AT4G24450

Pyruvate phosphate dikinase, PEP/pyruvate binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004913
Physical Location & Seq
Forward (+)
12635061 .. 12642808
7748 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24450.3

Sequence Viewer

Length: 3837 bp
ATGGCAACCTCTAAATCCCAACAATTCCAGCTAATTGAAGGGATGGAGCTTCAGATCACCGTCACTGGATTGCCGAATGGGAGCAGTGTAAGAGCTGAGTTTCACCTGAAGAACTGCACTCGCGCATGGATTCTTCATTGGGGTTGTATTTACCAAGGAAATAACCATTGGTATATCCCATCTGAACATTCCTCGAAGCAAGGTGCATTGCAGACTACCTTTGTGAAGAGTGGGGATGCATATGTAGTGATCCTTGAGTTGCGGGATCCAAGGGTACGCGCAATTGAATTTGTTTTGAAAGATGGTAGCCATAACAGATGGTTGAGACAGCATAATGGAAACTTCCGTGTTGAGATTCCCTGGAATGATCTGCATGCTCATCATCGGATACCGAAGACTCTGATAGAAAGAAGAGCACATAAGATATGGGACCGGAAGGGACGACCACAAAGCTCTGCACGTGAACAACAGATAGACTATGACAATGCAGTAAGAGAGCTCCACGCCGAGCTCGCTAGAGGAATATCTTTAGACGAACTTCAGGCTAACTCTACAGTACCAGTGGAGAAAGAAGAAACCAGTGAGCCACATCACACAATGATCCAATCATATCGCCGGAAGCATGATGTTCAGAAATGGTTACAGAAATATACGGAACCAATCAACAGAAGTGGAAGTGTGAAAAGTTCAGCCCTTGCAGAACTCTCCAAGAGATCTGTGGGCCAAGAAAATCTAGTTTCACAGAAAAGCTTTCATGTCAGAAACTACGAGATCACGGTCCTCCAAAGGGATGTTAAGGGAGATTGTCGCTTATGGATTGCCACGAACATGGCAGGTCCAACAGTTCTCCATTGGGGAGTCGCAAAGTCATCTGCAGGAGAGTGGTTGATACCACCACCTGACGTGTTACCTGAGAAATCAAAATTTGTTCACGGAGCATGTCAAACACAATTTACCGATATGTCGAGCAGAGAACACTCTTATCAGTTTATTGATATAAATTTAAAACGAGGTGGTTTTGTTGGTATCCAATTTGTAATATGGTCGGGAGGCTACTGGGTGAACAATAACGGAGCCAACTTCGTTGTAAACCTGAAGTCAGCAGACAGTACCAGTGGTAAGCTTGACGTGGATGAAAAGTATGTTCTCAAATGGTTACTTGATGAAATATCTGAGCGAGAGAAAGAGGCTGAGAGATCATTGATGCACAGGTTCAACATTGCAACAGAGTTGACCGAGCGTTGTAAGGATGAAGGAGAAGGTGGATGTATTGGTATAATGGTGTGGATGAGATTCATGGCCACCAGACATCTCACCTGGAATAAGAACTATAACGTGAAACCTCGGGAAATTAGTGAAGCACTAGAAAGATTCACCAATTTGATGGAGAAAATATATTTGCAGCAACCAAATAAGAGAGAAATTGTGAGACTAACTATGGCACTTGTGGGTCGTGGAGGTCAAGGTGATGTTGGGCAGAGAATCCGTGACGAAATCCTTGTTATTCAGAGAAATAACCACTGCAAATCCGGCATGATGGAAGAGTGGCACCAAAAGTTGCACAACAACAGTAGCGCAGATGATGTGATAATTTGTGAGGCTCTCTTGAACTATGTGAGATCTGACTTCAGGATCGATGCATACTGGCAAACACTACAGACCAATGGTCTCACAAAAGAAAGGCTTGCAAGTTATGACCGCCCCATAGTATCAGAGCCTCGTTTCAGAAGTGATTCTAAAGAAGGACTCATCCGTGACCTTACAATGTACTTGAAAACATTAAAGGCAGTTCATTCAGGTGCAGACCTTGAGTCTGCTATTGATACGTTCCTTTCTCCATCTAAGGGTCATCATGTCTTTGCTGTCAATGGTTTATCACCAAAATTGCAGGATCTACTGAATTTAGTTAAGAGGCTTGTTCGCGAAGAGAACACTGAGCCCCTAATAGAGAAGTTAGTTGATGCTCGCATTCAGTTACACCCTGCACTACGAGCACCTCGCACGAGGGCAAAAGATTTACTATTTTTGGACATTGCGTTGGAGTCATGTTTTAAAACAACAATAGAGAAGAGACTCATCTCTTTAAACTTCAATAACCCACCGGAAATTATATATGTCATCTGCGTGGTGCTTGAGAATCTGTGCTTATCTATAGTTAACAATGAAGAAATCATATTCTGTACAAAGGATTGGTACCGTGTCAGCGAGGCTTACAGACCTCATGATGTTCAGTGGGCATTGCAAACAAAAGCGGTCCTTGACCGTTTACAACTAGTACTTGCTGATAGATGTCAGCATTATTTTACAATAATACAGCCTACTGCAAAATATCTTGGTCAACTGTTACGTGTTGACAAGCATGGGATTGATGTTTTCACTGAAGAGGTTATAAGAGCAGGGCCAGGAGCCGTTTTATCAACTCTTGTAAACAGATTTGATCCTTCTCTAAGGAAAATCGCGAATTTAGGCTGTTGGCAGGTTATCAGCTCGGCTGATGCATATGGGTTTGTGGTTTGTGTGAATGAGTTAATTGTTGTTCAGAACAAATTCTACTCGAAGCCAACTGTAATTATTGCAAGTAAAGTCACAGGAGAAGAGGAGATCCCTGCTGGTGTTGTAGCCGTGCTGACTCCTAGTATGATTGATGTCTTGTCTCATGTATCTATTAGAGCAAGAAACAGCAAGATATGCTTTGCTACATGCTTCGATCAGAATGTTCTCAGCAATCTGAAGTCGAAGGAAGGGAGAGCAATATCTATTCATACAAAGTCTACTGGTTTAGTTATCAGTGATGGCAACAACTCTGATGTCTCTGTTCGTCATATTTTTATTTCCTCTGTTCCACGGGGAGTGATCTCTAAGGGGAAGAAGTTTTGTGGCCACTATGTGATTTCATCTAAGGAATTCACTGATGAAAGGGTTGGCTCAAAATCATACAATATAAAATTTCTACGTGAAAGAGTTCCATCATGGATCAAGATACCTACCTCAGCTGCTCTTCCATTCGGAACATTTGAGAATATACTCTCAGATGATTCCAATAAGGATGTAGCACGCAGGATTTCTGTCCTTAAAGATTCTCTTAACAGAGGAGACCTGACAAAACTTAAGTCCATTCAAGAAGCTATCTTACAAATGAGTGCTCCAATGGCTCTGAGAAATGAACTGATCACAAAATTGAGAAGTGAAAGAATGCCTTATCTTGGTGATGAATCAGGCTGGAACCGCTCCTGGGTGGCAATTAAGAAGGTCTGGGCTTCAAAGTGGAACGAGAGAGCTTATGTCAGTTGCAAAAAAAATAAGCTTGATCATGATGCGGTCTGCATGGCTGTGCTGATTCAAGAAGTCATTTGTGGCGATTATGCTTTCGTCATTCACACAAACAATCCGGTCTCTGGTGACTCTTCAGAAATATACACAGAGATTGTGAAGGGTTTGGGAGAGACCTTGGTTGGAGCATATCCAGGACGAGCAATGAGCTTCATCACCAAGAAAACAAACCTCAAGTCTCCAACCGTGATCAGTTACCCAAGTAAGAGGATAGGTCTGTATTCTAAACCCTCAATCATATTCAGATCAGATTCAAACAACGAGGATCTCGAAGGCAACGCAGGCGCTGGACTTTACGACAGTGTGATAATGGATGAAGCAGAGGAAGTAGTGGTGGATTACTCAAGGGAGCCACTAATAATGGACAAATCCTTTCGAGTGCGTCTATTCTCAGCGATTGCAGAAGCTGGAAATGTGATAGAGTCAATCTATGGTTGTCCTCAAGACATTGAAGGTGTTGTCAAAGGTGGACATATCTACATCGTCCAAGCTAGACCCCAAGTTTAA

Protein Analysis

1278

Amino Acids

144.81

Weight (kDa)

8.75

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_N_CWD1 PF23166 6 - 119 1.5e-28 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067 PF23229 134 - 185 8.3e-17 Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1 PF23166 255 - 365 8.3e-23 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD PF22973 824 - 936 5.6e-47 Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N PF01326 1079 - 1277 1.3e-21 Pyruvate phosphate dikinase, AMP/ATP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2390
Acc36I ACCTGC 2 cut(s) 824, 2467
Acc65I GGTACC 1 cut(s) 2193
AccB1I GGYRCC 2 cut(s) 1548, 2193
AccBSI CCGCTC 1 cut(s) 3228
AccI GTMKAC 1 cut(s) 2771
AccII CGCG 4 cut(s) 123, 279, 1923, 2459
AciI CCGC 5 cut(s) 262, 1699, 2252, 3226, 3317
AcoI YGGCCR 2 cut(s) 1299, 2878
AcsI RAATTY 8 cut(s) 287, 923, 1000, 1900, 2461, 2546, 2903, 2945
AcuI CTGAAG 8 cut(s) 35, 128, 524, 1115, 1612, 2400, 2750, 3390
AcvI CACGTG 1 cut(s) 461
AdeI CACNNNGTG 1 cut(s) 2887
AfaI GTAC 7 cut(s) 276, 558, 1111, 1769, 2182, 2195, 2277
AfiI CCNNNNNNNGG 5 cut(s) 787, 1844, 3203, 3232, 3395
AflII CTTAAG 1 cut(s) 3107
AflIII ACRYGT 2 cut(s) 903, 2347
AhdI GACNNNNNGTC 2 cut(s) 1665, 1810
AhlI ACTAGT 1 cut(s) 2272
AjiI CACGTC 2 cut(s) 904, 1129
AjnI CCWGG 5 cut(s) 359, 1316, 2401, 3230, 3463
AjuI GAANNNNNNNTTGG 2 cut(s) 151, 183
Alw21I GWGCWC 5 cut(s) 418, 501, 513, 1996, 3145
AlwNI CAGNNNCTG 2 cut(s) 3617, 3737
Ama87I CYCGRG 1 cut(s) 1344
AoxI GGCC 4 cut(s) 721, 1299, 2399, 2878
ApeKI GCWGC 2 cut(s) 1402, 2993
ApoI RAATTY 8 cut(s) 287, 923, 1000, 1900, 2461, 2546, 2903, 2945
ArsI GACNNNNNNTTYG 4 cut(s) 3095, 3127, 3491, 3523
Asp700I GAANNNNTTC 3 cut(s) 1732, 2546, 2961
Asp718I GGTACC 1 cut(s) 2193
AspLEI GCGC 4 cut(s) 125, 281, 1577, 3617
AspS9I GGNCC 6 cut(s) 430, 721, 778, 836, 2254, 2399
AvaI CYCGRG 1 cut(s) 1344
AvaII GGWCC 4 cut(s) 430, 778, 836, 2254
BalI TGGCCA 2 cut(s) 1301, 2880
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 2 cut(s) 1548, 2193
BanII GRGCYC 3 cut(s) 501, 513, 1941
BarI GAAGNNNNNNTAC 2 cut(s) 3114, 3146
BauI CACGAG 1 cut(s) 2002
BbrPI CACGTG 1 cut(s) 461
BbsI GAAGAC 1 cut(s) 401
Bbv12I GWGCWC 5 cut(s) 418, 501, 513, 1996, 3145
BbvCI CCTCAGC 1 cut(s) 2989
BbvI GCAGC 2 cut(s) 1414, 2980
BccI CCATC 9 cut(s) 37, 187, 296, 312, 1378, 1531, 1846, 2786, 2974
BceAI ACGGC 2 cut(s) 2393, 2606
BciT130I CCWGG 5 cut(s) 361, 1318, 2403, 3232, 3465
BciVI GTATCC 2 cut(s) 381, 1037
BclI TGATCA 3 cut(s) 3168, 3307, 3519
BcuI ACTAGT 1 cut(s) 2272
BfaI CTAG 6 cut(s) 516, 734, 1364, 2273, 2634, 3822
BfmI CTRYAG 4 cut(s) 552, 873, 1655, 2151
BfoI RGCGCY 1 cut(s) 3618
BfrI CTTAAG 1 cut(s) 3107
BfuAI ACCTGC 2 cut(s) 824, 2467
BfuI GTATCC 2 cut(s) 381, 1037
BglII AGATCT 2 cut(s) 713, 1619
BisI GCNGC 2 cut(s) 1403, 2994
BlsI GCNGC 2 cut(s) 1404, 2995
BmcAI AGTACT 1 cut(s) 2277
Bme1390I CCNGG 5 cut(s) 361, 1318, 2403, 3232, 3465
Bme18I GGWCC 4 cut(s) 430, 778, 836, 2254
BmeRI GACNNNNNGTC 2 cut(s) 1665, 1810
BmeT110I CYCGRG 1 cut(s) 1344
BmgBI CACGTC 2 cut(s) 904, 1129
BmgT120I GGNCC 6 cut(s) 430, 721, 778, 836, 2254, 2399
BmiI GGNNCC 9 cut(s) 267, 431, 657, 1075, 1550, 2195, 2407, 3224, 3681
BmrFI CCNGG 5 cut(s) 361, 1318, 2403, 3232, 3465
BmrI ACTGGG 1 cut(s) 1066
BmsI GCATC 6 cut(s) 226, 1194, 1627, 1951, 2485, 3304
BmuI ACTGGG 1 cut(s) 1066
BpiI GAAGAC 1 cut(s) 401
Bpu10I CCTNAGC 1 cut(s) 2989
BpuEI CTTGAG 6 cut(s) 275, 1829, 2153, 3488, 3658, 3756
Bsa29I ATCGAT 1 cut(s) 1635
BsaAI YACGTR 3 cut(s) 461, 2348, 2954
BsaI GGTCTC 4 cut(s) 1673, 3087, 3397, 3437
BsaJI CCNNGG 7 cut(s) 154, 269, 359, 1343, 2843, 3231, 3447
BsaWI WCCGGW 3 cut(s) 432, 2101, 3388
Bsc4I CCNNNNNNNGG 5 cut(s) 787, 1844, 3203, 3232, 3395
Bse1I ACTGG 7 cut(s) 70, 560, 579, 1061, 1113, 1649, 2779
Bse3DI GCAATG 5 cut(s) 206, 1218, 2031, 2237, 3480
BseBI CCWGG 5 cut(s) 361, 1318, 2403, 3232, 3465
BseCI ATCGAT 1 cut(s) 1635
BseDI CCNNGG 7 cut(s) 154, 269, 359, 1343, 2843, 3231, 3447
BseLI CCNNNNNNNGG 5 cut(s) 787, 1844, 3203, 3232, 3395
BseMI GCAATG 5 cut(s) 206, 1218, 2031, 2237, 3480
BseNI ACTGG 7 cut(s) 70, 560, 579, 1061, 1113, 1649, 2779
BseRI GAGGAG 2 cut(s) 2612, 3105
BseXI GCAGC 2 cut(s) 1414, 2980
BsgI GTGCAG 4 cut(s) 100, 441, 1821, 1968
Bsh1236I CGCG 4 cut(s) 123, 279, 1923, 2459
BshFI GGCC 4 cut(s) 723, 1301, 2401, 2880
BshNI GGYRCC 2 cut(s) 1548, 2193
BshVI ATCGAT 1 cut(s) 1635
BsiHKAI GWGCWC 5 cut(s) 418, 501, 513, 1996, 3145
BsiHKCI CYCGRG 1 cut(s) 1344
BsiSI CCGG 5 cut(s) 433, 616, 1530, 2102, 3389
BslFI GGGAC 2 cut(s) 443, 453
BslI CCNNNNNNNGG 5 cut(s) 787, 1844, 3203, 3232, 3395
BsmFI GGGAC 2 cut(s) 443, 453
BsmI GAATGC 2 cut(s) 1968, 3198
BsnI GGCC 4 cut(s) 723, 1301, 2401, 2880
Bso31I GGTCTC 4 cut(s) 1673, 3087, 3397, 3437
BsoBI CYCGRG 1 cut(s) 1344
Bsp1286I GDGCHC 6 cut(s) 418, 501, 513, 1941, 1996, 3145
Bsp1407I TGTACA 1 cut(s) 2180
Bsp68I TCGCGA 2 cut(s) 1923, 2459
BspACI CCGC 5 cut(s) 262, 1699, 2252, 3226, 3317
BspANI GGCC 4 cut(s) 723, 1301, 2401, 2880
BspDI ATCGAT 1 cut(s) 1635
BspFNI CGCG 4 cut(s) 123, 279, 1923, 2459
BspHI TCATGA 2 cut(s) 2221, 3310
BspLI GGNNCC 9 cut(s) 267, 431, 657, 1075, 1550, 2195, 2407, 3224, 3681
BspMAI CTGCAG 1 cut(s) 877
BspMI ACCTGC 2 cut(s) 824, 2467
BspQI GCTCTTC 2 cut(s) 406, 3003
BspT107I GGYRCC 2 cut(s) 1548, 2193
BspTI CTTAAG 1 cut(s) 3107
BspTNI GGTCTC 4 cut(s) 1673, 3087, 3397, 3437
BsrBI CCGCTC 1 cut(s) 3228
BsrDI GCAATG 5 cut(s) 206, 1218, 2031, 2237, 3480
BsrGI TGTACA 1 cut(s) 2180
BsrI ACTGG 7 cut(s) 70, 560, 579, 1061, 1113, 1649, 2779
BssECI CCNNGG 7 cut(s) 154, 269, 359, 1343, 2843, 3231, 3447
BssSI CACGAG 1 cut(s) 2002
BssT1I CCWWGG 3 cut(s) 154, 269, 3447
Bst2BI CACGAG 1 cut(s) 2002
Bst2UI CCWGG 5 cut(s) 361, 1318, 2403, 3232, 3465
Bst6I CTCTTC 9 cut(s) 221, 406, 1536, 1920, 2063, 2376, 2589, 3003, 3409
BstAFI CTTAAG 1 cut(s) 3107
BstAPI GCANNNNNTGC 1 cut(s) 2688
BstAUI TGTACA 1 cut(s) 2180
BstBAI YACGTR 3 cut(s) 461, 2348, 2954
BstC8I GCNNGC 6 cut(s) 375, 513, 1686, 1966, 3055, 3613
BstDSI CCRYGG 1 cut(s) 2843
BstFNI CGCG 4 cut(s) 123, 279, 1923, 2459
BstH2I RGCGCY 1 cut(s) 3618
BstHHI GCGC 4 cut(s) 125, 281, 1577, 3617
BstMWI GCNNNNNNNGC 7 cut(s) 512, 1530, 1991, 2688, 3149, 3225, 3612
BstNI CCWGG 5 cut(s) 361, 1318, 2403, 3232, 3465
BstNSI RCATGY 3 cut(s) 377, 942, 2703
BstSCI CCNGG 5 cut(s) 359, 1316, 2401, 3230, 3463
BstSFI CTRYAG 4 cut(s) 552, 873, 1655, 2151
BstUI CGCG 4 cut(s) 123, 279, 1923, 2459
BstV1I GCAGC 2 cut(s) 1414, 2980
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 6 cut(s) 265, 713, 1619, 1891, 2601, 3595
BstXI CCANNNNNNTGG 2 cut(s) 829, 1384
BstYI RGATCY 6 cut(s) 265, 713, 1619, 1891, 2601, 3595
Bsu15I ATCGAT 1 cut(s) 1635
BsuI GTATCC 2 cut(s) 381, 1037
BsuRI GGCC 4 cut(s) 723, 1301, 2401, 2880
BsuTUI ATCGAT 1 cut(s) 1635
BtgI CCRYGG 1 cut(s) 2843
BtrI CACGTC 2 cut(s) 904, 1129
BtsI GCAGTG 2 cut(s) 91, 1519
BtuMI TCGCGA 2 cut(s) 1923, 2459
BveI ACCTGC 2 cut(s) 824, 2467
Cac8I GCNNGC 6 cut(s) 375, 513, 1686, 1966, 3055, 3613
CaiI CAGNNNCTG 2 cut(s) 3617, 3737
CciI TCATGA 2 cut(s) 2221, 3310
CfoI GCGC 4 cut(s) 125, 281, 1577, 3617
Cfr13I GGNCC 6 cut(s) 430, 721, 778, 836, 2254, 2399
ClaI ATCGAT 1 cut(s) 1635
CseI GACGC 1 cut(s) 3701
Csp6I GTAC 7 cut(s) 275, 557, 1110, 1768, 2181, 2194, 2276
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviQI GTAC 7 cut(s) 275, 557, 1110, 1768, 2181, 2194, 2276
DraI TTTAAA 3 cut(s) 1005, 2053, 2085
DraIII CACNNNGTG 1 cut(s) 2887
DriI GACNNNNNGTC 2 cut(s) 1665, 1810
EaeI YGGCCR 2 cut(s) 1299, 2878
Eam1104I CTCTTC 9 cut(s) 221, 406, 1536, 1920, 2063, 2376, 2589, 3003, 3409
Eam1105I GACNNNNNGTC 2 cut(s) 1665, 1810
EarI CTCTTC 9 cut(s) 221, 406, 1536, 1920, 2063, 2376, 2589, 3003, 3409
Ecl136II GAGCTC 2 cut(s) 499, 511
Eco130I CCWWGG 3 cut(s) 154, 269, 3447
Eco24I GRGCYC 3 cut(s) 501, 513, 1941
Eco31I GGTCTC 4 cut(s) 1673, 3087, 3397, 3437
Eco47I GGWCC 4 cut(s) 430, 778, 836, 2254
Eco53kI GAGCTC 2 cut(s) 499, 511
Eco57I CTGAAG 8 cut(s) 35, 128, 524, 1115, 1612, 2400, 2750, 3390
Eco72I CACGTG 1 cut(s) 461
Eco88I CYCGRG 1 cut(s) 1344
EcoICRI GAGCTC 2 cut(s) 499, 511
EcoRI GAATTC 1 cut(s) 2903
EcoRII CCWGG 5 cut(s) 359, 1316, 2401, 3230, 3463
EcoT14I CCWWGG 3 cut(s) 154, 269, 3447
EcoT22I ATGCAT 3 cut(s) 241, 1642, 2500
EcoT38I GRGCYC 3 cut(s) 501, 513, 1941
ErhI CCWWGG 3 cut(s) 154, 269, 3447
FaqI GGGAC 2 cut(s) 443, 453
FauI CCCGC 1 cut(s) 255
FauNDI CATATG 2 cut(s) 241, 2500
FbaI TGATCA 3 cut(s) 3168, 3307, 3519
FblI GTMKAC 1 cut(s) 2771
Fnu4HI GCNGC 2 cut(s) 1403, 2994
FriOI GRGCYC 3 cut(s) 501, 513, 1941
Fsp4HI GCNGC 2 cut(s) 1403, 2994
FspBI CTAG 6 cut(s) 516, 734, 1364, 2273, 2634, 3822
GlaI GCGC 4 cut(s) 124, 280, 1576, 3616
GluI GCNGC 2 cut(s) 1403, 2994
HaeII RGCGCY 1 cut(s) 3618
HaeIII GGCC 4 cut(s) 723, 1301, 2401, 2880
HapII CCGG 5 cut(s) 433, 616, 1530, 2102, 3389
HgaI GACGC 1 cut(s) 3701
HhaI GCGC 4 cut(s) 125, 281, 1577, 3617
Hin6I GCGC 4 cut(s) 123, 279, 1575, 3615
HinP1I GCGC 4 cut(s) 123, 279, 1575, 3615
HincII GTYRAC 4 cut(s) 1233, 2158, 2339, 2353
HindII GTYRAC 4 cut(s) 1233, 2158, 2339, 2353
HindIII AAGCTT 3 cut(s) 748, 1121, 3302
HpaI GTTAAC 1 cut(s) 2158
HpaII CCGG 5 cut(s) 433, 616, 1530, 2102, 3389
HpyCH4IV ACGT 7 cut(s) 460, 903, 1128, 1335, 1826, 2347, 2953
HpyF10VI GCNNNNNNNGC 7 cut(s) 512, 1530, 1991, 2688, 3149, 3225, 3612
HpySE526I ACGT 7 cut(s) 460, 903, 1128, 1335, 1826, 2347, 2953
HspAI GCGC 4 cut(s) 123, 279, 1575, 3615
KpnI GGTACC 1 cut(s) 2197
Ksp22I TGATCA 3 cut(s) 3168, 3307, 3519
KspAI GTTAAC 1 cut(s) 2158
LguI GCTCTTC 2 cut(s) 406, 3003
Lsp1109I GCAGC 2 cut(s) 1414, 2980
LweI GCATC 6 cut(s) 226, 1194, 1627, 1951, 2485, 3304
MaeI CTAG 6 cut(s) 516, 734, 1364, 2273, 2634, 3822
MaeII ACGT 7 cut(s) 460, 903, 1128, 1335, 1826, 2347, 2953
MbiI CCGCTC 1 cut(s) 3228
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 6 cut(s) 265, 713, 1619, 1891, 2601, 3595
MhlI GDGCHC 6 cut(s) 418, 501, 513, 1941, 1996, 3145
MlsI TGGCCA 2 cut(s) 1301, 2880
MluNI TGGCCA 2 cut(s) 1301, 2880
MlyI GAGTC 9 cut(s) 391, 867, 1740, 1820, 2051, 2067, 2623, 3395, 3761
MmeI TCCRAC 4 cut(s) 863, 2019, 3433, 3536
Mox20I TGGCCA 2 cut(s) 1301, 2880
Mph1103I ATGCAT 3 cut(s) 241, 1642, 2500
MroXI GAANNNNTTC 3 cut(s) 1732, 2546, 2961
MscI TGGCCA 2 cut(s) 1301, 2880
MslI CAYNNNNRTG 4 cut(s) 827, 1797, 2123, 3329
Msp20I TGGCCA 2 cut(s) 1301, 2880
MspA1I CMGCKG 1 cut(s) 2993
MspCI CTTAAG 1 cut(s) 3107
MspI CCGG 5 cut(s) 433, 616, 1530, 2102, 3389
MspR9I CCNGG 5 cut(s) 361, 1318, 2403, 3232, 3465
MunI CAATTG 1 cut(s) 282
Mva1269I GAATGC 2 cut(s) 1968, 3198
MvaI CCWGG 5 cut(s) 361, 1318, 2403, 3232, 3465
MvnI CGCG 4 cut(s) 123, 279, 1923, 2459
MwoI GCNNNNNNNGC 7 cut(s) 512, 1530, 1991, 2688, 3149, 3225, 3612
NdeI CATATG 2 cut(s) 241, 2500
NlaIV GGNNCC 9 cut(s) 267, 431, 657, 1075, 1550, 2195, 2407, 3224, 3681
NmeAIII GCCGAG 2 cut(s) 532, 2468
NmuCI GTSAC 5 cut(s) 61, 1487, 1754, 2584, 3398
NruI TCGCGA 2 cut(s) 1923, 2459
NsiI ATGCAT 3 cut(s) 241, 1642, 2500
NspI RCATGY 3 cut(s) 377, 942, 2703
PaeI GCATGC 1 cut(s) 377
PagI TCATGA 2 cut(s) 2221, 3310
PciSI GCTCTTC 2 cut(s) 406, 3003
PcsI WCGNNNNNNNCGW 1 cut(s) 3597
PctI GAATGC 2 cut(s) 1968, 3198
PdmI GAANNNNTTC 3 cut(s) 1732, 2546, 2961
PfoI TCCNGGA 1 cut(s) 3463
PkrI GCNGC 2 cut(s) 1404, 2995
PleI GAGTC 9 cut(s) 391, 866, 1740, 1819, 2050, 2067, 2623, 3395, 3760
PmaCI CACGTG 1 cut(s) 461
PmlI CACGTG 1 cut(s) 461
PpsI GAGTC 9 cut(s) 391, 866, 1740, 1819, 2050, 2067, 2623, 3395, 3760
Ppu21I YACGTR 3 cut(s) 461, 2348, 2954
PsiI TTATAA 1 cut(s) 2390
Psp124BI GAGCTC 2 cut(s) 501, 513
Psp6I CCWGG 5 cut(s) 359, 1316, 2401, 3230, 3463
PspCI CACGTG 1 cut(s) 461
PspGI CCWGG 5 cut(s) 359, 1316, 2401, 3230, 3463
PspN4I GGNNCC 9 cut(s) 267, 431, 657, 1075, 1550, 2195, 2407, 3224, 3681
PspPI GGNCC 6 cut(s) 430, 721, 778, 836, 2254, 2399
PsrI GAACNNNNNNTAC 2 cut(s) 2534, 2566
PstI CTGCAG 1 cut(s) 877
PstNI CAGNNNCTG 2 cut(s) 3617, 3737
PsuI RGATCY 6 cut(s) 265, 713, 1619, 1891, 2601, 3595
PvuII CAGCTG 1 cut(s) 2993
RruI TCGCGA 2 cut(s) 1923, 2459
RsaI GTAC 7 cut(s) 276, 558, 1111, 1769, 2182, 2195, 2277
RsaNI GTAC 7 cut(s) 275, 557, 1110, 1768, 2181, 2194, 2276
RseI CAYNNNNRTG 4 cut(s) 827, 1797, 2123, 3329
SacI GAGCTC 2 cut(s) 501, 513
SapI GCTCTTC 2 cut(s) 406, 3003
SatI GCNGC 2 cut(s) 1403, 2994
Sau96I GGNCC 6 cut(s) 430, 721, 778, 836, 2254, 2399
ScaI AGTACT 1 cut(s) 2277
SchI GAGTC 9 cut(s) 391, 867, 1740, 1820, 2051, 2067, 2623, 3395, 3761
ScrFI CCNGG 5 cut(s) 361, 1318, 2403, 3232, 3465
SduI GDGCHC 6 cut(s) 418, 501, 513, 1941, 1996, 3145
SfaNI GCATC 6 cut(s) 226, 1194, 1627, 1951, 2485, 3304
SfcI CTRYAG 4 cut(s) 552, 873, 1655, 2151
SinI GGWCC 4 cut(s) 430, 778, 836, 2254
SmiMI CAYNNNNRTG 4 cut(s) 827, 1797, 2123, 3329
SmlI CTYRAG 7 cut(s) 254, 1808, 2132, 3107, 3503, 3673, 3771
SmoI CTYRAG 7 cut(s) 254, 1808, 2132, 3107, 3503, 3673, 3771
SpeI ACTAGT 1 cut(s) 2272
SphI GCATGC 1 cut(s) 377
SsiI CCGC 5 cut(s) 262, 1699, 2252, 3226, 3317
SspMI CTAG 6 cut(s) 516, 734, 1364, 2273, 2634, 3822
SstI GAGCTC 2 cut(s) 501, 513
StyD4I CCNGG 5 cut(s) 359, 1316, 2401, 3230, 3463
StyI CCWWGG 3 cut(s) 154, 269, 3447
TaiI ACGT 7 cut(s) 463, 906, 1131, 1338, 1829, 2350, 2956
TaqI TCGA 8 cut(s) 194, 965, 1635, 2555, 2706, 2735, 3600, 3706
TaqII GACCGA 1 cut(s) 1250
TatI WGTACW 3 cut(s) 1767, 2180, 2275
TseFI GTSAC 5 cut(s) 61, 1487, 1754, 2584, 3398
TseI GCWGC 2 cut(s) 1402, 2993
Tsp45I GTSAC 5 cut(s) 61, 1487, 1754, 2584, 3398
TspGWI ACGGA 6 cut(s) 335, 668, 948, 1086, 1475, 1742
Vha464I CTTAAG 1 cut(s) 3107
VpaK11BI GGWCC 4 cut(s) 430, 778, 836, 2254
XapI RAATTY 8 cut(s) 287, 923, 1000, 1900, 2461, 2546, 2903, 2945
XceI RCATGY 3 cut(s) 377, 942, 2703
XcmI CCANNNNNNNNNTGG 1 cut(s) 715
XmiI GTMKAC 1 cut(s) 2771
XmnI GAANNNNTTC 3 cut(s) 1732, 2546, 2961
XspI CTAG 6 cut(s) 516, 734, 1364, 2273, 2634, 3822
ZrmI AGTACT 1 cut(s) 2277
Zsp2I ATGCAT 3 cut(s) 241, 1642, 2500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.