Rroxscaffold_3G00226950

Pyruvate phosphate dikinase, PEP/pyruvate binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004913
Physical Location & Seq
Reverse (-)
10766627 .. 10772194
5568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00226950.1

Sequence Viewer

Length: 1050 bp
ATGTTTCAGAAGTTACTTGAATCTCGTATTGAGCTTCGCCCTCTTCTGATCACCTCCCAAAGAAGACTAAAAGATATACTGTTCCTTGATCTTGCTTTGGCTTCTGCTGTCAAAACAACTATGGAAAGGGGACTCACAGATTTACATTTTTCTAACCCACCGGACTGGTACCGTATTAGTGAACTGTATATACCCAATCATGGTCAGTGGCCATTACAATCGAAGGCTATTCTTGATCGCTTACAATTAGTACTTGCTGATAGGTCTCAGTGCTACCAGAGGAAGATCCAGCCCAGTGCAAAATATCTTGGTAATTTGCTTGGTGTTCAGAAGTCAGTGTGGGGGGTTGTGTCCGGCAGTTGGCAGGTTATCAGCCCAGTTGAAGTGCAGGGTATTATAGCTTCGGTGGATGAGCTAATTAGTATTCAGAACAAAGTCTATAAAAAGCCAACCATTCTAATTGCAACTAGAGTAACTGGGGAAGAGGAGATTGCAGATGGAGTAGTTGCTGTCCTGACACCTGATACGCCAGTTGTTCTATCTCATGTCTCTATAAGAGCAAGAAATAGCAAGGTATGCTTTGCTACCTGCTTTGACACAAATCTTCTCAGGAATCTCAGGTTGAAAGAAGGCAAACCAATGTCGATAGAAGTAAAGTCGGCAAAATATTATTATGAGGTTGGTGCAAAATCTTGCAACATAAGATTTTTGAGAGGTAGGATGCCAGCATGGATTAAAATCCATGTCAGTTGCCATACATTTGGAGCCTTTGAAAAGGTCCTATCAGAAGATATTAATAAGACCCATCAACTTAAGAGCATAATGAAGAGTTCAAGAATACCTTGGCCTGGTGATGAAGGTGACAAGAGGTGGAATCAAGCTTGGTGTGCAATAAAGAAGATAGTTAAGGGCTTTGGGGAGACTTTGGTGGGTGCATATCCTGGACGAGCCATGAGCTTCATAACCCAAAAACACAATCTAAACTCTCCTATTGTAAGAGAAACTTTTTCAGATGACAATATTTGCGTCATTCACGAATTTACATCTTAA

Protein Analysis

349

Amino Acids

39.53

Weight (kDa)

9.45

Isoelectric Point (pI)

39.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWD1_pHisD PF22973 120 - 227 1.9e-47 Alpha-glucan water dikinase, phosphohistidine-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 355, 596
Acc65I GGTACC 1 cut(s) 168
AccB1I GGYRCC 1 cut(s) 168
AclWI GGATC 1 cut(s) 280
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 1 cut(s) 1037
AfaI GTAC 2 cut(s) 170, 252
AfiI CCNNNNNNNGG 3 cut(s) 200, 360, 848
AflII CTTAAG 1 cut(s) 812
AgsI TTSAA 5 cut(s) 20, 383, 625, 773, 834
AjnI CCWGG 2 cut(s) 847, 940
AluBI AGCT 5 cut(s) 34, 401, 415, 881, 957
AluI AGCT 5 cut(s) 34, 401, 415, 881, 957
Alw26I GTCTC 3 cut(s) 270, 553, 914
AlwI GGATC 1 cut(s) 280
AoxI GGCC 2 cut(s) 209, 845
ApoI RAATTY 1 cut(s) 1037
AseI ATTAAT 1 cut(s) 795
Asp718I GGTACC 1 cut(s) 168
AspS9I GGNCC 1 cut(s) 778
AsuHPI GGTGA 3 cut(s) 43, 863, 872
AvaII GGWCC 1 cut(s) 778
BaeI ACNNNNGTAYC 2 cut(s) 516, 549
BalI TGGCCA 1 cut(s) 211
BanI GGYRCC 1 cut(s) 168
BarI GAAGNNNNNNTAC 2 cut(s) 385, 417
BbsI GAAGAC 1 cut(s) 70
BccI CCATC 2 cut(s) 491, 813
BciT130I CCWGG 2 cut(s) 849, 942
BclI TGATCA 1 cut(s) 48
BcoDI GTCTC 3 cut(s) 270, 553, 914
BfaI CTAG 1 cut(s) 468
BfrI CTTAAG 1 cut(s) 812
BfuAI ACCTGC 2 cut(s) 355, 596
BmcAI AGTACT 1 cut(s) 252
Bme1390I CCNGG 2 cut(s) 849, 942
Bme18I GGWCC 1 cut(s) 778
BmgT120I GGNCC 1 cut(s) 778
BmiI GGNNCC 2 cut(s) 170, 766
BmrFI CCNGG 2 cut(s) 849, 942
BmrI ACTGGG 3 cut(s) 288, 371, 486
BmsI GCATC 1 cut(s) 711
BmuI ACTGGG 3 cut(s) 288, 371, 486
BpiI GAAGAC 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 737
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 842
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 360, 848
Bse1I ACTGG 5 cut(s) 170, 294, 377, 481, 530
Bse8I GATNNNNATC 1 cut(s) 737
BseBI CCWGG 2 cut(s) 849, 942
BseDI CCNNGG 1 cut(s) 842
BseGI GGATG 2 cut(s) 415, 726
BseJI GATNNNNATC 1 cut(s) 737
BseLI CCNNNNNNNGG 3 cut(s) 200, 360, 848
BseMII CTCAG 3 cut(s) 281, 622, 631
BseNI ACTGG 5 cut(s) 170, 294, 377, 481, 530
BseRI GAGGAG 1 cut(s) 500
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 2 cut(s) 211, 847
BshNI GGYRCC 1 cut(s) 168
BsiSI CCGG 2 cut(s) 161, 354
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 3 cut(s) 200, 360, 848
BsmAI GTCTC 3 cut(s) 270, 553, 914
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 2 cut(s) 211, 847
Bso31I GGTCTC 1 cut(s) 270
Bsp143I GATC 4 cut(s) 48, 88, 235, 285
BspANI GGCC 2 cut(s) 211, 847
BspCNI CTCAG 3 cut(s) 280, 621, 630
BspLI GGNNCC 2 cut(s) 170, 766
BspMI ACCTGC 2 cut(s) 355, 596
BspPI GGATC 1 cut(s) 280
BspT107I GGYRCC 1 cut(s) 168
BspTI CTTAAG 1 cut(s) 812
BspTNI GGTCTC 1 cut(s) 270
BsrI ACTGG 5 cut(s) 170, 294, 377, 481, 530
BssECI CCNNGG 1 cut(s) 842
BssMI GATC 4 cut(s) 48, 88, 235, 285
BssT1I CCWWGG 1 cut(s) 842
Bst2UI CCWGG 2 cut(s) 849, 942
Bst4CI ACNGT 3 cut(s) 81, 173, 186
Bst6I CTCTTC 3 cut(s) 48, 477, 821
BstAFI CTTAAG 1 cut(s) 812
BstAPI GCANNNNNTGC 1 cut(s) 576
BstC8I GCNNGC 1 cut(s) 726
BstDEI CTNAG 3 cut(s) 267, 608, 617
BstF5I GGATG 2 cut(s) 415, 726
BstKTI GATC 4 cut(s) 51, 91, 238, 288
BstMAI GTCTC 3 cut(s) 270, 553, 914
BstMBI GATC 4 cut(s) 48, 88, 235, 285
BstMWI GCNNNNNNNGC 2 cut(s) 576, 887
BstNI CCWGG 2 cut(s) 849, 942
BstSCI CCNGG 2 cut(s) 847, 940
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 285
BstXI CCANNNNNNTGG 2 cut(s) 165, 761
BstYI RGATCY 1 cut(s) 285
BsuRI GGCC 2 cut(s) 211, 847
BtsCI GGATG 2 cut(s) 415, 726
BtsIMutI CAGTG 4 cut(s) 212, 275, 301, 342
BveI ACCTGC 2 cut(s) 355, 596
Cac8I GCNNGC 1 cut(s) 726
Cfr13I GGNCC 1 cut(s) 778
CseI GACGC 1 cut(s) 1015
Csp6I GTAC 2 cut(s) 169, 251
CviAII CATG 5 cut(s) 200, 545, 729, 743, 952
CviQI GTAC 2 cut(s) 169, 251
DdeI CTNAG 3 cut(s) 267, 608, 617
DpnI GATC 4 cut(s) 50, 90, 237, 287
DpnII GATC 4 cut(s) 48, 88, 235, 285
EaeI YGGCCR 1 cut(s) 209
Eam1104I CTCTTC 3 cut(s) 48, 477, 821
EarI CTCTTC 3 cut(s) 48, 477, 821
Eco130I CCWWGG 1 cut(s) 842
Eco31I GGTCTC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 778
EcoO109I RGGNCCY 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 847, 940
EcoT14I CCWWGG 1 cut(s) 842
ErhI CCWWGG 1 cut(s) 842
FaeI CATG 5 cut(s) 203, 548, 732, 746, 955
FalI AAGNNNNNCTT 6 cut(s) 563, 595, 826, 858, 988, 1020
FaqI GGGAC 1 cut(s) 144
FatI CATG 5 cut(s) 199, 544, 728, 742, 951
FbaI TGATCA 1 cut(s) 48
FokI GGATG 2 cut(s) 422, 733
FspBI CTAG 1 cut(s) 468
HaeIII GGCC 2 cut(s) 211, 847
HapII CCGG 2 cut(s) 161, 354
HgaI GACGC 1 cut(s) 1015
Hin1II CATG 5 cut(s) 203, 548, 732, 746, 955
HindIII AAGCTT 1 cut(s) 879
HinfI GANTC 4 cut(s) 20, 132, 613, 874
HpaII CCGG 2 cut(s) 161, 354
HphI GGTGA 3 cut(s) 43, 863, 872
Hpy166II GTNNAC 1 cut(s) 182
Hpy188I TCNGA 6 cut(s) 9, 48, 330, 429, 787, 1012
Hpy188III TCNNGA 5 cut(s) 233, 514, 610, 834, 1034
Hpy8I GTNNAC 1 cut(s) 182
HpyAV CCTTC 3 cut(s) 217, 623, 851
HpyCH4III ACNGT 3 cut(s) 81, 173, 186
HpyCH4V TGCA 8 cut(s) 299, 388, 464, 494, 686, 696, 890, 935
HpyF10VI GCNNNNNNNGC 2 cut(s) 576, 887
HpyF3I CTNAG 3 cut(s) 267, 608, 617
Hsp92II CATG 5 cut(s) 203, 548, 732, 746, 955
KpnI GGTACC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 4 cut(s) 48, 88, 235, 285
LmnI GCTCC 1 cut(s) 764
LweI GCATC 1 cut(s) 711
MaeI CTAG 1 cut(s) 468
MaeIII GTNAC 3 cut(s) 12, 472, 860
MalI GATC 4 cut(s) 50, 90, 237, 287
MboI GATC 4 cut(s) 48, 88, 235, 285
MboII GAAGA 8 cut(s) 35, 75, 295, 494, 596, 800, 838, 910
MflI RGATCY 1 cut(s) 285
MlsI TGGCCA 1 cut(s) 211
MluCI AATT 5 cut(s) 245, 313, 417, 459, 1037
MluNI TGGCCA 1 cut(s) 211
MlyI GAGTC 1 cut(s) 126
MnlI CCTC 7 cut(s) 51, 64, 273, 478, 670, 707, 861
Mox20I TGGCCA 1 cut(s) 211
MscI TGGCCA 1 cut(s) 211
MseI TTAA 5 cut(s) 735, 795, 813, 906, 1048
Msp20I TGGCCA 1 cut(s) 211
MspCI CTTAAG 1 cut(s) 812
MspI CCGG 2 cut(s) 161, 354
MspR9I CCNGG 2 cut(s) 849, 942
MvaI CCWGG 2 cut(s) 849, 942
MwoI GCNNNNNNNGC 2 cut(s) 576, 887
NdeII GATC 4 cut(s) 48, 88, 235, 285
NlaIII CATG 5 cut(s) 203, 548, 732, 746, 955
NlaIV GGNNCC 2 cut(s) 170, 766
NmuCI GTSAC 1 cut(s) 860
PfeI GAWTC 3 cut(s) 20, 613, 874
PfoI TCCNGGA 1 cut(s) 940
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
PpuMI RGGWCCY 1 cut(s) 778
PshBI ATTAAT 1 cut(s) 795
Psp5II RGGWCCY 1 cut(s) 778
Psp6I CCWGG 2 cut(s) 847, 940
PspGI CCWGG 2 cut(s) 847, 940
PspN4I GGNNCC 2 cut(s) 170, 766
PspPI GGNCC 1 cut(s) 778
PspPPI RGGWCCY 1 cut(s) 778
PsrI GAACNNNNNNTAC 2 cut(s) 174, 206
PsuI RGATCY 1 cut(s) 285
RsaI GTAC 2 cut(s) 170, 252
RsaNI GTAC 2 cut(s) 169, 251
SaqAI TTAA 5 cut(s) 735, 795, 813, 906, 1048
Sau3AI GATC 4 cut(s) 48, 88, 235, 285
Sau96I GGNCC 1 cut(s) 778
ScaI AGTACT 1 cut(s) 252
SchI GAGTC 1 cut(s) 126
ScrFI CCNGG 2 cut(s) 849, 942
SfaNI GCATC 1 cut(s) 711
SinI GGWCC 1 cut(s) 778
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
Sse9I AATT 5 cut(s) 245, 313, 417, 459, 1037
SspI AATATT 2 cut(s) 668, 1021
SspMI CTAG 1 cut(s) 468
StyD4I CCNGG 2 cut(s) 847, 940
StyI CCWWGG 1 cut(s) 842
TaaI ACNGT 3 cut(s) 81, 173, 186
TaqI TCGA 2 cut(s) 221, 644
TasI AATT 5 cut(s) 245, 313, 417, 459, 1037
TatI WGTACW 1 cut(s) 250
TfiI GAWTC 3 cut(s) 20, 613, 874
Tru1I TTAA 5 cut(s) 735, 795, 813, 906, 1048
Tru9I TTAA 5 cut(s) 735, 795, 813, 906, 1048
TscAI CASTG 4 cut(s) 212, 275, 301, 342
TseFI GTSAC 1 cut(s) 860
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 3 cut(s) 839, 870, 949
TspRI CASTG 4 cut(s) 212, 275, 301, 342
Vha464I CTTAAG 1 cut(s) 812
VpaK11BI GGWCC 1 cut(s) 778
VspI ATTAAT 1 cut(s) 795
XapI RAATTY 1 cut(s) 1037
XspI CTAG 1 cut(s) 468
ZrmI AGTACT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.