Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0004140

Physical Location & Seq

Genomic Coordinates Forward (+)

8333147 .. 8333893

Gene Structure 747 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1098800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 747 bp

ATGGATATTGGCCAGTACAAATCAGAGGAGCAGATGGAGATGACGATGATGATGATACAAATGGAAAAAATCTCTGAGCTCTGCGGTGCCTACAACAACGATGTGGTTTCCGACCTGCCGCCGTCCGATCACAATTTCTCATCCACCACAACTAGTCCTAATAGTCTCAAGGCCACCATGTCACATTACACCGACCAAAACACACATAACAATAATATTCATTCACCAAACTCACAATCCTTCCTAAACCTCCCAACAATCCCATCTACCGTTTCATTCACCAACACAAACCCTACTGACCAAACAAACGGCACGTCACCGTCGCAAAAACGTGCTTCGATGGCAGCCATGAGGGAGATGATATTCCGGATTGCAGCCATGCAGCCGATCAACATCGACCCAGAATCCGTTAAGCCACCAAAGAGAAGGAACGTGAAGATTTCTAAGGACCCTCAGAGCGTGGCGGCCCGGCATCGGAGGGAGAGGATTAGTGAGAGGATTAGAATTCTGCAGAGACTTGTCCCGGGCGGGACAAAAATGGACACCGCTTCCATGCTAGATGAGGCCATTCACTATGTCAAGTTTTTGAAGACTCAGGTCCAAACGCTGGAGAGAGCTGCGACTAATAGGCCTACCGGGATTGGATTTCCTGTGGCCATGTCTAGTCGGAGCTACATTTCTAACTCTATGTCTGAGGTTTACGAAGCTTCTCATCATCATCAGAATGTGCAGCATTTCGATCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

249

Amino Acids

28.0

Weight (kDa)

8.99

Isoelectric Point (pI)

57.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	156 - 197	3.4e-07	Helix-loop-helix DNA-binding domain
bHLH_SAC51	PF23173	158 - 202	9.1e-12	Transcription factor SAC51 bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004140)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G50330 AT5G67060 AT5G67060
fragaria_vesca	FvH4_1g16130 FvH4_2g34280
malus_domestica	MD02G1173400.v1.1 MD08G1098800.v1.1 MD15G1081100.v1.1 MD15G1284900.v1.1
prunus_persica	Prupe.1G434000_v2.0.a1 Prupe.7G132300_v2.0.a1
pyrus_communis	pycom15g07560 pycom15g07580
rosa_chinensis	RchiOBHm_Chr2g0105811 RchiOBHm_Chr6g0308101
rosa_laevigata	RLG00000010668 RLG00000017461
rosa_multiflora	Rmu_sc0005706.1_g000001 Rmu_sc0010497.1_g000002
rosa_roxburghii	Rroxscaffold_2G00137880 Rroxscaffold_7G00160540
rosa_rugosa	Rorug02G0130500 Rorug06G0364000
rosa_samantha	Rh2AG181700 Rh2BG191300 Rh2CG186000 Rh2DG187900 Rh6AG472700 Rh6BG483900 Rh6CG488800 Rh6DG475100
rosa_wichuraiana	Rw2G014230 Rw6G041250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	123
AccB1I	GGYRCC	1 cut(s)	86
AccB7I	CCANNNNNTGG	1 cut(s)	607
AccIII	TCCGGA	1 cut(s)	366
AciI	CCGC	5 cut(s)	84, 119, 464, 528, 546
AcoI	YGGCCR	2 cut(s)	10, 654
AcsI	RAATTY	1 cut(s)	504
AfaI	GTAC	1 cut(s)	17
AfiI	CCNNNNNNNGG	2 cut(s)	474, 607
AgsI	TTSAA	1 cut(s)	589
AhlI	ACTAGT	1 cut(s)	152
AjiI	CACGTC	1 cut(s)	315
AluBI	AGCT	4 cut(s)	79, 617, 672, 707
AluI	AGCT	4 cut(s)	79, 617, 672, 707
Alw21I	GWGCWC	1 cut(s)	81
Alw26I	GTCTC	2 cut(s)	170, 508
Ama87I	CYCGRG	1 cut(s)	523
Aor13HI	TCCGGA	1 cut(s)	366
AoxI	GGCC	6 cut(s)	10, 171, 465, 564, 629, 654
ApeKI	GCWGC	5 cut(s)	344, 374, 382, 617, 730
ApoI	RAATTY	1 cut(s)	504
ArsI	GACNNNNNNTTYG	2 cut(s)	299, 331
AspS9I	GGNCC	3 cut(s)	448, 466, 598
AsuC2I	CCSGG	4 cut(s)	469, 524, 525, 637
AsuHPI	GGTGA	3 cut(s)	216, 271, 309
AvaI	CYCGRG	1 cut(s)	523
AvaII	GGWCC	2 cut(s)	448, 598
BalI	TGGCCA	2 cut(s)	12, 656
BanI	GGYRCC	1 cut(s)	86
BanII	GRGCYC	1 cut(s)	81
BbsI	GAAGAC	1 cut(s)	596
Bbv12I	GWGCWC	1 cut(s)	81
BbvI	GCAGC	5 cut(s)	356, 386, 394, 604, 742
BccI	CCATC	3 cut(s)	28, 271, 334
BceAI	ACGGC	2 cut(s)	106, 325
BcgI	CGANNNNNNTGC	1 cut(s)	722
BcnI	CCSGG	4 cut(s)	469, 524, 525, 637
BcoDI	GTCTC	2 cut(s)	170, 508
BcuI	ACTAGT	1 cut(s)	152
BfaI	CTAG	3 cut(s)	153, 557, 663
BfmI	CTRYAG	1 cut(s)	509
BfuAI	ACCTGC	1 cut(s)	123
BisI	GCNGC	7 cut(s)	119, 345, 375, 383, 465, 618, 731
BlsI	GCNGC	7 cut(s)	120, 346, 376, 384, 466, 619, 732
Bme1390I	CCNGG	4 cut(s)	469, 524, 525, 637
Bme18I	GGWCC	2 cut(s)	448, 598
BmeT110I	CYCGRG	1 cut(s)	523
BmgBI	CACGTC	1 cut(s)	315
BmgT120I	GGNCC	3 cut(s)	448, 466, 598
BmiI	GGNNCC	2 cut(s)	88, 450
BmrFI	CCNGG	4 cut(s)	469, 524, 525, 637
BmsI	GCATC	1 cut(s)	481
BoxI	GACNNNNGTC	1 cut(s)	596
BpiI	GAAGAC	1 cut(s)	596
BpmI	CTGGAG	1 cut(s)	629
BpuEI	CTTGAG	1 cut(s)	152
BpuMI	CCSGG	4 cut(s)	469, 524, 525, 637
BsaBI	GATNNNNATC	1 cut(s)	392
BsaJI	CCNNGG	1 cut(s)	523
BsaWI	WCCGGW	1 cut(s)	366
Bsc4I	CCNNNNNNNGG	2 cut(s)	474, 607
Bse1I	ACTGG	1 cut(s)	13
Bse8I	GATNNNNATC	1 cut(s)	392
BseAI	TCCGGA	1 cut(s)	366
BseDI	CCNNGG	1 cut(s)	523
BseGI	GGATG	1 cut(s)	140
BseJI	GATNNNNATC	1 cut(s)	392
BseLI	CCNNNNNNNGG	2 cut(s)	474, 607
BseMII	CTCAG	4 cut(s)	66, 467, 608, 684
BseNI	ACTGG	1 cut(s)	13
BseRI	GAGGAG	1 cut(s)	41
BseXI	GCAGC	5 cut(s)	356, 386, 394, 604, 742
Bsh1285I	CGRYCG	1 cut(s)	742
BshFI	GGCC	6 cut(s)	12, 173, 467, 566, 631, 656
BshNI	GGYRCC	1 cut(s)	86
BsiEI	CGRYCG	1 cut(s)	742
BsiHKAI	GWGCWC	1 cut(s)	81
BsiHKCI	CYCGRG	1 cut(s)	523
BsiSI	CCGG	4 cut(s)	367, 469, 524, 636
BslFI	GGGAC	2 cut(s)	506, 544
BslI	CCNNNNNNNGG	2 cut(s)	474, 607
BsmAI	GTCTC	2 cut(s)	170, 508
BsmFI	GGGAC	2 cut(s)	506, 544
BsnI	GGCC	6 cut(s)	12, 173, 467, 566, 631, 656
BsoBI	CYCGRG	1 cut(s)	523
Bsp1286I	GDGCHC	1 cut(s)	81
Bsp13I	TCCGGA	1 cut(s)	366
Bsp143I	GATC	3 cut(s)	127, 387, 739
BspACI	CCGC	5 cut(s)	84, 119, 464, 528, 546
BspANI	GGCC	6 cut(s)	12, 173, 467, 566, 631, 656
BspCNI	CTCAG	4 cut(s)	67, 466, 607, 685
BspEI	TCCGGA	1 cut(s)	366
BspLI	GGNNCC	2 cut(s)	88, 450
BspMAI	CTGCAG	1 cut(s)	513
BspMI	ACCTGC	1 cut(s)	123
BspT107I	GGYRCC	1 cut(s)	86
BsrI	ACTGG	1 cut(s)	13
BssECI	CCNNGG	1 cut(s)	523
BssMI	GATC	3 cut(s)	127, 387, 739
Bst4CI	ACNGT	2 cut(s)	271, 321
BstDEI	CTNAG	5 cut(s)	75, 444, 453, 594, 693
BstF5I	GGATG	1 cut(s)	140
BstKTI	GATC	3 cut(s)	130, 390, 742
BstMAI	GTCTC	2 cut(s)	170, 508
BstMBI	GATC	3 cut(s)	127, 387, 739
BstMCI	CGRYCG	1 cut(s)	742
BstMWI	GCNNNNNNNGC	1 cut(s)	341
BstPAI	GACNNNNGTC	1 cut(s)	596
BstSCI	CCNGG	4 cut(s)	467, 522, 523, 635
BstSFI	CTRYAG	1 cut(s)	509
BstV1I	GCAGC	5 cut(s)	356, 386, 394, 604, 742
BstV2I	GAAGAC	1 cut(s)	596
BsuRI	GGCC	6 cut(s)	12, 173, 467, 566, 631, 656
BtrI	CACGTC	1 cut(s)	315
BtsCI	GGATG	1 cut(s)	140
BveI	ACCTGC	1 cut(s)	123
Cfr13I	GGNCC	3 cut(s)	448, 466, 598
Cfr9I	CCCGGG	1 cut(s)	523
Csp6I	GTAC	1 cut(s)	16
CviAII	CATG	5 cut(s)	178, 349, 379, 553, 658
CviQI	GTAC	1 cut(s)	16
DdeI	CTNAG	5 cut(s)	75, 444, 453, 594, 693
DpnI	GATC	3 cut(s)	129, 389, 741
DpnII	GATC	3 cut(s)	127, 387, 739
EaeI	YGGCCR	2 cut(s)	10, 654
Ecl136II	GAGCTC	1 cut(s)	79
Eco147I	AGGCCT	1 cut(s)	631
Eco24I	GRGCYC	1 cut(s)	81
Eco47I	GGWCC	2 cut(s)	448, 598
Eco53kI	GAGCTC	1 cut(s)	79
Eco88I	CYCGRG	1 cut(s)	523
EcoICRI	GAGCTC	1 cut(s)	79
EcoO109I	RGGNCCY	1 cut(s)	448
EcoRI	GAATTC	1 cut(s)	504
EcoT38I	GRGCYC	1 cut(s)	81
FaeI	CATG	5 cut(s)	181, 352, 382, 556, 661
FaiI	YATR	8 cut(s)	179, 207, 350, 380, 554, 576, 659, 689
FaqI	GGGAC	2 cut(s)	506, 544
FatI	CATG	5 cut(s)	177, 348, 378, 552, 657
FauI	CCCGC	1 cut(s)	521
Fnu4HI	GCNGC	7 cut(s)	119, 345, 375, 383, 465, 618, 731
FokI	GGATG	1 cut(s)	127
FriOI	GRGCYC	1 cut(s)	81
Fsp4HI	GCNGC	7 cut(s)	119, 345, 375, 383, 465, 618, 731
FspBI	CTAG	3 cut(s)	153, 557, 663
GluI	GCNGC	7 cut(s)	119, 345, 375, 383, 465, 618, 731
GsuI	CTGGAG	1 cut(s)	629
HaeIII	GGCC	6 cut(s)	12, 173, 467, 566, 631, 656
HapII	CCGG	4 cut(s)	367, 469, 524, 636
Hin1II	CATG	5 cut(s)	181, 352, 382, 556, 661
HindIII	AAGCTT	1 cut(s)	705
HinfI	GANTC	2 cut(s)	404, 592
HpaII	CCGG	4 cut(s)	367, 469, 524, 636
HphI	GGTGA	3 cut(s)	216, 271, 309
Hpy166II	GTNNAC	1 cut(s)	700
Hpy188I	TCNGA	9 cut(s)	25, 76, 112, 127, 456, 477, 669, 694, 723
Hpy188III	TCNNGA	1 cut(s)	367
Hpy8I	GTNNAC	1 cut(s)	700
Hpy99I	CGWCG	1 cut(s)	325
HpyAV	CCTTC	2 cut(s)	250, 420
HpyCH4III	ACNGT	2 cut(s)	271, 321
HpyCH4IV	ACGT	3 cut(s)	314, 331, 432
HpyCH4V	TGCA	4 cut(s)	374, 382, 511, 730
HpyF10VI	GCNNNNNNNGC	1 cut(s)	341
HpyF3I	CTNAG	5 cut(s)	75, 444, 453, 594, 693
HpySE526I	ACGT	3 cut(s)	314, 331, 432
Hsp92II	CATG	5 cut(s)	181, 352, 382, 556, 661
Kpn2I	TCCGGA	1 cut(s)	366
Kzo9I	GATC	3 cut(s)	127, 387, 739
LmnI	GCTCC	2 cut(s)	28, 669
Lsp1109I	GCAGC	5 cut(s)	356, 386, 394, 604, 742
LweI	GCATC	1 cut(s)	481
MaeI	CTAG	3 cut(s)	153, 557, 663
MaeII	ACGT	3 cut(s)	314, 331, 432
MaeIII	GTNAC	2 cut(s)	180, 315
MalI	GATC	3 cut(s)	129, 389, 741
MboI	GATC	3 cut(s)	127, 387, 739
MboII	GAAGA	2 cut(s)	448, 601
MhlI	GDGCHC	1 cut(s)	81
MlsI	TGGCCA	2 cut(s)	12, 656
MluCI	AATT	2 cut(s)	133, 504
MluNI	TGGCCA	2 cut(s)	12, 656
MlyI	GAGTC	1 cut(s)	586
MmeI	TCCRAC	2 cut(s)	135, 647
MnlI	CCTC	9 cut(s)	19, 260, 345, 462, 471, 477, 489, 556, 688
Mox20I	TGGCCA	2 cut(s)	12, 656
MroI	TCCGGA	1 cut(s)	366
MscI	TGGCCA	2 cut(s)	12, 656
MseI	TTAA	1 cut(s)	411
MslI	CAYNNNNRTG	1 cut(s)	723
Msp20I	TGGCCA	2 cut(s)	12, 656
MspI	CCGG	4 cut(s)	367, 469, 524, 636
MspR9I	CCNGG	4 cut(s)	469, 524, 525, 637
MwoI	GCNNNNNNNGC	1 cut(s)	341
NciI	CCSGG	4 cut(s)	469, 524, 525, 637
NdeII	GATC	3 cut(s)	127, 387, 739
NlaIII	CATG	5 cut(s)	181, 352, 382, 556, 661
NlaIV	GGNNCC	2 cut(s)	88, 450
NmuCI	GTSAC	2 cut(s)	180, 315
PceI	AGGCCT	1 cut(s)	631
PfeI	GAWTC	1 cut(s)	404
PflMI	CCANNNNNTGG	1 cut(s)	607
PkrI	GCNGC	7 cut(s)	120, 346, 376, 384, 466, 619, 732
Ple19I	CGATCG	1 cut(s)	742
PleI	GAGTC	1 cut(s)	586
PpsI	GAGTC	1 cut(s)	586
PpuMI	RGGWCCY	1 cut(s)	448
PshAI	GACNNNNGTC	1 cut(s)	596
Psp124BI	GAGCTC	1 cut(s)	81
Psp5II	RGGWCCY	1 cut(s)	448
PspN4I	GGNNCC	2 cut(s)	88, 450
PspPI	GGNCC	3 cut(s)	448, 466, 598
PspPPI	RGGWCCY	1 cut(s)	448
PstI	CTGCAG	1 cut(s)	513
PvuI	CGATCG	1 cut(s)	742
RsaI	GTAC	1 cut(s)	17
RsaNI	GTAC	1 cut(s)	16
RseI	CAYNNNNRTG	1 cut(s)	723
SacI	GAGCTC	1 cut(s)	81
SaqAI	TTAA	1 cut(s)	411
SatI	GCNGC	7 cut(s)	119, 345, 375, 383, 465, 618, 731
Sau3AI	GATC	3 cut(s)	127, 387, 739
Sau96I	GGNCC	3 cut(s)	448, 466, 598
SchI	GAGTC	1 cut(s)	586
ScrFI	CCNGG	4 cut(s)	469, 524, 525, 637
SduI	GDGCHC	1 cut(s)	81
SfaNI	GCATC	1 cut(s)	481
SfcI	CTRYAG	1 cut(s)	509
SinI	GGWCC	2 cut(s)	448, 598
SmaI	CCCGGG	1 cut(s)	525
SmiMI	CAYNNNNRTG	1 cut(s)	723
SmlI	CTYRAG	1 cut(s)	167
SmoI	CTYRAG	1 cut(s)	167
SpeI	ACTAGT	1 cut(s)	152
Sse9I	AATT	2 cut(s)	133, 504
SseBI	AGGCCT	1 cut(s)	631
SsiI	CCGC	5 cut(s)	84, 119, 464, 528, 546
SspI	AATATT	1 cut(s)	217
SspMI	CTAG	3 cut(s)	153, 557, 663
SstI	GAGCTC	1 cut(s)	81
StuI	AGGCCT	1 cut(s)	631
StyD4I	CCNGG	4 cut(s)	467, 522, 523, 635
TaaI	ACNGT	2 cut(s)	271, 321
TaiI	ACGT	3 cut(s)	317, 334, 435
TaqI	TCGA	3 cut(s)	338, 396, 738
TasI	AATT	2 cut(s)	133, 504
TatI	WGTACW	1 cut(s)	15
TauI	GCSGC	2 cut(s)	121, 467
TfiI	GAWTC	1 cut(s)	404
Tru1I	TTAA	1 cut(s)	411
Tru9I	TTAA	1 cut(s)	411
TseFI	GTSAC	2 cut(s)	180, 315
TseI	GCWGC	5 cut(s)	344, 374, 382, 617, 730
Tsp45I	GTSAC	2 cut(s)	180, 315
TspDTI	ATGAA	2 cut(s)	209, 264
TspGWI	ACGGA	1 cut(s)	397
TspMI	CCCGGG	1 cut(s)	523
Van91I	CCANNNNNTGG	1 cut(s)	607
VpaK11BI	GGWCC	2 cut(s)	448, 598
XapI	RAATTY	1 cut(s)	504
XmaI	CCCGGG	1 cut(s)	523
XspI	CTAG	3 cut(s)	153, 557, 663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.