Rh7BG411700

water dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0004913
Physical Location & Seq
Forward (+)
46293198 .. 46298894
5697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG411700.1

Sequence Viewer

Length: 1557 bp
ATGACATACTCTTACAAAAGGAAACATAACGTTCGGCAGTGGCTGCAAAAGCATACCACAGGACCTGCCAAAAACACAATGTCAAGTTCAGCTCTCGTGGATCTTGTTGACAAATCTCTGGGAGGAGATGATGTAGTCTCACGGAGAAGTTATCATGTTGGCAACTATGAAGTAGTGGGGGAGTATCATATCTTGGTTGCTACAAACAATAGAGGTGCTACAGTGCTTCACTGGGGTGTCTCAAAAATATCCCCCCGAGAATGGTTGGTCTCTCCAACTGAGATTTTACCTAAAAAATCAAATGTGGTTCCCGGAGCATGCCAGACTTATTTTAACGAAATGTCAATTGGTAACGGGTCCTTTCAGGTTGTAGATATTAATTTTCAGCAAAGCAATTTCGTTGGTATCCAATTTGTGATATGGTCTAGTGGATCTTCATGGATAAAAGATAATGGCGCCAACTTCTATGTAGGTGTAACACCAGTGATTTCGAGTGGAAAGGTTGATGGGGCTGGGGAAGGAATTGACAAATGGTTGCTTGATGAAATCTCGCGTAGAGAGAAAGAGGCTGAGAGATCATTATTGCACAGATTCAACATTGCTACGAAGCTCACAGAACGCTGCAAAAGTGAAGGTGAATCAGGGCTCATTGGCATGTTGGTCTGGTTGAGATTCATGTCGTGCAGGCATCTCACATGGGACAAAAACTACAATGTGAAACCTCGTGAAATTAGTGAAGCTCAAGATGGTTTTACCAGTCTACTACAGAGAGTATATTTAAACCAGCCAAAGGATCGGGATGTAGTGAGACTACTAATTGCACATGTTGGTCGCAGGGGTCAGGGAGATGTTGGACAGAAGATTCGTGGTGAAATACTTGTAATTCAGAGAAACAATGACTGCAAGAATGGCATGATGGAGGAATGCACAATAATAGACCATGATTTCACAAGCACCGTTTGTGATTTGTCCCCTAGACTGCAGGAGTGCCTGAACTTTGTCAAAGCACATCACGGTGATGAAGATATTGTTCCACTAATGGAGAAGTTACTAGAATCTCGTATTGAGCTTCGCCCTCTTCTGCTCACCTCCCTTAGAAGATTAAAAGATATGCTGTTCCTTGATATTGCTTTGGCTTCAGCTGTCAAAACAACTATGGAAAGGGGACTCACAGATCTACATTTTTGTAACCCACAGGAAATTATGTTCTTCATTTCTTTGGTGCTCGAAAATTTATGCCTGTCAACAGTCAAAAATGAAGATCTCATTTACTGCACCAAGGACTGGTACCGTATTAGTGAACTGAATGAACCCAATAATGGTCAGTGGGCATTACAATCAAAGGCTATTCTTGATCGCTTACAATTAGTAGCTGATAGGCCTCAGTGCTACCAGAAGAAGATCCAGCCCAGTGCAAAATATCTTGGTAATTTGCTTGGTGTTCAGAAGTCAGTGATTGATACTTTTTCTGAGGAGTTAATAAGGGCTGGATCAGAGACTATTCTGTTTACTCTCATCAATCGTTTTGACCCCATTCTGAGAAAAGCGGCTAACTAA

Protein Analysis

518

Amino Acids

58.83

Weight (kDa)

8.19

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ig_N_CWD1 PF23166 63 - 158 4.5e-19 Alpha-glucan water dikinase 1 Ig-like N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 73
Acc65I GGTACC 1 cut(s) 1287
AccB1I GGYRCC 2 cut(s) 455, 1287
AccB7I CCANNNNNTGG 1 cut(s) 1284
AccI GTMKAC 1 cut(s) 760
AccII CGCG 1 cut(s) 553
AciI CCGC 1 cut(s) 1547
AclI AACGTT 1 cut(s) 30
AclWI GGATC 5 cut(s) 108, 439, 801, 1396, 1498
AcsI RAATTY 1 cut(s) 1231
AcuI CTGAAG 1 cut(s) 1122
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 1 cut(s) 1289
AfiI CCNNNNNNNGG 4 cut(s) 261, 790, 1284, 1319
AflIII ACRYGT 1 cut(s) 823
AgsI TTSAA 1 cut(s) 595
AjuI GAANNNNNNNTTGG 2 cut(s) 330, 362
AleI CACNNNNGTG 1 cut(s) 234
AloI GAACNNNNNNTCC 2 cut(s) 1190, 1222
AluBI AGCT 6 cut(s) 92, 610, 740, 1069, 1142, 1373
AluI AGCT 6 cut(s) 92, 610, 740, 1069, 1142, 1373
Alw21I GWGCWC 1 cut(s) 1227
Alw26I GTCTC 5 cut(s) 142, 244, 274, 802, 1490
AlwI GGATC 5 cut(s) 108, 439, 801, 1396, 1498
AlwNI CAGNNNCTG 2 cut(s) 43, 65
Ama87I CYCGRG 1 cut(s) 255
AoxI GGCC 1 cut(s) 1379
ApeKI GCWGC 2 cut(s) 43, 621
ApoI RAATTY 1 cut(s) 1231
ArsI GACNNNNNNTTYG 2 cut(s) 846, 878
AseI ATTAAT 1 cut(s) 378
Asp718I GGTACC 1 cut(s) 1287
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 2 cut(s) 62, 357
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 4 cut(s) 647, 881, 1028, 1078
AvaI CYCGRG 1 cut(s) 255
AvaII GGWCC 2 cut(s) 62, 357
BanI GGYRCC 2 cut(s) 455, 1287
BanII GRGCYC 1 cut(s) 648
BauI CACGAG 2 cut(s) 95, 723
Bbv12I GWGCWC 1 cut(s) 1227
BbvI GCAGC 2 cut(s) 30, 608
BccI CCATC 3 cut(s) 500, 740, 910
BciVI GTATCC 1 cut(s) 416
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 5 cut(s) 142, 244, 274, 802, 1490
BfaI CTAG 3 cut(s) 426, 975, 1052
BfmI CTRYAG 3 cut(s) 219, 764, 980
BfoI RGCGCY 1 cut(s) 459
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 416
BglII AGATCT 2 cut(s) 1174, 1261
BisI GCNGC 3 cut(s) 44, 622, 1548
BlsI GCNGC 3 cut(s) 45, 623, 1549
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 2 cut(s) 62, 357
BmeT110I CYCGRG 1 cut(s) 255
BmgT120I GGNCC 2 cut(s) 62, 357
BmiI GGNNCC 4 cut(s) 309, 358, 457, 1289
BmrFI CCNGG 1 cut(s) 312
BmrI ACTGGG 2 cut(s) 241, 1404
BmsI GCATC 1 cut(s) 697
BmuI ACTGGG 2 cut(s) 241, 1404
BpuEI CTTGAG 1 cut(s) 726
BpuMI CCSGG 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 274
BsaJI CCNNGG 1 cut(s) 1278
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 790, 1284, 1319
Bse1I ACTGG 5 cut(s) 236, 482, 756, 1289, 1410
Bse3DI GCAATG 1 cut(s) 597
BseDI CCNNGG 1 cut(s) 1278
BseGI GGATG 1 cut(s) 805
BseLI CCNNNNNNNGG 4 cut(s) 261, 790, 1284, 1319
BseMI GCAATG 1 cut(s) 597
BseMII CTCAG 5 cut(s) 270, 561, 1397, 1461, 1529
BseNI ACTGG 5 cut(s) 236, 482, 756, 1289, 1410
BseRI GAGGAG 2 cut(s) 138, 1487
BseXI GCAGC 2 cut(s) 30, 608
BseYI CCCAGC 1 cut(s) 512
BsgI GTGCAG 2 cut(s) 703, 1258
Bsh1236I CGCG 1 cut(s) 553
BshFI GGCC 1 cut(s) 1381
BshNI GGYRCC 2 cut(s) 455, 1287
BsiHKAI GWGCWC 1 cut(s) 1227
BsiHKCI CYCGRG 1 cut(s) 255
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 3 cut(s) 713, 955, 1179
BslI CCNNNNNNNGG 4 cut(s) 261, 790, 1284, 1319
BsmAI GTCTC 5 cut(s) 142, 244, 274, 802, 1490
BsmFI GGGAC 3 cut(s) 713, 955, 1179
BsmI GAATGC 1 cut(s) 929
BsnI GGCC 1 cut(s) 1381
Bso31I GGTCTC 1 cut(s) 274
BsoBI CYCGRG 1 cut(s) 255
Bsp1286I GDGCHC 2 cut(s) 648, 1227
Bsp143I GATC 9 cut(s) 100, 431, 575, 793, 1174, 1261, 1354, 1401, 1490
BspACI CCGC 1 cut(s) 1547
BspANI GGCC 1 cut(s) 1381
BspCNI CTCAG 5 cut(s) 271, 562, 1396, 1462, 1530
BspFNI CGCG 1 cut(s) 553
BspLI GGNNCC 4 cut(s) 309, 358, 457, 1289
BspMAI CTGCAG 1 cut(s) 984
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 5 cut(s) 108, 439, 801, 1396, 1498
BspT107I GGYRCC 2 cut(s) 455, 1287
BspTNI GGTCTC 1 cut(s) 274
BsrDI GCAATG 1 cut(s) 597
BsrI ACTGG 5 cut(s) 236, 482, 756, 1289, 1410
BssECI CCNNGG 1 cut(s) 1278
BssMI GATC 9 cut(s) 100, 431, 575, 793, 1174, 1261, 1354, 1401, 1490
BssNI GRCGYC 1 cut(s) 456
BssSI CACGAG 2 cut(s) 95, 723
BssT1I CCWWGG 1 cut(s) 1278
Bst2BI CACGAG 2 cut(s) 95, 723
Bst4CI ACNGT 5 cut(s) 223, 958, 1016, 1249, 1292
Bst6I CTCTTC 1 cut(s) 1083
BstACI GRCGYC 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 43
BstC8I GCNNGC 2 cut(s) 319, 686
BstDEI CTNAG 6 cut(s) 279, 570, 1094, 1383, 1470, 1538
BstF5I GGATG 1 cut(s) 805
BstFNI CGCG 1 cut(s) 553
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 9 cut(s) 103, 434, 578, 796, 1177, 1264, 1357, 1404, 1493
BstMAI GTCTC 5 cut(s) 142, 244, 274, 802, 1490
BstMBI GATC 9 cut(s) 100, 431, 575, 793, 1174, 1261, 1354, 1401, 1490
BstMWI GCNNNNNNNGC 3 cut(s) 43, 49, 909
BstNSI RCATGY 3 cut(s) 321, 658, 827
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 3 cut(s) 219, 764, 980
BstUI CGCG 1 cut(s) 553
BstV1I GCAGC 2 cut(s) 30, 608
BstX2I RGATCY 5 cut(s) 100, 431, 1174, 1261, 1401
BstYI RGATCY 5 cut(s) 100, 431, 1174, 1261, 1401
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 1 cut(s) 1381
BtsCI GGATG 1 cut(s) 805
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 8 cut(s) 44, 228, 229, 489, 1331, 1391, 1417, 1458
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 319, 686
CaiI CAGNNNCTG 2 cut(s) 43, 65
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 62, 357
Csp6I GTAC 1 cut(s) 1288
CviAII CATG 9 cut(s) 155, 318, 438, 655, 676, 696, 824, 913, 941
CviQI GTAC 1 cut(s) 1288
DdeI CTNAG 6 cut(s) 279, 570, 1094, 1383, 1470, 1538
DinI GGCGCC 1 cut(s) 457
DpnI GATC 9 cut(s) 102, 433, 577, 795, 1176, 1263, 1356, 1403, 1492
DpnII GATC 9 cut(s) 100, 431, 575, 793, 1174, 1261, 1354, 1401, 1490
DraI TTTAAA 1 cut(s) 780
Eam1104I CTCTTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1083
Eco130I CCWWGG 1 cut(s) 1278
Eco147I AGGCCT 1 cut(s) 1381
Eco24I GRGCYC 1 cut(s) 648
Eco31I GGTCTC 1 cut(s) 274
Eco47I GGWCC 2 cut(s) 62, 357
Eco57I CTGAAG 1 cut(s) 1122
Eco88I CYCGRG 1 cut(s) 255
EcoO109I RGGNCCY 2 cut(s) 62, 357
EcoT14I CCWWGG 1 cut(s) 1278
EcoT38I GRGCYC 1 cut(s) 648
EgeI GGCGCC 1 cut(s) 457
EheI GGCGCC 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 1278
FaeI CATG 9 cut(s) 158, 321, 441, 658, 679, 699, 827, 916, 944
FaqI GGGAC 3 cut(s) 713, 955, 1179
FatI CATG 9 cut(s) 154, 317, 437, 654, 675, 695, 823, 912, 940
FblI GTMKAC 1 cut(s) 760
Fnu4HI GCNGC 3 cut(s) 44, 622, 1548
FokI GGATG 1 cut(s) 812
FriOI GRGCYC 1 cut(s) 648
Fsp4HI GCNGC 3 cut(s) 44, 622, 1548
FspBI CTAG 3 cut(s) 426, 975, 1052
GlaI GCGC 1 cut(s) 457
GluI GCNGC 3 cut(s) 44, 622, 1548
GsaI CCCAGC 1 cut(s) 516
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 1 cut(s) 1381
HapII CCGG 1 cut(s) 312
HhaI GCGC 1 cut(s) 458
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 9 cut(s) 158, 321, 441, 658, 679, 699, 827, 916, 944
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 2 cut(s) 109, 1245
HindII GTYRAC 2 cut(s) 109, 1245
HinfI GANTC 6 cut(s) 591, 638, 672, 862, 1055, 1167
HpaII CCGG 1 cut(s) 312
HphI GGTGA 4 cut(s) 647, 881, 1028, 1078
Hpy166II GTNNAC 5 cut(s) 109, 761, 1245, 1301, 1509
Hpy188I TCNGA 5 cut(s) 888, 1446, 1471, 1495, 1539
Hpy188III TCNNGA 4 cut(s) 725, 743, 797, 1352
Hpy8I GTNNAC 5 cut(s) 109, 761, 1245, 1301, 1509
HpyAV CCTTC 2 cut(s) 512, 626
HpyCH4III ACNGT 5 cut(s) 223, 958, 1016, 1249, 1292
HpyCH4IV ACGT 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 49, 909
HpyF3I CTNAG 6 cut(s) 279, 570, 1094, 1383, 1470, 1538
HpySE526I ACGT 1 cut(s) 30
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 9 cut(s) 158, 321, 441, 658, 679, 699, 827, 916, 944
HspAI GCGC 1 cut(s) 456
KasI GGCGCC 1 cut(s) 455
KpnI GGTACC 1 cut(s) 1291
Kzo9I GATC 9 cut(s) 100, 431, 575, 793, 1174, 1261, 1354, 1401, 1490
LmnI GCTCC 1 cut(s) 314
Lsp1109I GCAGC 2 cut(s) 30, 608
LweI GCATC 1 cut(s) 697
MaeI CTAG 3 cut(s) 426, 975, 1052
MaeII ACGT 1 cut(s) 30
MaeIII GTNAC 4 cut(s) 350, 475, 1047, 1187
MalI GATC 9 cut(s) 102, 433, 577, 795, 1176, 1263, 1356, 1403, 1492
MboI GATC 9 cut(s) 100, 431, 575, 793, 1174, 1261, 1354, 1401, 1490
MboII GAAGA 9 cut(s) 426, 871, 1034, 1070, 1110, 1201, 1271, 1408, 1411
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 5 cut(s) 100, 431, 1174, 1261, 1401
MhlI GDGCHC 2 cut(s) 648, 1227
Mly113I GGCGCC 1 cut(s) 456
MlyI GAGTC 1 cut(s) 1161
MmeI TCCRAC 2 cut(s) 299, 832
MnlI CCTC 9 cut(s) 116, 206, 559, 732, 913, 1086, 1099, 1392, 1465
MseI TTAA 5 cut(s) 333, 378, 779, 1103, 1478
MslI CAYNNNNRTG 4 cut(s) 234, 653, 1014, 1017
MspA1I CMGCKG 1 cut(s) 1142
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 312
MunI CAATTG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 929
MvnI CGCG 1 cut(s) 553
MwoI GCNNNNNNNGC 3 cut(s) 43, 49, 909
NarI GGCGCC 1 cut(s) 456
NciI CCSGG 1 cut(s) 312
NdeII GATC 9 cut(s) 100, 431, 575, 793, 1174, 1261, 1354, 1401, 1490
NlaIII CATG 9 cut(s) 158, 321, 441, 658, 679, 699, 827, 916, 944
NlaIV GGNNCC 4 cut(s) 309, 358, 457, 1289
NspI RCATGY 3 cut(s) 321, 658, 827
OliI CACNNNNGTG 1 cut(s) 234
PaeI GCATGC 1 cut(s) 321
PceI AGGCCT 1 cut(s) 1381
PciI ACATGT 1 cut(s) 823
PctI GAATGC 1 cut(s) 929
PfeI GAWTC 5 cut(s) 591, 638, 672, 862, 1055
PflMI CCANNNNNTGG 1 cut(s) 1284
PfoI TCCNGGA 1 cut(s) 310
PkrI GCNGC 3 cut(s) 45, 623, 1549
PleI GAGTC 1 cut(s) 1161
PluTI GGCGCC 1 cut(s) 459
PpsI GAGTC 1 cut(s) 1161
PpuMI RGGWCCY 2 cut(s) 62, 357
PscI ACATGT 1 cut(s) 823
PshBI ATTAAT 1 cut(s) 378
Psp1406I AACGTT 1 cut(s) 30
Psp5II RGGWCCY 2 cut(s) 62, 357
PspFI CCCAGC 1 cut(s) 512
PspN4I GGNNCC 4 cut(s) 309, 358, 457, 1289
PspPI GGNCC 2 cut(s) 62, 357
PspPPI RGGWCCY 2 cut(s) 62, 357
PstI CTGCAG 1 cut(s) 984
PstNI CAGNNNCTG 2 cut(s) 43, 65
PsuI RGATCY 5 cut(s) 100, 431, 1174, 1261, 1401
PvuII CAGCTG 1 cut(s) 1142
RsaI GTAC 1 cut(s) 1289
RsaNI GTAC 1 cut(s) 1288
RseI CAYNNNNRTG 4 cut(s) 234, 653, 1014, 1017
SaqAI TTAA 5 cut(s) 333, 378, 779, 1103, 1478
SatI GCNGC 3 cut(s) 44, 622, 1548
Sau3AI GATC 9 cut(s) 100, 431, 575, 793, 1174, 1261, 1354, 1401, 1490
Sau96I GGNCC 2 cut(s) 62, 357
SchI GAGTC 1 cut(s) 1161
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 2 cut(s) 648, 1227
SfaNI GCATC 1 cut(s) 697
SfcI CTRYAG 3 cut(s) 219, 764, 980
SfoI GGCGCC 1 cut(s) 457
SinI GGWCC 2 cut(s) 62, 357
SmiMI CAYNNNNRTG 4 cut(s) 234, 653, 1014, 1017
SmlI CTYRAG 1 cut(s) 741
SmoI CTYRAG 1 cut(s) 741
SphI GCATGC 1 cut(s) 321
SseBI AGGCCT 1 cut(s) 1381
SsiI CCGC 1 cut(s) 1547
SspDI GGCGCC 1 cut(s) 455
SspMI CTAG 3 cut(s) 426, 975, 1052
StuI AGGCCT 1 cut(s) 1381
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 1 cut(s) 1278
TaaI ACNGT 5 cut(s) 223, 958, 1016, 1249, 1292
TaiI ACGT 1 cut(s) 33
TaqI TCGA 2 cut(s) 491, 1227
TauI GCSGC 1 cut(s) 1550
TfiI GAWTC 5 cut(s) 591, 638, 672, 862, 1055
Tru1I TTAA 5 cut(s) 333, 378, 779, 1103, 1478
Tru9I TTAA 5 cut(s) 333, 378, 779, 1103, 1478
TscAI CASTG 8 cut(s) 44, 228, 236, 489, 1331, 1391, 1417, 1458
TseI GCWGC 2 cut(s) 43, 621
TspDTI ATGAA 8 cut(s) 183, 426, 558, 664, 1035, 1201, 1272, 1323
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 8 cut(s) 44, 228, 236, 489, 1331, 1391, 1417, 1458
Van91I CCANNNNNTGG 1 cut(s) 1284
VpaK11BI GGWCC 2 cut(s) 62, 357
VspI ATTAAT 1 cut(s) 378
XapI RAATTY 1 cut(s) 1231
XceI RCATGY 3 cut(s) 321, 658, 827
XmiI GTMKAC 1 cut(s) 760
XspI CTAG 3 cut(s) 426, 975, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.