Rh7CG460200

water dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0004913
Physical Location & Seq
Forward (+)
60969812 .. 61026515
56704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG460200.1

Sequence Viewer

Length: 1098 bp
ATGTTTCAGAAGTTACTTGAATCTCGTATTGAGCTTCGCCCTCTTCTGATCACCTCCCACAGAAGACTAAAAGATATACTGTTCCTTGATCTTGCTTTGGCTTCAGCTGTCAAAACAACTATGGAAAGGGGACTCACAGATCTACATTTTTCTAACCCACCGGTTATCAGCCCAGTTGAAGTGCAGGGTATTATAGCTTCGGTTAATGAGCTAATTAGTATTCAGAACAAAGTCTATAAAAAGCCAACCATTCTAATTGCAACTAGAGTAACTGAGGAAGAGGAGATTCCAGATGGAGTAGTTGCTGTCCTGACACCTGATAAGCCAGATGTTCTATCTCATGACTCTATAAGAGCAAGAAATAGCAAGGTATGCTTTGCCACCTGCTTTGATACGAATCTTCTCAGGGATCTCAGGTTGAAAGAAGGCAAACCAATGTCAATAGAAGTAAAGTCGGCAAATATTATTATCAGTGACAGCAGTACGTCCGTCTTTTCATTCAAGTCTTTTGCTACTCAATCCATTCATCAAGGATTTGCCTTAAAAAATAAAATTTTCTGCGGTAGATATGCTATTTCAGTCGGGGAGTTCACCAATGAGATGGTTGGTGCAAAATCTTGCAACCTAAGATTTTTGAGAGGAAGGGTGCCAACATGGATTAAAATTCCCATGTCAGTCGCCATACTATTTGGAGCCTTTGAAAAGGTCCTATCAGAAGATGTTAATAAGGATATAGCATATAAGATTTCTTCATTTTACAAATGTGTGAAGAGCGGAGACCTCTCTAAACTTCAAGCAATACAAGAAACTATTGTACAAATGAATGCTCCTTTTTCTTTGGGACGGACTGCAAGTGGAGATGGTGGCATTTCTGTCCCTAATATTTCAAAGTCGTTTGCATATGTCAGAAAGAGTGATCAAGTCTTTTTCCAAGTGCCTCAAATTATTGGTTGCAATTTAGAGGCTTTCACTAATGGAAAGGTTGTAAGCAAACTGGCCAACTTTAATGACAAGTATGAAGTGAGAGAGGGTTGCGGCATTATGGGTTCCCAACTCCAGGACACTACTAAGGCCAAGAAGAAACTGCTCAAGGAATAA

Protein Analysis

365

Amino Acids

40.47

Weight (kDa)

9.3

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWD1_pHisD PF22973 55 - 163 6.9e-47 Alpha-glucan water dikinase, phosphohistidine-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 392
Acc36I ACCTGC 1 cut(s) 392
AccB1I GGYRCC 1 cut(s) 646
AccBSI CCGCTC 1 cut(s) 774
AciI CCGC 3 cut(s) 561, 774, 1035
AclWI GGATC 1 cut(s) 417
AcoI YGGCCR 1 cut(s) 996
AcsI RAATTY 2 cut(s) 552, 663
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 2 cut(s) 484, 816
AfiI CCNNNNNNNGG 1 cut(s) 1057
AgeI ACCGGT 1 cut(s) 160
AgsI TTSAA 7 cut(s) 20, 179, 421, 502, 701, 794, 888
AjnI CCWGG 1 cut(s) 1056
AluBI AGCT 4 cut(s) 34, 107, 197, 211
AluI AGCT 4 cut(s) 34, 107, 197, 211
Alw26I GTCTC 1 cut(s) 771
AlwI GGATC 1 cut(s) 417
AoxI GGCC 2 cut(s) 996, 1071
ApoI RAATTY 2 cut(s) 552, 663
AsiGI ACCGGT 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 706
AsuHPI GGTGA 2 cut(s) 43, 583
AvaII GGWCC 1 cut(s) 706
BalI TGGCCA 1 cut(s) 998
BanI GGYRCC 1 cut(s) 646
BarI GAAGNNNNNNTAC 2 cut(s) 181, 213
BbsI GAAGAC 1 cut(s) 70
BccI CCATC 3 cut(s) 287, 595, 854
BciT130I CCWGG 1 cut(s) 1058
BclI TGATCA 2 cut(s) 48, 916
BcoDI GTCTC 1 cut(s) 771
BfaI CTAG 1 cut(s) 264
BfuAI ACCTGC 1 cut(s) 392
BglII AGATCT 1 cut(s) 139
BisI GCNGC 1 cut(s) 1036
BlsI GCNGC 1 cut(s) 1037
Bme1390I CCNGG 1 cut(s) 1058
Bme18I GGWCC 1 cut(s) 706
BmgT120I GGNCC 1 cut(s) 706
BmiI GGNNCC 3 cut(s) 648, 694, 1048
BmrFI CCNGG 1 cut(s) 1058
BmrI ACTGGG 1 cut(s) 167
BmuI ACTGGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 70
BpmI CTGGAG 1 cut(s) 1040
BpuEI CTTGAG 1 cut(s) 1073
BsaBI GATNNNNATC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 771
BsaWI WCCGGW 1 cut(s) 160
Bsc4I CCNNNNNNNGG 1 cut(s) 1057
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 173, 999
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 1 cut(s) 1058
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 1 cut(s) 1057
BseMII CTCAG 3 cut(s) 264, 418, 427
BseNI ACTGG 2 cut(s) 173, 999
BseRI GAGGAG 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 203
BshFI GGCC 2 cut(s) 998, 1073
BshNI GGYRCC 1 cut(s) 646
BshTI ACCGGT 1 cut(s) 160
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 3 cut(s) 144, 855, 860
BslI CCNNNNNNNGG 1 cut(s) 1057
BsmAI GTCTC 1 cut(s) 771
BsmFI GGGAC 3 cut(s) 144, 855, 860
BsmI GAATGC 1 cut(s) 829
BsnI GGCC 2 cut(s) 998, 1073
Bso31I GGTCTC 1 cut(s) 771
Bsp1407I TGTACA 1 cut(s) 814
Bsp143I GATC 5 cut(s) 48, 88, 139, 409, 916
BspACI CCGC 3 cut(s) 561, 774, 1035
BspANI GGCC 2 cut(s) 998, 1073
BspCNI CTCAG 3 cut(s) 265, 417, 426
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 3 cut(s) 648, 694, 1048
BspMI ACCTGC 1 cut(s) 392
BspPI GGATC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 764
BspT107I GGYRCC 1 cut(s) 646
BspTNI GGTCTC 1 cut(s) 771
BsrBI CCGCTC 1 cut(s) 774
BsrFI RCCGGY 1 cut(s) 160
BsrGI TGTACA 1 cut(s) 814
BsrI ACTGG 2 cut(s) 173, 999
BssAI RCCGGY 1 cut(s) 160
BssMI GATC 5 cut(s) 48, 88, 139, 409, 916
Bst2UI CCWGG 1 cut(s) 1058
Bst4CI ACNGT 1 cut(s) 81
Bst6I CTCTTC 3 cut(s) 48, 273, 764
BstAPI GCANNNNNTGC 1 cut(s) 372
BstAUI TGTACA 1 cut(s) 814
BstDEI CTNAG 5 cut(s) 273, 404, 413, 626, 1068
BstKTI GATC 5 cut(s) 51, 91, 142, 412, 919
BstMAI GTCTC 1 cut(s) 771
BstMBI GATC 5 cut(s) 48, 88, 139, 409, 916
BstMWI GCNNNNNNNGC 1 cut(s) 372
BstNI CCWGG 1 cut(s) 1058
BstSCI CCNGG 1 cut(s) 1056
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 2 cut(s) 139, 409
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 2 cut(s) 139, 409
BsuRI GGCC 2 cut(s) 998, 1073
BtsIMutI CAGTG 1 cut(s) 478
BveI ACCTGC 1 cut(s) 392
CciI TCATGA 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 706
Csp6I GTAC 2 cut(s) 483, 815
CspAI ACCGGT 1 cut(s) 160
CspCI CAANNNNNGTGG 2 cut(s) 370, 405
CviAII CATG 3 cut(s) 341, 654, 670
CviQI GTAC 2 cut(s) 483, 815
DdeI CTNAG 5 cut(s) 273, 404, 413, 626, 1068
DpnI GATC 5 cut(s) 50, 90, 141, 411, 918
DpnII GATC 5 cut(s) 48, 88, 139, 409, 916
EaeI YGGCCR 1 cut(s) 996
Eam1104I CTCTTC 3 cut(s) 48, 273, 764
EarI CTCTTC 3 cut(s) 48, 273, 764
Eco31I GGTCTC 1 cut(s) 771
Eco47I GGWCC 1 cut(s) 706
Eco57I CTGAAG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 706
EcoRII CCWGG 1 cut(s) 1056
FaeI CATG 3 cut(s) 344, 657, 673
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FaqI GGGAC 3 cut(s) 144, 855, 860
FatI CATG 3 cut(s) 340, 653, 669
FauNDI CATATG 1 cut(s) 901
FbaI TGATCA 2 cut(s) 48, 916
Fnu4HI GCNGC 1 cut(s) 1036
Fsp4HI GCNGC 1 cut(s) 1036
FspBI CTAG 1 cut(s) 264
GluI GCNGC 1 cut(s) 1036
GsuI CTGGAG 1 cut(s) 1040
HaeIII GGCC 2 cut(s) 998, 1073
HapII CCGG 1 cut(s) 161
Hin1II CATG 3 cut(s) 344, 657, 673
HinfI GANTC 5 cut(s) 20, 132, 286, 344, 397
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 43, 583
Hpy166II GTNNAC 1 cut(s) 591
Hpy188I TCNGA 5 cut(s) 9, 48, 225, 715, 908
Hpy188III TCNNGA 3 cut(s) 290, 310, 341
Hpy8I GTNNAC 1 cut(s) 591
HpyAV CCTTC 2 cut(s) 419, 636
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 7 cut(s) 184, 260, 611, 621, 851, 899, 954
HpyF10VI GCNNNNNNNGC 1 cut(s) 372
HpyF3I CTNAG 5 cut(s) 273, 404, 413, 626, 1068
HpySE526I ACGT 1 cut(s) 485
Hsp92II CATG 3 cut(s) 344, 657, 673
Ksp22I TGATCA 2 cut(s) 48, 916
Kzo9I GATC 5 cut(s) 48, 88, 139, 409, 916
LguI GCTCTTC 1 cut(s) 764
LmnI GCTCC 2 cut(s) 692, 832
MaeI CTAG 1 cut(s) 264
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 3 cut(s) 12, 268, 473
MalI GATC 5 cut(s) 50, 90, 141, 411, 918
MbiI CCGCTC 1 cut(s) 774
MboI GATC 5 cut(s) 48, 88, 139, 409, 916
MboII GAAGA 8 cut(s) 35, 75, 290, 392, 728, 741, 781, 1090
MflI RGATCY 2 cut(s) 139, 409
MlsI TGGCCA 1 cut(s) 998
MluCI AATT 6 cut(s) 213, 255, 552, 663, 942, 955
MluNI TGGCCA 1 cut(s) 998
MlyI GAGTC 2 cut(s) 126, 338
MnlI CCTC 9 cut(s) 51, 64, 268, 274, 632, 791, 948, 955, 1021
Mox20I TGGCCA 1 cut(s) 998
MscI TGGCCA 1 cut(s) 998
MseI TTAA 5 cut(s) 204, 542, 660, 723, 1005
Msp20I TGGCCA 1 cut(s) 998
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 1058
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 1 cut(s) 1058
MwoI GCNNNNNNNGC 1 cut(s) 372
NdeI CATATG 1 cut(s) 901
NdeII GATC 5 cut(s) 48, 88, 139, 409, 916
NlaIII CATG 3 cut(s) 344, 657, 673
NlaIV GGNNCC 3 cut(s) 648, 694, 1048
NmuCI GTSAC 1 cut(s) 473
PagI TCATGA 1 cut(s) 340
PaqCI CACCTGC 1 cut(s) 392
PciSI GCTCTTC 1 cut(s) 764
PctI GAATGC 1 cut(s) 829
PfeI GAWTC 3 cut(s) 20, 286, 397
PfoI TCCNGGA 1 cut(s) 1056
PinAI ACCGGT 1 cut(s) 160
PkrI GCNGC 1 cut(s) 1037
PleI GAGTC 2 cut(s) 126, 338
PpsI GAGTC 2 cut(s) 126, 338
PpuMI RGGWCCY 1 cut(s) 706
Psp5II RGGWCCY 1 cut(s) 706
Psp6I CCWGG 1 cut(s) 1056
PspGI CCWGG 1 cut(s) 1056
PspN4I GGNNCC 3 cut(s) 648, 694, 1048
PspPI GGNCC 1 cut(s) 706
PspPPI RGGWCCY 1 cut(s) 706
PsuI RGATCY 2 cut(s) 139, 409
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 2 cut(s) 484, 816
RsaNI GTAC 2 cut(s) 483, 815
SapI GCTCTTC 1 cut(s) 764
SaqAI TTAA 5 cut(s) 204, 542, 660, 723, 1005
SatI GCNGC 1 cut(s) 1036
Sau3AI GATC 5 cut(s) 48, 88, 139, 409, 916
Sau96I GGNCC 1 cut(s) 706
SchI GAGTC 2 cut(s) 126, 338
ScrFI CCNGG 1 cut(s) 1058
SinI GGWCC 1 cut(s) 706
SmlI CTYRAG 1 cut(s) 1088
SmoI CTYRAG 1 cut(s) 1088
Sse9I AATT 6 cut(s) 213, 255, 552, 663, 942, 955
SsiI CCGC 3 cut(s) 561, 774, 1035
SspI AATATT 2 cut(s) 463, 883
SspMI CTAG 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 1056
TaaI ACNGT 1 cut(s) 81
TaiI ACGT 1 cut(s) 488
TasI AATT 6 cut(s) 213, 255, 552, 663, 942, 955
TatI WGTACW 1 cut(s) 814
TauI GCSGC 1 cut(s) 1038
TfiI GAWTC 3 cut(s) 20, 286, 397
Tru1I TTAA 5 cut(s) 204, 542, 660, 723, 1005
Tru9I TTAA 5 cut(s) 204, 542, 660, 723, 1005
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 5 cut(s) 486, 515, 741, 836, 1032
TspGWI ACGGA 2 cut(s) 478, 859
TspRI CASTG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 706
XapI RAATTY 2 cut(s) 552, 663
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.