AT4G21110

Protein BUD31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003415
Physical Location & Seq
Forward (+)
11267069 .. 11269019
1951 bp
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UTR
Exon/CDS
Intron
AT4G21110.2

Sequence Viewer

Length: 438 bp
ATGCCAAAGGTTAAAACTAACCGAGTCAAGTACCCAGAAGGATGGGAGTTGATCGAGCCTACTCTTCGAGAGCTTGATGCCAAGATGAGAGAAGCTGAGACTGATTCACATGATGGTAAGAGAAAGTGTGAAACTTTATGGCCAATCTTCAAAGTTTCTCATCAGAGGAGTCGGTATGTATATGACCTTTATTACCGAAGGGAAGAAATATCTAAAGAGCTCTATGAATTCTGCTTGGACCAGGGCTATGCAGATCGCAGTCTGATTGCCAAATGGAAAAAGTCGGGATACGAACGTTTATGCTGCTTGCGCTGCATACAGCCAAGAGACCACAACTATGGAACAACATGTGTATGCCGTGTTCCCAAACACTTGCGTGAAGAGAAAGTGGTCGAATGCGTTCACTGCGGCTGTCAAGGATGCGCGAGTGGCGATTAA

Protein Analysis

145

Amino Acids

17.19

Weight (kDa)

8.45

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BUD31 PF01125 1 - 143 1.4e-66 Pre-mRNA-splicing factor BUD31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 425
AciI CCGC 1 cut(s) 408
AclI AACGTT 1 cut(s) 295
AcoI YGGCCR 1 cut(s) 140
AcsI RAATTY 1 cut(s) 227
AfaI GTAC 1 cut(s) 32
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 1 cut(s) 240
AleI CACNNNNGTG 1 cut(s) 375
AluBI AGCT 3 cut(s) 73, 95, 220
AluI AGCT 3 cut(s) 73, 95, 220
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 92, 321
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 2 cut(s) 303, 312
ApoI RAATTY 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 399
AspLEI GCGC 2 cut(s) 312, 425
AspS9I GGNCC 1 cut(s) 238
AvaII GGWCC 1 cut(s) 238
BaeI ACNNNNGTAYC 2 cut(s) 280, 313
BalI TGGCCA 1 cut(s) 142
BanII GRGCYC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 2 cut(s) 290, 299
BccI CCATC 2 cut(s) 36, 107
BceAI ACGGC 1 cut(s) 342
BciT130I CCWGG 1 cut(s) 242
BciVI GTATCC 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 92, 321
BfuI GTATCC 1 cut(s) 281
BisI GCNGC 3 cut(s) 304, 313, 409
BlsI GCNGC 3 cut(s) 305, 314, 410
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 67, 410
BsaI GGTCTC 1 cut(s) 321
BsaJI CCNNGG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 241
BseGI GGATG 2 cut(s) 47, 425
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 181
BseXI GCAGC 2 cut(s) 290, 299
Bsh1236I CGCG 1 cut(s) 425
BshFI GGCC 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 222
BsmAI GTCTC 2 cut(s) 92, 321
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 1 cut(s) 142
Bso31I GGTCTC 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 2 cut(s) 51, 253
BspACI CCGC 1 cut(s) 408
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 321
BssECI CCNNGG 1 cut(s) 241
BssMI GATC 2 cut(s) 51, 253
Bst2UI CCWGG 1 cut(s) 242
Bst6I CTCTTC 2 cut(s) 69, 375
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 96
BstF5I GGATG 2 cut(s) 47, 425
BstFNI CGCG 1 cut(s) 425
BstHHI GCGC 2 cut(s) 312, 425
BstKTI GATC 2 cut(s) 54, 256
BstMAI GTCTC 2 cut(s) 92, 321
BstMBI GATC 2 cut(s) 51, 253
BstMWI GCNNNNNNNGC 4 cut(s) 309, 312, 405, 429
BstNI CCWGG 1 cut(s) 242
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 240
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 2 cut(s) 290, 299
BstXI CCANNNNNNTGG 2 cut(s) 42, 338
BsuI GTATCC 1 cut(s) 281
BsuRI GGCC 1 cut(s) 142
BtsCI GGATG 2 cut(s) 47, 425
BtsI GCAGTG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 403
Cac8I GCNNGC 1 cut(s) 308
CfoI GCGC 2 cut(s) 312, 425
Cfr13I GGNCC 1 cut(s) 238
Csp6I GTAC 1 cut(s) 31
CviAII CATG 2 cut(s) 110, 348
CviJI RGCY 8 cut(s) 58, 73, 95, 142, 220, 246, 322, 411
CviKI_1 RGCY 8 cut(s) 58, 73, 95, 142, 220, 246, 322, 411
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 96
DpnI GATC 2 cut(s) 53, 255
DpnII GATC 2 cut(s) 51, 253
EaeI YGGCCR 1 cut(s) 140
Eam1104I CTCTTC 2 cut(s) 69, 375
EarI CTCTTC 2 cut(s) 69, 375
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 1 cut(s) 222
Eco31I GGTCTC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 222
FaeI CATG 2 cut(s) 113, 351
FatI CATG 2 cut(s) 109, 347
Fnu4HI GCNGC 3 cut(s) 304, 313, 409
FokI GGATG 2 cut(s) 54, 432
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 3 cut(s) 304, 313, 409
GlaI GCGC 2 cut(s) 311, 424
GluI GCNGC 3 cut(s) 304, 313, 409
HaeIII GGCC 1 cut(s) 142
HhaI GCGC 2 cut(s) 312, 425
Hin1II CATG 2 cut(s) 113, 351
Hin6I GCGC 2 cut(s) 310, 423
HinP1I GCGC 2 cut(s) 310, 423
HinfI GANTC 3 cut(s) 24, 104, 169
Hpy166II GTNNAC 1 cut(s) 403
Hpy188I TCNGA 2 cut(s) 165, 264
Hpy188III TCNNGA 2 cut(s) 68, 285
Hpy8I GTNNAC 1 cut(s) 403
HpyAV CCTTC 2 cut(s) 32, 192
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 2 cut(s) 251, 315
HpyF10VI GCNNNNNNNGC 4 cut(s) 309, 312, 405, 429
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 2 cut(s) 113, 351
HspAI GCGC 2 cut(s) 310, 423
Kzo9I GATC 2 cut(s) 51, 253
LpnPI CCDG 3 cut(s) 48, 227, 254
Lsp1109I GCAGC 2 cut(s) 290, 299
LweI GCATC 2 cut(s) 67, 410
MaeII ACGT 1 cut(s) 295
MalI GATC 2 cut(s) 53, 255
MboI GATC 2 cut(s) 51, 253
MboII GAAGA 4 cut(s) 56, 139, 215, 392
MhlI GDGCHC 1 cut(s) 222
MlsI TGGCCA 1 cut(s) 142
MluCI AATT 1 cut(s) 227
MluNI TGGCCA 1 cut(s) 142
MlyI GAGTC 2 cut(s) 33, 178
MnlI CCTC 1 cut(s) 159
Mox20I TGGCCA 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 399
MscI TGGCCA 1 cut(s) 142
MseI TTAA 2 cut(s) 12, 436
MslI CAYNNNNRTG 3 cut(s) 336, 352, 375
Msp20I TGGCCA 1 cut(s) 142
MspR9I CCNGG 1 cut(s) 242
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 1 cut(s) 242
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 4 cut(s) 309, 312, 405, 429
NdeII GATC 2 cut(s) 51, 253
NlaIII CATG 2 cut(s) 113, 351
NspI RCATGY 1 cut(s) 351
OliI CACNNNNGTG 1 cut(s) 375
PciI ACATGT 1 cut(s) 347
PctI GAATGC 1 cut(s) 401
PdmI GAANNNNTTC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 3 cut(s) 305, 314, 410
PleI GAGTC 2 cut(s) 32, 177
PpsI GAGTC 2 cut(s) 32, 177
PscI ACATGT 1 cut(s) 347
Psp124BI GAGCTC 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspPI GGNCC 1 cut(s) 238
PsrI GAACNNNNNNTAC 2 cut(s) 345, 377
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 3 cut(s) 336, 352, 375
SacI GAGCTC 1 cut(s) 222
SaqAI TTAA 2 cut(s) 12, 436
SatI GCNGC 3 cut(s) 304, 313, 409
Sau3AI GATC 2 cut(s) 51, 253
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 2 cut(s) 33, 178
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 1 cut(s) 222
SetI ASST 6 cut(s) 12, 75, 97, 189, 222, 298
SfaNI GCATC 2 cut(s) 67, 410
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 3 cut(s) 336, 352, 375
Sse9I AATT 1 cut(s) 227
SsiI CCGC 1 cut(s) 408
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 240
TaiI ACGT 1 cut(s) 298
TaqI TCGA 3 cut(s) 54, 67, 393
TasI AATT 1 cut(s) 227
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 2 cut(s) 12, 436
Tru9I TTAA 2 cut(s) 12, 436
TscAI CASTG 1 cut(s) 410
TseI GCWGC 2 cut(s) 303, 312
TspDTI ATGAA 1 cut(s) 240
TspRI CASTG 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 238
XapI RAATTY 1 cut(s) 227
XceI RCATGY 1 cut(s) 351
XmnI GAANNNNTTC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.