Rorug07G0283000

water dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0004913
Physical Location & Seq
Reverse (-)
27464768 .. 27467726
2959 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0283000.1

Sequence Viewer

Length: 1635 bp
ATGGCGGAACCCCAAATGTCCAAGACCTTCCCCGTGAAACCGAAGCAAAAAGCCAAGCCTCGCACCCCAAAACAAACCGCAGAATCCAAGTACTGGTCCTCCTTCAAAACCAAGCAAATCCCTAATCTCATCGCCTCCATAAACTCCCTCACCTTCTCCCCCACCGCCCCTCACTCCTTCGCCGCCGCCCACTCCACCTCCCTCACCCTCTACAACCCCCAAAACCAATTCTCCCGCTCCTCCAACATCTCCCTAAACGACGTCGTCTCCTCCGCCTCCTTCCGCTCCGACGGCCTCCTCATCGCCGCCGCCGACCTCTCCGGCCTCGTCCAAGTCTTCGATGTCAAAACCCGAACCCCCCTCCGCAGGCTCCGGTCCCACACCCGCCCCGTCCGCTTCGTCAAGTTCCCCCTCGCCGATAAGCTCCACTTGGTCTCCGGCGGCGACGACGCGGTCGTCAAGTACTGGGACGTCGCCGGCGAGACCACGATTTGCGACTTCCTAGGGCACAAGGACTACGTGCGCTGCGGCGATTGGTCCCCAAATGATTCCAACATGTTCATCACTGGGTCGTATGATCACACAGTGAAGCTCTGGGATGTGAGGGATAAGGATTCGAGCTCCGTGACGGAAGTGAATCACGGTAAGCCGGTGGAGGACGTGATGTTCTTGCCTTCCGGCGGCTTAATTGCGACGGCCGGAGGAGACTCGGTGAAGATTTGGGATTTGATTGGAGGTGGGAAGCTGGTGTATTCAATGGAGAGCCACAACAAGACGGTGACTTCGATTTGTGTTGGGAAAGTTGGGAAGCTCAGTGGGGAAGAGGCTCAGCAGTACAGGATTTTGAGTGTGGCATTGGATGGTTACATGAAGGTTTTCGATTACGCGAAAATGAAGGTTACTCATTCAATGAGGTTCCCTGCGCCGCTCATGTCAGTGGGGTTTTCGCCGGATTGTATGACTAGGGTTATAGGAACTTCGAATGGGATAATATATGCTGGGAGGAGGAAAAACAAGGAAGATGTGGAGAGTGGTTCTGATGAGGAGCTTACCAAGTATGAAAGGAGGTTGAAGAAGTTGGGTTCCGGGAATGCTTTGGGTTTGGGGCCTGTGGCGGAGGAGCCACGGATTAGGGTCTTGAAGCCTACTAGTTTTCGGTATTTTCAGAGAGGGCAGTCAGAGAAGCCGTCTGAGAGAGATTATCTGGTGATGAGGCCGAAGAAGGTGAAGTTGGGGGAGCATGATAAGCTGTTGAAGAAGTTTAGGCATAAGGAAGCTCTGGTGTCTGTATTGGGGAGGACTAAGAATCCGGAGAATGCTGTGGCTGTGATGGAAGAATTGGTGTCGAGAAAGAAGTTGCTGAAATGTGTTTCGAATTTGGATTTGGAGGAGCTCGGAATGTTGCTGATGTTTTTGCAGAGGTACTCGACCATGCCAAGGTACTCGAGGTTGTTGATGGGGTTGACAGAGAAGGTTATTGAGATGAGGGCTGACGACATTAGGGCTTCTACAGAGTTGAGGGGTCTTATGAGAAACATTAAACGTGCTGTCGAGGAGGAAATACGAATACAACAATCTTTGCAAGAGATACAGGGTGTGATTTCTCCTTTGTTGAGGATAACTGGAAGAAGATGA

Protein Analysis

544

Amino Acids

60.97

Weight (kDa)

9.66

Isoelectric Point (pI)

47.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 42 - 157 1e-10 Prp19 WD40 domain
WDR55 PF24796 45 - 175 4.3e-08 WDR55
Beta-prop_THOC3 PF25174 45 - 159 1.5e-09 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 48 - 262 2.1e-20 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 49 - 183 3.9e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 74 - 243 5.8e-23 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 77 - 171 7.1e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 87 - 262 2.1e-29 WDR5 beta-propeller domain
WD40_Gbeta PF25391 89 - 271 1.3e-15 G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd PF24782 99 - 264 9.6e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EIPR1 PF23609 100 - 199 2e-07 EIPR1 beta-propeller
Beta-prop_EML PF23409 108 - 332 3.4e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR41 PF25178 110 - 242 4.9e-07 WDR41
Beta-prop_CAF1B_HIR1 PF24105 123 - 238 6.5e-06 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 124 - 232 5.7e-08 EIF3I
Beta-prop_THOC3 PF25174 127 - 245 6.5e-17 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 128 - 209 4e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 143 - 237 1.5e-12 Prp19 WD40 domain
Beta-prop_RIG_2nd PF23775 163 - 230 1.5e-07 RIG second beta-propeller
WD40 PF00400 165 - 200 1.8e-07 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 165 - 271 5.7e-08 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 169 - 301 3.4e-07 TEP-1 second beta-propeller
Beta-prop_EIPR1 PF23609 169 - 256 3.8e-06 EIPR1 beta-propeller
WD40_Prp19 PF24814 174 - 331 1.2e-12 Prp19 WD40 domain
WDR55 PF24796 183 - 332 1.6e-07 WDR55
WD40_WDHD1_1st PF24817 183 - 295 2.3e-06 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 207 - 324 6.4e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 214 - 334 1.6e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 214 - 320 1.6e-06 WDR5 beta-propeller domain
UTP15_C PF09384 394 - 537 1.3e-37 UTP15 C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 452, 455
AatII GACGTC 2 cut(s) 264, 474
AccB7I CCANNNNNTGG 1 cut(s) 93
AccBSI CCGCTC 3 cut(s) 237, 285, 928
AccII CGCG 2 cut(s) 452, 887
AccIII TCCGGA 1 cut(s) 1309
AcoI YGGCCR 1 cut(s) 696
AcsI RAATTY 1 cut(s) 1375
AcyI GRCGYC 2 cut(s) 261, 471
AdeI CACNNNGTG 1 cut(s) 586
AfaI GTAC 5 cut(s) 92, 464, 836, 1424, 1442
AfiI CCNNNNNNNGG 4 cut(s) 93, 366, 680, 1437
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 6 cut(s) 106, 756, 909, 1072, 1141, 1255
AhlI ACTAGT 1 cut(s) 1148
AjiI CACGTC 1 cut(s) 661
AjuI GAANNNNNNNTTGG 4 cut(s) 1214, 1246, 1367, 1399
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 9 cut(s) 424, 592, 621, 745, 811, 1048, 1249, 1277, 1393
AluI AGCT 9 cut(s) 424, 592, 621, 745, 811, 1048, 1249, 1277, 1393
Alw21I GWGCWC 2 cut(s) 623, 1395
Alw26I GTCTC 4 cut(s) 271, 439, 476, 699
Ama87I CYCGRG 1 cut(s) 1444
Aor13HI TCCGGA 1 cut(s) 1309
AoxI GGCC 5 cut(s) 292, 322, 696, 1106, 1214
ApeKI GCWGC 1 cut(s) 525
ApoI RAATTY 1 cut(s) 1375
ArsI GACNNNNNNTTYG 2 cut(s) 766, 798
Asp700I GAANNNNTTC 1 cut(s) 875
AspA2I CCTAGG 1 cut(s) 502
AspLEI GCGC 2 cut(s) 525, 925
AspS9I GGNCC 4 cut(s) 96, 375, 537, 1106
AsuC2I CCSGG 1 cut(s) 1087
AsuHPI GGTGA 6 cut(s) 142, 196, 724, 790, 1219, 1237
AsuII TTCGAA 2 cut(s) 980, 1373
AvaI CYCGRG 1 cut(s) 1444
AvaII GGWCC 3 cut(s) 96, 375, 537
AvrII CCTAGG 1 cut(s) 502
BaeGI GKGCMC 1 cut(s) 510
BanII GRGCYC 2 cut(s) 623, 1395
BbsI GAAGAC 1 cut(s) 328
Bbv12I GWGCWC 2 cut(s) 623, 1395
BbvI GCAGC 1 cut(s) 512
BccI CCATC 3 cut(s) 854, 1324, 1450
BceAI ACGGC 3 cut(s) 307, 711, 1171
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 1 cut(s) 1087
BcoDI GTCTC 4 cut(s) 271, 439, 476, 699
BcuI ACTAGT 1 cut(s) 1148
BfaI CTAG 3 cut(s) 503, 963, 1149
BfmI CTRYAG 1 cut(s) 1509
BisI GCNGC 9 cut(s) 183, 186, 306, 309, 442, 526, 529, 682, 926
BlnI CCTAGG 1 cut(s) 502
BlpI GCTNAGC 1 cut(s) 828
BlsI GCNGC 9 cut(s) 184, 187, 307, 310, 443, 527, 530, 683, 927
BmcAI AGTACT 2 cut(s) 92, 464
Bme1390I CCNGG 1 cut(s) 1087
Bme18I GGWCC 3 cut(s) 96, 375, 537
BmeT110I CYCGRG 1 cut(s) 1444
BmgBI CACGTC 1 cut(s) 661
BmgT120I GGNCC 4 cut(s) 96, 375, 537, 1106
BmiI GGNNCC 8 cut(s) 9, 371, 377, 539, 917, 1084, 1107, 1122
BmrFI CCNGG 1 cut(s) 1087
BmrI ACTGGG 2 cut(s) 475, 576
BmuI ACTGGG 2 cut(s) 475, 576
BpiI GAAGAC 1 cut(s) 328
Bpu1102I GCTNAGC 1 cut(s) 828
Bpu14I TTCGAA 2 cut(s) 980, 1373
BpuMI CCSGG 1 cut(s) 1087
BsaAI YACGTR 1 cut(s) 520
BsaHI GRCGYC 2 cut(s) 261, 471
BsaI GGTCTC 2 cut(s) 439, 476
BsaJI CCNNGG 3 cut(s) 502, 1124, 1436
BsaWI WCCGGW 2 cut(s) 372, 1309
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 366, 680, 1437
Bse118I RCCGGY 2 cut(s) 476, 649
Bse1I ACTGG 4 cut(s) 98, 470, 571, 1627
BseAI TCCGGA 1 cut(s) 1309
BseDI CCNNGG 3 cut(s) 502, 1124, 1436
BseGI GGATG 2 cut(s) 604, 865
BseLI CCNNNNNNNGG 4 cut(s) 93, 366, 680, 1437
BseMII CTCAG 3 cut(s) 826, 842, 1182
BseNI ACTGG 4 cut(s) 98, 470, 571, 1627
BseRI GAGGAG 9 cut(s) 229, 259, 287, 717, 1018, 1058, 1133, 1403, 1568
BseSI GKGCMC 1 cut(s) 510
BseX3I CGGCCG 1 cut(s) 696
BseXI GCAGC 1 cut(s) 512
BseYI CCCAGC 1 cut(s) 998
Bsh1236I CGCG 2 cut(s) 452, 887
Bsh1285I CGRYCG 2 cut(s) 456, 699
BshFI GGCC 5 cut(s) 294, 324, 698, 1108, 1216
BsiEI CGRYCG 2 cut(s) 456, 699
BsiHKAI GWGCWC 2 cut(s) 623, 1395
BsiHKCI CYCGRG 1 cut(s) 1444
BslFI GGGAC 3 cut(s) 361, 482, 523
BslI CCNNNNNNNGG 4 cut(s) 93, 366, 680, 1437
BsmAI GTCTC 4 cut(s) 271, 439, 476, 699
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 3 cut(s) 361, 482, 523
BsmI GAATGC 2 cut(s) 1096, 1321
BsnI GGCC 5 cut(s) 294, 324, 698, 1108, 1216
Bso31I GGTCTC 2 cut(s) 439, 476
BsoBI CYCGRG 1 cut(s) 1444
Bsp119I TTCGAA 2 cut(s) 980, 1373
Bsp1286I GDGCHC 3 cut(s) 510, 623, 1395
Bsp13I TCCGGA 1 cut(s) 1309
Bsp143I GATC 1 cut(s) 577
Bsp1720I GCTNAGC 1 cut(s) 828
BspANI GGCC 5 cut(s) 294, 324, 698, 1108, 1216
BspCNI CTCAG 3 cut(s) 825, 841, 1183
BspEI TCCGGA 1 cut(s) 1309
BspFNI CGCG 2 cut(s) 452, 887
BspLI GGNNCC 8 cut(s) 9, 371, 377, 539, 917, 1084, 1107, 1122
BspT104I TTCGAA 2 cut(s) 980, 1373
BspTNI GGTCTC 2 cut(s) 439, 476
BsrBI CCGCTC 3 cut(s) 237, 285, 928
BsrFI RCCGGY 2 cut(s) 476, 649
BsrI ACTGG 4 cut(s) 98, 470, 571, 1627
BssAI RCCGGY 2 cut(s) 476, 649
BssECI CCNNGG 3 cut(s) 502, 1124, 1436
BssMI GATC 1 cut(s) 577
BssNI GRCGYC 2 cut(s) 261, 471
BssT1I CCWWGG 2 cut(s) 502, 1436
Bst4CI ACNGT 3 cut(s) 586, 644, 778
Bst6I CTCTTC 1 cut(s) 816
BstACI GRCGYC 2 cut(s) 261, 471
BstBAI YACGTR 1 cut(s) 520
BstBI TTCGAA 2 cut(s) 980, 1373
BstC8I GCNNGC 2 cut(s) 368, 478
BstDEI CTNAG 4 cut(s) 812, 828, 1191, 1302
BstDSI CCRYGG 1 cut(s) 1124
BstF5I GGATG 2 cut(s) 604, 865
BstFNI CGCG 2 cut(s) 452, 887
BstHHI GCGC 2 cut(s) 525, 925
BstKTI GATC 1 cut(s) 580
BstMAI GTCTC 4 cut(s) 271, 439, 476, 699
BstMBI GATC 1 cut(s) 577
BstMCI CGRYCG 2 cut(s) 456, 699
BstMWI GCNNNNNNNGC 3 cut(s) 291, 393, 1246
BstNSI RCATGY 1 cut(s) 559
BstSCI CCNGG 1 cut(s) 1085
BstSFI CTRYAG 1 cut(s) 1509
BstSLI GKGCMC 1 cut(s) 510
BstUI CGCG 2 cut(s) 452, 887
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 1 cut(s) 328
BstZI CGGCCG 1 cut(s) 696
BsuRI GGCC 5 cut(s) 294, 324, 698, 1108, 1216
BtgI CCRYGG 1 cut(s) 1124
BtgZI GCGATG 2 cut(s) 115, 286
BtrI CACGTC 1 cut(s) 661
BtsCI GGATG 2 cut(s) 604, 865
BtsIMutI CAGTG 4 cut(s) 564, 591, 820, 942
Cac8I GCNNGC 2 cut(s) 368, 478
CfoI GCGC 2 cut(s) 525, 925
Cfr10I RCCGGY 2 cut(s) 476, 649
Cfr13I GGNCC 4 cut(s) 96, 375, 537, 1106
CseI GACGC 1 cut(s) 458
Csp6I GTAC 5 cut(s) 91, 463, 835, 1423, 1441
CviAII CATG 5 cut(s) 556, 868, 931, 1241, 1432
CviQI GTAC 5 cut(s) 91, 463, 835, 1423, 1441
DdeI CTNAG 4 cut(s) 812, 828, 1191, 1302
DpnI GATC 1 cut(s) 579
DpnII GATC 1 cut(s) 577
DraIII CACNNNGTG 1 cut(s) 586
DrdI GACNNNNNNGTC 2 cut(s) 452, 455
DseDI GACNNNNNNGTC 2 cut(s) 452, 455
EaeI YGGCCR 1 cut(s) 696
EagI CGGCCG 1 cut(s) 696
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
EciI GGCGGA 3 cut(s) 20, 262, 1130
Ecl136II GAGCTC 2 cut(s) 621, 1393
EclXI CGGCCG 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 502, 1436
Eco24I GRGCYC 2 cut(s) 623, 1395
Eco31I GGTCTC 2 cut(s) 439, 476
Eco47I GGWCC 3 cut(s) 96, 375, 537
Eco52I CGGCCG 1 cut(s) 696
Eco53kI GAGCTC 2 cut(s) 621, 1393
Eco88I CYCGRG 1 cut(s) 1444
EcoICRI GAGCTC 2 cut(s) 621, 1393
EcoO109I RGGNCCY 1 cut(s) 1106
EcoT14I CCWWGG 2 cut(s) 502, 1436
EcoT38I GRGCYC 2 cut(s) 623, 1395
ErhI CCWWGG 2 cut(s) 502, 1436
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 5 cut(s) 559, 871, 934, 1244, 1435
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FaqI GGGAC 3 cut(s) 361, 482, 523
FatI CATG 5 cut(s) 555, 867, 930, 1240, 1431
FauI CCCGC 2 cut(s) 242, 392
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 9 cut(s) 183, 186, 306, 309, 442, 526, 529, 682, 926
FokI GGATG 2 cut(s) 611, 872
FriOI GRGCYC 2 cut(s) 623, 1395
Fsp4HI GCNGC 9 cut(s) 183, 186, 306, 309, 442, 526, 529, 682, 926
FspBI CTAG 3 cut(s) 503, 963, 1149
GlaI GCGC 2 cut(s) 524, 924
GluI GCNGC 9 cut(s) 183, 186, 306, 309, 442, 526, 529, 682, 926
GsaI CCCAGC 1 cut(s) 1002
HaeIII GGCC 5 cut(s) 294, 324, 698, 1108, 1216
HgaI GACGC 1 cut(s) 458
HhaI GCGC 2 cut(s) 525, 925
Hin1I GRCGYC 2 cut(s) 261, 471
Hin1II CATG 5 cut(s) 559, 871, 934, 1244, 1435
Hin6I GCGC 2 cut(s) 523, 923
HinP1I GCGC 2 cut(s) 523, 923
HincII GTYRAC 1 cut(s) 1464
HindII GTYRAC 1 cut(s) 1464
HinfI GANTC 6 cut(s) 83, 548, 614, 637, 707, 1306
HphI GGTGA 6 cut(s) 142, 196, 724, 790, 1219, 1237
Hpy166II GTNNAC 1 cut(s) 1464
Hpy188I TCNGA 6 cut(s) 289, 1039, 1167, 1180, 1192, 1397
Hpy188III TCNNGA 3 cut(s) 1138, 1310, 1347
Hpy8I GTNNAC 1 cut(s) 1464
Hpy99I CGWCG 7 cut(s) 263, 266, 293, 449, 452, 476, 697
HpyCH4III ACNGT 3 cut(s) 586, 644, 778
HpyCH4IV ACGT 5 cut(s) 261, 471, 519, 660, 1543
HpyCH4V TGCA 2 cut(s) 1417, 1582
HpyF10VI GCNNNNNNNGC 3 cut(s) 291, 393, 1246
HpyF3I CTNAG 4 cut(s) 812, 828, 1191, 1302
HpySE526I ACGT 5 cut(s) 261, 471, 519, 660, 1543
Hsp92I GRCGYC 2 cut(s) 261, 471
Hsp92II CATG 5 cut(s) 559, 871, 934, 1244, 1435
HspAI GCGC 2 cut(s) 523, 923
Kpn2I TCCGGA 1 cut(s) 1309
KroI GCCGGC 1 cut(s) 476
KroNI GCCGGC 1 cut(s) 478
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 1 cut(s) 577
LmnI GCTCC 9 cut(s) 242, 290, 375, 429, 626, 1045, 1120, 1237, 1390
Lsp1109I GCAGC 1 cut(s) 512
MaeI CTAG 3 cut(s) 503, 963, 1149
MaeII ACGT 5 cut(s) 261, 471, 519, 660, 1543
MaeIII GTNAC 4 cut(s) 625, 778, 863, 898
MalI GATC 1 cut(s) 579
MbiI CCGCTC 3 cut(s) 237, 285, 928
MboI GATC 1 cut(s) 577
MboII GAAGA 8 cut(s) 328, 727, 833, 1031, 1084, 1231, 1267, 1346
MhlI GDGCHC 3 cut(s) 510, 623, 1395
MluCI AATT 4 cut(s) 227, 687, 1337, 1375
MlyI GAGTC 1 cut(s) 701
MmeI TCCRAC 3 cut(s) 267, 312, 576
MreI CGCCGGCG 1 cut(s) 476
MroI TCCGGA 1 cut(s) 1309
MroNI GCCGGC 1 cut(s) 476
MroXI GAANNNNTTC 1 cut(s) 875
MseI TTAA 2 cut(s) 686, 1539
MslI CAYNNNNRTG 1 cut(s) 935
MspR9I CCNGG 1 cut(s) 1087
Mva1269I GAATGC 2 cut(s) 1096, 1321
MvnI CGCG 2 cut(s) 452, 887
MwoI GCNNNNNNNGC 3 cut(s) 291, 393, 1246
NaeI GCCGGC 1 cut(s) 478
NciI CCSGG 1 cut(s) 1087
NdeII GATC 1 cut(s) 577
NgoMIV GCCGGC 1 cut(s) 476
NlaIII CATG 5 cut(s) 559, 871, 934, 1244, 1435
NlaIV GGNNCC 8 cut(s) 9, 371, 377, 539, 917, 1084, 1107, 1122
NmuCI GTSAC 2 cut(s) 625, 778
NspI RCATGY 1 cut(s) 559
NspV TTCGAA 2 cut(s) 980, 1373
PaeR7I CTCGAG 1 cut(s) 1444
PciI ACATGT 1 cut(s) 555
PcsI WCGNNNNNNNCGW 4 cut(s) 309, 453, 477, 782
PctI GAATGC 2 cut(s) 1096, 1321
PdiI GCCGGC 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 875
PfeI GAWTC 5 cut(s) 83, 548, 614, 637, 1306
PflFI GACNNNGTC 2 cut(s) 263, 452
PflMI CCANNNNNTGG 1 cut(s) 93
PfoI TCCNGGA 1 cut(s) 1085
PkrI GCNGC 9 cut(s) 184, 187, 307, 310, 443, 527, 530, 683, 927
PleI GAGTC 1 cut(s) 701
PpsI GAGTC 1 cut(s) 701
Ppu21I YACGTR 1 cut(s) 520
PscI ACATGT 1 cut(s) 555
Psp124BI GAGCTC 2 cut(s) 623, 1395
PspFI CCCAGC 1 cut(s) 998
PspN4I GGNNCC 8 cut(s) 9, 371, 377, 539, 917, 1084, 1107, 1122
PspPI GGNCC 4 cut(s) 96, 375, 537, 1106
PspXI VCTCGAGB 1 cut(s) 1444
PsyI GACNNNGTC 2 cut(s) 263, 452
RsaI GTAC 5 cut(s) 92, 464, 836, 1424, 1442
RsaNI GTAC 5 cut(s) 91, 463, 835, 1423, 1441
RseI CAYNNNNRTG 1 cut(s) 935
SacI GAGCTC 2 cut(s) 623, 1395
SaqAI TTAA 2 cut(s) 686, 1539
SatI GCNGC 9 cut(s) 183, 186, 306, 309, 442, 526, 529, 682, 926
Sau3AI GATC 1 cut(s) 577
Sau96I GGNCC 4 cut(s) 96, 375, 537, 1106
ScaI AGTACT 2 cut(s) 92, 464
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 1 cut(s) 1087
SduI GDGCHC 3 cut(s) 510, 623, 1395
SfcI CTRYAG 1 cut(s) 1509
Sfr274I CTCGAG 1 cut(s) 1444
SfuI TTCGAA 2 cut(s) 980, 1373
SgrAI CRCCGGYG 1 cut(s) 476
SinI GGWCC 3 cut(s) 96, 375, 537
SlaI CTCGAG 1 cut(s) 1444
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 1444
SmoI CTYRAG 1 cut(s) 1444
SpeI ACTAGT 1 cut(s) 1148
Sse9I AATT 4 cut(s) 227, 687, 1337, 1375
SspMI CTAG 3 cut(s) 503, 963, 1149
SstI GAGCTC 2 cut(s) 623, 1395
StyD4I CCNGG 1 cut(s) 1085
StyI CCWWGG 2 cut(s) 502, 1436
TaaI ACNGT 3 cut(s) 586, 644, 778
TaiI ACGT 5 cut(s) 264, 474, 522, 663, 1546
TasI AATT 4 cut(s) 227, 687, 1337, 1375
TatI WGTACW 3 cut(s) 90, 462, 834
TauI GCSGC 8 cut(s) 185, 188, 308, 311, 444, 531, 684, 928
TfiI GAWTC 5 cut(s) 83, 548, 614, 637, 1306
Tru1I TTAA 2 cut(s) 686, 1539
Tru9I TTAA 2 cut(s) 686, 1539
TscAI CASTG 4 cut(s) 571, 591, 820, 942
TseFI GTSAC 2 cut(s) 625, 778
TseI GCWGC 1 cut(s) 525
Tsp45I GTSAC 2 cut(s) 625, 778
TspDTI ATGAA 4 cut(s) 550, 884, 908, 1074
TspGWI ACGGA 3 cut(s) 613, 644, 1141
TspRI CASTG 4 cut(s) 571, 591, 820, 942
Tth111I GACNNNGTC 2 cut(s) 263, 452
Van91I CCANNNNNTGG 1 cut(s) 93
VpaK11BI GGWCC 3 cut(s) 96, 375, 537
XapI RAATTY 1 cut(s) 1375
XceI RCATGY 1 cut(s) 559
XhoI CTCGAG 1 cut(s) 1444
XmaJI CCTAGG 1 cut(s) 502
XmnI GAANNNNTTC 1 cut(s) 875
XspI CTAG 3 cut(s) 503, 963, 1149
ZraI GACGTC 2 cut(s) 262, 472
ZrmI AGTACT 2 cut(s) 92, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.