MD08G1203800.v1.1

Pyruvate phosphate dikinase, PEP/pyruvate binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004913
Physical Location & Seq
Reverse (-)
26350414 .. 26364203
13790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1203800.v1.1.491

Sequence Viewer

Length: 3930 bp
ATGGCCGCCGCCGGTAACCACCCTTCCGGCTCATCACAGGCGCCTGCTCCTCCTCCTCCTCGTCTCCACCAGTTTGATCTCATTGACCGCATGCAACTTCAGATTAATGTCTCGGGTTCTTCGAAGGGGCGCAATGCAAGACTTGAATTTCAGCTCAGCAACTGCCCCAGAACTTGGGTTCTTCACTGGGGTTGCATTCTCCGTGGAAATAAGAACTGGTTCGTTCCCGCTGATCGGTCTTCGGGTTCACAAGCTTACAAGCAAGGCGCATTGCAGACACAATTCACCAAGAAAGGAGAATTGTATTTGTTAACCATCGAGTTGCGGGACCCCAACGTACATGCTATTGAATTTGTACTTAAAGATGGCAGTCGCGAGAGATGGTTGAAAATGAATCACGGGAACTTCCGTATAGAAATCCCTGAGACTGATCCAAGTACTTTAAGTCCCCCAATACCAAAAGAGCTGATTGAACAGAAAAGCAAGGGCAGGCCCATGAGCTCGCCACAACAAGAAAAGGAAGATTACAAGGATACTTTAATAGGGCTACAAAGTCAGATATCAAAAGGAATATCCTTTAATGAGTTGCAGCATAGTTTTTCAACCTCCAGTCCTAGAAGAATGGTTGATAATGGAGAGAAACTGAGGAGTGGGATGCCATACTCGTACAAAAGGAAACATAATGTTGAGCAGTGGTTGCAAAAGCATTCAACAGCACCTGCCAAAAACGCTAGTATGCCAAATTCAGCTCTCATGGATCTTGTAGAAAAATCTATGGGAGGAGACAATGTGGTCTCACGGATAACTTATCAAATTGGCAACTATGACATAGTGGTCCTTTCAAAAATGGATAGGGGTGACTTTCACATATTGGTTGCTACAAATATGAGAGGTGATACAGTTCTTCACTGGGGTGTTTCCAAATGTTCCCCTGGGGATTGGTTGGCTCCTCCACCAGAGATATTGCCTAAAAAATCAAATTTAGTTCCTGGAGCATGTCAGACTTATTTTACAGAAATATCAACTGGAAAGGGTTCCTTTCAGATTGTAGATATTAATCTTCAGCAAAGCAATTTGGTGGGTATCCAATTTGTGATATGGTCTGCTGGGTCTTCTTGGATAAAGAACAATGGAGCAAACTTCTTTGTAGGCGTGAGTCGCGTGACACCAGTGACTTCTACTGCAAAGGCTAGAGGGAATGGTGATGGAATCTGCAAATGGTTACTTGATGAAATATCTCGAAGAGAGAAGGAGGCCGAGAGATCGTTGATGCACAGATTCAACATTGCAACGGAACTCACAGAACGCTGCAAAAGTGAAGGGGAGTTCGGGCTTGTTGGTATATTGGTATGGTTGAGGTTCATGTCATGCAGGCATCTAACATGGAACAAGAACTACAATGTGAAACCTCGTGAAATTAGTGAAGCTCAGGATAGGTTTACCAATCTGCTACAAAGAAAATATTTAAACCAGCCTAATGATTGGGAAATTGTGAGATTACTAATGACACATGTTGGTCGTGGAGGTCAGGGAGATGTTGGACAGAGAATTAGAGATGAAATACTTGTGATTCAGAGAAATAATGACTGTAAGGGTGGCATGATGGAGGAATGGCACCAAAAGTTGCACAATAATAGTAGCCCAGATGATGTGATAATTTGTGAGGCGCTCTTAAATTTCATCAAGTCTAGTTTTAGAGTTGATGTTTATTGGAGAACATTAAATGCCAATGGTCTCACAAAAGAGAAACTTGCAAGTTATGACCGCCCAATTGTGTCAGAGCCGCATTTCAGGGCTGATACTAAGGAGGGACTCATCCGTGACCTCACAGCATACTTGAAGACATTAAAGGCTGTGCATTCAGGTGCTGACCTTGAATCAGCTATTGAAGTTTTGGTTCCCTCAAATGAGAGTCGTAATTTCTCAAGCATGGTTGGAGTTAATTATGTTTGTGATTTGTCACCTAGGTTGCAGGAATGCCTGAACTTTGTCAAAGCACACGTTGGTGATGAAGATATTGTTCCACTAATGGAGAAGTTACTAGAATCTCGAATTGAGCTACGCCCTGTTCTAATCACCAACCACAGAAGATTGAAAGATATACTCTTCCTTGATATTGCTTTGGATTCAGCTGTCAGAACAACCATGGAGAGGGGACTGAAAAATCTGAACTCTGGACACCCACCAGAGATTATGTTCTTCATTTCCTTGGTGCTCGAAAATTTATGTCTGTCAACGGTTGACAATGAAGACCTCATTTACTGTACCAAGGACTGGTACCGTACCTGCGAATTGTACAAACCCAATGATGGTCAGTGGGCTTTACAAACAAAGGCTATTCTTGATAGATTACAATTAGTACTTGCTGAGAGGTCTCAGTGTTACCAGAAGAAGATCCAGCCCAGTGCAAAATATCTCGGCAATTTGCTTGGTGTTCAGAAATCGCCGATTGATACTTTCTCTGAGGAGTTAATAAGGGCTGGGTCAGCAGCTATTCTGTCTACCCTCATTAATCGCTTTAACCCCATTCTTAGAAAAGCTGCAAACTTGGGCAGTTGGCAGGTTATCAGCCCAGTAGAAGTGTCAGGATTCTTACATTCTGTTAGTGAGCTCATTAGTATTCAGAACAAAGTCTATAAAAAGCCAACCGTTTTAATTGCAACTAGAGTAACGGGAGAAGAGGAGATTCCAGATGGAGTTGTTGCTGTTCTGACACCTGATTTACCTGATGTTTTATCTCATGTCTCTATAAGAGCAAGAAATGACAAGGTATGCTTTGCCACATGCTTTGATCCGAATATTATCAGAGATCTCAAGTTGAAGGAAGGCAAACCAATATCAATACAAGTGAAGTCGGCAAATATTATTATCAGTGACATCAGTAGCTCCAATCTTTCTTACAAGTCATTTGCTACTCAGTCCTCCAATCATCCAGGATTAACGCTAAAAAAGAAGGCCTTCTGTGGTAAATATGCTGTCTCAATTCAGGAGTTCACTAGTGAGGTGGTTGGTGCAAAATCTTTCAATATAAAAGTTTTGAGAGAAAGGGTGCCGACATGGATTAAAATTCCAATGTCAGTAGCCATACCATTTGGAGCCTTTGAGAAAGTCCTACTGGAAGATGTTAATAAGGATATAGCATATAAGATTTCCTCATTTTACAAATGTGTAAAGAGCGGAGACCTCTCAAAACTTCAAGCAATACGAGACACTATTCTACAAATGAATGCTCCTATTTCATTGATCCATGAGCTTAAGAGCAAAATGAAAAGTTCAGGAATACCTTGGCCTGGTGATGAAGGTGGCGAGAGGTGGAATCGTGCTTGGCAAGCAATAAAGAAGGTTTGGGCTTCAAAGTGGAATGAAAGAGCATTTCTTAGTTGTAGGAAAGCTAACTTAGATCATGAAAATATATGCATGGCTGTACTGATTCAAGAAATTATTTGCGCTGATTATGCTTTTGTAATTCACACGAAGAATCCCCTATCAGGGGATACCTCAGAGATTTATACAGAGATAGTTAAGGGCTTGGGAGAGACTTTGGTGGGTGCATATCCCGGACGTGCCTTGAGCTTCATTACCAAAAAATCAAATCTAAATTCTCCTGTTGTTACTGGTTATCCAAGCAAGCCAATAGGATTATATAGTAAGCAGTCTATCATATTCAGATCAGACTCAAACGCCGAGGACCTGGAAGGATATGCAGGTGCTGGGCTTTATGACAGTGTGATCATGGATAAAGAGGAGAAGATTGTATTGGATTACTCGAGAGATCAGTTGATTATTGACAGACCCTTCCAAGTCTCAGTCTTGTCGAGAATTGCAGAGGCGGGAAAGATAGTAGAAGGCATTTACGGTCATCCTCAGGACATTGAAGGGGTGGTAAAAGATGGAGTGATCTATGTGGTTCAGTCAAGGCCACAAATCTAG

Protein Analysis

1310

Amino Acids

146.91

Weight (kDa)

8.84

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_N_CWD1 PF23166 23 - 140 1.1e-34 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
DUF7067 PF23229 161 - 201 2.9e-10 Alpha-glucan water dikinase, DUF7067
Ig_N_CWD1 PF23166 281 - 383 4.2e-20 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
GWD1_pHisD PF22973 852 - 965 1.2e-50 Alpha-glucan water dikinase, phosphohistidine-like domain
PPDK_N PF01326 1108 - 1308 1.2e-19 Pyruvate phosphate dikinase, AMP/ATP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 727, 3695
AasI GACNNNNNNGTC 2 cut(s) 791, 833
Acc36I ACCTGC 4 cut(s) 727, 2294, 2551, 3695
Acc65I GGTACC 1 cut(s) 2277
AccB1I GGYRCC 4 cut(s) 40, 1614, 2277, 3049
AccB7I CCANNNNNTGG 2 cut(s) 174, 2274
AccBSI CCGCTC 1 cut(s) 3177
AccI GTMKAC 1 cut(s) 2501
AccII CGCG 2 cut(s) 375, 1161
AciI CCGC 9 cut(s) 6, 9, 88, 228, 325, 1765, 1784, 3177, 3830
AclWI GGATC 5 cut(s) 425, 765, 2389, 2786, 3238
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 8 cut(s) 146, 350, 742, 979, 1675, 2221, 3066, 3598
AcuI CTGAAG 2 cut(s) 83, 1046
AcyI GRCGYC 1 cut(s) 41
AfiI CCNNNNNNNGG 9 cut(s) 174, 234, 2151, 2274, 2309, 3290, 3488, 3489, 3490
AflII CTTAAG 1 cut(s) 3254
AflIII ACRYGT 2 cut(s) 1510, 1999
AhlI ACTAGT 1 cut(s) 2996
AjiI CACGTC 1 cut(s) 3563
AjnI CCWGG 5 cut(s) 931, 988, 2932, 3289, 3690
AjuI GAANNNNNNNTTGG 2 cut(s) 3740, 3772
AleI CACNNNNGTG 2 cut(s) 912, 2004
Alw21I GWGCWC 3 cut(s) 503, 2217, 2613
AlwI GGATC 5 cut(s) 425, 765, 2389, 2786, 3238
AlwNI CAGNNNCTG 4 cut(s) 162, 719, 1868, 3710
Ama87I CYCGRG 2 cut(s) 112, 3766
AoxI GGCC 6 cut(s) 3, 491, 1254, 2955, 3287, 3917
ApeKI GCWGC 4 cut(s) 589, 1308, 2489, 2540
ApoI RAATTY 8 cut(s) 146, 350, 742, 979, 1675, 2221, 3066, 3598
AseI ATTAAT 3 cut(s) 105, 1056, 2511
Asp700I GAANNNNTTC 3 cut(s) 218, 1033, 2957
Asp718I GGTACC 1 cut(s) 2277
AspA2I CCTAGG 1 cut(s) 1964
AspLEI GCGC 5 cut(s) 43, 132, 269, 1669, 3449
AspS9I GGNCC 4 cut(s) 328, 492, 835, 3688
AsuC2I CCSGG 1 cut(s) 3558
AsuHPI GGTGA 8 cut(s) 277, 869, 905, 1214, 1953, 2018, 2068, 3305
AsuII TTCGAA 1 cut(s) 122
AvaI CYCGRG 2 cut(s) 112, 3766
AvaII GGWCC 3 cut(s) 328, 835, 3688
AvrII CCTAGG 1 cut(s) 1964
AxyI CCTNAGG 1 cut(s) 3864
BanI GGYRCC 4 cut(s) 40, 1614, 2277, 3049
BanII GRGCYC 2 cut(s) 503, 2613
BarI GAAGNNNNNNTAC 2 cut(s) 7, 39
BauI CACGAG 1 cut(s) 1410
BbsI GAAGAC 4 cut(s) 231, 1104, 1847, 2256
Bbv12I GWGCWC 3 cut(s) 503, 2217, 2613
BbvI GCAGC 4 cut(s) 601, 1295, 2501, 2527
BccI CCATC 8 cut(s) 323, 359, 375, 1199, 1597, 2303, 2687, 3884
BciT130I CCWGG 5 cut(s) 933, 990, 2934, 3291, 3692
BciVI GTATCC 3 cut(s) 526, 1094, 3487
BclI TGATCA 1 cut(s) 3729
BcnI CCSGG 1 cut(s) 3558
BcuI ACTAGT 1 cut(s) 2996
BfaI CTAG 9 cut(s) 615, 732, 1191, 1689, 1965, 2042, 2664, 2997, 3928
BfoI RGCGCY 2 cut(s) 44, 1670
BfrI CTTAAG 1 cut(s) 3254
BfuAI ACCTGC 4 cut(s) 727, 2294, 2551, 3695
BfuI GTATCC 3 cut(s) 526, 1094, 3487
BglII AGATCT 1 cut(s) 2809
BisI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
BlnI CCTAGG 1 cut(s) 1964
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 7 cut(s) 7, 10, 591, 1310, 1785, 2491, 2542
BmcAI AGTACT 2 cut(s) 439, 2361
Bme1390I CCNGG 6 cut(s) 933, 990, 2934, 3291, 3558, 3692
Bme18I GGWCC 3 cut(s) 328, 835, 3688
BmeT110I CYCGRG 2 cut(s) 112, 3766
BmgBI CACGTC 1 cut(s) 3563
BmgT120I GGNCC 4 cut(s) 328, 492, 835, 3688
BmrFI CCNGG 6 cut(s) 933, 990, 2934, 3291, 3558, 3692
BmrI ACTGGG 4 cut(s) 196, 919, 2397, 2567
BmsI GCATC 3 cut(s) 645, 1260, 1384
BmuI ACTGGG 4 cut(s) 196, 919, 2397, 2567
BpiI GAAGAC 4 cut(s) 231, 1104, 1847, 2256
BpmI CTGGAG 2 cut(s) 592, 1011
Bpu10I CCTNAGC 1 cut(s) 1428
Bpu1102I GCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 122
BpuEI CTTGAG 3 cut(s) 1909, 2798, 3589
BpuMI CCSGG 1 cut(s) 3558
BsaBI GATNNNNATC 1 cut(s) 1056
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 4 cut(s) 799, 1739, 2379, 3174
BsaJI CCNNGG 9 cut(s) 202, 931, 932, 1964, 2145, 2208, 2268, 3284, 3684
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bsc4I CCNNNNNNNGG 9 cut(s) 174, 234, 2151, 2274, 2309, 3290, 3488, 3489, 3490
Bse118I RCCGGY 1 cut(s) 11
Bse21I CCTNAGG 1 cut(s) 3864
Bse3DI GCAATG 3 cut(s) 139, 269, 1284
Bse8I GATNNNNATC 1 cut(s) 1056
BseBI CCWGG 5 cut(s) 933, 990, 2934, 3291, 3692
BseDI CCNNGG 9 cut(s) 202, 931, 932, 1964, 2145, 2208, 2268, 3284, 3684
BseGI GGATG 4 cut(s) 660, 1815, 2929, 3859
BseJI GATNNNNATC 1 cut(s) 1056
BseLI CCNNNNNNNGG 9 cut(s) 174, 234, 2151, 2274, 2309, 3290, 3488, 3489, 3490
BseMI GCAATG 3 cut(s) 139, 269, 1284
BseXI GCAGC 4 cut(s) 601, 1295, 2501, 2527
BseYI CCCAGC 3 cut(s) 1106, 2480, 3710
Bsh1236I CGCG 2 cut(s) 375, 1161
BshFI GGCC 6 cut(s) 5, 493, 1256, 2957, 3289, 3919
BshNI GGYRCC 4 cut(s) 40, 1614, 2277, 3049
BsiHKAI GWGCWC 3 cut(s) 503, 2217, 2613
BsiHKCI CYCGRG 2 cut(s) 112, 3766
BsiSI CCGG 3 cut(s) 12, 27, 3558
BslFI GGGAC 4 cut(s) 341, 432, 1824, 2169
BslI CCNNNNNNNGG 9 cut(s) 174, 234, 2151, 2274, 2309, 3290, 3488, 3489, 3490
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 4 cut(s) 341, 432, 1824, 2169
BsmI GAATGC 5 cut(s) 195, 706, 1858, 1982, 3232
BsnI GGCC 6 cut(s) 5, 493, 1256, 2957, 3289, 3919
Bso31I GGTCTC 4 cut(s) 799, 1739, 2379, 3174
BsoBI CYCGRG 2 cut(s) 112, 3766
Bsp119I TTCGAA 1 cut(s) 122
Bsp1286I GDGCHC 3 cut(s) 503, 2217, 2613
Bsp1407I TGTACA 1 cut(s) 2295
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp19I CCATGG 1 cut(s) 2145
Bsp68I TCGCGA 1 cut(s) 375
BspACI CCGC 9 cut(s) 6, 9, 88, 228, 325, 1765, 1784, 3177, 3830
BspANI GGCC 6 cut(s) 5, 493, 1256, 2957, 3289, 3919
BspFNI CGCG 2 cut(s) 375, 1161
BspHI TCATGA 1 cut(s) 3403
BspMI ACCTGC 4 cut(s) 727, 2294, 2551, 3695
BspPI GGATC 5 cut(s) 425, 765, 2389, 2786, 3238
BspT104I TTCGAA 1 cut(s) 122
BspT107I GGYRCC 4 cut(s) 40, 1614, 2277, 3049
BspTI CTTAAG 1 cut(s) 3254
BspTNI GGTCTC 4 cut(s) 799, 1739, 2379, 3174
BsrBI CCGCTC 1 cut(s) 3177
BsrDI GCAATG 3 cut(s) 139, 269, 1284
BsrFI RCCGGY 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 2295
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 9 cut(s) 202, 931, 932, 1964, 2145, 2208, 2268, 3284, 3684
BssNI GRCGYC 1 cut(s) 41
BssSI CACGAG 1 cut(s) 1410
BssT1I CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3284
Bst2BI CACGAG 1 cut(s) 1410
Bst2UI CCWGG 5 cut(s) 933, 990, 2934, 3291, 3692
Bst4CI ACNGT 8 cut(s) 901, 1589, 2239, 2264, 2282, 2650, 3725, 3857
Bst6I CTCTTC 3 cut(s) 1237, 2111, 2673
BstACI GRCGYC 1 cut(s) 41
BstAFI CTTAAG 1 cut(s) 3254
BstAPI GCANNNNNTGC 1 cut(s) 697
BstAUI TGTACA 1 cut(s) 2295
BstBI TTCGAA 1 cut(s) 122
BstC8I GCNNGC 7 cut(s) 45, 92, 491, 503, 1373, 3330, 3629
BstDSI CCRYGG 2 cut(s) 202, 2145
BstEII GGTNACC 1 cut(s) 14
BstF5I GGATG 4 cut(s) 660, 1815, 2929, 3859
BstFNI CGCG 2 cut(s) 375, 1161
BstH2I RGCGCY 2 cut(s) 44, 1670
BstHHI GCGC 5 cut(s) 43, 132, 269, 1669, 3449
BstMWI GCNNNNNNNGC 6 cut(s) 697, 728, 1158, 2486, 3329, 3455
BstNI CCWGG 5 cut(s) 933, 990, 2934, 3291, 3692
BstNSI RCATGY 5 cut(s) 94, 344, 999, 1514, 2787
BstPI GGTNACC 1 cut(s) 14
BstSCI CCNGG 6 cut(s) 931, 988, 2932, 3289, 3556, 3690
BstUI CGCG 2 cut(s) 375, 1161
BstV1I GCAGC 4 cut(s) 601, 1295, 2501, 2527
BstV2I GAAGAC 4 cut(s) 231, 1104, 1847, 2256
BstX2I RGATCY 3 cut(s) 757, 2394, 2809
BstYI RGATCY 3 cut(s) 757, 2394, 2809
Bsu36I CCTNAGG 1 cut(s) 3864
BsuI GTATCC 3 cut(s) 526, 1094, 3487
BsuRI GGCC 6 cut(s) 5, 493, 1256, 2957, 3289, 3919
BtgI CCRYGG 2 cut(s) 202, 2145
BtrI CACGTC 1 cut(s) 3563
BtsCI GGATG 4 cut(s) 660, 1815, 2929, 3859
BtsI GCAGTG 1 cut(s) 698
BtsIMutI CAGTG 9 cut(s) 184, 698, 907, 1176, 2321, 2384, 2410, 2878, 3730
BtuMI TCGCGA 1 cut(s) 375
BveI ACCTGC 4 cut(s) 727, 2294, 2551, 3695
Cac8I GCNNGC 7 cut(s) 45, 92, 491, 503, 1373, 3330, 3629
CaiI CAGNNNCTG 4 cut(s) 162, 719, 1868, 3710
CciI TCATGA 1 cut(s) 3403
CfoI GCGC 5 cut(s) 43, 132, 269, 1669, 3449
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 4 cut(s) 328, 492, 835, 3688
CspCI CAANNNNNGTGG 2 cut(s) 2770, 2805
DinI GGCGCC 1 cut(s) 42
DraI TTTAAA 1 cut(s) 1467
DrdI GACNNNNNNGTC 2 cut(s) 791, 833
DseDI GACNNNNNNGTC 2 cut(s) 791, 833
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 1237, 2111, 2673
EarI CTCTTC 3 cut(s) 1237, 2111, 2673
Ecl136II GAGCTC 2 cut(s) 501, 2611
Eco130I CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3284
Eco147I AGGCCT 1 cut(s) 2957
Eco24I GRGCYC 2 cut(s) 503, 2613
Eco31I GGTCTC 4 cut(s) 799, 1739, 2379, 3174
Eco32I GATATC 1 cut(s) 561
Eco47I GGWCC 3 cut(s) 328, 835, 3688
Eco53kI GAGCTC 2 cut(s) 501, 2611
Eco57I CTGAAG 2 cut(s) 83, 1046
Eco81I CCTNAGG 1 cut(s) 3864
Eco88I CYCGRG 2 cut(s) 112, 3766
Eco91I GGTNACC 1 cut(s) 14
EcoICRI GAGCTC 2 cut(s) 501, 2611
EcoO109I RGGNCCY 2 cut(s) 328, 3688
EcoO65I GGTNACC 1 cut(s) 14
EcoRII CCWGG 5 cut(s) 931, 988, 2932, 3289, 3690
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3284
EcoT22I ATGCAT 1 cut(s) 3419
EcoT38I GRGCYC 2 cut(s) 503, 2613
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3284
Esp3I CGTCTC 1 cut(s) 68
FalI AAGNNNNNCTT 8 cut(s) 1022, 1054, 1734, 1766, 2759, 2791, 2942, 2974
FaqI GGGAC 4 cut(s) 341, 432, 1824, 2169
FauI CCCGC 3 cut(s) 235, 318, 3823
FbaI TGATCA 1 cut(s) 3729
FblI GTMKAC 1 cut(s) 2501
Fnu4HI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
FokI GGATG 4 cut(s) 667, 1802, 2916, 3846
FriOI GRGCYC 2 cut(s) 503, 2613
Fsp4HI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
FspBI CTAG 9 cut(s) 615, 732, 1191, 1689, 1965, 2042, 2664, 2997, 3928
GlaI GCGC 5 cut(s) 42, 131, 268, 1668, 3448
GluI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
GsaI CCCAGC 3 cut(s) 1110, 2484, 3714
GsuI CTGGAG 2 cut(s) 592, 1011
HaeII RGCGCY 2 cut(s) 44, 1670
HaeIII GGCC 6 cut(s) 5, 493, 1256, 2957, 3289, 3919
HapII CCGG 3 cut(s) 12, 27, 3558
HhaI GCGC 5 cut(s) 43, 132, 269, 1669, 3449
Hin1I GRCGYC 1 cut(s) 41
Hin6I GCGC 5 cut(s) 41, 130, 267, 1667, 3447
HinP1I GCGC 5 cut(s) 41, 130, 267, 1667, 3447
HincII GTYRAC 3 cut(s) 312, 2235, 2242
HindII GTYRAC 3 cut(s) 312, 2235, 2242
HindIII AAGCTT 1 cut(s) 252
HpaI GTTAAC 1 cut(s) 312
HpaII CCGG 3 cut(s) 12, 27, 3558
HphI GGTGA 8 cut(s) 277, 869, 905, 1214, 1953, 2018, 2068, 3305
Hpy166II GTNNAC 7 cut(s) 248, 312, 1440, 2235, 2242, 2502, 2994
Hpy8I GTNNAC 7 cut(s) 248, 312, 1440, 2235, 2242, 2502, 2994
HpyCH4III ACNGT 8 cut(s) 901, 1589, 2239, 2264, 2282, 2650, 3725, 3857
HpyCH4IV ACGT 3 cut(s) 336, 2001, 3562
HpyF10VI GCNNNNNNNGC 6 cut(s) 697, 728, 1158, 2486, 3329, 3455
HpySE526I ACGT 3 cut(s) 336, 2001, 3562
Hsp92I GRCGYC 1 cut(s) 41
HspAI GCGC 5 cut(s) 41, 130, 267, 1667, 3447
KasI GGCGCC 1 cut(s) 40
KflI GGGWCCC 1 cut(s) 328
KpnI GGTACC 1 cut(s) 2281
Ksp22I TGATCA 1 cut(s) 3729
KspAI GTTAAC 1 cut(s) 312
LmnI GCTCC 7 cut(s) 52, 952, 992, 1133, 2891, 3095, 3235
Lsp1109I GCAGC 4 cut(s) 601, 1295, 2501, 2527
LweI GCATC 3 cut(s) 645, 1260, 1384
MaeI CTAG 9 cut(s) 615, 732, 1191, 1689, 1965, 2042, 2664, 2997, 3928
MaeII ACGT 3 cut(s) 336, 2001, 3562
MbiI CCGCTC 1 cut(s) 3177
MfeI CAATTG 1 cut(s) 1770
MflI RGATCY 3 cut(s) 757, 2394, 2809
MhlI GDGCHC 3 cut(s) 503, 2217, 2613
Mly113I GGCGCC 1 cut(s) 41
MlyI GAGTC 4 cut(s) 1165, 1806, 1921, 3668
MmeI TCCRAC 2 cut(s) 1519, 1915
Mph1103I ATGCAT 1 cut(s) 3419
MroXI GAANNNNTTC 3 cut(s) 218, 1033, 2957
MslI CAYNNNNRTG 3 cut(s) 912, 1863, 2004
MspA1I CMGCKG 2 cut(s) 230, 2132
MspCI CTTAAG 1 cut(s) 3254
MspI CCGG 3 cut(s) 12, 27, 3558
MspR9I CCNGG 6 cut(s) 933, 990, 2934, 3291, 3558, 3692
MunI CAATTG 1 cut(s) 1770
Mva1269I GAATGC 5 cut(s) 195, 706, 1858, 1982, 3232
MvaI CCWGG 5 cut(s) 933, 990, 2934, 3291, 3692
MvnI CGCG 2 cut(s) 375, 1161
MwoI GCNNNNNNNGC 6 cut(s) 697, 728, 1158, 2486, 3329, 3455
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 1 cut(s) 3558
NcoI CCATGG 1 cut(s) 2145
NmeAIII GCCGAG 3 cut(s) 1282, 2397, 3709
NmuCI GTSAC 6 cut(s) 857, 1162, 1171, 1820, 1959, 2873
NruI TCGCGA 1 cut(s) 375
NsiI ATGCAT 1 cut(s) 3419
NspI RCATGY 5 cut(s) 94, 344, 999, 1514, 2787
NspV TTCGAA 1 cut(s) 122
OliI CACNNNNGTG 2 cut(s) 912, 2004
PaeI GCATGC 1 cut(s) 94
PaeR7I CTCGAG 1 cut(s) 3766
PagI TCATGA 1 cut(s) 3403
PaqCI CACCTGC 2 cut(s) 727, 3695
PasI CCCWGGG 1 cut(s) 932
PceI AGGCCT 1 cut(s) 2957
PciI ACATGT 1 cut(s) 1510
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PctI GAATGC 5 cut(s) 195, 706, 1858, 1982, 3232
PdmI GAANNNNTTC 3 cut(s) 218, 1033, 2957
PflMI CCANNNNNTGG 2 cut(s) 174, 2274
PfoI TCCNGGA 3 cut(s) 988, 2932, 3556
PkrI GCNGC 7 cut(s) 7, 10, 591, 1310, 1785, 2491, 2542
PleI GAGTC 4 cut(s) 1164, 1806, 1920, 3668
PluTI GGCGCC 1 cut(s) 44
PpsI GAGTC 4 cut(s) 1164, 1806, 1920, 3668
PpuMI RGGWCCY 2 cut(s) 328, 3688
PscI ACATGT 1 cut(s) 1510
PshBI ATTAAT 3 cut(s) 105, 1056, 2511
Psp124BI GAGCTC 2 cut(s) 503, 2613
Psp5II RGGWCCY 2 cut(s) 328, 3688
Psp6I CCWGG 5 cut(s) 931, 988, 2932, 3289, 3690
PspEI GGTNACC 1 cut(s) 14
PspFI CCCAGC 3 cut(s) 1106, 2480, 3710
PspGI CCWGG 5 cut(s) 931, 988, 2932, 3289, 3690
PspPI GGNCC 4 cut(s) 328, 492, 835, 3688
PspPPI RGGWCCY 2 cut(s) 328, 3688
PsrI GAACNNNNNNTAC 2 cut(s) 1379, 1411
PstNI CAGNNNCTG 4 cut(s) 162, 719, 1868, 3710
PsuI RGATCY 3 cut(s) 757, 2394, 2809
PvuII CAGCTG 1 cut(s) 2132
RruI TCGCGA 1 cut(s) 375
RseI CAYNNNNRTG 3 cut(s) 912, 1863, 2004
SacI GAGCTC 2 cut(s) 503, 2613
SatI GCNGC 7 cut(s) 6, 9, 590, 1309, 1784, 2490, 2541
Sau96I GGNCC 4 cut(s) 328, 492, 835, 3688
ScaI AGTACT 2 cut(s) 439, 2361
SchI GAGTC 4 cut(s) 1165, 1806, 1921, 3668
ScrFI CCNGG 6 cut(s) 933, 990, 2934, 3291, 3558, 3692
SduI GDGCHC 3 cut(s) 503, 2217, 2613
SfaNI GCATC 3 cut(s) 645, 1260, 1384
SfoI GGCGCC 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 3766
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 3 cut(s) 328, 835, 3688
SlaI CTCGAG 1 cut(s) 3766
SmiMI CAYNNNNRTG 3 cut(s) 912, 1863, 2004
SmlI CTYRAG 5 cut(s) 1924, 2813, 3254, 3568, 3766
SmoI CTYRAG 5 cut(s) 1924, 2813, 3254, 3568, 3766
SpeI ACTAGT 1 cut(s) 2996
SphI GCATGC 1 cut(s) 94
SseBI AGGCCT 1 cut(s) 2957
SsiI CCGC 9 cut(s) 6, 9, 88, 228, 325, 1765, 1784, 3177, 3830
SspDI GGCGCC 1 cut(s) 40
SspI AATATT 3 cut(s) 1463, 2800, 2863
SspMI CTAG 9 cut(s) 615, 732, 1191, 1689, 1965, 2042, 2664, 2997, 3928
SstI GAGCTC 2 cut(s) 503, 2613
StuI AGGCCT 1 cut(s) 2957
StyD4I CCNGG 6 cut(s) 931, 988, 2932, 3289, 3556, 3690
StyI CCWWGG 5 cut(s) 1964, 2145, 2208, 2268, 3284
TaaI ACNGT 8 cut(s) 901, 1589, 2239, 2264, 2282, 2650, 3725, 3857
TaiI ACGT 3 cut(s) 339, 2004, 3565
TaqI TCGA 7 cut(s) 122, 318, 1240, 2050, 2217, 3767, 3815
TaqII GACCGA 1 cut(s) 225
TatI WGTACW 5 cut(s) 355, 437, 2295, 2359, 3424
TauI GCSGC 3 cut(s) 8, 11, 1786
TscAI CASTG 9 cut(s) 191, 698, 914, 1176, 2321, 2384, 2410, 2878, 3730
TseFI GTSAC 6 cut(s) 857, 1162, 1171, 1820, 1959, 2873
TseI GCWGC 4 cut(s) 589, 1308, 2489, 2540
Tsp45I GTSAC 6 cut(s) 857, 1162, 1171, 1820, 1959, 2873
TspGWI ACGGA 5 cut(s) 191, 398, 814, 1307, 1808
TspRI CASTG 9 cut(s) 191, 698, 914, 1176, 2321, 2384, 2410, 2878, 3730
Van91I CCANNNNNTGG 2 cut(s) 174, 2274
Vha464I CTTAAG 1 cut(s) 3254
VpaK11BI GGWCC 3 cut(s) 328, 835, 3688
VspI ATTAAT 3 cut(s) 105, 1056, 2511
XapI RAATTY 8 cut(s) 146, 350, 742, 979, 1675, 2221, 3066, 3598
XceI RCATGY 5 cut(s) 94, 344, 999, 1514, 2787
XhoI CTCGAG 1 cut(s) 3766
XmaJI CCTAGG 1 cut(s) 1964
XmiI GTMKAC 1 cut(s) 2501
XmnI GAANNNNTTC 3 cut(s) 218, 1033, 2957
XspI CTAG 9 cut(s) 615, 732, 1191, 1689, 1965, 2042, 2664, 2997, 3928
ZrmI AGTACT 2 cut(s) 439, 2361
Zsp2I ATGCAT 1 cut(s) 3419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.