AT4G10030

Alpha beta

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006510
Physical Location & Seq
Reverse (-)
6270241 .. 6273442
3202 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10030.1

Sequence Viewer

Length: 1143 bp
ATGTCGGCGGTCTCGTGCTCCACGGCGAGTTCTAGCGGAGGATGTGCATTTAGGTTTCGATCAAAGACATCAATCCATTCTCCTAATTTCATCAGAATTGGGACAAATTCTCAGAGTGACAGATTCTGTAATCATCGTTTGGAATTGGTACATGGAACTCGTAAAGTGAAAGGAGCATCTGTACGTATGGCATTAGTTGAGGAGAGACAATCAACGAGCAAAGATGTTGCTGAGCCTCCTCGGGTCCTGGCGTATGATCTTGTTCAAGGAGCACTTGTGAAATGGAGATGGAAGGATGACAAATCTGTTCCAGATACACCTACTGCTGTTCTCCTACACGGAATTTTAGGGAGCGGGAAAAACTGGGGCACTTTTGCTCGTAGATTGGCTCATGAATTCCCAACGTGGCAGTTTCTATTGGTAGACCTGCGTTGCCATGGGGATTCAACATCTCTCAAGAAGAGAGGTCCACACTCGGTTGCTACTACTGCTTCTGATGTTCTAAAACTTGTTGGTCAGTTGAGGTTAACACCTCGGGTCCTTGTTGGTCATAGCTTTGGGGGGAAAGTTGTATTAAGCATGGTAGAGCAAGCAGCTAAGCCTCTTCCACGACCAGTCCGAGCTTGGGTATTGGATGCTACTCCTGGTAAAGTTCGTGCAGGAGGAGATGGAGAAGATCATCCACGAGAGCTTATATCATTTCTACGTAAATTGCCAAAAGTGGTCTTGTCAAAGCGCGAGGTTCTTAAAGCTCTTATAAATGAAGGGTTTTCCAATGATGTCGCACAGTGGGTCATTACCAATCTCAGACCTACTGGACCATCAGCTTCTAGCTTCTCGTGGACATTTGACCTAGACGGGATCGCTGAACTTTACCAGTCTTACGAAGAGACAAATCTATGGAACTTTGTTGAGAATCTTCCAAGAGGAGTACATGTGAATTTTCTGAAAGCGGAAAGAAGCTTGCACCGTTGGGCCCTAGAAGACCTTCAGCGAATCCACGCCGCTGAAGAGCTAGCCTCTGAAGAAGGCGGTGGTGTAGAAATGCATGTCTTGGAAGATGCCGGTCATTGGGTTCACACGGATAACCCGGATGGTCTCTTCAGGATCTTGTCATCTTCTTTCCAGGTTCTCAGAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_6

Protein Analysis

380

Amino Acids

42.15

Weight (kDa)

9.26

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_6 PF12697 110 - 368 1.7e-20 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 110 - 228 1.7e-15 alpha/beta hydrolase fold
Hydrolase_4 PF12146 110 - 252 1.9e-07 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
Acc36I ACCTGC 1 cut(s) 435
AccBSI CCGCTC 1 cut(s) 354
AccI GTMKAC 1 cut(s) 423
AccII CGCG 1 cut(s) 738
AciI CCGC 6 cut(s) 8, 36, 354, 953, 1005, 1032
AclWI GGATC 2 cut(s) 869, 1115
AcsI RAATTY 4 cut(s) 106, 342, 395, 940
AcuI CTGAAG 4 cut(s) 974, 1029, 1044, 1087
AfaI GTAC 3 cut(s) 150, 183, 933
AfiI CCNNNNNNNGG 2 cut(s) 625, 1071
AflIII ACRYGT 1 cut(s) 934
AgsI TTSAA 2 cut(s) 266, 447
AjnI CCWGG 3 cut(s) 246, 643, 1125
AluBI AGCT 9 cut(s) 555, 596, 623, 691, 752, 827, 834, 963, 1015
AluI AGCT 9 cut(s) 555, 596, 623, 691, 752, 827, 834, 963, 1015
Alw21I GWGCWC 2 cut(s) 20, 274
Alw26I GTCTC 4 cut(s) 16, 199, 884, 1103
AlwI GGATC 2 cut(s) 869, 1115
AlwNI CAGNNNCTG 1 cut(s) 126
Ama87I CYCGRG 2 cut(s) 240, 534
AoxI GGCC 1 cut(s) 975
ApaI GGGCCC 1 cut(s) 979
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 4 cut(s) 106, 342, 395, 940
Asp700I GAANNNNTTC 1 cut(s) 987
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 6 cut(s) 244, 467, 538, 818, 975, 976
AsuC2I CCSGG 1 cut(s) 1091
AsuNHI GCTAGC 1 cut(s) 1015
AvaI CYCGRG 2 cut(s) 240, 534
AvaII GGWCC 4 cut(s) 244, 467, 538, 818
BaeGI GKGCMC 2 cut(s) 371, 979
BanII GRGCYC 1 cut(s) 979
BauI CACGAG 3 cut(s) 13, 684, 838
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 20, 274
BbvI GCAGC 1 cut(s) 605
BccI CCATC 4 cut(s) 282, 662, 829, 1088
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 3 cut(s) 248, 645, 1127
BcnI CCSGG 1 cut(s) 1091
BcoDI GTCTC 4 cut(s) 16, 199, 884, 1103
BfaI CTAG 6 cut(s) 33, 831, 854, 980, 1016, 1141
BfuAI ACCTGC 1 cut(s) 435
BisI GCNGC 2 cut(s) 594, 1005
BlpI GCTNAGC 2 cut(s) 231, 597
BlsI GCNGC 2 cut(s) 595, 1006
Bme1390I CCNGG 4 cut(s) 248, 645, 1091, 1127
Bme18I GGWCC 4 cut(s) 244, 467, 538, 818
BmeT110I CYCGRG 2 cut(s) 240, 534
BmgT120I GGNCC 6 cut(s) 244, 467, 538, 818, 975, 976
BmiI GGNNCC 3 cut(s) 245, 539, 977
BmrFI CCNGG 4 cut(s) 248, 645, 1091, 1127
BmrI ACTGGG 1 cut(s) 373
BmsI GCATC 3 cut(s) 185, 625, 1051
BmtI GCTAGC 1 cut(s) 1019
BmuI ACTGGG 1 cut(s) 373
BpiI GAAGAC 1 cut(s) 990
BplI GAGNNNNNCTC 2 cut(s) 1004, 1036
Bpu1102I GCTNAGC 2 cut(s) 231, 597
BpuEI CTTGAG 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 1091
BsaAI YACGTR 2 cut(s) 185, 707
BsaI GGTCTC 2 cut(s) 16, 1103
BsaJI CCNNGG 4 cut(s) 21, 239, 436, 533
BsaXI ACNNNNNCTCC 6 cut(s) 165, 195, 261, 291, 921, 951
Bsc4I CCNNNNNNNGG 2 cut(s) 625, 1071
Bse118I RCCGGY 1 cut(s) 1064
Bse1I ACTGG 4 cut(s) 368, 614, 820, 877
BseBI CCWGG 3 cut(s) 248, 645, 1127
BseDI CCNNGG 4 cut(s) 21, 239, 436, 533
BseGI GGATG 5 cut(s) 47, 301, 640, 679, 1099
BseLI CCNNNNNNNGG 2 cut(s) 625, 1071
BseMII CTCAG 3 cut(s) 125, 222, 820
BseNI ACTGG 4 cut(s) 368, 614, 820, 877
BseRI GAGGAG 4 cut(s) 215, 228, 678, 942
BseSI GKGCMC 2 cut(s) 371, 979
BseXI GCAGC 1 cut(s) 605
BsgI GTGCAG 1 cut(s) 678
Bsh1236I CGCG 1 cut(s) 738
BshFI GGCC 1 cut(s) 977
BsiHKAI GWGCWC 2 cut(s) 20, 274
BsiHKCI CYCGRG 2 cut(s) 240, 534
BsiSI CCGG 2 cut(s) 1065, 1091
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 625, 1071
BsmAI GTCTC 4 cut(s) 16, 199, 884, 1103
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 1 cut(s) 977
Bso31I GGTCTC 2 cut(s) 16, 1103
BsoBI CYCGRG 2 cut(s) 240, 534
Bsp120I GGGCCC 1 cut(s) 975
Bsp1286I GDGCHC 4 cut(s) 20, 274, 371, 979
Bsp143I GATC 5 cut(s) 59, 256, 676, 861, 1107
Bsp1720I GCTNAGC 2 cut(s) 231, 597
Bsp19I CCATGG 1 cut(s) 436
BspACI CCGC 6 cut(s) 8, 36, 354, 953, 1005, 1032
BspANI GGCC 1 cut(s) 977
BspCNI CTCAG 3 cut(s) 124, 223, 819
BspFNI CGCG 1 cut(s) 738
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 3 cut(s) 245, 539, 977
BspMI ACCTGC 1 cut(s) 435
BspOI GCTAGC 1 cut(s) 1019
BspPI GGATC 2 cut(s) 869, 1115
BspQI GCTCTTC 1 cut(s) 1005
BspTNI GGTCTC 2 cut(s) 16, 1103
BsrBI CCGCTC 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 1064
BsrI ACTGG 4 cut(s) 368, 614, 820, 877
BssAI RCCGGY 1 cut(s) 1064
BssECI CCNNGG 4 cut(s) 21, 239, 436, 533
BssMI GATC 5 cut(s) 59, 256, 676, 861, 1107
BssSI CACGAG 3 cut(s) 13, 684, 838
BssT1I CCWWGG 1 cut(s) 436
Bst2BI CACGAG 3 cut(s) 13, 684, 838
Bst2UI CCWGG 3 cut(s) 248, 645, 1127
Bst4CI ACNGT 2 cut(s) 789, 971
Bst6I CTCTTC 5 cut(s) 455, 609, 882, 1005, 1106
BstBAI YACGTR 2 cut(s) 185, 707
BstC8I GCNNGC 3 cut(s) 591, 965, 1017
BstDEI CTNAG 5 cut(s) 111, 231, 597, 806, 1133
BstDSI CCRYGG 2 cut(s) 21, 436
BstF5I GGATG 5 cut(s) 47, 301, 640, 679, 1099
BstFNI CGCG 1 cut(s) 738
BstHHI GCGC 1 cut(s) 738
BstKTI GATC 5 cut(s) 62, 259, 679, 864, 1110
BstMAI GTCTC 4 cut(s) 16, 199, 884, 1103
BstMBI GATC 5 cut(s) 59, 256, 676, 861, 1107
BstMWI GCNNNNNNNGC 1 cut(s) 488
BstNI CCWGG 3 cut(s) 248, 645, 1127
BstNSI RCATGY 2 cut(s) 938, 1052
BstSCI CCNGG 4 cut(s) 246, 643, 1089, 1125
BstSLI GKGCMC 2 cut(s) 371, 979
BstSNI TACGTA 2 cut(s) 185, 707
BstUI CGCG 1 cut(s) 738
BstV1I GCAGC 1 cut(s) 605
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 1107
BstYI RGATCY 1 cut(s) 1107
BsuRI GGCC 1 cut(s) 977
BtgI CCRYGG 2 cut(s) 21, 436
BtsCI GGATG 5 cut(s) 47, 301, 640, 679, 1099
BtsIMutI CAGTG 1 cut(s) 794
BveI ACCTGC 1 cut(s) 435
Cac8I GCNNGC 3 cut(s) 591, 965, 1017
CaiI CAGNNNCTG 1 cut(s) 126
CciI TCATGA 1 cut(s) 391
CfoI GCGC 1 cut(s) 738
Cfr10I RCCGGY 1 cut(s) 1064
Cfr13I GGNCC 6 cut(s) 244, 467, 538, 818, 975, 976
Csp6I GTAC 3 cut(s) 149, 182, 932
CviAII CATG 6 cut(s) 152, 392, 437, 580, 935, 1049
CviQI GTAC 3 cut(s) 149, 182, 932
DdeI CTNAG 5 cut(s) 111, 231, 597, 806, 1133
DpnI GATC 5 cut(s) 61, 258, 678, 863, 1109
DpnII GATC 5 cut(s) 59, 256, 676, 861, 1107
Eam1104I CTCTTC 5 cut(s) 455, 609, 882, 1005, 1106
EarI CTCTTC 5 cut(s) 455, 609, 882, 1005, 1106
Eco105I TACGTA 2 cut(s) 185, 707
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 979
Eco31I GGTCTC 2 cut(s) 16, 1103
Eco47I GGWCC 4 cut(s) 244, 467, 538, 818
Eco57I CTGAAG 4 cut(s) 974, 1029, 1044, 1087
Eco88I CYCGRG 2 cut(s) 240, 534
EcoO109I RGGNCCY 3 cut(s) 244, 538, 976
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 3 cut(s) 246, 643, 1125
EcoT14I CCWWGG 1 cut(s) 436
EcoT22I ATGCAT 1 cut(s) 1050
EcoT38I GRGCYC 1 cut(s) 979
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 6 cut(s) 155, 395, 440, 583, 938, 1052
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FaqI GGGAC 1 cut(s) 115
FatI CATG 6 cut(s) 151, 391, 436, 579, 934, 1048
FauI CCCGC 1 cut(s) 347
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 594, 1005
FokI GGATG 5 cut(s) 54, 308, 647, 666, 1106
FriOI GRGCYC 1 cut(s) 979
Fsp4HI GCNGC 2 cut(s) 594, 1005
FspBI CTAG 6 cut(s) 33, 831, 854, 980, 1016, 1141
GlaI GCGC 1 cut(s) 737
GluI GCNGC 2 cut(s) 594, 1005
HaeIII GGCC 1 cut(s) 977
HapII CCGG 2 cut(s) 1065, 1091
HhaI GCGC 1 cut(s) 738
Hin1II CATG 6 cut(s) 155, 395, 440, 583, 938, 1052
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HincII GTYRAC 1 cut(s) 528
HindII GTYRAC 1 cut(s) 528
HindIII AAGCTT 1 cut(s) 961
HinfI GANTC 4 cut(s) 123, 443, 916, 996
HpaI GTTAAC 1 cut(s) 528
HpaII CCGG 2 cut(s) 1065, 1091
Hpy166II GTNNAC 5 cut(s) 424, 470, 528, 843, 1078
Hpy188I TCNGA 8 cut(s) 95, 114, 496, 620, 809, 948, 1024, 1136
Hpy188III TCNNGA 4 cut(s) 311, 392, 457, 1105
Hpy8I GTNNAC 5 cut(s) 424, 470, 528, 843, 1078
HpyAV CCTTC 4 cut(s) 286, 758, 998, 1022
HpyCH4III ACNGT 2 cut(s) 789, 971
HpyCH4IV ACGT 3 cut(s) 184, 404, 706
HpyCH4V TGCA 4 cut(s) 47, 659, 967, 1048
HpyF10VI GCNNNNNNNGC 1 cut(s) 488
HpyF3I CTNAG 5 cut(s) 111, 231, 597, 806, 1133
HpySE526I ACGT 3 cut(s) 184, 404, 706
Hsp92II CATG 6 cut(s) 155, 395, 440, 583, 938, 1052
HspAI GCGC 1 cut(s) 736
KspAI GTTAAC 1 cut(s) 528
Kzo9I GATC 5 cut(s) 59, 256, 676, 861, 1107
LguI GCTCTTC 1 cut(s) 1005
LmnI GCTCC 4 cut(s) 23, 173, 269, 351
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 3 cut(s) 185, 625, 1051
MaeI CTAG 6 cut(s) 33, 831, 854, 980, 1016, 1141
MaeII ACGT 3 cut(s) 184, 404, 706
MaeIII GTNAC 1 cut(s) 116
MalI GATC 5 cut(s) 61, 258, 678, 863, 1109
MbiI CCGCTC 1 cut(s) 354
MboI GATC 5 cut(s) 59, 256, 676, 861, 1107
MflI RGATCY 1 cut(s) 1107
MhlI GDGCHC 4 cut(s) 20, 274, 371, 979
MluCI AATT 8 cut(s) 85, 96, 106, 143, 342, 395, 710, 940
Mph1103I ATGCAT 1 cut(s) 1050
MroXI GAANNNNTTC 1 cut(s) 987
MseI TTAA 3 cut(s) 527, 575, 747
MspA1I CMGCKG 1 cut(s) 1007
MspI CCGG 2 cut(s) 1065, 1091
MspR9I CCNGG 4 cut(s) 248, 645, 1091, 1127
MvaI CCWGG 3 cut(s) 248, 645, 1127
MvnI CGCG 1 cut(s) 738
MwoI GCNNNNNNNGC 1 cut(s) 488
NciI CCSGG 1 cut(s) 1091
NcoI CCATGG 1 cut(s) 436
NdeII GATC 5 cut(s) 59, 256, 676, 861, 1107
NheI GCTAGC 1 cut(s) 1015
NlaIII CATG 6 cut(s) 155, 395, 440, 583, 938, 1052
NlaIV GGNNCC 3 cut(s) 245, 539, 977
NmuCI GTSAC 1 cut(s) 116
NsiI ATGCAT 1 cut(s) 1050
NspI RCATGY 2 cut(s) 938, 1052
PagI TCATGA 1 cut(s) 391
PciI ACATGT 1 cut(s) 934
PciSI GCTCTTC 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 616
PdmI GAANNNNTTC 1 cut(s) 987
PfeI GAWTC 4 cut(s) 123, 443, 916, 996
PkrI GCNGC 2 cut(s) 595, 1006
Ppu21I YACGTR 2 cut(s) 185, 707
PpuMI RGGWCCY 2 cut(s) 244, 538
PscI ACATGT 1 cut(s) 934
PsiI TTATAA 1 cut(s) 758
Psp5II RGGWCCY 2 cut(s) 244, 538
Psp6I CCWGG 3 cut(s) 246, 643, 1125
PspGI CCWGG 3 cut(s) 246, 643, 1125
PspN4I GGNNCC 3 cut(s) 245, 539, 977
PspOMI GGGCCC 1 cut(s) 975
PspPI GGNCC 6 cut(s) 244, 467, 538, 818, 975, 976
PspPPI RGGWCCY 2 cut(s) 244, 538
PstNI CAGNNNCTG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 1107
RsaI GTAC 3 cut(s) 150, 183, 933
RsaNI GTAC 3 cut(s) 149, 182, 932
SapI GCTCTTC 1 cut(s) 1005
SaqAI TTAA 3 cut(s) 527, 575, 747
SatI GCNGC 2 cut(s) 594, 1005
Sau3AI GATC 5 cut(s) 59, 256, 676, 861, 1107
Sau96I GGNCC 6 cut(s) 244, 467, 538, 818, 975, 976
ScrFI CCNGG 4 cut(s) 248, 645, 1091, 1127
SduI GDGCHC 4 cut(s) 20, 274, 371, 979
SfaNI GCATC 3 cut(s) 185, 625, 1051
SinI GGWCC 4 cut(s) 244, 467, 538, 818
SmlI CTYRAG 1 cut(s) 455
SmoI CTYRAG 1 cut(s) 455
SnaBI TACGTA 2 cut(s) 185, 707
Sse9I AATT 8 cut(s) 85, 96, 106, 143, 342, 395, 710, 940
SsiI CCGC 6 cut(s) 8, 36, 354, 953, 1005, 1032
SspMI CTAG 6 cut(s) 33, 831, 854, 980, 1016, 1141
StyD4I CCNGG 4 cut(s) 246, 643, 1089, 1125
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 789, 971
TaiI ACGT 3 cut(s) 187, 407, 709
TaqI TCGA 1 cut(s) 58
TasI AATT 8 cut(s) 85, 96, 106, 143, 342, 395, 710, 940
TatI WGTACW 1 cut(s) 931
TauI GCSGC 1 cut(s) 1007
TfiI GAWTC 4 cut(s) 123, 443, 916, 996
Tru1I TTAA 3 cut(s) 527, 575, 747
Tru9I TTAA 3 cut(s) 527, 575, 747
TscAI CASTG 1 cut(s) 794
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 79, 408, 777
TspGWI ACGGA 2 cut(s) 354, 1097
TspRI CASTG 1 cut(s) 794
VpaK11BI GGWCC 4 cut(s) 244, 467, 538, 818
XapI RAATTY 4 cut(s) 106, 342, 395, 940
XceI RCATGY 2 cut(s) 938, 1052
XcmI CCANNNNNNNNNTGG 1 cut(s) 621
XmiI GTMKAC 1 cut(s) 423
XmnI GAANNNNTTC 1 cut(s) 987
XspI CTAG 6 cut(s) 33, 831, 854, 980, 1016, 1141
Zsp2I ATGCAT 1 cut(s) 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.