RchiOBHm_Chr7g0190091

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007112
Physical Location & Seq
Reverse (-)
9285094 .. 9286223
1130 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16978

Sequence Viewer

Length: 750 bp
ATGGAGGAGAAGGAGAAACGATCAAAGGCTCATTCGTGGAATCCTAACCCTAACCCTAAATCCAACGCCACAACAATGAAGAAGAAGCACGACTTCGAGTATTGCACGGTTTGCAAGCTGAATCACGACCAAGGTCCGAGCCACAAGTACATCCCCAGCCACACCAAGTCCCTCTCTACTTTCCTCTCTCGCTTCCAGGCCAAGCTCTCCGACGTCGTTTTCTTCCTCAAAAACCCTACCTTCCTCCGCCTCGAACACGCCGCTCGCAACCGCCTCTGGTGCGTCTTCTGCGATTTCGACATTGACGAGCGGGATAGCTCCTTCGCTTGTGGCAATGCAATTAATCACCTTGCGAGTGCCGAACATCTGAAGAATTTGAAGCACTTCTTGTGGAAATACGGTGGAGGAATGGATTCTGTCGATAAATTCAGGATATTGGAAGCTGACCTTGCTAAGTGGGAGAAGAAGTGCAAGTCATTGAAGAGTGAAGCTATTTCTGGAGGACCAATGCCAGGACCTTCAAATGATATCCATAATAAACTTGAATATGGAATTTTAGATACTTTTGAAAATAATAGTAGTTCAAGTTTTTCAAATGGTGTTATGCCTTTACTATATCATACAAATGAGAATCAGGTATCCCATTCTGGACTTTCTCAAGTTGCAAATGTTGGCTGCTTTGTGCAAGATGTTACTGCGTCCTCTTCTATTAATTCCAAAGGTTTAACAGGTATGCATGTTTGTTTATAG

Protein Analysis

249

Amino Acids

27.94

Weight (kDa)

8.55

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 34 - 220 6.3e-52 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 216
AccBSI CCGCTC 2 cut(s) 263, 310
AciI CCGC 4 cut(s) 247, 261, 271, 310
AcsI RAATTY 3 cut(s) 373, 425, 552
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 512
AgsI TTSAA 7 cut(s) 379, 481, 522, 545, 569, 585, 594
AjnI CCWGG 2 cut(s) 195, 511
AluBI AGCT 5 cut(s) 118, 205, 318, 443, 491
AluI AGCT 5 cut(s) 118, 205, 318, 443, 491
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 1 cut(s) 675
ApoI RAATTY 3 cut(s) 373, 425, 552
AseI ATTAAT 2 cut(s) 342, 711
Asp700I GAANNNNTTC 2 cut(s) 383, 412
AspS9I GGNCC 3 cut(s) 134, 503, 515
AsuHPI GGTGA 1 cut(s) 338
AvaII GGWCC 3 cut(s) 134, 503, 515
BbsI GAAGAC 1 cut(s) 277
BbvI GCAGC 1 cut(s) 662
BciT130I CCWGG 2 cut(s) 197, 513
BciVI GTATCC 1 cut(s) 649
BfuI GTATCC 1 cut(s) 649
BisI GCNGC 2 cut(s) 261, 676
BlsI GCNGC 2 cut(s) 262, 677
Bme1390I CCNGG 2 cut(s) 197, 513
Bme18I GGWCC 3 cut(s) 134, 503, 515
BmgT120I GGNCC 3 cut(s) 134, 503, 515
BmrFI CCNGG 2 cut(s) 197, 513
BoxI GACNNNNGTC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 277
BpmI CTGGAG 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 642
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 130
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse3DI GCAATG 1 cut(s) 340
BseBI CCWGG 2 cut(s) 197, 513
BseDI CCNNGG 1 cut(s) 130
BseGI GGATG 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMI GCAATG 1 cut(s) 340
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 662
BseYI CCCAGC 1 cut(s) 155
BshFI GGCC 1 cut(s) 200
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 1 cut(s) 512
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 1 cut(s) 200
Bsp143I GATC 1 cut(s) 20
BspACI CCGC 4 cut(s) 247, 261, 271, 310
BspANI GGCC 1 cut(s) 200
BsrBI CCGCTC 2 cut(s) 263, 310
BsrDI GCAATG 1 cut(s) 340
BssECI CCNNGG 1 cut(s) 130
BssMI GATC 1 cut(s) 20
BssNI GRCGYC 1 cut(s) 213
BssT1I CCWWGG 1 cut(s) 130
Bst2UI CCWGG 2 cut(s) 197, 513
Bst4CI ACNGT 2 cut(s) 109, 401
Bst6I CTCTTC 2 cut(s) 476, 709
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 116, 265
BstDEI CTNAG 1 cut(s) 453
BstF5I GGATG 1 cut(s) 150
BstKTI GATC 1 cut(s) 23
BstMBI GATC 1 cut(s) 20
BstMWI GCNNNNNNNGC 4 cut(s) 111, 279, 288, 449
BstNI CCWGG 2 cut(s) 197, 513
BstNSI RCATGY 1 cut(s) 740
BstPAI GACNNNNGTC 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 195, 511
BstV1I GCAGC 1 cut(s) 662
BstV2I GAAGAC 1 cut(s) 277
BsuI GTATCC 1 cut(s) 649
BsuRI GGCC 1 cut(s) 200
BtsCI GGATG 1 cut(s) 150
Cac8I GCNNGC 2 cut(s) 116, 265
Cfr13I GGNCC 3 cut(s) 134, 503, 515
CseI GACGC 2 cut(s) 271, 687
Csp6I GTAC 1 cut(s) 148
CviAII CATG 1 cut(s) 737
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 453
DpnI GATC 1 cut(s) 22
DpnII GATC 1 cut(s) 20
Eam1104I CTCTTC 2 cut(s) 476, 709
EarI CTCTTC 2 cut(s) 476, 709
EciI GGCGGA 1 cut(s) 236
Eco130I CCWWGG 1 cut(s) 130
Eco32I GATATC 1 cut(s) 529
Eco47I GGWCC 3 cut(s) 134, 503, 515
Eco57I CTGAAG 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 515
EcoRII CCWGG 2 cut(s) 195, 511
EcoRV GATATC 1 cut(s) 529
EcoT14I CCWWGG 1 cut(s) 130
EcoT22I ATGCAT 1 cut(s) 738
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 1 cut(s) 740
FaiI YATR 8 cut(s) 534, 549, 605, 616, 621, 734, 738, 748
FalI AAGNNNNNCTT 6 cut(s) 77, 109, 371, 403, 432, 464
FaqI GGGAC 1 cut(s) 154
FatI CATG 1 cut(s) 736
FauI CCCGC 1 cut(s) 303
Fnu4HI GCNGC 2 cut(s) 261, 676
FokI GGATG 1 cut(s) 137
Fsp4HI GCNGC 2 cut(s) 261, 676
GluI GCNGC 2 cut(s) 261, 676
GsaI CCCAGC 1 cut(s) 159
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 1 cut(s) 200
HgaI GACGC 2 cut(s) 271, 687
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 1 cut(s) 740
HinfI GANTC 4 cut(s) 40, 121, 413, 631
HphI GGTGA 1 cut(s) 338
Hpy188I TCNGA 3 cut(s) 138, 211, 369
Hpy188III TCNNGA 4 cut(s) 125, 430, 498, 648
Hpy99I CGWCG 2 cut(s) 215, 218
HpyAV CCTTC 4 cut(s) 4, 250, 331, 528
HpyCH4III ACNGT 2 cut(s) 109, 401
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 7 cut(s) 105, 114, 338, 471, 665, 685, 736
HpyF10VI GCNNNNNNNGC 4 cut(s) 111, 279, 288, 449
HpyF3I CTNAG 1 cut(s) 453
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 1 cut(s) 740
Kzo9I GATC 1 cut(s) 20
LmnI GCTCC 1 cut(s) 323
Lsp1109I GCAGC 1 cut(s) 662
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 1 cut(s) 691
MalI GATC 1 cut(s) 22
MbiI CCGCTC 2 cut(s) 263, 310
MboI GATC 1 cut(s) 20
MboII GAAGA 8 cut(s) 91, 94, 214, 277, 382, 475, 493, 696
MluCI AATT 5 cut(s) 339, 373, 425, 552, 712
MmeI TCCRAC 2 cut(s) 87, 234
MnlI CCTC 9 cut(s) 182, 194, 236, 254, 260, 284, 398, 494, 712
Mph1103I ATGCAT 1 cut(s) 738
MroXI GAANNNNTTC 2 cut(s) 383, 412
MseI TTAA 3 cut(s) 342, 711, 725
MslI CAYNNNNRTG 2 cut(s) 74, 624
MspR9I CCNGG 2 cut(s) 197, 513
MvaI CCWGG 2 cut(s) 197, 513
MwoI GCNNNNNNNGC 4 cut(s) 111, 279, 288, 449
NdeII GATC 1 cut(s) 20
NlaIII CATG 1 cut(s) 740
NsiI ATGCAT 1 cut(s) 738
NspI RCATGY 1 cut(s) 740
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PdmI GAANNNNTTC 2 cut(s) 383, 412
PfeI GAWTC 4 cut(s) 40, 121, 413, 631
PkrI GCNGC 2 cut(s) 262, 677
PpuMI RGGWCCY 1 cut(s) 515
PshAI GACNNNNGTC 1 cut(s) 132
PshBI ATTAAT 2 cut(s) 342, 711
Psp5II RGGWCCY 1 cut(s) 515
Psp6I CCWGG 2 cut(s) 195, 511
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 2 cut(s) 195, 511
PspPI GGNCC 3 cut(s) 134, 503, 515
PspPPI RGGWCCY 1 cut(s) 515
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
RseI CAYNNNNRTG 2 cut(s) 74, 624
SaqAI TTAA 3 cut(s) 342, 711, 725
SatI GCNGC 2 cut(s) 261, 676
Sau3AI GATC 1 cut(s) 20
Sau96I GGNCC 3 cut(s) 134, 503, 515
ScrFI CCNGG 2 cut(s) 197, 513
SinI GGWCC 3 cut(s) 134, 503, 515
SmiMI CAYNNNNRTG 2 cut(s) 74, 624
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
Sse9I AATT 5 cut(s) 339, 373, 425, 552, 712
SsiI CCGC 4 cut(s) 247, 261, 271, 310
StyD4I CCNGG 2 cut(s) 195, 511
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 2 cut(s) 109, 401
TaiI ACGT 1 cut(s) 216
TaqI TCGA 4 cut(s) 96, 252, 297, 420
TasI AATT 5 cut(s) 339, 373, 425, 552, 712
TatI WGTACW 1 cut(s) 147
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 4 cut(s) 40, 121, 413, 631
Tru1I TTAA 3 cut(s) 342, 711, 725
Tru9I TTAA 3 cut(s) 342, 711, 725
TseI GCWGC 1 cut(s) 675
TspDTI ATGAA 1 cut(s) 92
VpaK11BI GGWCC 3 cut(s) 134, 503, 515
VspI ATTAAT 2 cut(s) 342, 711
XapI RAATTY 3 cut(s) 373, 425, 552
XceI RCATGY 1 cut(s) 740
XmnI GAANNNNTTC 2 cut(s) 383, 412
ZraI GACGTC 1 cut(s) 214
Zsp2I ATGCAT 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.