MD00G1001900.v1.1

Protein BUD31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003415
Physical Location & Seq
Forward (+)
395209 .. 397719
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1001900.v1.1.491

Sequence Viewer

Length: 567 bp
ATGCTTATTCGTACCGATTACGAAAGCCCATTTTGTTCCATCAGTCCTGCCAACAGTGCCTTTCCTCCTCCACACTGCAGCGCTCCACTGCTCCTCCGCTCCTCATTCCCTTCCTCACCTGTAACTAAAATGCCCAAGGTGAAGACTAACCGAGTGAAATACCCACAAGGTTGGGAACTGATTGAGCCTACTCTTCGCGAGCTACAAGCTAAGATGAGAGAAGCTGAGAATGATACTCATGATGGTAAGAGAAAATGTGAGACCTTATGGCCTATTTTCAAAATTGCACATCAAAAGAGCCGCTATATTTTTGACCTTTACCATCGAAGAAAGGAAATTTCCAAGGAATTATATGACTTCTGCCTGGACCAAGGCTATGCTGACCGCAATCTAATTGCAAAGTGGAAAAAGCCTGGATACGAACGCCTCTGTTGTTTAAGATGCATGCAACCTCGGGATCATAACTTTGCCACCACATGTGTTTGCCGAGTGCCCAAGCATCTGAGGGAGGAGAAGGTTATAGAGTGTGTGCATTGTGGCTGTAGGGGCTGTGCCAGTGGAGATTGA

Protein Analysis

189

Amino Acids

21.88

Weight (kDa)

9.03

Isoelectric Point (pI)

62.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BUD31 PF01125 44 - 186 5.7e-65 Pre-mRNA-splicing factor BUD31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 99
AccII CGCG 1 cut(s) 198
AciI CCGC 3 cut(s) 97, 301, 385
AclWI GGATC 1 cut(s) 465
AcsI RAATTY 1 cut(s) 336
AfaI GTAC 1 cut(s) 13
AfeI AGCGCT 1 cut(s) 82
AflIII ACRYGT 1 cut(s) 476
AgsI TTSAA 1 cut(s) 280
AjnI CCWGG 2 cut(s) 363, 412
AluBI AGCT 3 cut(s) 202, 209, 224
AluI AGCT 3 cut(s) 202, 209, 224
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 1 cut(s) 465
Ama87I CYCGRG 1 cut(s) 453
Aor51HI AGCGCT 1 cut(s) 82
AoxI GGCC 1 cut(s) 269
ApeKI GCWGC 1 cut(s) 78
ApoI RAATTY 1 cut(s) 336
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 2 cut(s) 108, 151
AvaI CYCGRG 1 cut(s) 453
AvaII GGWCC 1 cut(s) 367
BaeGI GKGCMC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 90
BccI CCATC 3 cut(s) 47, 236, 330
BciT130I CCWGG 2 cut(s) 365, 414
BciVI GTATCC 1 cut(s) 410
BcoDI GTCTC 1 cut(s) 254
BfmI CTRYAG 2 cut(s) 76, 541
BfoI RGCGCY 1 cut(s) 84
BfuI GTATCC 1 cut(s) 410
BisI GCNGC 2 cut(s) 79, 301
BlsI GCNGC 2 cut(s) 80, 302
Bme1390I CCNGG 2 cut(s) 365, 414
Bme18I GGWCC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 367
BmrFI CCNGG 2 cut(s) 365, 414
BmsI GCATC 2 cut(s) 431, 508
BpiI GAAGAC 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 4 cut(s) 135, 342, 370, 452
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bse1I ACTGG 1 cut(s) 555
BseBI CCWGG 2 cut(s) 365, 414
BseDI CCNNGG 4 cut(s) 135, 342, 370, 452
BseMII CTCAG 2 cut(s) 216, 494
BseNI ACTGG 1 cut(s) 555
BseRI GAGGAG 4 cut(s) 57, 83, 91, 524
BseSI GKGCMC 1 cut(s) 495
BseXI GCAGC 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 1 cut(s) 271
BsiHKCI CYCGRG 1 cut(s) 453
BsmAI GTCTC 1 cut(s) 254
BsnI GGCC 1 cut(s) 271
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 453
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 1 cut(s) 457
Bsp68I TCGCGA 1 cut(s) 198
BspACI CCGC 3 cut(s) 97, 301, 385
BspANI GGCC 1 cut(s) 271
BspCNI CTCAG 2 cut(s) 217, 495
BspFNI CGCG 1 cut(s) 198
BspHI TCATGA 1 cut(s) 238
BspMAI CTGCAG 1 cut(s) 80
BspPI GGATC 1 cut(s) 465
BspTNI GGTCTC 1 cut(s) 254
BsrBI CCGCTC 1 cut(s) 99
BsrI ACTGG 1 cut(s) 555
BssECI CCNNGG 4 cut(s) 135, 342, 370, 452
BssMI GATC 1 cut(s) 457
BssT1I CCWWGG 3 cut(s) 135, 342, 370
Bst2UI CCWGG 2 cut(s) 365, 414
Bst4CI ACNGT 1 cut(s) 56
Bst6I CTCTTC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 200, 446
BstDEI CTNAG 3 cut(s) 210, 225, 503
BstFNI CGCG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 1 cut(s) 457
BstMWI GCNNNNNNNGC 2 cut(s) 56, 546
BstNI CCWGG 2 cut(s) 365, 414
BstNSI RCATGY 2 cut(s) 448, 480
BstSCI CCNGG 2 cut(s) 363, 412
BstSFI CTRYAG 2 cut(s) 76, 541
BstSLI GKGCMC 1 cut(s) 495
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 90
BstV2I GAAGAC 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 171
BsuI GTATCC 1 cut(s) 410
BsuRI GGCC 1 cut(s) 271
BtsI GCAGTG 2 cut(s) 73, 86
BtsIMutI CAGTG 4 cut(s) 61, 73, 86, 562
BtuMI TCGCGA 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 200, 446
CciI TCATGA 1 cut(s) 238
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 367
Csp6I GTAC 1 cut(s) 12
CviAII CATG 3 cut(s) 239, 445, 477
CviQI GTAC 1 cut(s) 12
DdeI CTNAG 3 cut(s) 210, 225, 503
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco130I CCWWGG 3 cut(s) 135, 342, 370
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 367
Eco47III AGCGCT 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 453
EcoRII CCWGG 2 cut(s) 363, 412
EcoT14I CCWWGG 3 cut(s) 135, 342, 370
EcoT22I ATGCAT 1 cut(s) 446
ErhI CCWWGG 3 cut(s) 135, 342, 370
FaeI CATG 3 cut(s) 242, 448, 480
FatI CATG 3 cut(s) 238, 444, 476
Fnu4HI GCNGC 2 cut(s) 79, 301
Fsp4HI GCNGC 2 cut(s) 79, 301
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 79, 301
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 1 cut(s) 271
HhaI GCGC 1 cut(s) 83
Hin1II CATG 3 cut(s) 242, 448, 480
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HphI GGTGA 2 cut(s) 108, 151
Hpy188I TCNGA 1 cut(s) 504
Hpy188III TCNNGA 3 cut(s) 197, 239, 455
HpyAV CCTTC 2 cut(s) 120, 508
HpyCH4III ACNGT 1 cut(s) 56
HpyCH4V TGCA 6 cut(s) 78, 287, 398, 444, 448, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 546
HpyF3I CTNAG 3 cut(s) 210, 225, 503
Hsp92II CATG 3 cut(s) 242, 448, 480
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 3 cut(s) 88, 96, 104
LpnPI CCDG 6 cut(s) 60, 132, 350, 377, 399, 426
Lsp1109I GCAGC 1 cut(s) 90
LweI GCATC 2 cut(s) 431, 508
MaeIII GTNAC 1 cut(s) 121
MalI GATC 1 cut(s) 459
MbiI CCGCTC 1 cut(s) 99
MboI GATC 1 cut(s) 457
MboII GAAGA 3 cut(s) 154, 185, 339
MhlI GDGCHC 1 cut(s) 495
MluCI AATT 4 cut(s) 282, 336, 347, 393
MnlI CCTC 9 cut(s) 75, 78, 104, 112, 124, 437, 462, 498, 502
Mph1103I ATGCAT 1 cut(s) 446
MseI TTAA 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 365, 414
MvaI CCWGG 2 cut(s) 365, 414
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 2 cut(s) 56, 546
NdeII GATC 1 cut(s) 457
NlaIII CATG 3 cut(s) 242, 448, 480
NmeAIII GCCGAG 1 cut(s) 512
NruI TCGCGA 1 cut(s) 198
NsiI ATGCAT 1 cut(s) 446
NspI RCATGY 2 cut(s) 448, 480
PaeI GCATGC 1 cut(s) 448
PagI TCATGA 1 cut(s) 238
PciI ACATGT 1 cut(s) 476
PkrI GCNGC 2 cut(s) 80, 302
PscI ACATGT 1 cut(s) 476
Psp6I CCWGG 2 cut(s) 363, 412
PspGI CCWGG 2 cut(s) 363, 412
PspPI GGNCC 1 cut(s) 367
PstI CTGCAG 1 cut(s) 80
RruI TCGCGA 1 cut(s) 198
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 2 cut(s) 79, 301
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 1 cut(s) 367
ScrFI CCNGG 2 cut(s) 365, 414
SduI GDGCHC 1 cut(s) 495
SfaNI GCATC 2 cut(s) 431, 508
SfcI CTRYAG 2 cut(s) 76, 541
SinI GGWCC 1 cut(s) 367
SphI GCATGC 1 cut(s) 448
Sse9I AATT 4 cut(s) 282, 336, 347, 393
SsiI CCGC 3 cut(s) 97, 301, 385
StyD4I CCNGG 2 cut(s) 363, 412
StyI CCWWGG 3 cut(s) 135, 342, 370
TaaI ACNGT 1 cut(s) 56
TaqI TCGA 1 cut(s) 325
TasI AATT 4 cut(s) 282, 336, 347, 393
TauI GCSGC 1 cut(s) 303
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 4 cut(s) 61, 80, 93, 562
TseI GCWGC 1 cut(s) 78
TspRI CASTG 4 cut(s) 61, 80, 93, 562
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 1 cut(s) 336
XceI RCATGY 2 cut(s) 448, 480
Zsp2I ATGCAT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.