Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004140

Physical Location & Seq

Genomic Coordinates Forward (+)

5551948 .. 5552700

Gene Structure 753 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1081100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 753 bp

ATGGATATTGGCCAGTACAAATCAGAGGAGCAGATGGAGATGATGATGATGATGCAAATGGAAAAAATATCCGAGCTCTGCGGTGCCTACAACGACGTTGTTTCCGACCTGCCGCAGTCCGATCTCAATTTCTCCTCCACCACAACTAGTCCCAATAGTATCAGTGCCACCATGTCTCATTACACTACAGACCAAAACCCACATAACAATAGTATCCATTCACCAAACTCAAACTCACAATCCTTCCTAAACCTTCCAACAATCCCATCTACCATTTCATTCACCAACACAAACCCTACCGAGCAAACATTGTTTTCAACACCGTCACAAAAACGTGCTTCGATGGCAGCCATGAGGGAGATGATATTCCGGATTGCAGCCATGCAGCCAATCAACATCGACCCGGAATCGGTTAAGCCGCCAAAGAGAAGGAACGTGAAGATTTCTAAGGACCCTCAGAGCGTGGCGGCCCGGCATCGGAGAGAGAGGATTAGTGAGAGGATTAGGATCCTGCAGAGACTTGTCCCGGGCGGGACAAAAATGGACACCGCTTCCATGCTAGATGAGGCCATTCACTATGTCAAGTTTTTGAAGACTCAGGTCCAAACGCTGGAGAGAGCTGCGACTAATAGGCCTACCGGGATTGGATTTCCTGTGGCCATGTCTAGTGGGAGCTACATATCTAACTCTATGGCTAAGGCTTATCAGGCTCCTCCTTCTCATCAGAATGTGCAGCATTTCGGTGATGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

251

Amino Acids

27.9

Weight (kDa)

8.99

Isoelectric Point (pI)

55.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	157 - 198	3.5e-07	Helix-loop-helix DNA-binding domain
bHLH_SAC51	PF23173	159 - 203	9.2e-12	Transcription factor SAC51 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50330 AT5G67060 AT5G67060
fragaria_vesca	FvH4_1g16130 FvH4_2g34280
malus_domestica	MD02G1173400.v1.1 MD08G1098800.v1.1 MD15G1081100.v1.1 MD15G1284900.v1.1
prunus_persica	Prupe.1G434000_v2.0.a1 Prupe.7G132300_v2.0.a1
pyrus_communis	pycom15g07560 pycom15g07580
rosa_chinensis	RchiOBHm_Chr2g0105811 RchiOBHm_Chr6g0308101
rosa_laevigata	RLG00000010668 RLG00000017461
rosa_multiflora	Rmu_sc0005706.1_g000001 Rmu_sc0010497.1_g000002
rosa_roxburghii	Rroxscaffold_2G00137880 Rroxscaffold_7G00160540
rosa_rugosa	Rorug02G0130500 Rorug06G0364000
rosa_samantha	Rh2AG181700 Rh2BG191300 Rh2CG186000 Rh2DG187900 Rh6AG472700 Rh6BG483900 Rh6CG488800 Rh6DG475100
rosa_wichuraiana	Rw2G014230 Rw6G041250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	117
AccB1I	GGYRCC	1 cut(s)	83
AccB7I	CCANNNNNTGG	1 cut(s)	610
AccIII	TCCGGA	1 cut(s)	369
AciI	CCGC	6 cut(s)	81, 113, 419, 467, 531, 549
AclWI	GGATC	2 cut(s)	502, 515
AcoI	YGGCCR	2 cut(s)	10, 657
AfaI	GTAC	1 cut(s)	17
AfiI	CCNNNNNNNGG	3 cut(s)	409, 477, 610
AgsI	TTSAA	2 cut(s)	318, 592
AhlI	ACTAGT	1 cut(s)	146
AluBI	AGCT	3 cut(s)	76, 620, 675
AluI	AGCT	3 cut(s)	76, 620, 675
Alw21I	GWGCWC	1 cut(s)	78
Alw26I	GTCTC	2 cut(s)	180, 511
AlwI	GGATC	2 cut(s)	502, 515
Ama87I	CYCGRG	1 cut(s)	526
Aor13HI	TCCGGA	1 cut(s)	369
AoxI	GGCC	5 cut(s)	10, 468, 567, 632, 657
ApeKI	GCWGC	5 cut(s)	347, 377, 385, 620, 733
AspS9I	GGNCC	3 cut(s)	451, 469, 601
AsuC2I	CCSGG	5 cut(s)	404, 472, 527, 528, 640
AsuHPI	GGTGA	2 cut(s)	213, 274
AvaI	CYCGRG	1 cut(s)	526
AvaII	GGWCC	2 cut(s)	451, 601
BaeI	ACNNNNGTAYC	2 cut(s)	196, 229
BalI	TGGCCA	2 cut(s)	12, 659
BamHI	GGATCC	1 cut(s)	507
BanI	GGYRCC	1 cut(s)	83
BanII	GRGCYC	1 cut(s)	78
BbsI	GAAGAC	1 cut(s)	599
Bbv12I	GWGCWC	1 cut(s)	78
BbvI	GCAGC	5 cut(s)	359, 389, 397, 607, 745
BccI	CCATC	3 cut(s)	28, 274, 337
BciVI	GTATCC	1 cut(s)	224
BcnI	CCSGG	5 cut(s)	404, 472, 527, 528, 640
BcoDI	GTCTC	2 cut(s)	180, 511
BcuI	ACTAGT	1 cut(s)	146
BfaI	CTAG	3 cut(s)	147, 560, 666
BfmI	CTRYAG	2 cut(s)	186, 512
BfuAI	ACCTGC	1 cut(s)	117
BfuI	GTATCC	1 cut(s)	224
BisI	GCNGC	8 cut(s)	113, 348, 378, 386, 419, 468, 621, 734
BlsI	GCNGC	8 cut(s)	114, 349, 379, 387, 420, 469, 622, 735
Bme1390I	CCNGG	5 cut(s)	404, 472, 527, 528, 640
Bme18I	GGWCC	2 cut(s)	451, 601
BmeT110I	CYCGRG	1 cut(s)	526
BmgT120I	GGNCC	3 cut(s)	451, 469, 601
BmiI	GGNNCC	4 cut(s)	85, 453, 509, 711
BmrFI	CCNGG	5 cut(s)	404, 472, 527, 528, 640
BmsI	GCATC	3 cut(s)	42, 484, 736
BoxI	GACNNNNGTC	1 cut(s)	599
BpiI	GAAGAC	1 cut(s)	599
BpmI	CTGGAG	1 cut(s)	632
Bpu10I	CCTNAGC	1 cut(s)	696
BpuMI	CCSGG	5 cut(s)	404, 472, 527, 528, 640
BsaBI	GATNNNNATC	1 cut(s)	506
BsaJI	CCNNGG	1 cut(s)	526
BsaWI	WCCGGW	1 cut(s)	369
Bsc4I	CCNNNNNNNGG	3 cut(s)	409, 477, 610
Bse1I	ACTGG	1 cut(s)	13
Bse8I	GATNNNNATC	1 cut(s)	506
BseAI	TCCGGA	1 cut(s)	369
BseDI	CCNNGG	1 cut(s)	526
BseJI	GATNNNNATC	1 cut(s)	506
BseLI	CCNNNNNNNGG	3 cut(s)	409, 477, 610
BseMII	CTCAG	2 cut(s)	470, 611
BseNI	ACTGG	1 cut(s)	13
BseRI	GAGGAG	3 cut(s)	41, 124, 702
BseXI	GCAGC	5 cut(s)	359, 389, 397, 607, 745
BsgI	GTGCAG	1 cut(s)	752
BshFI	GGCC	5 cut(s)	12, 470, 569, 634, 659
BshNI	GGYRCC	1 cut(s)	83
BsiHKAI	GWGCWC	1 cut(s)	78
BsiHKCI	CYCGRG	1 cut(s)	526
BsiSI	CCGG	5 cut(s)	370, 404, 472, 527, 639
BslFI	GGGAC	3 cut(s)	135, 509, 547
BslI	CCNNNNNNNGG	3 cut(s)	409, 477, 610
BsmAI	GTCTC	2 cut(s)	180, 511
BsmFI	GGGAC	3 cut(s)	135, 509, 547
BsnI	GGCC	5 cut(s)	12, 470, 569, 634, 659
BsoBI	CYCGRG	1 cut(s)	526
Bsp1286I	GDGCHC	1 cut(s)	78
Bsp13I	TCCGGA	1 cut(s)	369
Bsp143I	GATC	2 cut(s)	121, 507
BspACI	CCGC	6 cut(s)	81, 113, 419, 467, 531, 549
BspANI	GGCC	5 cut(s)	12, 470, 569, 634, 659
BspCNI	CTCAG	2 cut(s)	469, 610
BspEI	TCCGGA	1 cut(s)	369
BspLI	GGNNCC	4 cut(s)	85, 453, 509, 711
BspMAI	CTGCAG	1 cut(s)	516
BspMI	ACCTGC	1 cut(s)	117
BspPI	GGATC	2 cut(s)	502, 515
BspT107I	GGYRCC	1 cut(s)	83
BsrI	ACTGG	1 cut(s)	13
BssECI	CCNNGG	1 cut(s)	526
BssMI	GATC	2 cut(s)	121, 507
Bst4CI	ACNGT	1 cut(s)	324
BstDEI	CTNAG	4 cut(s)	447, 456, 597, 696
BstKTI	GATC	2 cut(s)	124, 510
BstMAI	GTCTC	2 cut(s)	180, 511
BstMBI	GATC	2 cut(s)	121, 507
BstMWI	GCNNNNNNNGC	2 cut(s)	344, 707
BstPAI	GACNNNNGTC	1 cut(s)	599
BstSCI	CCNGG	5 cut(s)	402, 470, 525, 526, 638
BstSFI	CTRYAG	2 cut(s)	186, 512
BstV1I	GCAGC	5 cut(s)	359, 389, 397, 607, 745
BstV2I	GAAGAC	1 cut(s)	599
BstX2I	RGATCY	1 cut(s)	507
BstYI	RGATCY	1 cut(s)	507
BsuI	GTATCC	1 cut(s)	224
BsuRI	GGCC	5 cut(s)	12, 470, 569, 634, 659
BtsIMutI	CAGTG	1 cut(s)	169
BveI	ACCTGC	1 cut(s)	117
Cfr13I	GGNCC	3 cut(s)	451, 469, 601
Cfr9I	CCCGGG	1 cut(s)	526
Csp6I	GTAC	1 cut(s)	16
CviAII	CATG	5 cut(s)	172, 352, 382, 556, 661
CviQI	GTAC	1 cut(s)	16
DdeI	CTNAG	4 cut(s)	447, 456, 597, 696
DpnI	GATC	2 cut(s)	123, 509
DpnII	GATC	2 cut(s)	121, 507
EaeI	YGGCCR	2 cut(s)	10, 657
Ecl136II	GAGCTC	1 cut(s)	76
Eco147I	AGGCCT	1 cut(s)	634
Eco24I	GRGCYC	1 cut(s)	78
Eco47I	GGWCC	2 cut(s)	451, 601
Eco53kI	GAGCTC	1 cut(s)	76
Eco88I	CYCGRG	1 cut(s)	526
EcoICRI	GAGCTC	1 cut(s)	76
EcoO109I	RGGNCCY	1 cut(s)	451
EcoT38I	GRGCYC	1 cut(s)	78
FaeI	CATG	5 cut(s)	175, 355, 385, 559, 664
FaiI	YATR	9 cut(s)	173, 204, 353, 383, 557, 579, 662, 680, 692
FaqI	GGGAC	3 cut(s)	135, 509, 547
FatI	CATG	5 cut(s)	171, 351, 381, 555, 660
FauI	CCCGC	1 cut(s)	524
Fnu4HI	GCNGC	8 cut(s)	113, 348, 378, 386, 419, 468, 621, 734
FriOI	GRGCYC	1 cut(s)	78
Fsp4HI	GCNGC	8 cut(s)	113, 348, 378, 386, 419, 468, 621, 734
FspBI	CTAG	3 cut(s)	147, 560, 666
GluI	GCNGC	8 cut(s)	113, 348, 378, 386, 419, 468, 621, 734
GsuI	CTGGAG	1 cut(s)	632
HaeIII	GGCC	5 cut(s)	12, 470, 569, 634, 659
HapII	CCGG	5 cut(s)	370, 404, 472, 527, 639
Hin1II	CATG	5 cut(s)	175, 355, 385, 559, 664
HinfI	GANTC	2 cut(s)	407, 595
HpaII	CCGG	5 cut(s)	370, 404, 472, 527, 639
HphI	GGTGA	2 cut(s)	213, 274
Hpy188I	TCNGA	7 cut(s)	25, 73, 106, 121, 459, 480, 726
Hpy188III	TCNNGA	1 cut(s)	370
Hpy99I	CGWCG	1 cut(s)	98
HpyAV	CCTTC	4 cut(s)	253, 263, 423, 726
HpyCH4III	ACNGT	1 cut(s)	324
HpyCH4IV	ACGT	3 cut(s)	96, 334, 435
HpyCH4V	TGCA	5 cut(s)	55, 377, 385, 514, 733
HpyF10VI	GCNNNNNNNGC	2 cut(s)	344, 707
HpyF3I	CTNAG	4 cut(s)	447, 456, 597, 696
HpySE526I	ACGT	3 cut(s)	96, 334, 435
Hsp92II	CATG	5 cut(s)	175, 355, 385, 559, 664
Kpn2I	TCCGGA	1 cut(s)	369
Kzo9I	GATC	2 cut(s)	121, 507
LmnI	GCTCC	3 cut(s)	28, 672, 715
Lsp1109I	GCAGC	5 cut(s)	359, 389, 397, 607, 745
LweI	GCATC	3 cut(s)	42, 484, 736
MaeI	CTAG	3 cut(s)	147, 560, 666
MaeII	ACGT	3 cut(s)	96, 334, 435
MaeIII	GTNAC	1 cut(s)	324
MalI	GATC	2 cut(s)	123, 509
MboI	GATC	2 cut(s)	121, 507
MboII	GAAGA	2 cut(s)	451, 604
MflI	RGATCY	1 cut(s)	507
MhlI	GDGCHC	1 cut(s)	78
MlsI	TGGCCA	2 cut(s)	12, 659
MluCI	AATT	1 cut(s)	127
MluNI	TGGCCA	2 cut(s)	12, 659
MlyI	GAGTC	1 cut(s)	589
MmeI	TCCRAC	2 cut(s)	129, 281
MnlI	CCTC	8 cut(s)	19, 145, 348, 465, 480, 492, 559, 723
Mox20I	TGGCCA	2 cut(s)	12, 659
MroI	TCCGGA	1 cut(s)	369
MscI	TGGCCA	2 cut(s)	12, 659
MseI	TTAA	2 cut(s)	414, 751
MslI	CAYNNNNRTG	2 cut(s)	726, 741
Msp20I	TGGCCA	2 cut(s)	12, 659
MspI	CCGG	5 cut(s)	370, 404, 472, 527, 639
MspR9I	CCNGG	5 cut(s)	404, 472, 527, 528, 640
MwoI	GCNNNNNNNGC	2 cut(s)	344, 707
NciI	CCSGG	5 cut(s)	404, 472, 527, 528, 640
NdeII	GATC	2 cut(s)	121, 507
NlaIII	CATG	5 cut(s)	175, 355, 385, 559, 664
NlaIV	GGNNCC	4 cut(s)	85, 453, 509, 711
NmuCI	GTSAC	1 cut(s)	324
PceI	AGGCCT	1 cut(s)	634
PcsI	WCGNNNNNNNCGW	1 cut(s)	102
PfeI	GAWTC	1 cut(s)	407
PflMI	CCANNNNNTGG	1 cut(s)	610
PkrI	GCNGC	8 cut(s)	114, 349, 379, 387, 420, 469, 622, 735
PleI	GAGTC	1 cut(s)	589
PpsI	GAGTC	1 cut(s)	589
PpuMI	RGGWCCY	1 cut(s)	451
PshAI	GACNNNNGTC	1 cut(s)	599
Psp124BI	GAGCTC	1 cut(s)	78
Psp5II	RGGWCCY	1 cut(s)	451
PspN4I	GGNNCC	4 cut(s)	85, 453, 509, 711
PspPI	GGNCC	3 cut(s)	451, 469, 601
PspPPI	RGGWCCY	1 cut(s)	451
PstI	CTGCAG	1 cut(s)	516
PsuI	RGATCY	1 cut(s)	507
RsaI	GTAC	1 cut(s)	17
RsaNI	GTAC	1 cut(s)	16
RseI	CAYNNNNRTG	2 cut(s)	726, 741
SacI	GAGCTC	1 cut(s)	78
SaqAI	TTAA	2 cut(s)	414, 751
SatI	GCNGC	8 cut(s)	113, 348, 378, 386, 419, 468, 621, 734
Sau3AI	GATC	2 cut(s)	121, 507
Sau96I	GGNCC	3 cut(s)	451, 469, 601
SchI	GAGTC	1 cut(s)	589
ScrFI	CCNGG	5 cut(s)	404, 472, 527, 528, 640
SduI	GDGCHC	1 cut(s)	78
SetI	ASST	9 cut(s)	78, 99, 111, 255, 337, 438, 603, 622, 677
SfaNI	GCATC	3 cut(s)	42, 484, 736
SfcI	CTRYAG	2 cut(s)	186, 512
SinI	GGWCC	2 cut(s)	451, 601
SmaI	CCCGGG	1 cut(s)	528
SmiMI	CAYNNNNRTG	2 cut(s)	726, 741
SpeI	ACTAGT	1 cut(s)	146
Sse9I	AATT	1 cut(s)	127
SseBI	AGGCCT	1 cut(s)	634
SsiI	CCGC	6 cut(s)	81, 113, 419, 467, 531, 549
SspMI	CTAG	3 cut(s)	147, 560, 666
SstI	GAGCTC	1 cut(s)	78
StuI	AGGCCT	1 cut(s)	634
StyD4I	CCNGG	5 cut(s)	402, 470, 525, 526, 638
TaaI	ACNGT	1 cut(s)	324
TaiI	ACGT	3 cut(s)	99, 337, 438
TaqI	TCGA	2 cut(s)	341, 399
TasI	AATT	1 cut(s)	127
TatI	WGTACW	1 cut(s)	15
TauI	GCSGC	3 cut(s)	115, 421, 470
TfiI	GAWTC	1 cut(s)	407
Tru1I	TTAA	2 cut(s)	414, 751
Tru9I	TTAA	2 cut(s)	414, 751
TscAI	CASTG	1 cut(s)	169
TseFI	GTSAC	1 cut(s)	324
TseI	GCWGC	5 cut(s)	347, 377, 385, 620, 733
Tsp45I	GTSAC	1 cut(s)	324
TspDTI	ATGAA	1 cut(s)	267
TspMI	CCCGGG	1 cut(s)	526
TspRI	CASTG	1 cut(s)	169
Van91I	CCANNNNNTGG	1 cut(s)	610
VpaK11BI	GGWCC	2 cut(s)	451, 601
XmaI	CCCGGG	1 cut(s)	526
XspI	CTAG	3 cut(s)	147, 560, 666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.