Rorug06G0031200

Protein BUD31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003415
Physical Location & Seq
Reverse (-)
3743794 .. 3745999
2206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0031200.1

Sequence Viewer

Length: 1167 bp
ATGACGACCTCTCTGAAAAGCAGTTTCATGCCTCCGAGCTTGATCTCGAATCTTCAACAAGTTTTGGCTCTCAAAAGCGACGGTGCAGAGAAGCATGCTTCTGAACCAAACAAAGTTTCTTGTAATTCTTCTTCCTCTTCATGTTGCGCTTTGATGCCTTCTGAGGACACTAAAGACAAGCAGTGTCCAAAGCCAGTGGTTTTAGTCACAAATGGAGAAGGAATCGAGTCTCCTGGCCTCACATTTCTCGTCCAAGCACTCCTTCTCGATTCTCATCTTGAGGTCTGCGTCTGCGCTCCTAAATCAGATAGATCAGTGGCTGGCCACTCTGTAACTGTTGGTGAGACCATTTCAGTGTCTTCTGCACAAATCAATGGCGCCACGGCTTTTGAAGTTTCCGGGACTCCTGCGGATTGTATCTCTTTAGCATTATCAGGGGCATTGTTCTCCAACTTTTCAAAGCCTGCTTTGGTAGTCAGTGGAATAAACAGAGGATCAAGTTCTGGAATCAACATGTTCTACTCGGGTGCTGTTGCTGGAGCTAGAGAGGCGTTGCTATGCGGTGTACCCTCTCTGAAGAAGGATGTAAGCTGTGAAAGTGATATGAAGGATGCAGCCACTGTGTGCTCACCGTTGATATGTGCAGCTGCAAAAAGCATCCAGGAGGGAGTTTTCCCCAAAAGTTGTTTACTCAATATTGAGATTCCTAGTTGTCCTTTGACAAATAAGGGCTTCAAAGTGACCAAGCAGAGTCAGTGGAGGTCCTCTTTGAGCTGGAAAGCTGTGTCAACAAACAAACATCCTCCCCCTGCAAATTTCATGTCCAATCAGCAAAGCCTTGGTATTCAACTAGCACAGCTCAGCCGCGAAGCTTCAATAGCTGGTGCAGCTCGCCGCTTGAACTCTCAGCGCAAGACTGTAGAGGTTGAATCGGTTGGTGTAGCCAGAAAAATGAGCTCCCAAAAAACAGTAAAATTCTTCCGTTTAGAGTTCTTAGAGGAGCAGCAGGAATATGTAGATGAAGATCTAGATATCAGAGCACTTGAAGATGGATTTGTTGCGATTACTCCGATTTGCTTATCTCCATCTGTTCAATCGGAAATTCAAACGTCAGTGTCGAATTGGATTGCTGGAGCAGTACTTTCGAACAACCGGTTGGCATTATAG

Protein Analysis

388

Amino Acids

41.05

Weight (kDa)

6.88

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SurE PF01975 67 - 255 1.3e-46 Survival protein SurE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 377
AccII CGCG 1 cut(s) 867
AciI CCGC 4 cut(s) 410, 561, 865, 895
AclWI GGATC 1 cut(s) 502
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 3 cut(s) 814, 974, 1101
AcuI CTGAAG 1 cut(s) 596
AcyI GRCGYC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 624
AfaI GTAC 2 cut(s) 567, 1140
AflIII ACRYGT 1 cut(s) 513
AgeI ACCGGT 1 cut(s) 1152
AjnI CCWGG 2 cut(s) 232, 660
AjuI GAANNNNNNNTTGG 5 cut(s) 100, 132, 246, 278, 1139
Alw21I GWGCWC 3 cut(s) 629, 959, 1042
Alw26I GTCTC 2 cut(s) 234, 338
AlwI GGATC 1 cut(s) 502
AlwNI CAGNNNCTG 2 cut(s) 320, 620
Ama87I CYCGRG 1 cut(s) 523
AoxI GGCC 2 cut(s) 235, 322
ApeKI GCWGC 5 cut(s) 614, 644, 647, 887, 1003
ApoI RAATTY 3 cut(s) 814, 974, 1101
ArsI GACNNNNNNTTYG 2 cut(s) 806, 838
AsiGI ACCGGT 1 cut(s) 1152
AspLEI GCGC 4 cut(s) 149, 296, 380, 912
AspS9I GGNCC 1 cut(s) 762
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 2 cut(s) 353, 621
AsuII TTCGAA 1 cut(s) 1145
AvaI CYCGRG 1 cut(s) 523
AvaII GGWCC 1 cut(s) 762
BalI TGGCCA 1 cut(s) 324
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 959
BarI GAAGNNNNNNTAC 2 cut(s) 115, 147
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 3 cut(s) 629, 959, 1042
BbvI GCAGC 5 cut(s) 626, 634, 656, 899, 1015
BccI CCATC 2 cut(s) 1043, 1093
BceAI ACGGC 1 cut(s) 399
BcgI CGANNNNNNTGC 2 cut(s) 1125, 1159
BciT130I CCWGG 2 cut(s) 234, 662
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 2 cut(s) 234, 338
BfaI CTAG 4 cut(s) 543, 708, 851, 1028
BfmI CTRYAG 1 cut(s) 918
BfoI RGCGCY 1 cut(s) 381
BglII AGATCT 1 cut(s) 1024
BisI GCNGC 7 cut(s) 615, 645, 648, 865, 888, 895, 1004
BlpI GCTNAGC 1 cut(s) 860
BlsI GCNGC 7 cut(s) 616, 646, 649, 866, 889, 896, 1005
BmcAI AGTACT 1 cut(s) 1140
Bme1390I CCNGG 3 cut(s) 234, 400, 662
Bme18I GGWCC 1 cut(s) 762
BmeT110I CYCGRG 1 cut(s) 523
BmgT120I GGNCC 1 cut(s) 762
BmiI GGNNCC 1 cut(s) 379
BmrFI CCNGG 3 cut(s) 234, 400, 662
BmsI GCATC 3 cut(s) 144, 601, 666
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 2 cut(s) 558, 1152
Bpu1102I GCTNAGC 1 cut(s) 860
Bpu14I TTCGAA 1 cut(s) 1145
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 400
BsaBI GATNNNNATC 2 cut(s) 273, 1023
BsaHI GRCGYC 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 381, 838
BsaWI WCCGGW 1 cut(s) 1152
Bse118I RCCGGY 1 cut(s) 1152
Bse1I ACTGG 1 cut(s) 194
Bse8I GATNNNNATC 2 cut(s) 273, 1023
BseBI CCWGG 2 cut(s) 234, 662
BseDI CCNNGG 2 cut(s) 381, 838
BseGI GGATG 4 cut(s) 589, 616, 657, 799
BseJI GATNNNNATC 2 cut(s) 273, 1023
BseMII CTCAG 3 cut(s) 153, 874, 920
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 1013
BseXI GCAGC 5 cut(s) 626, 634, 656, 899, 1015
BsgI GTGCAG 4 cut(s) 105, 348, 663, 906
Bsh1236I CGCG 1 cut(s) 867
BshFI GGCC 2 cut(s) 237, 324
BshNI GGYRCC 1 cut(s) 377
BshTI ACCGGT 1 cut(s) 1152
BsiHKAI GWGCWC 3 cut(s) 629, 959, 1042
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 2 cut(s) 399, 1153
BslFI GGGAC 1 cut(s) 415
BsmAI GTCTC 2 cut(s) 234, 338
BsmFI GGGAC 1 cut(s) 415
BsnI GGCC 2 cut(s) 237, 324
Bso31I GGTCTC 1 cut(s) 338
BsoBI CYCGRG 1 cut(s) 523
Bsp119I TTCGAA 1 cut(s) 1145
Bsp1286I GDGCHC 3 cut(s) 629, 959, 1042
Bsp143I GATC 4 cut(s) 42, 311, 494, 1024
Bsp1720I GCTNAGC 1 cut(s) 860
BspACI CCGC 4 cut(s) 410, 561, 865, 895
BspANI GGCC 2 cut(s) 237, 324
BspCNI CTCAG 3 cut(s) 154, 873, 919
BspFNI CGCG 1 cut(s) 867
BspLI GGNNCC 1 cut(s) 379
BspPI GGATC 1 cut(s) 502
BspT104I TTCGAA 1 cut(s) 1145
BspT107I GGYRCC 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 338
BsrFI RCCGGY 1 cut(s) 1152
BsrI ACTGG 1 cut(s) 194
BssAI RCCGGY 1 cut(s) 1152
BssECI CCNNGG 2 cut(s) 381, 838
BssMI GATC 4 cut(s) 42, 311, 494, 1024
BssNI GRCGYC 1 cut(s) 378
BssT1I CCWWGG 1 cut(s) 838
Bst2UI CCWGG 2 cut(s) 234, 662
Bst4CI ACNGT 6 cut(s) 83, 337, 622, 633, 919, 970
Bst6I CTCTTC 1 cut(s) 142
BstACI GRCGYC 1 cut(s) 378
BstBI TTCGAA 1 cut(s) 1145
BstC8I GCNNGC 4 cut(s) 96, 322, 465, 892
BstDEI CTNAG 4 cut(s) 162, 860, 906, 994
BstDSI CCRYGG 1 cut(s) 381
BstF5I GGATG 4 cut(s) 589, 616, 657, 799
BstFNI CGCG 1 cut(s) 867
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 4 cut(s) 149, 296, 380, 912
BstKTI GATC 4 cut(s) 45, 314, 497, 1027
BstMAI GTCTC 2 cut(s) 234, 338
BstMBI GATC 4 cut(s) 42, 311, 494, 1024
BstMWI GCNNNNNNNGC 3 cut(s) 548, 878, 887
BstNI CCWGG 2 cut(s) 234, 662
BstNSI RCATGY 2 cut(s) 98, 517
BstSCI CCNGG 3 cut(s) 232, 398, 660
BstSFI CTRYAG 1 cut(s) 918
BstUI CGCG 1 cut(s) 867
BstV1I GCAGC 5 cut(s) 626, 634, 656, 899, 1015
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 1024
BstYI RGATCY 1 cut(s) 1024
BsuRI GGCC 2 cut(s) 237, 324
BtgI CCRYGG 1 cut(s) 381
BtsCI GGATG 4 cut(s) 589, 616, 657, 799
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 8 cut(s) 188, 201, 321, 360, 484, 618, 761, 1119
Cac8I GCNNGC 4 cut(s) 96, 322, 465, 892
CaiI CAGNNNCTG 2 cut(s) 320, 620
CfoI GCGC 4 cut(s) 149, 296, 380, 912
Cfr10I RCCGGY 1 cut(s) 1152
Cfr13I GGNCC 1 cut(s) 762
CseI GACGC 1 cut(s) 277
Csp6I GTAC 2 cut(s) 566, 1139
CspAI ACCGGT 1 cut(s) 1152
CspCI CAANNNNNGTGG 4 cut(s) 177, 212, 370, 405
CviAII CATG 5 cut(s) 28, 95, 141, 514, 820
CviQI GTAC 2 cut(s) 566, 1139
DdeI CTNAG 4 cut(s) 162, 860, 906, 994
DinI GGCGCC 1 cut(s) 379
DpnI GATC 4 cut(s) 44, 313, 496, 1026
DpnII GATC 4 cut(s) 42, 311, 494, 1024
DraIII CACNNNGTG 1 cut(s) 624
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 142
EarI CTCTTC 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 957
Eco130I CCWWGG 1 cut(s) 838
Eco24I GRGCYC 1 cut(s) 959
Eco31I GGTCTC 1 cut(s) 338
Eco32I GATATC 1 cut(s) 1033
Eco47I GGWCC 1 cut(s) 762
Eco53kI GAGCTC 1 cut(s) 957
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 523
EcoICRI GAGCTC 1 cut(s) 957
EcoO109I RGGNCCY 1 cut(s) 762
EcoRII CCWGG 2 cut(s) 232, 660
EcoRV GATATC 1 cut(s) 1033
EcoT14I CCWWGG 1 cut(s) 838
EcoT38I GRGCYC 1 cut(s) 959
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 838
FaeI CATG 5 cut(s) 31, 98, 144, 517, 823
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 1 cut(s) 415
FatI CATG 5 cut(s) 27, 94, 140, 513, 819
Fnu4HI GCNGC 7 cut(s) 615, 645, 648, 865, 888, 895, 1004
FokI GGATG 4 cut(s) 596, 623, 644, 786
FriOI GRGCYC 1 cut(s) 959
Fsp4HI GCNGC 7 cut(s) 615, 645, 648, 865, 888, 895, 1004
FspBI CTAG 4 cut(s) 543, 708, 851, 1028
GlaI GCGC 4 cut(s) 148, 295, 379, 911
GluI GCNGC 7 cut(s) 615, 645, 648, 865, 888, 895, 1004
GsuI CTGGAG 2 cut(s) 558, 1152
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 2 cut(s) 237, 324
HapII CCGG 2 cut(s) 399, 1153
HgaI GACGC 1 cut(s) 277
HhaI GCGC 4 cut(s) 149, 296, 380, 912
Hin1I GRCGYC 1 cut(s) 378
Hin1II CATG 5 cut(s) 31, 98, 144, 517, 823
Hin6I GCGC 4 cut(s) 147, 294, 378, 910
HinP1I GCGC 4 cut(s) 147, 294, 378, 910
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 1 cut(s) 870
HinfI GANTC 9 cut(s) 49, 222, 227, 269, 403, 507, 703, 751, 929
HpaII CCGG 2 cut(s) 399, 1153
HphI GGTGA 2 cut(s) 353, 621
Hpy166II GTNNAC 3 cut(s) 566, 689, 789
Hpy188I TCNGA 9 cut(s) 15, 36, 103, 163, 307, 576, 1037, 1071, 1099
Hpy188III TCNNGA 5 cut(s) 46, 266, 278, 504, 1028
Hpy8I GTNNAC 3 cut(s) 566, 689, 789
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 5 cut(s) 168, 212, 272, 574, 601
HpyCH4III ACNGT 6 cut(s) 83, 337, 622, 633, 919, 970
HpyCH4IV ACGT 1 cut(s) 1109
HpyCH4V TGCA 7 cut(s) 86, 365, 614, 644, 650, 812, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 548, 878, 887
HpyF3I CTNAG 4 cut(s) 162, 860, 906, 994
HpySE526I ACGT 1 cut(s) 1109
Hsp92I GRCGYC 1 cut(s) 378
Hsp92II CATG 5 cut(s) 31, 98, 144, 517, 823
HspAI GCGC 4 cut(s) 147, 294, 378, 910
KasI GGCGCC 1 cut(s) 377
Kzo9I GATC 4 cut(s) 42, 311, 494, 1024
LmnI GCTCC 5 cut(s) 301, 539, 962, 1000, 1133
Lsp1109I GCAGC 5 cut(s) 626, 634, 656, 899, 1015
LweI GCATC 3 cut(s) 144, 601, 666
MaeI CTAG 4 cut(s) 543, 708, 851, 1028
MaeII ACGT 1 cut(s) 1109
MaeIII GTNAC 3 cut(s) 205, 331, 739
MalI GATC 4 cut(s) 44, 313, 496, 1026
MboI GATC 4 cut(s) 42, 311, 494, 1024
MboII GAAGA 9 cut(s) 44, 120, 123, 129, 351, 589, 970, 1034, 1058
MflI RGATCY 1 cut(s) 1024
MhlI GDGCHC 3 cut(s) 629, 959, 1042
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 5 cut(s) 124, 814, 974, 1101, 1120
MluNI TGGCCA 1 cut(s) 324
Mly113I GGCGCC 1 cut(s) 378
MlyI GAGTC 3 cut(s) 236, 397, 760
MmeI TCCRAC 1 cut(s) 474
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MslI CAYNNNNRTG 1 cut(s) 353
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 2 cut(s) 399, 1153
MspR9I CCNGG 3 cut(s) 234, 400, 662
MvaI CCWGG 2 cut(s) 234, 662
MvnI CGCG 1 cut(s) 867
MwoI GCNNNNNNNGC 3 cut(s) 548, 878, 887
NarI GGCGCC 1 cut(s) 378
NciI CCSGG 1 cut(s) 400
NdeII GATC 4 cut(s) 42, 311, 494, 1024
NlaIII CATG 5 cut(s) 31, 98, 144, 517, 823
NlaIV GGNNCC 1 cut(s) 379
NmuCI GTSAC 2 cut(s) 205, 739
NspI RCATGY 2 cut(s) 98, 517
NspV TTCGAA 1 cut(s) 1145
PaeI GCATGC 1 cut(s) 98
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 1115
PfeI GAWTC 6 cut(s) 49, 222, 269, 507, 703, 929
PfoI TCCNGGA 2 cut(s) 398, 660
PinAI ACCGGT 1 cut(s) 1152
PkrI GCNGC 7 cut(s) 616, 646, 649, 866, 889, 896, 1005
PleI GAGTC 3 cut(s) 235, 397, 759
PluTI GGCGCC 1 cut(s) 381
PpsI GAGTC 3 cut(s) 235, 397, 759
PpuMI RGGWCCY 1 cut(s) 762
PscI ACATGT 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 959
Psp5II RGGWCCY 1 cut(s) 762
Psp6I CCWGG 2 cut(s) 232, 660
PspGI CCWGG 2 cut(s) 232, 660
PspN4I GGNNCC 1 cut(s) 379
PspPI GGNCC 1 cut(s) 762
PspPPI RGGWCCY 1 cut(s) 762
PstNI CAGNNNCTG 2 cut(s) 320, 620
PsuI RGATCY 1 cut(s) 1024
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 2 cut(s) 567, 1140
RsaNI GTAC 2 cut(s) 566, 1139
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 959
SatI GCNGC 7 cut(s) 615, 645, 648, 865, 888, 895, 1004
Sau3AI GATC 4 cut(s) 42, 311, 494, 1024
Sau96I GGNCC 1 cut(s) 762
ScaI AGTACT 1 cut(s) 1140
SchI GAGTC 3 cut(s) 236, 397, 760
ScrFI CCNGG 3 cut(s) 234, 400, 662
SduI GDGCHC 3 cut(s) 629, 959, 1042
SfaNI GCATC 3 cut(s) 144, 601, 666
SfcI CTRYAG 1 cut(s) 918
SfoI GGCGCC 1 cut(s) 379
SfuI TTCGAA 1 cut(s) 1145
SinI GGWCC 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SphI GCATGC 1 cut(s) 98
Sse9I AATT 5 cut(s) 124, 814, 974, 1101, 1120
SsiI CCGC 4 cut(s) 410, 561, 865, 895
SspDI GGCGCC 1 cut(s) 377
SspI AATATT 1 cut(s) 697
SspMI CTAG 4 cut(s) 543, 708, 851, 1028
SstI GAGCTC 1 cut(s) 959
StyD4I CCNGG 3 cut(s) 232, 398, 660
StyI CCWWGG 1 cut(s) 838
TaaI ACNGT 6 cut(s) 83, 337, 622, 633, 919, 970
TaiI ACGT 1 cut(s) 1112
TaqI TCGA 5 cut(s) 47, 225, 267, 1118, 1145
TasI AATT 5 cut(s) 124, 814, 974, 1101, 1120
TatI WGTACW 1 cut(s) 1138
TauI GCSGC 2 cut(s) 867, 897
TfiI GAWTC 6 cut(s) 49, 222, 269, 507, 703, 929
TscAI CASTG 8 cut(s) 188, 201, 321, 360, 484, 625, 761, 1119
TseFI GTSAC 2 cut(s) 205, 739
TseI GCWGC 5 cut(s) 614, 644, 647, 887, 1003
Tsp45I GTSAC 2 cut(s) 205, 739
TspDTI ATGAA 5 cut(s) 16, 129, 620, 808, 1035
TspGWI ACGGA 1 cut(s) 971
TspRI CASTG 8 cut(s) 188, 201, 321, 360, 484, 625, 761, 1119
VpaK11BI GGWCC 1 cut(s) 762
XapI RAATTY 3 cut(s) 814, 974, 1101
XbaI TCTAGA 1 cut(s) 1027
XceI RCATGY 2 cut(s) 98, 517
XspI CTAG 4 cut(s) 543, 708, 851, 1028
ZrmI AGTACT 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.