Rroxscaffold_3G00264900

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007112
Physical Location & Seq
Forward (+)
58403350 .. 58406385
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00264900.1

Sequence Viewer

Length: 1311 bp
ATGGAGGAGAAGGAGAAACGATCAAAGGCTCACTCATGGAACCCTAACCCTAAATCCAACGCCACAACAATGAAGAAGAAGACGAAGAAGAAGCACGACTTCGAGTATTGCACGGTTTGCAAGCTGAATCACGACCAAGGTCGGAGCCACAAGTACATCCCCAGCCACACCAAGTCCCTCTCTACTTTCCTCTCTCGCTTCCAGGCCAAGCTCTCCGACGTCGTTTTCTTCCTCAAAAACCCTACCTTCCTTCCCCTCGAACACGCCGCTCGCAACCGCCTCTGGTGTGTCTTCTGCGATTTCGACATTGACGAGCGAGATAGCTCCTTCGCTTGTGGCAATGCAATTAATCACCTTGCGAGTGCCGAACATCTGAAGAATTTGAAGCACTTCTTGTGGAAATACGGTGGAGGAATGGATTCTGTCGATAAATTCAGGATATTGGAAGCTGACCTTGCCAAGTGGGAGAAGAAGTGCAAGTCATTGAAGAGTGAAGCTATTTCTGGAGGACCAATGCCAGGACCTTCAAATGATATCCATAATAAACTTGAATATGGAATTTTAGATACTTTTGAAAATAATAGTAGTTCAAGTTTTTCAAATGGTGTTATGCCTTTACTATATCATACAAATGAGAATCAGGTATCCCATTCTGGACTTTCTCAAGTTGCAAACGTTGGCTGCTTTGTGCAAGATGTTACTACGTCCTCTTCTATTAATTCCAAAGGTTTAACAGTTAGTAGCCAGGGTTCTCTTATTTCTAATGGTAATAAAGGCTCAGCTGACGGCAATTTCAATAATGAAATGATGAACAAGGTGTATCAGCACGGAAGAATAACAATAGGACAGAGCAGTTCTCAAGGTGTTCAGAATCTCACTCAAATTCCTTCCAAGGGTCCGAAAGAGGCCAGTGGAAATGTGTATACTGGAGCACTTCCTCCTTGGTTTGCAGCAGATGAAGAAAATCAGCTTAGTATTCCAATACAACCAGTTTCTGGTACCCTTCTTCCTCTGTCAAATAAGTCAGGGAAGTCCAAGAAATTAAACCCAAAACGAGTGGGAGCAGCTTGGGCAGAAAGGAGAAAAAGGGAGCTGGAGATGGAACAAAGGGGTGAGATTGTCAAGAGCGATTATGATGCTAATTGGCTCCCCAATTTTGGTGGAGTTTGGCAATCTGGTACCCGGAAAGAGACTAAAAAAGAATTTGAGTTGGAGAAACTTAATTTAGTTAAGGTTGAAAGTCAATCTGAGATGCCAATCAAGATACAGCCTTACGTCAGCAAAAGGATGCGAACTGGTGCGAGTGAATAA

Protein Analysis

436

Amino Acids

48.68

Weight (kDa)

9.28

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 36 - 406 7.1e-92 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 222
Acc65I GGTACC 2 cut(s) 998, 1178
AccB1I GGYRCC 2 cut(s) 998, 1178
AccB7I CCANNNNNTGG 1 cut(s) 995
AccBSI CCGCTC 1 cut(s) 269
AccI GTMKAC 1 cut(s) 923
AciI CCGC 2 cut(s) 267, 277
AclI AACGTT 1 cut(s) 675
AcsI RAATTY 5 cut(s) 379, 431, 558, 882, 1202
AcuI CTGAAG 1 cut(s) 395
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 3 cut(s) 155, 1000, 1180
AfiI CCNNNNNNNGG 4 cut(s) 518, 893, 995, 1157
AgsI TTSAA 9 cut(s) 385, 487, 528, 551, 575, 591, 600, 796, 1238
AjnI CCWGG 3 cut(s) 201, 517, 744
AluBI AGCT 9 cut(s) 124, 211, 324, 449, 497, 782, 970, 1067, 1093
AluI AGCT 9 cut(s) 124, 211, 324, 449, 497, 782, 970, 1067, 1093
Alw21I GWGCWC 1 cut(s) 934
Alw26I GTCTC 1 cut(s) 1184
AlwNI CAGNNNCTG 1 cut(s) 995
AoxI GGCC 2 cut(s) 204, 906
ApeKI GCWGC 3 cut(s) 681, 950, 1064
ApoI RAATTY 5 cut(s) 379, 431, 558, 882, 1202
AseI ATTAAT 2 cut(s) 348, 717
Asp700I GAANNNNTTC 2 cut(s) 389, 418
Asp718I GGTACC 2 cut(s) 998, 1178
AspS9I GGNCC 3 cut(s) 509, 521, 896
AsuC2I CCSGG 1 cut(s) 1183
AsuHPI GGTGA 2 cut(s) 344, 1124
AvaII GGWCC 3 cut(s) 509, 521, 896
BanI GGYRCC 2 cut(s) 998, 1178
BbsI GAAGAC 2 cut(s) 86, 283
Bbv12I GWGCWC 1 cut(s) 934
BbvI GCAGC 3 cut(s) 668, 962, 1076
BccI CCATC 1 cut(s) 1093
BceAI ACGGC 1 cut(s) 802
BcgI CGANNNNNNTGC 2 cut(s) 1118, 1152
BciT130I CCWGG 3 cut(s) 203, 519, 746
BciVI GTATCC 1 cut(s) 655
BcnI CCSGG 1 cut(s) 1183
BcoDI GTCTC 1 cut(s) 1184
BfuI GTATCC 1 cut(s) 655
BisI GCNGC 4 cut(s) 267, 682, 951, 1065
BlpI GCTNAGC 1 cut(s) 778
BlsI GCNGC 4 cut(s) 268, 683, 952, 1066
Bme1390I CCNGG 4 cut(s) 203, 519, 746, 1183
Bme18I GGWCC 3 cut(s) 509, 521, 896
BmgT120I GGNCC 3 cut(s) 509, 521, 896
BmiI GGNNCC 6 cut(s) 41, 146, 897, 1000, 1148, 1180
BmrFI CCNGG 4 cut(s) 203, 519, 746, 1183
BmsI GCATC 3 cut(s) 1126, 1242, 1278
BoxI GACNNNNGTC 1 cut(s) 138
BpiI GAAGAC 2 cut(s) 86, 283
BplI GAGNNNNNCTC 2 cut(s) 841, 873
BpmI CTGGAG 3 cut(s) 525, 948, 1115
Bpu1102I GCTNAGC 1 cut(s) 778
BpuEI CTTGAG 2 cut(s) 648, 843
BpuMI CCSGG 1 cut(s) 1183
BsaBI GATNNNNATC 1 cut(s) 1256
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 4 cut(s) 136, 745, 891, 941
BsaXI ACNNNNNCTCC 2 cut(s) 458, 488
Bsc4I CCNNNNNNNGG 4 cut(s) 518, 893, 995, 1157
Bse1I ACTGG 4 cut(s) 909, 931, 989, 1300
Bse3DI GCAATG 1 cut(s) 346
Bse8I GATNNNNATC 1 cut(s) 1256
BseBI CCWGG 3 cut(s) 203, 519, 746
BseDI CCNNGG 4 cut(s) 136, 745, 891, 941
BseGI GGATG 2 cut(s) 156, 1293
BseJI GATNNNNATC 1 cut(s) 1256
BseLI CCNNNNNNNGG 4 cut(s) 518, 893, 995, 1157
BseMI GCAATG 1 cut(s) 346
BseMII CTCAG 2 cut(s) 792, 1239
BseNI ACTGG 4 cut(s) 909, 931, 989, 1300
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 3 cut(s) 668, 962, 1076
BseYI CCCAGC 1 cut(s) 161
BshFI GGCC 2 cut(s) 206, 908
BshNI GGYRCC 2 cut(s) 998, 1178
BsiHKAI GWGCWC 1 cut(s) 934
BsiSI CCGG 1 cut(s) 1183
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 4 cut(s) 518, 893, 995, 1157
BsmAI GTCTC 1 cut(s) 1184
BsmFI GGGAC 1 cut(s) 160
BsnI GGCC 2 cut(s) 206, 908
Bsp1286I GDGCHC 1 cut(s) 934
Bsp143I GATC 1 cut(s) 20
Bsp1720I GCTNAGC 1 cut(s) 778
BspACI CCGC 2 cut(s) 267, 277
BspANI GGCC 2 cut(s) 206, 908
BspCNI CTCAG 2 cut(s) 791, 1240
BspLI GGNNCC 6 cut(s) 41, 146, 897, 1000, 1148, 1180
BspT107I GGYRCC 2 cut(s) 998, 1178
BsrBI CCGCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 346
BsrI ACTGG 4 cut(s) 909, 931, 989, 1300
BssECI CCNNGG 4 cut(s) 136, 745, 891, 941
BssMI GATC 1 cut(s) 20
BssNAI GTATAC 1 cut(s) 924
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 3 cut(s) 136, 891, 941
Bst1107I GTATAC 1 cut(s) 924
Bst2UI CCWGG 3 cut(s) 203, 519, 746
Bst4CI ACNGT 3 cut(s) 115, 407, 736
Bst6I CTCTTC 2 cut(s) 482, 715
BstACI GRCGYC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 122, 271
BstDEI CTNAG 3 cut(s) 778, 971, 1248
BstF5I GGATG 2 cut(s) 156, 1293
BstKTI GATC 1 cut(s) 23
BstMAI GTCTC 1 cut(s) 1184
BstMBI GATC 1 cut(s) 20
BstMWI GCNNNNNNNGC 3 cut(s) 117, 455, 1070
BstNI CCWGG 3 cut(s) 203, 519, 746
BstPAI GACNNNNGTC 1 cut(s) 138
BstSCI CCNGG 4 cut(s) 201, 517, 744, 1181
BstV1I GCAGC 3 cut(s) 668, 962, 1076
BstV2I GAAGAC 2 cut(s) 86, 283
BstZ17I GTATAC 1 cut(s) 924
BsuI GTATCC 1 cut(s) 655
BsuRI GGCC 2 cut(s) 206, 908
BtsCI GGATG 2 cut(s) 156, 1293
BtsIMutI CAGTG 1 cut(s) 916
Cac8I GCNNGC 2 cut(s) 122, 271
CaiI CAGNNNCTG 1 cut(s) 995
Cfr13I GGNCC 3 cut(s) 509, 521, 896
Csp6I GTAC 3 cut(s) 154, 999, 1179
CspCI CAANNNNNGTGG 4 cut(s) 1038, 1073, 1141, 1176
CviAII CATG 1 cut(s) 36
CviQI GTAC 3 cut(s) 154, 999, 1179
DdeI CTNAG 3 cut(s) 778, 971, 1248
DpnI GATC 1 cut(s) 22
DpnII GATC 1 cut(s) 20
Eam1104I CTCTTC 2 cut(s) 482, 715
EarI CTCTTC 2 cut(s) 482, 715
Eco130I CCWWGG 3 cut(s) 136, 891, 941
Eco32I GATATC 1 cut(s) 535
Eco47I GGWCC 3 cut(s) 509, 521, 896
Eco57I CTGAAG 1 cut(s) 395
EcoO109I RGGNCCY 1 cut(s) 521
EcoRII CCWGG 3 cut(s) 201, 517, 744
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 3 cut(s) 136, 891, 941
ErhI CCWWGG 3 cut(s) 136, 891, 941
FaeI CATG 1 cut(s) 39
FaiI YATR 8 cut(s) 37, 540, 555, 611, 622, 627, 924, 1134
FalI AAGNNNNNCTT 6 cut(s) 83, 115, 377, 409, 438, 470
FaqI GGGAC 1 cut(s) 160
FatI CATG 1 cut(s) 35
FblI GTMKAC 1 cut(s) 923
Fnu4HI GCNGC 4 cut(s) 267, 682, 951, 1065
FokI GGATG 2 cut(s) 143, 1300
Fsp4HI GCNGC 4 cut(s) 267, 682, 951, 1065
GluI GCNGC 4 cut(s) 267, 682, 951, 1065
GsaI CCCAGC 1 cut(s) 165
GsuI CTGGAG 3 cut(s) 525, 948, 1115
HaeIII GGCC 2 cut(s) 206, 908
HapII CCGG 1 cut(s) 1183
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 1 cut(s) 39
HinfI GANTC 4 cut(s) 127, 419, 637, 871
HpaII CCGG 1 cut(s) 1183
HphI GGTGA 2 cut(s) 344, 1124
Hpy166II GTNNAC 1 cut(s) 924
Hpy188I TCNGA 6 cut(s) 144, 217, 375, 870, 900, 1249
Hpy188III TCNNGA 6 cut(s) 131, 436, 504, 654, 1123, 1261
Hpy8I GTNNAC 1 cut(s) 924
Hpy99I CGWCG 2 cut(s) 221, 224
HpyAV CCTTC 7 cut(s) 4, 256, 260, 337, 534, 897, 1013
HpyCH4III ACNGT 3 cut(s) 115, 407, 736
HpyCH4IV ACGT 4 cut(s) 219, 675, 704, 1275
HpyCH4V TGCA 7 cut(s) 111, 120, 344, 477, 671, 691, 950
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 455, 1070
HpyF3I CTNAG 3 cut(s) 778, 971, 1248
HpySE526I ACGT 4 cut(s) 219, 675, 704, 1275
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 1 cut(s) 39
KpnI GGTACC 2 cut(s) 1002, 1182
Kzo9I GATC 1 cut(s) 20
LmnI GCTCC 6 cut(s) 144, 329, 929, 1061, 1090, 1152
Lsp1109I GCAGC 3 cut(s) 668, 962, 1076
LweI GCATC 3 cut(s) 1126, 1242, 1278
MaeII ACGT 4 cut(s) 219, 675, 704, 1275
MaeIII GTNAC 1 cut(s) 697
MalI GATC 1 cut(s) 22
MbiI CCGCTC 1 cut(s) 269
MboI GATC 1 cut(s) 20
MhlI GDGCHC 1 cut(s) 934
MmeI TCCRAC 4 cut(s) 81, 122, 240, 1191
MroXI GAANNNNTTC 2 cut(s) 389, 418
MseI TTAA 6 cut(s) 348, 717, 731, 1043, 1221, 1230
MslI CAYNNNNRTG 2 cut(s) 68, 630
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 1 cut(s) 1183
MspR9I CCNGG 4 cut(s) 203, 519, 746, 1183
MvaI CCWGG 3 cut(s) 203, 519, 746
MwoI GCNNNNNNNGC 3 cut(s) 117, 455, 1070
NciI CCSGG 1 cut(s) 1183
NdeII GATC 1 cut(s) 20
NlaIII CATG 1 cut(s) 39
NlaIV GGNNCC 6 cut(s) 41, 146, 897, 1000, 1148, 1180
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PdmI GAANNNNTTC 2 cut(s) 389, 418
PfeI GAWTC 4 cut(s) 127, 419, 637, 871
PflMI CCANNNNNTGG 1 cut(s) 995
PkrI GCNGC 4 cut(s) 268, 683, 952, 1066
PpuMI RGGWCCY 1 cut(s) 521
PshAI GACNNNNGTC 1 cut(s) 138
PshBI ATTAAT 2 cut(s) 348, 717
Psp1406I AACGTT 1 cut(s) 675
Psp5II RGGWCCY 1 cut(s) 521
Psp6I CCWGG 3 cut(s) 201, 517, 744
PspFI CCCAGC 1 cut(s) 161
PspGI CCWGG 3 cut(s) 201, 517, 744
PspN4I GGNNCC 6 cut(s) 41, 146, 897, 1000, 1148, 1180
PspPI GGNCC 3 cut(s) 509, 521, 896
PspPPI RGGWCCY 1 cut(s) 521
PsrI GAACNNNNNNTAC 2 cut(s) 733, 765
PstNI CAGNNNCTG 1 cut(s) 995
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 3 cut(s) 155, 1000, 1180
RsaNI GTAC 3 cut(s) 154, 999, 1179
RseI CAYNNNNRTG 2 cut(s) 68, 630
SaqAI TTAA 6 cut(s) 348, 717, 731, 1043, 1221, 1230
SatI GCNGC 4 cut(s) 267, 682, 951, 1065
Sau3AI GATC 1 cut(s) 20
Sau96I GGNCC 3 cut(s) 509, 521, 896
ScrFI CCNGG 4 cut(s) 203, 519, 746, 1183
SduI GDGCHC 1 cut(s) 934
SfaNI GCATC 3 cut(s) 1126, 1242, 1278
SinI GGWCC 3 cut(s) 509, 521, 896
SmiMI CAYNNNNRTG 2 cut(s) 68, 630
SmlI CTYRAG 2 cut(s) 663, 858
SmoI CTYRAG 2 cut(s) 663, 858
SsiI CCGC 2 cut(s) 267, 277
StyD4I CCNGG 4 cut(s) 201, 517, 744, 1181
StyI CCWWGG 3 cut(s) 136, 891, 941
TaaI ACNGT 3 cut(s) 115, 407, 736
TaiI ACGT 4 cut(s) 222, 678, 707, 1278
TaqI TCGA 4 cut(s) 102, 258, 303, 426
TatI WGTACW 1 cut(s) 153
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 4 cut(s) 127, 419, 637, 871
Tru1I TTAA 6 cut(s) 348, 717, 731, 1043, 1221, 1230
Tru9I TTAA 6 cut(s) 348, 717, 731, 1043, 1221, 1230
TscAI CASTG 1 cut(s) 916
TseI GCWGC 3 cut(s) 681, 950, 1064
TspDTI ATGAA 4 cut(s) 86, 816, 824, 972
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 1 cut(s) 916
Van91I CCANNNNNTGG 1 cut(s) 995
VpaK11BI GGWCC 3 cut(s) 509, 521, 896
VspI ATTAAT 2 cut(s) 348, 717
XapI RAATTY 5 cut(s) 379, 431, 558, 882, 1202
XmiI GTMKAC 1 cut(s) 923
XmnI GAANNNNTTC 2 cut(s) 389, 418
ZraI GACGTC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.