Rh7DG428800

water dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004913
Physical Location & Seq
Forward (+)
60583612 .. 60634097
50486 bp
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UTR
Exon/CDS
Intron
Rh7DG428800.1

Sequence Viewer

Length: 420 bp
ATGATCCAGCCCAGTGCAAAATATCTTGGTAATTTGCTTGGTGTTCAGAAGCCAGCGATTGATACTTCTTCTGAGAAGTTAATAAGGGCTGGATCAGAGACTATTCTGTCTACTCTCATCAATCGTTTTGACTCCATTCTGAGAGAAGCGGCTAACTTAGGCCGTTGGCAGGTTGTCAGCCCAGTTGAAGTGCAGGGTTTTATAACTTCGGTTAATGAGCTAATTAGTATCCAGAACAAAGTCTATAAAAAGGCAACCATTCTAATTGCAACTAGAGTAACTGGGGAAGAGGAGATTCCAGATGGAGTAGTTGCTGTCCTGACACCTGATTCGCCAGATTTTCTATCTCATGTCTCTATAAGAGCAAGAAATAGCAAGGGGACGAATATCCAGCTACTGCAGCAGCTTCAAGGAATGTGA

Protein Analysis

139

Amino Acids

15.17

Weight (kDa)

8.03

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWD1_pHisD PF22973 56 - 126 7e-34 Alpha-glucan water dikinase, phosphohistidine-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AasI GACNNNNNNGTC 1 cut(s) 106
Acc36I ACCTGC 1 cut(s) 160
AccI GTMKAC 1 cut(s) 110
AciI CCGC 1 cut(s) 149
AclWI GGATC 1 cut(s) 100
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 2 cut(s) 188, 410
AluBI AGCT 3 cut(s) 220, 394, 406
AluI AGCT 3 cut(s) 220, 394, 406
Alw26I GTCTC 2 cut(s) 92, 358
AlwI GGATC 1 cut(s) 100
AlwNI CAGNNNCTG 1 cut(s) 397
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 2 cut(s) 400, 403
ArsI GACNNNNNNTTYG 2 cut(s) 313, 345
BbvI GCAGC 2 cut(s) 412, 415
BccI CCATC 1 cut(s) 296
BceAI ACGGC 1 cut(s) 147
BciVI GTATCC 1 cut(s) 239
BcoDI GTCTC 2 cut(s) 92, 358
BfaI CTAG 1 cut(s) 273
BfmI CTRYAG 1 cut(s) 398
BfuAI ACCTGC 1 cut(s) 160
BfuI GTATCC 1 cut(s) 239
BisI GCNGC 3 cut(s) 150, 401, 404
BlsI GCNGC 3 cut(s) 151, 402, 405
BmrI ACTGGG 3 cut(s) 6, 176, 291
BmuI ACTGGG 3 cut(s) 6, 176, 291
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 3 cut(s) 12, 182, 286
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMII CTCAG 2 cut(s) 63, 131
BseNI ACTGG 3 cut(s) 12, 182, 286
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 412, 415
BsgI GTGCAG 1 cut(s) 212
BshFI GGCC 1 cut(s) 162
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 2 cut(s) 92, 358
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 1 cut(s) 162
Bsp143I GATC 2 cut(s) 3, 92
BspACI CCGC 1 cut(s) 149
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 2 cut(s) 64, 132
BspMAI CTGCAG 1 cut(s) 402
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 1 cut(s) 100
BsrI ACTGG 3 cut(s) 12, 182, 286
BssMI GATC 2 cut(s) 3, 92
Bst6I CTCTTC 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 3 cut(s) 72, 140, 157
BstKTI GATC 2 cut(s) 6, 95
BstMAI GTCTC 2 cut(s) 92, 358
BstMBI GATC 2 cut(s) 3, 92
BstMWI GCNNNNNNNGC 1 cut(s) 400
BstSFI CTRYAG 1 cut(s) 398
BstV1I GCAGC 2 cut(s) 412, 415
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 19
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 54
CaiI CAGNNNCTG 1 cut(s) 397
CviAII CATG 1 cut(s) 350
CviJI RGCY 9 cut(s) 10, 52, 89, 152, 162, 180, 220, 394, 406
CviKI_1 RGCY 9 cut(s) 10, 52, 89, 152, 162, 180, 220, 394, 406
DdeI CTNAG 3 cut(s) 72, 140, 157
DpnI GATC 2 cut(s) 5, 94
DpnII GATC 2 cut(s) 3, 92
DrdI GACNNNNNNGTC 1 cut(s) 106
DseDI GACNNNNNNGTC 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
FaeI CATG 1 cut(s) 353
FaiI YATR 4 cut(s) 203, 246, 351, 359
FaqI GGGAC 1 cut(s) 394
FatI CATG 1 cut(s) 349
FblI GTMKAC 1 cut(s) 110
Fnu4HI GCNGC 3 cut(s) 150, 401, 404
Fsp4HI GCNGC 3 cut(s) 150, 401, 404
FspBI CTAG 1 cut(s) 273
GluI GCNGC 3 cut(s) 150, 401, 404
HaeIII GGCC 1 cut(s) 162
Hin1II CATG 1 cut(s) 353
HinfI GANTC 3 cut(s) 131, 295, 329
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 4 cut(s) 48, 73, 97, 141
Hpy188III TCNNGA 3 cut(s) 232, 299, 319
Hpy8I GTNNAC 1 cut(s) 111
HpyCH4V TGCA 4 cut(s) 17, 193, 269, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 400
HpyF3I CTNAG 3 cut(s) 72, 140, 157
Hsp92II CATG 1 cut(s) 353
Kzo9I GATC 2 cut(s) 3, 92
Lsp1109I GCAGC 2 cut(s) 412, 415
MaeI CTAG 1 cut(s) 273
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 5, 94
MboI GATC 2 cut(s) 3, 92
MboII GAAGA 2 cut(s) 60, 299
MluCI AATT 3 cut(s) 31, 222, 264
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 1 cut(s) 283
MseI TTAA 2 cut(s) 80, 213
MwoI GCNNNNNNNGC 1 cut(s) 400
NdeII GATC 2 cut(s) 3, 92
NlaIII CATG 1 cut(s) 353
PfeI GAWTC 2 cut(s) 295, 329
PkrI GCNGC 3 cut(s) 151, 402, 405
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PsiI TTATAA 1 cut(s) 203
PstI CTGCAG 1 cut(s) 402
PstNI CAGNNNCTG 1 cut(s) 397
SaqAI TTAA 2 cut(s) 80, 213
SatI GCNGC 3 cut(s) 150, 401, 404
Sau3AI GATC 2 cut(s) 3, 92
SchI GAGTC 1 cut(s) 125
SetI ASST 5 cut(s) 174, 222, 328, 396, 408
SfcI CTRYAG 1 cut(s) 398
Sse9I AATT 3 cut(s) 31, 222, 264
SsiI CCGC 1 cut(s) 149
SspMI CTAG 1 cut(s) 273
TasI AATT 3 cut(s) 31, 222, 264
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 2 cut(s) 295, 329
Tru1I TTAA 2 cut(s) 80, 213
Tru9I TTAA 2 cut(s) 80, 213
TscAI CASTG 1 cut(s) 19
TseI GCWGC 2 cut(s) 400, 403
TspRI CASTG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 110
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.