MD06G1015500.v1.1

Protein BUD31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003415
Physical Location & Seq
Forward (+)
1956127 .. 1958223
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1015500.v1.1.491

Sequence Viewer

Length: 354 bp
ATGAGAGAAGCGGAGAATGATACTCACGATGGTAAGAGAAAATGTGAGACCTTGTGGCCTATTTTCAAAATTGCACATCAAAAGAGCCGCTATATTTTCGACCTTTACCATCGAAGAAAGGAAATATCCAAGGAACTATATGAGTTCTGCCTGGACCAAGGCTATGCTGACCGCAATCTAATTGCAAAATGGAAAAAGCCTGGATACGAACGCCTCTGTTGTTTAAGATGCATGCAGCCTCGGGATCATAACTTTGCCACCACATGTGTTTGCCGAGTGCCCAAGCATCTGAGGGAGGAGAAGGTTATAGAGTGTGTGCATTGTGGCTGTAGGGGCTGTGCCAGTGGAGATTGA

Protein Analysis

118

Amino Acids

13.93

Weight (kDa)

8.78

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BUD31 PF01125 1 - 115 2.4e-55 Pre-mRNA-splicing factor BUD31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 11, 88, 172
AclWI GGATC 1 cut(s) 252
AflIII ACRYGT 1 cut(s) 263
AgsI TTSAA 1 cut(s) 67
AjnI CCWGG 2 cut(s) 150, 199
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 252
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 1 cut(s) 56
ApeKI GCWGC 1 cut(s) 235
AspS9I GGNCC 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 247
BccI CCATC 2 cut(s) 23, 117
BciT130I CCWGG 2 cut(s) 152, 201
BciVI GTATCC 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 328
BfuI GTATCC 1 cut(s) 197
BisI GCNGC 2 cut(s) 88, 236
BlsI GCNGC 2 cut(s) 89, 237
Bme1390I CCNGG 2 cut(s) 152, 201
Bme18I GGWCC 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 154
BmrFI CCNGG 2 cut(s) 152, 201
BmsI GCATC 2 cut(s) 218, 295
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 3 cut(s) 129, 157, 239
Bse1I ACTGG 1 cut(s) 342
BseBI CCWGG 2 cut(s) 152, 201
BseDI CCNNGG 3 cut(s) 129, 157, 239
BseMII CTCAG 1 cut(s) 281
BseNI ACTGG 1 cut(s) 342
BseRI GAGGAG 1 cut(s) 311
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 1 cut(s) 247
BshFI GGCC 1 cut(s) 58
BsiHKCI CYCGRG 1 cut(s) 240
BsmAI GTCTC 1 cut(s) 41
BsnI GGCC 1 cut(s) 58
Bso31I GGTCTC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 3 cut(s) 11, 88, 172
BspANI GGCC 1 cut(s) 58
BspCNI CTCAG 1 cut(s) 282
BspPI GGATC 1 cut(s) 252
BspTNI GGTCTC 1 cut(s) 41
BsrI ACTGG 1 cut(s) 342
BssECI CCNNGG 3 cut(s) 129, 157, 239
BssMI GATC 1 cut(s) 244
BssT1I CCWWGG 2 cut(s) 129, 157
Bst2UI CCWGG 2 cut(s) 152, 201
BstC8I GCNNGC 1 cut(s) 233
BstDEI CTNAG 1 cut(s) 290
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 1 cut(s) 333
BstNI CCWGG 2 cut(s) 152, 201
BstNSI RCATGY 2 cut(s) 235, 267
BstSCI CCNGG 2 cut(s) 150, 199
BstSFI CTRYAG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 282
BstV1I GCAGC 1 cut(s) 247
BsuI GTATCC 1 cut(s) 197
BsuRI GGCC 1 cut(s) 58
BtsIMutI CAGTG 1 cut(s) 349
Cac8I GCNNGC 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 154
CviAII CATG 2 cut(s) 232, 264
CviJI RGCY 7 cut(s) 58, 87, 162, 199, 238, 327, 336
CviKI_1 RGCY 7 cut(s) 58, 87, 162, 199, 238, 327, 336
DdeI CTNAG 1 cut(s) 290
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eco130I CCWWGG 2 cut(s) 129, 157
Eco31I GGTCTC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 240
EcoRII CCWGG 2 cut(s) 150, 199
EcoT14I CCWWGG 2 cut(s) 129, 157
EcoT22I ATGCAT 1 cut(s) 233
ErhI CCWWGG 2 cut(s) 129, 157
FaeI CATG 2 cut(s) 235, 267
FaiI YATR 8 cut(s) 93, 139, 141, 165, 233, 249, 265, 308
FatI CATG 2 cut(s) 231, 263
Fnu4HI GCNGC 2 cut(s) 88, 236
Fsp4HI GCNGC 2 cut(s) 88, 236
GluI GCNGC 2 cut(s) 88, 236
HaeIII GGCC 1 cut(s) 58
Hin1II CATG 2 cut(s) 235, 267
Hpy188I TCNGA 1 cut(s) 291
Hpy188III TCNNGA 2 cut(s) 26, 242
HpyAV CCTTC 1 cut(s) 295
HpyCH4V TGCA 5 cut(s) 74, 185, 231, 235, 319
HpyF10VI GCNNNNNNNGC 1 cut(s) 333
HpyF3I CTNAG 1 cut(s) 290
Hsp92II CATG 2 cut(s) 235, 267
Kzo9I GATC 1 cut(s) 244
LpnPI CCDG 4 cut(s) 137, 164, 186, 213
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 2 cut(s) 218, 295
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 2 cut(s) 69, 180
MnlI CCTC 4 cut(s) 224, 249, 285, 289
Mph1103I ATGCAT 1 cut(s) 233
MseI TTAA 1 cut(s) 224
MspR9I CCNGG 2 cut(s) 152, 201
MvaI CCWGG 2 cut(s) 152, 201
MwoI GCNNNNNNNGC 1 cut(s) 333
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 235, 267
NmeAIII GCCGAG 1 cut(s) 299
NsiI ATGCAT 1 cut(s) 233
NspI RCATGY 2 cut(s) 235, 267
PaeI GCATGC 1 cut(s) 235
PciI ACATGT 1 cut(s) 263
PkrI GCNGC 2 cut(s) 89, 237
PscI ACATGT 1 cut(s) 263
Psp6I CCWGG 2 cut(s) 150, 199
PspGI CCWGG 2 cut(s) 150, 199
PspPI GGNCC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 2 cut(s) 88, 236
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 154
ScrFI CCNGG 2 cut(s) 152, 201
SduI GDGCHC 1 cut(s) 282
SetI ASST 3 cut(s) 53, 105, 306
SfaNI GCATC 2 cut(s) 218, 295
SfcI CTRYAG 1 cut(s) 328
SinI GGWCC 1 cut(s) 154
SphI GCATGC 1 cut(s) 235
Sse9I AATT 2 cut(s) 69, 180
SsiI CCGC 3 cut(s) 11, 88, 172
StyD4I CCNGG 2 cut(s) 150, 199
StyI CCWWGG 2 cut(s) 129, 157
TaqI TCGA 2 cut(s) 99, 112
TasI AATT 2 cut(s) 69, 180
TauI GCSGC 1 cut(s) 90
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TscAI CASTG 1 cut(s) 349
TseI GCWGC 1 cut(s) 235
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 154
XceI RCATGY 2 cut(s) 235, 267
Zsp2I ATGCAT 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.