGY	1 cut(s)	554
NspV	TTCGAA	1 cut(s)	301
PfeI	GAWTC	5 cut(s)	116, 338, 433, 439, 685
PfoI	TCCNGGA	1 cut(s)	237
PkrI	GCNGC	7 cut(s)	39, 257, 260, 280, 318, 400, 450
PspN4I	GGNNCC	1 cut(s)	384
PstI	CTGCAG	1 cut(s)	260
PstNI	CAGNNNCTG	1 cut(s)	541
PsuI	RGATCY	2 cut(s)	372, 382
RsaI	GTAC	2 cut(s)	212, 607
RsaNI	GTAC	2 cut(s)	211, 606
RseI	CAYNNNNRTG	3 cut(s)	20, 32, 104
SaqAI	TTAA	3 cut(s)	200, 513, 561
SatI	GCNGC	7 cut(s)	38, 256, 259, 279, 317, 399, 449
Sau3AI	GATC	5 cut(s)	294, 331, 372, 382, 655
ScrFI	CCNGG	3 cut(s)	195, 239, 459
SduI	GDGCHC	1 cut(s)	598
SetI	ASST	4 cut(s)	54, 369, 403, 513
SfaNI	GCATC	6 cut(s)	24, 96, 303, 415, 477, 657
SfcI	CTRYAG	1 cut(s)	256
SfuI	TTCGAA	1 cut(s)	301
SmiMI	CAYNNNNRTG	3 cut(s)	20, 32, 104
SmlI	CTYRAG	1 cut(s)	521
SmoI	CTYRAG	1 cut(s)	521
Sse9I	AATT	1 cut(s)	202
SsiI	CCGC	8 cut(s)	278, 430, 448, 554, 567, 581, 602, 649
StyD4I	CCNGG	3 cut(s)	193, 237, 457
TaaI	ACNGT	1 cut(s)	610
TaiI	ACGT	2 cut(s)	369, 513
TaqI	TCGA	4 cut(s)	301, 409, 485, 492
TasI	AATT	1 cut(s)	202
TauI	GCSGC	2 cut(s)	281, 451
TfiI	GAWTC	5 cut(s)	116, 338, 433, 439, 685
Tru1I	TTAA	3 cut(s)	200, 513, 561
Tru9I	TTAA	3 cut(s)	200, 513, 561
TseI	GCWGC	5 cut(s)	37, 255, 258, 316, 398
TspDTI	ATGAA	1 cut(s)	38
TspGWI	ACGGA	1 cut(s)	331
XapI	RAATTY	1 cut(s)	202
XceI	RCATGY	1 cut(s)	554

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.