Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0006510

Physical Location & Seq

Genomic Coordinates Forward (+)

27708468 .. 27715051

Gene Structure 6584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1214300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 867 bp

ATGGCATTAGTTGATGCGAAGATTGCACGAAGAGGAGATTTTTCCAAGCCTTCTGACATCCTGGTAAAATGGAGTCCGGCTGTGGTTGTGGACAGGTCGTTACCTGAGCCTCCAACAGCTGTTTTTCTGCATGGAATTCTAGGTAGCAAGAAGAACTGGGGAACCTTCACCAGGAGATTGGCACAAGAATTTCCGACATGGCAGTTTCTGTTAGTGGACCTGCGGTGTCATGGTGATTCAGCATCAACTAAGAAAAGGGGACCCCATAGTGTTTCATCCACTGCGCTTGATGTCCTAGAACTGGTTCGACAGCTTAGATTAACACCTCGGGTTATAGTCGGACACAGTTTTGGAGGGAAAGTTGCCTTGAGCATGGTGGATCAAGCTGCAAAACCTCTTGCACGACCAGTCAGAGTTTCCTCAAAGCGGGATGTTGTGGATGCTCTAGTTCAAAAAGGATTCTCCAAAGATGTCGCACAGTGGGTGGTTACTAATCTTCGACCAACCAATTCTCTTGGTTCATCACCATCAACCTTCCCATGGGTGTTCGATCTGAAGGGAATCGCTGAAATGTACAAATCCTATGAAGAAACAAATTTATGGCAATTAGTTCATATCCTTATCTTTTGGATTATTTTTATATATGACACAGGAGTCAATATCTACCGGTTAAATATCCTTTTAATCAACCAAAATACTGCCCATTTGTCTGAGCTGGTGTTGGCTCTTATTGGATCGGATCATTGTAGGAAAATTGTTGAAGATGTGCCTCGAGGTGTCCATGTAAACATTTTGAAAGCAGAAAGGAGCTTGCACAGATGGGCTTTACGAGATCTCCACAGAATTCACGCGGCTAAGTGTCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_6

Protein Analysis

289

Amino Acids

32.4

Weight (kDa)

9.89

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Abhydrolase_6	PF12697	41 - 167	8.8e-14	Alpha/beta hydrolase family
Abhydrolase_1	PF00561	41 - 141	4.8e-11	alpha/beta hydrolase fold
Hydrolase_4	PF12146	41 - 172	1.8e-07	Serine aminopeptidase, S33

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006510)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G10030
fragaria_vesca	FvH4_5g33210 FvH4_5g33210 FvH4_5g33210 FvH4_5g33210 FvH4_5g33210
malus_domestica	MD08G1214000.v1.1 MD08G1214300.v1.1 MD15G1397200.v1.1
prunus_persica	Prupe.1G546000_v2.0.a1
pyrus_communis	pycom08g18360 pycom08g18370 pycom08g18410 pycom15g35690
rosa_chinensis	RchiOBHm_Chr7g0231591
rosa_laevigata	RLG00000001408
rosa_multiflora	Rmu_co8305357.1_g000001 Rmu_sc0001377.1_g000024
rosa_roxburghii	Rroxscaffold_3G00229070
rosa_rugosa	Rorug07G0267300
rosa_samantha	Rh7AG422100 Rh7BG396400 Rh7CG442300 Rh7DG414400
rosa_wichuraiana	Rw7G034800 Rw7G034870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	653
AbsI	CCTCGAGG	1 cut(s)	771
Acc36I	ACCTGC	1 cut(s)	228
AccII	CGCG	1 cut(s)	851
AciI	CCGC	3 cut(s)	223, 427, 851
AclWI	GGATC	3 cut(s)	387, 742, 747
AcsI	RAATTY	4 cut(s)	135, 188, 595, 843
AcuI	CTGAAG	1 cut(s)	575
AfaI	GTAC	1 cut(s)	575
AfiI	CCNNNNNNNGG	4 cut(s)	171, 301, 426, 540
AgeI	ACCGGT	1 cut(s)	666
AgsI	TTSAA	3 cut(s)	452, 761, 796
AjnI	CCWGG	2 cut(s)	60, 170
AluBI	AGCT	5 cut(s)	119, 313, 386, 715, 810
AluI	AGCT	5 cut(s)	119, 313, 386, 715, 810
AlwI	GGATC	3 cut(s)	387, 742, 747
AlwNI	CAGNNNCTG	1 cut(s)	208
Ama87I	CYCGRG	2 cut(s)	327, 771
ApeKI	GCWGC	1 cut(s)	386
ApoI	RAATTY	4 cut(s)	135, 188, 595, 843
AsiGI	ACCGGT	1 cut(s)	666
Asp700I	GAANNNNTTC	1 cut(s)	303
AspLEI	GCGC	1 cut(s)	286
AspS9I	GGNCC	2 cut(s)	217, 260
AsuHPI	GGTGA	3 cut(s)	160, 245, 516
AvaI	CYCGRG	2 cut(s)	327, 771
AvaII	GGWCC	2 cut(s)	217, 260
BbvI	GCAGC	1 cut(s)	373
BccI	CCATC	2 cut(s)	535, 813
BciT130I	CCWGG	2 cut(s)	62, 172
BfaI	CTAG	3 cut(s)	140, 296, 446
BfuAI	ACCTGC	1 cut(s)	228
BglII	AGATCT	1 cut(s)	832
BisI	GCNGC	2 cut(s)	387, 852
BlsI	GCNGC	2 cut(s)	388, 853
Bme1390I	CCNGG	2 cut(s)	62, 172
Bme18I	GGWCC	2 cut(s)	217, 260
BmeT110I	CYCGRG	2 cut(s)	327, 771
BmgT120I	GGNCC	2 cut(s)	217, 260
BmiI	GGNNCC	3 cut(s)	163, 261, 262
BmrFI	CCNGG	2 cut(s)	62, 172
BmrI	ACTGGG	1 cut(s)	166
BmsI	GCATC	3 cut(s)	4, 251, 430
BmuI	ACTGGG	1 cut(s)	166
Bpu10I	CCTNAGC	1 cut(s)	105
BpuEI	CTTGAG	1 cut(s)	388
BsaJI	CCNNGG	2 cut(s)	326, 539
BsaWI	WCCGGW	1 cut(s)	666
BsaXI	ACNNNNNCTCC	4 cut(s)	345, 375, 819, 849
Bsc4I	CCNNNNNNNGG	4 cut(s)	171, 301, 426, 540
Bse118I	RCCGGY	1 cut(s)	666
Bse1I	ACTGG	3 cut(s)	161, 306, 407
BseBI	CCWGG	2 cut(s)	62, 172
BseDI	CCNNGG	2 cut(s)	326, 539
BseGI	GGATG	4 cut(s)	57, 275, 436, 445
BseLI	CCNNNNNNNGG	4 cut(s)	171, 301, 426, 540
BseMII	CTCAG	2 cut(s)	96, 702
BseNI	ACTGG	3 cut(s)	161, 306, 407
BseRI	GAGGAG	1 cut(s)	48
BseXI	GCAGC	1 cut(s)	373
Bsh1236I	CGCG	1 cut(s)	851
BshTI	ACCGGT	1 cut(s)	666
BsiHKCI	CYCGRG	2 cut(s)	327, 771
BsiSI	CCGG	2 cut(s)	77, 667
BslFI	GGGAC	1 cut(s)	273
BslI	CCNNNNNNNGG	4 cut(s)	171, 301, 426, 540
BsmFI	GGGAC	1 cut(s)	273
BsoBI	CYCGRG	2 cut(s)	327, 771
Bsp1407I	TGTACA	1 cut(s)	573
Bsp143I	GATC	5 cut(s)	379, 550, 734, 739, 832
Bsp19I	CCATGG	1 cut(s)	539
BspACI	CCGC	3 cut(s)	223, 427, 851
BspCNI	CTCAG	2 cut(s)	97, 703
BspFNI	CGCG	1 cut(s)	851
BspLI	GGNNCC	3 cut(s)	163, 261, 262
BspMI	ACCTGC	1 cut(s)	228
BspPI	GGATC	3 cut(s)	387, 742, 747
BsrFI	RCCGGY	1 cut(s)	666
BsrGI	TGTACA	1 cut(s)	573
BsrI	ACTGG	3 cut(s)	161, 306, 407
BssAI	RCCGGY	1 cut(s)	666
BssECI	CCNNGG	2 cut(s)	326, 539
BssMI	GATC	5 cut(s)	379, 550, 734, 739, 832
BssT1I	CCWWGG	1 cut(s)	539
Bst2UI	CCWGG	2 cut(s)	62, 172
Bst4CI	ACNGT	2 cut(s)	347, 480
Bst6I	CTCTTC	1 cut(s)	25
BstAUI	TGTACA	1 cut(s)	573
BstC8I	GCNNGC	1 cut(s)	812
BstDEI	CTNAG	5 cut(s)	105, 249, 314, 711, 855
BstDSI	CCRYGG	1 cut(s)	539
BstENI	CCTNNNNNAGG	1 cut(s)	169
BstF5I	GGATG	4 cut(s)	57, 275, 436, 445
BstFNI	CGCG	1 cut(s)	851
BstHHI	GCGC	1 cut(s)	286
BstKTI	GATC	5 cut(s)	382, 553, 737, 742, 835
BstMBI	GATC	5 cut(s)	379, 550, 734, 739, 832
BstMWI	GCNNNNNNNGC	1 cut(s)	23
BstNI	CCWGG	2 cut(s)	62, 172
BstSCI	CCNGG	2 cut(s)	60, 170
BstUI	CGCG	1 cut(s)	851
BstV1I	GCAGC	1 cut(s)	373
BstX2I	RGATCY	1 cut(s)	832
BstXI	CCANNNNNNTGG	1 cut(s)	178
BstYI	RGATCY	1 cut(s)	832
BtgI	CCRYGG	1 cut(s)	539
BtsCI	GGATG	4 cut(s)	57, 275, 436, 445
BtsI	GCAGTG	1 cut(s)	279
BtsIMutI	CAGTG	2 cut(s)	279, 485
BveI	ACCTGC	1 cut(s)	228
Cac8I	GCNNGC	1 cut(s)	812
CaiI	CAGNNNCTG	1 cut(s)	208
CfoI	GCGC	1 cut(s)	286
Cfr10I	RCCGGY	1 cut(s)	666
Cfr13I	GGNCC	2 cut(s)	217, 260
Csp6I	GTAC	1 cut(s)	574
CspAI	ACCGGT	1 cut(s)	666
CspCI	CAANNNNNGTGG	2 cut(s)	268, 303
CviAII	CATG	6 cut(s)	131, 198, 230, 373, 540, 782
CviQI	GTAC	1 cut(s)	574
DdeI	CTNAG	5 cut(s)	105, 249, 314, 711, 855
DpnI	GATC	5 cut(s)	381, 552, 736, 741, 834
DpnII	GATC	5 cut(s)	379, 550, 734, 739, 832
DrdI	GACNNNNNNGTC	1 cut(s)	653
DseDI	GACNNNNNNGTC	1 cut(s)	653
Eam1104I	CTCTTC	1 cut(s)	25
EarI	CTCTTC	1 cut(s)	25
Eco130I	CCWWGG	1 cut(s)	539
Eco47I	GGWCC	2 cut(s)	217, 260
Eco57I	CTGAAG	1 cut(s)	575
Eco88I	CYCGRG	2 cut(s)	327, 771
EcoNI	CCTNNNNNAGG	1 cut(s)	169
EcoO109I	RGGNCCY	1 cut(s)	260
EcoRI	GAATTC	2 cut(s)	135, 843
EcoRII	CCWGG	2 cut(s)	60, 170
EcoT14I	CCWWGG	1 cut(s)	539
ErhI	CCWWGG	1 cut(s)	539
FaeI	CATG	6 cut(s)	134, 201, 233, 376, 543, 785
FaqI	GGGAC	1 cut(s)	273
FatI	CATG	6 cut(s)	130, 197, 229, 372, 539, 781
FauI	CCCGC	1 cut(s)	420
Fnu4HI	GCNGC	2 cut(s)	387, 852
FokI	GGATG	4 cut(s)	44, 262, 443, 452
Fsp4HI	GCNGC	2 cut(s)	387, 852
FspBI	CTAG	3 cut(s)	140, 296, 446
GlaI	GCGC	1 cut(s)	285
GluI	GCNGC	2 cut(s)	387, 852
HapII	CCGG	2 cut(s)	77, 667
HhaI	GCGC	1 cut(s)	286
Hin1II	CATG	6 cut(s)	134, 201, 233, 376, 543, 785
Hin6I	GCGC	1 cut(s)	284
HinP1I	GCGC	1 cut(s)	284
HinfI	GANTC	5 cut(s)	73, 236, 459, 561, 654
HpaII	CCGG	2 cut(s)	77, 667
HphI	GGTGA	3 cut(s)	160, 245, 516
Hpy166II	GTNNAC	3 cut(s)	91, 217, 787
Hpy188I	TCNGA	7 cut(s)	55, 195, 341, 413, 555, 712, 739
Hpy8I	GTNNAC	3 cut(s)	91, 217, 787
HpyAV	CCTTC	4 cut(s)	60, 175, 544, 550
HpyCH4III	ACNGT	2 cut(s)	347, 480
HpyCH4V	TGCA	5 cut(s)	26, 130, 389, 401, 814
HpyF10VI	GCNNNNNNNGC	1 cut(s)	23
HpyF3I	CTNAG	5 cut(s)	105, 249, 314, 711, 855
Hsp92II	CATG	6 cut(s)	134, 201, 233, 376, 543, 785
HspAI	GCGC	1 cut(s)	284
KflI	GGGWCCC	1 cut(s)	260
Kzo9I	GATC	5 cut(s)	379, 550, 734, 739, 832
LmnI	GCTCC	1 cut(s)	807
Lsp1109I	GCAGC	1 cut(s)	373
LweI	GCATC	3 cut(s)	4, 251, 430
MaeI	CTAG	3 cut(s)	140, 296, 446
MaeIII	GTNAC	2 cut(s)	99, 487
MalI	GATC	5 cut(s)	381, 552, 736, 741, 834
MboI	GATC	5 cut(s)	379, 550, 734, 739, 832
MboII	GAAGA	6 cut(s)	31, 42, 163, 488, 599, 773
MflI	RGATCY	1 cut(s)	832
MluCI	AATT	7 cut(s)	135, 188, 508, 595, 605, 753, 843
MlyI	GAGTC	2 cut(s)	82, 663
MmeI	TCCRAC	3 cut(s)	137, 218, 319
MnlI	CCTC	8 cut(s)	26, 120, 336, 347, 405, 430, 767, 780
MroXI	GAANNNNTTC	1 cut(s)	303
MseI	TTAA	3 cut(s)	320, 671, 683
MspA1I	CMGCKG	1 cut(s)	119
MspI	CCGG	2 cut(s)	77, 667
MspR9I	CCNGG	2 cut(s)	62, 172
MvaI	CCWGG	2 cut(s)	62, 172
MvnI	CGCG	1 cut(s)	851
MwoI	GCNNNNNNNGC	1 cut(s)	23
NcoI	CCATGG	1 cut(s)	539
NdeII	GATC	5 cut(s)	379, 550, 734, 739, 832
NlaIII	CATG	6 cut(s)	134, 201, 233, 376, 543, 785
NlaIV	GGNNCC	3 cut(s)	163, 261, 262
PaeR7I	CTCGAG	1 cut(s)	771
PdmI	GAANNNNTTC	1 cut(s)	303
PfeI	GAWTC	3 cut(s)	236, 459, 561
PinAI	ACCGGT	1 cut(s)	666
PkrI	GCNGC	2 cut(s)	388, 853
PleI	GAGTC	2 cut(s)	81, 662
PpsI	GAGTC	2 cut(s)	81, 662
PpuMI	RGGWCCY	1 cut(s)	260
Psp5II	RGGWCCY	1 cut(s)	260
Psp6I	CCWGG	2 cut(s)	60, 170
PspGI	CCWGG	2 cut(s)	60, 170
PspN4I	GGNNCC	3 cut(s)	163, 261, 262
PspPI	GGNCC	2 cut(s)	217, 260
PspPPI	RGGWCCY	1 cut(s)	260
PspXI	VCTCGAGB	1 cut(s)	771
PstNI	CAGNNNCTG	1 cut(s)	208
PsuI	RGATCY	1 cut(s)	832
PvuII	CAGCTG	1 cut(s)	119
RsaI	GTAC	1 cut(s)	575
RsaNI	GTAC	1 cut(s)	574
SaqAI	TTAA	3 cut(s)	320, 671, 683
SatI	GCNGC	2 cut(s)	387, 852
Sau3AI	GATC	5 cut(s)	379, 550, 734, 739, 832
Sau96I	GGNCC	2 cut(s)	217, 260
SchI	GAGTC	2 cut(s)	82, 663
ScrFI	CCNGG	2 cut(s)	62, 172
SfaNI	GCATC	3 cut(s)	4, 251, 430
Sfr274I	CTCGAG	1 cut(s)	771
SinI	GGWCC	2 cut(s)	217, 260
SlaI	CTCGAG	1 cut(s)	771
SmlI	CTYRAG	2 cut(s)	367, 771
SmoI	CTYRAG	2 cut(s)	367, 771
Sse9I	AATT	7 cut(s)	135, 188, 508, 595, 605, 753, 843
SsiI	CCGC	3 cut(s)	223, 427, 851
SspMI	CTAG	3 cut(s)	140, 296, 446
StyD4I	CCNGG	2 cut(s)	60, 170
StyI	CCWWGG	1 cut(s)	539
TaaI	ACNGT	2 cut(s)	347, 480
TaqI	TCGA	4 cut(s)	307, 499, 549, 772
TasI	AATT	7 cut(s)	135, 188, 508, 595, 605, 753, 843
TatI	WGTACW	1 cut(s)	573
TauI	GCSGC	1 cut(s)	854
TfiI	GAWTC	3 cut(s)	236, 459, 561
Tru1I	TTAA	3 cut(s)	320, 671, 683
Tru9I	TTAA	3 cut(s)	320, 671, 683
TscAI	CASTG	2 cut(s)	286, 485
TseI	GCWGC	1 cut(s)	386
TspDTI	ATGAA	4 cut(s)	264, 510, 600, 602
TspRI	CASTG	2 cut(s)	286, 485
VpaK11BI	GGWCC	2 cut(s)	217, 260
XagI	CCTNNNNNAGG	1 cut(s)	169
XapI	RAATTY	4 cut(s)	135, 188, 595, 843
XhoI	CTCGAG	1 cut(s)	771
XmnI	GAANNNNTTC	1 cut(s)	303
XspI	CTAG	3 cut(s)	140, 296, 446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.