RLG00000014017

Protein BUD31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003415
Physical Location & Seq
Forward (+)
45781917 .. 45782981
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014017

Sequence Viewer

Length: 354 bp
ATGAGAGAAGCTGAGAATGATACTCATGATGGTAAGAGAAAATGTGAAACGTTATGGCCTATTTTCAAAATAGCACATCAAAAGAGCCGCTACATTTTTGACCTTTACCATCGGAGAAAAGAAATTTCCAAGGAATTGTATGAGTTCTGCCTGGACCAAGGTTATGCTGACCGCAATCTAATTGCTAAGTGGAAGAAGCCTGGTTATGAACACCTCTGCTGTTTGAGGTGCATGCAGCCTCGGGATCACAACTTTGCCACCACATGCGTCTGCCGAGTGCCCAAGCATCTGAGGGAGGAGAAGGTTATAGAGTGTGTGCATTGTGGCTGCAGGGGCTGTGCCAGTGGAGATTGA

Protein Analysis

118

Amino Acids

13.91

Weight (kDa)

8.64

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BUD31 PF01125 1 - 115 3.9e-55 Pre-mRNA-splicing factor BUD31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 88, 172
AclI AACGTT 1 cut(s) 50
AclWI GGATC 1 cut(s) 252
AcsI RAATTY 1 cut(s) 123
AgsI TTSAA 1 cut(s) 67
AjnI CCWGG 2 cut(s) 150, 199
AluBI AGCT 1 cut(s) 11
AluI AGCT 1 cut(s) 11
AlwI GGATC 1 cut(s) 252
AlwNI CAGNNNCTG 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 1 cut(s) 56
ApeKI GCWGC 2 cut(s) 235, 327
ApoI RAATTY 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 282
BbvI GCAGC 2 cut(s) 247, 314
BccI CCATC 2 cut(s) 23, 117
BciT130I CCWGG 2 cut(s) 152, 201
BfmI CTRYAG 1 cut(s) 328
BisI GCNGC 3 cut(s) 88, 236, 328
BlsI GCNGC 3 cut(s) 89, 237, 329
Bme1390I CCNGG 2 cut(s) 152, 201
Bme18I GGWCC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 154
BmrFI CCNGG 2 cut(s) 152, 201
BmsI GCATC 1 cut(s) 295
BsaJI CCNNGG 3 cut(s) 129, 157, 239
Bse1I ACTGG 1 cut(s) 342
BseBI CCWGG 2 cut(s) 152, 201
BseDI CCNNGG 3 cut(s) 129, 157, 239
BseMII CTCAG 1 cut(s) 281
BseNI ACTGG 1 cut(s) 342
BseRI GAGGAG 1 cut(s) 311
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 2 cut(s) 247, 314
BshFI GGCC 1 cut(s) 58
BsiHKCI CYCGRG 1 cut(s) 240
BsnI GGCC 1 cut(s) 58
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 2 cut(s) 88, 172
BspANI GGCC 1 cut(s) 58
BspCNI CTCAG 2 cut(s) 4, 282
BspHI TCATGA 1 cut(s) 25
BspMAI CTGCAG 1 cut(s) 332
BspPI GGATC 1 cut(s) 252
BsrI ACTGG 1 cut(s) 342
BssECI CCNNGG 3 cut(s) 129, 157, 239
BssMI GATC 1 cut(s) 244
BssT1I CCWWGG 2 cut(s) 129, 157
Bst2UI CCWGG 2 cut(s) 152, 201
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 3 cut(s) 12, 186, 290
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 333
BstNI CCWGG 2 cut(s) 152, 201
BstNSI RCATGY 2 cut(s) 235, 267
BstSCI CCNGG 2 cut(s) 150, 199
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 282
BstV1I GCAGC 2 cut(s) 247, 314
BsuRI GGCC 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 233
CaiI CAGNNNCTG 1 cut(s) 336
CciI TCATGA 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 154
CseI GACGC 1 cut(s) 256
CviAII CATG 3 cut(s) 26, 232, 264
CviJI RGCY 7 cut(s) 11, 58, 87, 199, 238, 327, 336
CviKI_1 RGCY 7 cut(s) 11, 58, 87, 199, 238, 327, 336
DdeI CTNAG 3 cut(s) 12, 186, 290
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eco130I CCWWGG 2 cut(s) 129, 157
Eco47I GGWCC 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 150, 199
EcoT14I CCWWGG 2 cut(s) 129, 157
ErhI CCWWGG 2 cut(s) 129, 157
FaeI CATG 3 cut(s) 29, 235, 267
FaiI YATR 8 cut(s) 27, 55, 141, 165, 207, 233, 265, 308
FatI CATG 3 cut(s) 25, 231, 263
Fnu4HI GCNGC 3 cut(s) 88, 236, 328
Fsp4HI GCNGC 3 cut(s) 88, 236, 328
GluI GCNGC 3 cut(s) 88, 236, 328
HaeIII GGCC 1 cut(s) 58
HgaI GACGC 1 cut(s) 256
Hin1II CATG 3 cut(s) 29, 235, 267
Hpy188I TCNGA 2 cut(s) 114, 291
Hpy188III TCNNGA 2 cut(s) 26, 242
HpyAV CCTTC 1 cut(s) 295
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 4 cut(s) 231, 235, 319, 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 333
HpyF3I CTNAG 3 cut(s) 12, 186, 290
HpySE526I ACGT 1 cut(s) 50
Hsp92II CATG 3 cut(s) 29, 235, 267
Kzo9I GATC 1 cut(s) 244
LpnPI CCDG 5 cut(s) 137, 164, 186, 213, 316
Lsp1109I GCAGC 2 cut(s) 247, 314
LweI GCATC 1 cut(s) 295
MaeII ACGT 1 cut(s) 50
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 1 cut(s) 205
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 3 cut(s) 123, 134, 180
MnlI CCTC 5 cut(s) 219, 224, 249, 285, 289
MspR9I CCNGG 2 cut(s) 152, 201
MvaI CCWGG 2 cut(s) 152, 201
MwoI GCNNNNNNNGC 1 cut(s) 333
NdeII GATC 1 cut(s) 244
NlaIII CATG 3 cut(s) 29, 235, 267
NmeAIII GCCGAG 1 cut(s) 299
NspI RCATGY 2 cut(s) 235, 267
PaeI GCATGC 1 cut(s) 235
PagI TCATGA 1 cut(s) 25
PkrI GCNGC 3 cut(s) 89, 237, 329
Psp1406I AACGTT 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 150, 199
PspGI CCWGG 2 cut(s) 150, 199
PspPI GGNCC 1 cut(s) 154
PstI CTGCAG 1 cut(s) 332
PstNI CAGNNNCTG 1 cut(s) 336
SatI GCNGC 3 cut(s) 88, 236, 328
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 154
ScrFI CCNGG 2 cut(s) 152, 201
SduI GDGCHC 1 cut(s) 282
SetI ASST 7 cut(s) 13, 53, 105, 163, 216, 230, 306
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 328
SinI GGWCC 1 cut(s) 154
SphI GCATGC 1 cut(s) 235
Sse9I AATT 3 cut(s) 123, 134, 180
SsiI CCGC 2 cut(s) 88, 172
StyD4I CCNGG 2 cut(s) 150, 199
StyI CCWWGG 2 cut(s) 129, 157
TaiI ACGT 1 cut(s) 53
TasI AATT 3 cut(s) 123, 134, 180
TauI GCSGC 1 cut(s) 90
TscAI CASTG 1 cut(s) 349
TseI GCWGC 2 cut(s) 235, 327
TspDTI ATGAA 1 cut(s) 222
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 154
XapI RAATTY 1 cut(s) 123
XceI RCATGY 2 cut(s) 235, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.