AT4G24900

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007112
Physical Location & Seq
Forward (+)
12814806 .. 12817622
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24900.1

Sequence Viewer

Length: 1266 bp
ATGAAGAAACCATCGAAAAAGAGCGAAATCGAGTTCTGCACGGTGTGCCGGTTTCATCACGACCAAGGCTCGCGCCACAAGTACTTCCCTCGCCACAAAAGTTCCCTCTCTTCCTTACTCGATCGATTCAGATCGAAGATAGCTGACGTCAGATTCTTCCTGAAGAACCCTAGCGTTCTACGGCCACAAGAGCAGTCGCAAAACCGTGTTTGGTGTGTCTTCTGCGATGAGGACATTGTTGAGCTCGGTAGCTCCTTCGCCTGTTCTAAGGCTATCAATCACTTCGCAAGTTCCGATCATCTTAAGAATATCAAGCAATTTCTGTCGAAAAATGGCCCGGCAATGGATTGCATAGACGAGTTCCGAATTTCAGAAGCTGATGTAGCTAAGTGGGAGAAAAAATGTCAGTCATTTGGAAATGAAGATGCATCTTTTGAAGGGTCCTGTGGACAGCTGTCTGGGACATCTAATGATATCCACACTAAACTTGCGTTTGAAACTATGGATAGAATTAAAAAAGTTCCTGCACACCATATAAATTCATATAAATCAAATGATGTTATGCCTTTACAGTATAATACGAATGAGTATCAGATATCACTTTCAGAGATTCCTGGAGTTATACATAATGGTTCATATCTGAACATGGATGATTCCCAATTCCCTCTATGTGATGAATCAGGGAACGGGTTTGGCGAACATTCCATTCCTTGTAGAAGCAAGGACTACTCGGGCAACGGTAATTATTGCACCCAAGAGAATTATCAGGTGAGCCAAGATAAGAAACAAATTGATGGCTCTTACAATCCTCCAGGTGTGGTGGGAATGACTAGCATTTCGTCCTCACATTCCACTGATGCTGGCGGGAACGTTCACTCTGGAGCTCCTCCACCTTGGCTTGATGCAAATGATGGGGATTTTTCTAGTGTTCAACTAAACCAATCAGATGTGGCCCGCTTTCAGGCAAAGGTACCTGGAAAGAATCGTAAGCTGAATCCTAATAGGGTTGGCGCTGCATGGGCTGAAAGGAGAAAGATAGAGATAGAAATGGAGAAGAGTGGACATGTAACGAAGAGTAACATTGATCCAGACTGGCTTCCCAACTTTGGTCGGGTTTGGCAATCAGGCACTAGAAAAGAATCCAGGAAAGAATTTGAGAAGGAAAAACGGAAGTTAGTGAAAACTGAAAGCATCTCTACAGAATCAGAACCAGTCAAAATACAACCGTACATTAGCAAACGGGCGCGAAGAGAGAGTGGTGAGTAA

Protein Analysis

421

Amino Acids

47.52

Weight (kDa)

8.36

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 12 - 391 1e-110 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
Acc65I GGTACC 1 cut(s) 970
AccB1I GGYRCC 1 cut(s) 970
AccII CGCG 2 cut(s) 73, 1246
AciI CCGC 2 cut(s) 864, 955
AclI AACGTT 1 cut(s) 870
AclWI GGATC 1 cut(s) 1079
AcoI YGGCCR 1 cut(s) 182
AcsI RAATTY 3 cut(s) 366, 538, 1151
AcuI CTGAAG 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 147
AdeI CACNNNGTG 1 cut(s) 45
AfaI GTAC 3 cut(s) 83, 972, 1229
AfiI CCNNNNNNNGG 3 cut(s) 343, 961, 1106
AflII CTTAAG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 1063
AgsI TTSAA 3 cut(s) 437, 497, 932
AjnI CCWGG 4 cut(s) 613, 811, 973, 1142
AluBI AGCT 8 cut(s) 143, 244, 252, 377, 386, 454, 884, 991
AluI AGCT 8 cut(s) 143, 244, 252, 377, 386, 454, 884, 991
Alw21I GWGCWC 2 cut(s) 246, 886
AlwI GGATC 1 cut(s) 1079
AlwNI CAGNNNCTG 1 cut(s) 377
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 3 cut(s) 182, 334, 951
ApeKI GCWGC 1 cut(s) 1013
ApoI RAATTY 3 cut(s) 366, 538, 1151
ArsI GACNNNNNNTTYG 2 cut(s) 820, 852
Asp718I GGTACC 1 cut(s) 970
AspLEI GCGC 3 cut(s) 75, 1013, 1246
AspS9I GGNCC 3 cut(s) 335, 441, 952
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 1 cut(s) 781
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 1 cut(s) 441
BanI GGYRCC 1 cut(s) 970
BanII GRGCYC 2 cut(s) 246, 886
BbsI GAAGAC 1 cut(s) 211
Bbv12I GWGCWC 2 cut(s) 246, 886
BbvI GCAGC 1 cut(s) 1000
BccI CCATC 3 cut(s) 19, 788, 905
BceAI ACGGC 1 cut(s) 197
BciT130I CCWGG 4 cut(s) 615, 813, 975, 1144
BcnI CCSGG 1 cut(s) 338
BfaI CTAG 4 cut(s) 171, 831, 924, 1131
BfmI CTRYAG 1 cut(s) 1197
BfoI RGCGCY 1 cut(s) 1014
BfrI CTTAAG 1 cut(s) 302
BisI GCNGC 1 cut(s) 1014
BlsI GCNGC 1 cut(s) 1015
BmcAI AGTACT 1 cut(s) 83
Bme1390I CCNGG 5 cut(s) 338, 615, 813, 975, 1144
Bme18I GGWCC 1 cut(s) 441
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 3 cut(s) 335, 441, 952
BmiI GGNNCC 2 cut(s) 442, 972
BmrFI CCNGG 5 cut(s) 338, 615, 813, 975, 1144
BmsI GCATC 5 cut(s) 415, 437, 847, 892, 1200
BoxI GACNNNNGTC 1 cut(s) 454
BpiI GAAGAC 1 cut(s) 211
BpmI CTGGAG 3 cut(s) 636, 795, 900
BpuMI CCSGG 1 cut(s) 338
Bsa29I ATCGAT 1 cut(s) 124
BsaBI GATNNNNATC 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 2 cut(s) 64, 893
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 961, 1106
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 2 cut(s) 1097, 1211
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 1 cut(s) 130
BseBI CCWGG 4 cut(s) 615, 813, 975, 1144
BseCI ATCGAT 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 64, 893
BseGI GGATG 1 cut(s) 655
BseJI GATNNNNATC 1 cut(s) 130
BseLI CCNNNNNNNGG 3 cut(s) 343, 961, 1106
BseMI GCAATG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 1097, 1211
BseRI GAGGAG 1 cut(s) 876
BseXI GCAGC 1 cut(s) 1000
BsgI GTGCAG 2 cut(s) 22, 510
Bsh1236I CGCG 2 cut(s) 73, 1246
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 3 cut(s) 184, 336, 953
BshNI GGYRCC 1 cut(s) 970
BshVI ATCGAT 1 cut(s) 124
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 2 cut(s) 246, 886
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 2 cut(s) 49, 338
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 3 cut(s) 343, 961, 1106
BsmFI GGGAC 1 cut(s) 475
BsnI GGCC 3 cut(s) 184, 336, 953
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 2 cut(s) 246, 886
Bsp143I GATC 4 cut(s) 121, 131, 295, 1084
BspACI CCGC 2 cut(s) 864, 955
BspANI GGCC 3 cut(s) 184, 336, 953
BspDI ATCGAT 1 cut(s) 124
BspFNI CGCG 2 cut(s) 73, 1246
BspLI GGNNCC 2 cut(s) 442, 972
BspPI GGATC 1 cut(s) 1079
BspT107I GGYRCC 1 cut(s) 970
BspTI CTTAAG 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 348
BsrFI RCCGGY 1 cut(s) 48
BsrI ACTGG 2 cut(s) 1097, 1211
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 2 cut(s) 64, 893
BssMI GATC 4 cut(s) 121, 131, 295, 1084
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 64, 893
Bst2UI CCWGG 4 cut(s) 615, 813, 975, 1144
Bst4CI ACNGT 5 cut(s) 43, 206, 573, 740, 1227
Bst6I CTCTTC 4 cut(s) 115, 1049, 1067, 1243
BstACI GRCGYC 1 cut(s) 147
BstAFI CTTAAG 1 cut(s) 302
BstAPI GCANNNNNTGC 1 cut(s) 45
BstC8I GCNNGC 3 cut(s) 71, 862, 955
BstDEI CTNAG 2 cut(s) 267, 387
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 2 cut(s) 73, 1246
BstH2I RGCGCY 1 cut(s) 1014
BstHHI GCGC 3 cut(s) 75, 1013, 1246
BstKTI GATC 4 cut(s) 124, 134, 298, 1087
BstMBI GATC 4 cut(s) 121, 131, 295, 1084
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 45, 190, 383, 1019
BstNI CCWGG 4 cut(s) 615, 813, 975, 1144
BstNSI RCATGY 1 cut(s) 1067
BstPAI GACNNNNGTC 1 cut(s) 454
BstSCI CCNGG 5 cut(s) 336, 613, 811, 973, 1142
BstSFI CTRYAG 1 cut(s) 1197
BstUI CGCG 2 cut(s) 73, 1246
BstV1I GCAGC 1 cut(s) 1000
BstV2I GAAGAC 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 124
BsuRI GGCC 3 cut(s) 184, 336, 953
BsuTUI ATCGAT 1 cut(s) 124
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 1 cut(s) 655
BtsIMutI CAGTG 1 cut(s) 852
Cac8I GCNNGC 3 cut(s) 71, 862, 955
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 3 cut(s) 75, 1013, 1246
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 3 cut(s) 335, 441, 952
ClaI ATCGAT 1 cut(s) 124
Csp6I GTAC 3 cut(s) 82, 971, 1228
CviAII CATG 3 cut(s) 646, 1017, 1064
CviQI GTAC 3 cut(s) 82, 971, 1228
DdeI CTNAG 2 cut(s) 267, 387
DpnI GATC 4 cut(s) 123, 133, 297, 1086
DpnII GATC 4 cut(s) 121, 131, 295, 1084
DraIII CACNNNGTG 1 cut(s) 45
EaeI YGGCCR 1 cut(s) 182
Eam1104I CTCTTC 4 cut(s) 115, 1049, 1067, 1243
EarI CTCTTC 4 cut(s) 115, 1049, 1067, 1243
Ecl136II GAGCTC 2 cut(s) 244, 884
Eco130I CCWWGG 2 cut(s) 64, 893
Eco24I GRGCYC 2 cut(s) 246, 886
Eco32I GATATC 2 cut(s) 475, 597
Eco47I GGWCC 1 cut(s) 441
Eco53kI GAGCTC 2 cut(s) 244, 884
Eco57I CTGAAG 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 730
EcoICRI GAGCTC 2 cut(s) 244, 884
EcoO109I RGGNCCY 1 cut(s) 441
EcoRII CCWGG 4 cut(s) 613, 811, 973, 1142
EcoRV GATATC 2 cut(s) 475, 597
EcoT14I CCWWGG 2 cut(s) 64, 893
EcoT22I ATGCAT 1 cut(s) 430
EcoT38I GRGCYC 2 cut(s) 246, 886
ErhI CCWWGG 2 cut(s) 64, 893
FaeI CATG 3 cut(s) 649, 1020, 1067
FaqI GGGAC 1 cut(s) 475
FatI CATG 3 cut(s) 645, 1016, 1063
FauI CCCGC 2 cut(s) 857, 962
Fnu4HI GCNGC 1 cut(s) 1014
FokI GGATG 1 cut(s) 662
FriOI GRGCYC 2 cut(s) 246, 886
Fsp4HI GCNGC 1 cut(s) 1014
FspBI CTAG 4 cut(s) 171, 831, 924, 1131
GlaI GCGC 3 cut(s) 74, 1012, 1245
GluI GCNGC 1 cut(s) 1014
GsuI CTGGAG 3 cut(s) 636, 795, 900
HaeII RGCGCY 1 cut(s) 1014
HaeIII GGCC 3 cut(s) 184, 336, 953
HapII CCGG 2 cut(s) 49, 338
HhaI GCGC 3 cut(s) 75, 1013, 1246
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 3 cut(s) 649, 1020, 1067
Hin6I GCGC 3 cut(s) 73, 1011, 1244
HinP1I GCGC 3 cut(s) 73, 1011, 1244
HinfI GANTC 9 cut(s) 126, 153, 610, 653, 677, 982, 994, 1139, 1202
HpaII CCGG 2 cut(s) 49, 338
HphI GGTGA 1 cut(s) 781
Hpy166II GTNNAC 3 cut(s) 449, 874, 1061
Hpy188III TCNNGA 4 cut(s) 59, 160, 879, 1088
Hpy8I GTNNAC 3 cut(s) 449, 874, 1061
HpyAV CCTTC 3 cut(s) 265, 431, 1153
HpyCH4III ACNGT 5 cut(s) 43, 206, 573, 740, 1227
HpyCH4IV ACGT 2 cut(s) 147, 870
HpyCH4V TGCA 7 cut(s) 39, 351, 428, 527, 750, 905, 1016
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 190, 383, 1019
HpyF3I CTNAG 2 cut(s) 267, 387
HpySE526I ACGT 2 cut(s) 147, 870
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 3 cut(s) 649, 1020, 1067
HspAI GCGC 3 cut(s) 73, 1011, 1244
KpnI GGTACC 1 cut(s) 974
Kzo9I GATC 4 cut(s) 121, 131, 295, 1084
LmnI GCTCC 3 cut(s) 257, 881, 889
Lsp1109I GCAGC 1 cut(s) 1000
LweI GCATC 5 cut(s) 415, 437, 847, 892, 1200
MaeI CTAG 4 cut(s) 171, 831, 924, 1131
MaeII ACGT 2 cut(s) 147, 870
MaeIII GTNAC 2 cut(s) 1066, 1076
MalI GATC 4 cut(s) 123, 133, 297, 1086
MboI GATC 4 cut(s) 121, 131, 295, 1084
MhlI GDGCHC 2 cut(s) 246, 886
MluCI AATT 9 cut(s) 317, 366, 510, 538, 659, 742, 760, 789, 1151
MnlI CCTC 7 cut(s) 99, 116, 223, 675, 819, 853, 897
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 2 cut(s) 303, 513
MspA1I CMGCKG 1 cut(s) 454
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 2 cut(s) 49, 338
MspR9I CCNGG 5 cut(s) 338, 615, 813, 975, 1144
MvaI CCWGG 4 cut(s) 615, 813, 975, 1144
MvnI CGCG 2 cut(s) 73, 1246
MwoI GCNNNNNNNGC 4 cut(s) 45, 190, 383, 1019
NciI CCSGG 1 cut(s) 338
NdeII GATC 4 cut(s) 121, 131, 295, 1084
NlaIII CATG 3 cut(s) 649, 1020, 1067
NlaIV GGNNCC 2 cut(s) 442, 972
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 1067
PciI ACATGT 1 cut(s) 1063
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 693
PfeI GAWTC 9 cut(s) 126, 153, 610, 653, 677, 982, 994, 1139, 1202
PfoI TCCNGGA 2 cut(s) 613, 1142
PkrI GCNGC 1 cut(s) 1015
Ple19I CGATCG 1 cut(s) 124
PpuMI RGGWCCY 1 cut(s) 441
PscI ACATGT 1 cut(s) 1063
PshAI GACNNNNGTC 1 cut(s) 454
Psp124BI GAGCTC 2 cut(s) 246, 886
Psp1406I AACGTT 1 cut(s) 870
Psp5II RGGWCCY 1 cut(s) 441
Psp6I CCWGG 4 cut(s) 613, 811, 973, 1142
PspGI CCWGG 4 cut(s) 613, 811, 973, 1142
PspN4I GGNNCC 2 cut(s) 442, 972
PspPI GGNCC 3 cut(s) 335, 441, 952
PspPPI RGGWCCY 1 cut(s) 441
PstNI CAGNNNCTG 1 cut(s) 377
PvuI CGATCG 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 454
RsaI GTAC 3 cut(s) 83, 972, 1229
RsaNI GTAC 3 cut(s) 82, 971, 1228
SacI GAGCTC 2 cut(s) 246, 886
SaqAI TTAA 2 cut(s) 303, 513
SatI GCNGC 1 cut(s) 1014
Sau3AI GATC 4 cut(s) 121, 131, 295, 1084
Sau96I GGNCC 3 cut(s) 335, 441, 952
ScaI AGTACT 1 cut(s) 83
ScrFI CCNGG 5 cut(s) 338, 615, 813, 975, 1144
SduI GDGCHC 2 cut(s) 246, 886
SfaNI GCATC 5 cut(s) 415, 437, 847, 892, 1200
SfcI CTRYAG 1 cut(s) 1197
SinI GGWCC 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 9 cut(s) 317, 366, 510, 538, 659, 742, 760, 789, 1151
SsiI CCGC 2 cut(s) 864, 955
SspMI CTAG 4 cut(s) 171, 831, 924, 1131
SstI GAGCTC 2 cut(s) 246, 886
StyD4I CCNGG 5 cut(s) 336, 613, 811, 973, 1142
StyI CCWWGG 2 cut(s) 64, 893
TaaI ACNGT 5 cut(s) 43, 206, 573, 740, 1227
TaiI ACGT 2 cut(s) 150, 873
TaqI TCGA 6 cut(s) 14, 30, 120, 124, 134, 326
TasI AATT 9 cut(s) 317, 366, 510, 538, 659, 742, 760, 789, 1151
TatI WGTACW 1 cut(s) 81
TfiI GAWTC 9 cut(s) 126, 153, 610, 653, 677, 982, 994, 1139, 1202
Tru1I TTAA 2 cut(s) 303, 513
Tru9I TTAA 2 cut(s) 303, 513
TscAI CASTG 1 cut(s) 859
TseI GCWGC 1 cut(s) 1013
TspDTI ATGAA 6 cut(s) 17, 44, 435, 531, 624, 690
TspGWI ACGGA 1 cut(s) 1183
TspRI CASTG 1 cut(s) 859
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 441
XapI RAATTY 3 cut(s) 366, 538, 1151
XceI RCATGY 1 cut(s) 1067
XspI CTAG 4 cut(s) 171, 831, 924, 1131
ZraI GACGTC 1 cut(s) 148
ZrmI AGTACT 1 cut(s) 83
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.