Rh7AG410700

water dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004913
Physical Location & Seq
Forward (+)
54957029 .. 54961196
4168 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG410700.1

Sequence Viewer

Length: 963 bp
ATGATGGAGGAGTGGCACCAAAAGTTGCACAATAATAGTAGCCCAGATGATGTGATAATTTGTGAGGCCCTCATACATTACATTAAGTCTGGTTTTAGAATCGATGTTTATTGGAAAACATTAATTGCAAATGGTCTCACAAAAGAGAAGCTTGCAAGTTATGACCGCCCCATTGTCTCAGAGCCGCATTTCAGGGCTGATACTAGGGAGGGTCTCGTTCACGACCTGACGGCATACTTGAAGACATTAAAGGCTGTTCATTCAGGTGCTGACCTTGAATCTGCTATCGAGGTTTTGGTCCCCTCAAATAGGAAGTTACTTGAATCTCGTATTGAGCTTTGCCCTCTTCTGATCACCTCCCAGTCCCACAGAAGACTAAAAGATACACTGTTCCTTGATCTTGCTTTGGCTTCAGCTGTCAAAACAACTATGGAAAGGGGACTCCCAGATTTACATTTTTCTAACCCACCGGAGATTATGTTCTTCATTTCCTTGGTGCTCGAAAATTTATGCCTATCAACAGTTAACAATGAAGATCTCATTTACTGCACCAAGGACTGGTACCGTATTAGTGAACTGTCTCAGTGCTACCAGAGGAAGATCCAGCCCAGTGCAAAATATCTTGGTAATTTGCTTGGTGTTCAGAATTGGCAGGTTATCAGCCCAGTTGAAGTGCAGGGTATTTCGGTTAATGAGCTAATTAGTATTCAGAACAAAGTCTATAAAAAGCCAACCATTCTAATTGCAACTAGAGTAACTGGGGAAGAGGAGATTCCAGATGGAGTAGTTGCTGTCCTGACACCTGATACGCCAGATGTTCTATCTCATGTCTCTATAAGAGCAAGAAATAGCAAGGTATGCTTTGCCACCTGCTTTGATACGAATCTTCTCAGGGATCTCAGGTTGAAAGAAGGCAAACCAATGTCAATAGAAGTTGGTCACTGGAGTGTTGTTTCTTTGTAA

Protein Analysis

320

Amino Acids

36.21

Weight (kDa)

6.22

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWD1_pHisD PF22973 216 - 314 2.3e-45 Alpha-glucan water dikinase, phosphohistidine-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 878
Acc36I ACCTGC 2 cut(s) 643, 878
Acc65I GGTACC 1 cut(s) 561
AccB1I GGYRCC 2 cut(s) 15, 561
AccB7I CCANNNNNTGG 1 cut(s) 558
AciI CCGC 2 cut(s) 166, 185
AclWI GGATC 2 cut(s) 595, 903
AcsI RAATTY 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 2 cut(s) 309, 558
AgsI TTSAA 5 cut(s) 241, 278, 323, 671, 907
AleI CACNNNNGTG 1 cut(s) 945
AloI GAACNNNNNNTCC 2 cut(s) 464, 496
AluBI AGCT 4 cut(s) 151, 337, 416, 697
AluI AGCT 4 cut(s) 151, 337, 416, 697
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 5 cut(s) 140, 181, 218, 585, 835
AlwI GGATC 2 cut(s) 595, 903
AlwNI CAGNNNCTG 1 cut(s) 269
AoxI GGCC 1 cut(s) 66
ApoI RAATTY 1 cut(s) 505
AseI ATTAAT 1 cut(s) 122
Asp718I GGTACC 1 cut(s) 561
AspS9I GGNCC 2 cut(s) 67, 298
AsuHPI GGTGA 1 cut(s) 346
AvaII GGWCC 1 cut(s) 298
BanI GGYRCC 2 cut(s) 15, 561
BbsI GAAGAC 2 cut(s) 248, 379
Bbv12I GWGCWC 1 cut(s) 501
BccI CCATC 1 cut(s) 773
BceAI ACGGC 1 cut(s) 246
BclI TGATCA 1 cut(s) 351
BcoDI GTCTC 5 cut(s) 140, 181, 218, 585, 835
BfaI CTAG 2 cut(s) 204, 750
BfuAI ACCTGC 2 cut(s) 643, 878
BglII AGATCT 1 cut(s) 535
BisI GCNGC 1 cut(s) 185
BlsI GCNGC 1 cut(s) 186
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 2 cut(s) 67, 298
BmiI GGNNCC 3 cut(s) 17, 300, 563
BmrI ACTGGG 4 cut(s) 355, 603, 659, 768
BmuI ACTGGG 4 cut(s) 355, 603, 659, 768
BpiI GAAGAC 2 cut(s) 248, 379
Bsa29I ATCGAT 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 882
BsaI GGTCTC 2 cut(s) 140, 218
BsaJI CCNNGG 2 cut(s) 492, 552
BsaWI WCCGGW 1 cut(s) 469
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 558
Bse1I ACTGG 6 cut(s) 361, 563, 609, 665, 763, 947
Bse8I GATNNNNATC 1 cut(s) 882
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 492, 552
BseJI GATNNNNATC 1 cut(s) 882
BseLI CCNNNNNNNGG 2 cut(s) 309, 558
BseMII CTCAG 4 cut(s) 192, 596, 904, 913
BseNI ACTGG 6 cut(s) 361, 563, 609, 665, 763, 947
BseRI GAGGAG 2 cut(s) 23, 782
BsgI GTGCAG 2 cut(s) 532, 695
BshFI GGCC 1 cut(s) 68
BshNI GGYRCC 2 cut(s) 15, 561
BshVI ATCGAT 1 cut(s) 102
BsiHKAI GWGCWC 1 cut(s) 501
BsiSI CCGG 1 cut(s) 470
BslFI GGGAC 3 cut(s) 284, 349, 453
BslI CCNNNNNNNGG 2 cut(s) 309, 558
BsmAI GTCTC 5 cut(s) 140, 181, 218, 585, 835
BsmFI GGGAC 3 cut(s) 284, 349, 453
BsnI GGCC 1 cut(s) 68
Bso31I GGTCTC 2 cut(s) 140, 218
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 5 cut(s) 351, 397, 535, 600, 895
BspACI CCGC 2 cut(s) 166, 185
BspANI GGCC 1 cut(s) 68
BspCNI CTCAG 4 cut(s) 191, 595, 903, 912
BspDI ATCGAT 1 cut(s) 102
BspLI GGNNCC 3 cut(s) 17, 300, 563
BspMI ACCTGC 2 cut(s) 643, 878
BspPI GGATC 2 cut(s) 595, 903
BspT107I GGYRCC 2 cut(s) 15, 561
BspTNI GGTCTC 2 cut(s) 140, 218
BsrI ACTGG 6 cut(s) 361, 563, 609, 665, 763, 947
BssECI CCNNGG 2 cut(s) 492, 552
BssMI GATC 5 cut(s) 351, 397, 535, 600, 895
BssT1I CCWWGG 2 cut(s) 492, 552
Bst4CI ACNGT 4 cut(s) 390, 523, 566, 579
Bst6I CTCTTC 2 cut(s) 351, 759
BstAPI GCANNNNNTGC 1 cut(s) 858
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 4 cut(s) 178, 582, 890, 899
BstENI CCTNNNNNAGG 1 cut(s) 307
BstKTI GATC 5 cut(s) 354, 400, 538, 603, 898
BstMAI GTCTC 5 cut(s) 140, 181, 218, 585, 835
BstMBI GATC 5 cut(s) 351, 397, 535, 600, 895
BstMWI GCNNNNNNNGC 1 cut(s) 858
BstV2I GAAGAC 2 cut(s) 248, 379
BstX2I RGATCY 3 cut(s) 535, 600, 895
BstYI RGATCY 3 cut(s) 535, 600, 895
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 1 cut(s) 68
BsuTUI ATCGAT 1 cut(s) 102
BtsIMutI CAGTG 4 cut(s) 386, 590, 616, 940
BveI ACCTGC 2 cut(s) 643, 878
Cac8I GCNNGC 1 cut(s) 153
CaiI CAGNNNCTG 1 cut(s) 269
Cfr13I GGNCC 2 cut(s) 67, 298
ClaI ATCGAT 1 cut(s) 102
Csp6I GTAC 1 cut(s) 562
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviAII CATG 1 cut(s) 827
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 4 cut(s) 178, 582, 890, 899
DpnI GATC 5 cut(s) 353, 399, 537, 602, 897
DpnII GATC 5 cut(s) 351, 397, 535, 600, 895
Eam1104I CTCTTC 2 cut(s) 351, 759
EarI CTCTTC 2 cut(s) 351, 759
Eco130I CCWWGG 2 cut(s) 492, 552
Eco31I GGTCTC 2 cut(s) 140, 218
Eco47I GGWCC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 396
EcoNI CCTNNNNNAGG 1 cut(s) 307
EcoO109I RGGNCCY 1 cut(s) 67
EcoT14I CCWWGG 2 cut(s) 492, 552
ErhI CCWWGG 2 cut(s) 492, 552
FaeI CATG 1 cut(s) 830
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 845, 877
FaqI GGGAC 3 cut(s) 284, 349, 453
FatI CATG 1 cut(s) 826
FbaI TGATCA 1 cut(s) 351
Fnu4HI GCNGC 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 185
FspBI CTAG 2 cut(s) 204, 750
GluI GCNGC 1 cut(s) 185
HaeIII GGCC 1 cut(s) 68
HapII CCGG 1 cut(s) 470
Hin1II CATG 1 cut(s) 830
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 6 cut(s) 99, 278, 323, 441, 772, 883
HpaI GTTAAC 1 cut(s) 526
HpaII CCGG 1 cut(s) 470
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 3 cut(s) 220, 526, 575
Hpy188I TCNGA 4 cut(s) 181, 351, 645, 711
Hpy188III TCNNGA 3 cut(s) 221, 776, 796
Hpy8I GTNNAC 3 cut(s) 220, 526, 575
HpyAV CCTTC 1 cut(s) 905
HpyCH4III ACNGT 4 cut(s) 390, 523, 566, 579
HpyCH4V TGCA 7 cut(s) 28, 128, 155, 549, 614, 676, 746
HpyF10VI GCNNNNNNNGC 1 cut(s) 858
HpyF3I CTNAG 4 cut(s) 178, 582, 890, 899
Hsp92II CATG 1 cut(s) 830
KpnI GGTACC 1 cut(s) 565
Ksp22I TGATCA 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 5 cut(s) 351, 397, 535, 600, 895
MaeI CTAG 2 cut(s) 204, 750
MaeIII GTNAC 3 cut(s) 315, 754, 938
MalI GATC 5 cut(s) 353, 399, 537, 602, 897
MboI GATC 5 cut(s) 351, 397, 535, 600, 895
MboII GAAGA 8 cut(s) 253, 338, 384, 475, 545, 610, 776, 878
MflI RGATCY 3 cut(s) 535, 600, 895
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 7 cut(s) 57, 123, 505, 628, 646, 699, 741
MlyI GAGTC 1 cut(s) 435
MnlI CCTC 9 cut(s) 58, 80, 202, 283, 313, 354, 367, 588, 760
MseI TTAA 5 cut(s) 84, 122, 248, 525, 690
MslI CAYNNNNRTG 2 cut(s) 264, 945
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 1 cut(s) 470
MwoI GCNNNNNNNGC 1 cut(s) 858
NdeII GATC 5 cut(s) 351, 397, 535, 600, 895
NlaIII CATG 1 cut(s) 830
NlaIV GGNNCC 3 cut(s) 17, 300, 563
NmuCI GTSAC 1 cut(s) 938
OliI CACNNNNGTG 1 cut(s) 945
PaqCI CACCTGC 1 cut(s) 878
PfeI GAWTC 5 cut(s) 99, 278, 323, 772, 883
PflMI CCANNNNNTGG 1 cut(s) 558
PkrI GCNGC 1 cut(s) 186
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
PshBI ATTAAT 1 cut(s) 122
PspN4I GGNNCC 3 cut(s) 17, 300, 563
PspPI GGNCC 2 cut(s) 67, 298
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 3 cut(s) 535, 600, 895
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 2 cut(s) 264, 945
SaqAI TTAA 5 cut(s) 84, 122, 248, 525, 690
SatI GCNGC 1 cut(s) 185
Sau3AI GATC 5 cut(s) 351, 397, 535, 600, 895
Sau96I GGNCC 2 cut(s) 67, 298
SchI GAGTC 1 cut(s) 435
SduI GDGCHC 1 cut(s) 501
SinI GGWCC 1 cut(s) 298
SmiMI CAYNNNNRTG 2 cut(s) 264, 945
Sse9I AATT 7 cut(s) 57, 123, 505, 628, 646, 699, 741
SsiI CCGC 2 cut(s) 166, 185
SspMI CTAG 2 cut(s) 204, 750
StyI CCWWGG 2 cut(s) 492, 552
TaaI ACNGT 4 cut(s) 390, 523, 566, 579
TaqI TCGA 3 cut(s) 102, 288, 501
TasI AATT 7 cut(s) 57, 123, 505, 628, 646, 699, 741
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 5 cut(s) 99, 278, 323, 772, 883
Tru1I TTAA 5 cut(s) 84, 122, 248, 525, 690
Tru9I TTAA 5 cut(s) 84, 122, 248, 525, 690
TscAI CASTG 4 cut(s) 393, 590, 616, 947
TseFI GTSAC 1 cut(s) 938
Tsp45I GTSAC 1 cut(s) 938
TspDTI ATGAA 3 cut(s) 248, 475, 546
TspRI CASTG 4 cut(s) 393, 590, 616, 947
Van91I CCANNNNNTGG 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 298
VspI ATTAAT 1 cut(s) 122
XagI CCTNNNNNAGG 1 cut(s) 307
XapI RAATTY 1 cut(s) 505
XspI CTAG 2 cut(s) 204, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.