MD06G1141400.v1.1

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007112
Physical Location & Seq
Reverse (-)
28501273 .. 28504547
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1141400.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGGAGGAGAAGAGGAAAGAGATCACGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGTACGAGTTCGAGTACTGCGTGGTTTGCAAGCTCAATCACGACCAAGGTCCGAGCCACAGGTACATCCCGAACCACACCAAGTCCCTCTCCGCCTTCCTTTCTCGCTTCCAGTCCAAGCTCGCCGACGTCCGCTTCTTTCTCAAAAACCCTAGCCCTCTCCGCCCCAAACACGCCTCCTGCAATCGCCTCTGGTGCGTCTTCTGCGACTCCGATATCGGCGAGCTCGATAGCTCCTTCGCCTGTGGTAATGCGATTAATCACCTTGCGACCGCTGAACATCTGAAGAATTTGAAGCATTTCTTGTGGAAATACGGTGGAGGAATGGACCGCATCGATACATTTAGAATTTCGGAAAATGAACTTGCTAAGTGGGAGAAGAAGTGTAATTCACTGAAGCTTGAAGCTGTGCCTTGTGGTGAAGGCTCTCGAGAACCGACATTTGGACCTTCGAATGATATCCATATTGAACTGAAATATGGAATTATAGATACTTCTGAAAATAATAGTAATTCATGTTTTTCACATGGTGTCATTCCTTTACCATATCATACAAATGAGAATCAGGTATCCCATTCAGGACTCCCTCAAGTTACAAATGTTGGCTGCTTTCCGCAAGATGTTGCTAATGCTTCTTATCATGGGGGAATACGGTCTGGTACAAATTCCATAAATTCAAATGGTTTAACAGTTAGCAGGAATACCCAACATCCTTTGGTTTCTAATGGTAGAAAATTCTCAGCTGATGGTTACTTCGATAATGAAAGGATAAGAGAGGGGTCTCAGCATGGAAGAATGACAAAAGGGCAGAGCAGTTCTCCAGGTTTTCAGAATCTCACTCAAATATCATCCTTGGGTCCTAAAGAGGCTAGTGGAAATGTGCATACTGGGGCACCTCCTCCTTGGCTTCAAGCAGATGAAGAAATTCAGTTTAGTGTTCCACTGAAACCAGTTTCAGGTAGTCTTATCTCCCTGTCAAATAAGTCGGAGAAGTCCAAGAAATTGAATCCAAACCGTGTCGGAGCAGCTTGGGCAGAAAGGAGAAAGAGGGAGGTGGAGATGGAAAAAAGAGGGGAAATTGTCATGAGTGACTGTGATGCCAATTGGCTCACTAATTTTGGTAGTTTGGCAGTCCGGTAG

Protein Analysis

399

Amino Acids

44.49

Weight (kDa)

9.27

Isoelectric Point (pI)

34.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 25 - 394 2.3e-80 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccB1I GGYRCC 1 cut(s) 949
AciI CCGC 6 cut(s) 150, 190, 220, 330, 388, 671
AcsI RAATTY 6 cut(s) 346, 405, 721, 730, 791, 981
AcuI CTGAAG 2 cut(s) 362, 473
AcyI GRCGYC 1 cut(s) 186
AdeI CACNNNGTG 1 cut(s) 587
AfaI GTAC 4 cut(s) 62, 74, 122, 718
AfiI CCNNNNNNNGG 2 cut(s) 500, 1013
AgsI TTSAA 6 cut(s) 352, 461, 527, 735, 968, 1063
AjnI CCWGG 1 cut(s) 877
AjuI GAANNNNNNNTTGG 2 cut(s) 483, 515
AluBI AGCT 8 cut(s) 91, 178, 283, 291, 457, 464, 800, 1085
AluI AGCT 8 cut(s) 91, 178, 283, 291, 457, 464, 800, 1085
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 843
Ama87I CYCGRG 1 cut(s) 486
ApeKI GCWGC 2 cut(s) 663, 1082
ApoI RAATTY 6 cut(s) 346, 405, 721, 730, 791, 981
AseI ATTAAT 1 cut(s) 315
Asp700I GAANNNNTTC 2 cut(s) 356, 981
AspS9I GGNCC 4 cut(s) 107, 385, 503, 914
AsuHPI GGTGA 2 cut(s) 311, 488
AsuII TTCGAA 1 cut(s) 509
AvaI CYCGRG 1 cut(s) 486
AvaII GGWCC 4 cut(s) 107, 385, 503, 914
BaeGI GKGCMC 1 cut(s) 952
BanI GGYRCC 1 cut(s) 949
BanII GRGCYC 1 cut(s) 285
BarI GAAGNNNNNNTAC 2 cut(s) 44, 76
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 650, 1094
BccI CCATC 2 cut(s) 797, 1111
BciT130I CCWGG 1 cut(s) 879
BciVI GTATCC 1 cut(s) 637
BcoDI GTCTC 1 cut(s) 843
BfaI CTAG 2 cut(s) 210, 927
BfuI GTATCC 1 cut(s) 637
BisI GCNGC 2 cut(s) 664, 1083
BlsI GCNGC 2 cut(s) 665, 1084
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 1 cut(s) 879
Bme18I GGWCC 4 cut(s) 107, 385, 503, 914
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 4 cut(s) 107, 385, 503, 914
BmiI GGNNCC 2 cut(s) 915, 951
BmrFI CCNGG 1 cut(s) 879
BmrI ACTGGG 1 cut(s) 954
BmsI GCATC 2 cut(s) 399, 1144
BmuI ACTGGG 1 cut(s) 954
BoxI GACNNNNGTC 1 cut(s) 105
BpiI GAAGAC 1 cut(s) 250
BplI GAGNNNNNCTC 2 cut(s) 859, 891
BpmI CTGGAG 1 cut(s) 861
Bpu14I TTCGAA 1 cut(s) 509
BpuEI CTTGAG 1 cut(s) 630
Bsa29I ATCGAT 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 843
BsaJI CCNNGG 3 cut(s) 103, 909, 959
BsaWI WCCGGW 1 cut(s) 1191
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 2 cut(s) 500, 1013
Bse1I ACTGG 3 cut(s) 169, 949, 1007
BseBI CCWGG 1 cut(s) 879
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 3 cut(s) 103, 909, 959
BseGI GGATG 3 cut(s) 123, 766, 905
BseLI CCNNNNNNNGG 2 cut(s) 500, 1013
BseMII CTCAG 2 cut(s) 810, 854
BseNI ACTGG 3 cut(s) 169, 949, 1007
BseRI GAGGAG 2 cut(s) 20, 945
BseSI GKGCMC 1 cut(s) 952
BseXI GCAGC 2 cut(s) 650, 1094
Bsh1285I CGRYCG 1 cut(s) 330
BshNI GGYRCC 1 cut(s) 949
BshVI ATCGAT 1 cut(s) 393
BsiEI CGRYCG 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 1 cut(s) 1192
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 500, 1013
BsmAI GTCTC 1 cut(s) 843
BsmFI GGGAC 1 cut(s) 127
Bso31I GGTCTC 1 cut(s) 843
BsoBI CYCGRG 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 285, 952
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 6 cut(s) 150, 190, 220, 330, 388, 671
BspCNI CTCAG 2 cut(s) 809, 853
BspDI ATCGAT 1 cut(s) 393
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 2 cut(s) 915, 951
BspT104I TTCGAA 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 949
BspTNI GGTCTC 1 cut(s) 843
BsrI ACTGG 3 cut(s) 169, 949, 1007
BssECI CCNNGG 3 cut(s) 103, 909, 959
BssMI GATC 1 cut(s) 21
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 3 cut(s) 103, 909, 959
Bst2UI CCWGG 1 cut(s) 879
Bst4CI ACNGT 5 cut(s) 374, 711, 748, 1073, 1151
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 89, 180, 281
BstDEI CTNAG 3 cut(s) 426, 796, 840
BstF5I GGATG 3 cut(s) 123, 766, 905
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 1 cut(s) 843
BstMBI GATC 1 cut(s) 21
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 5 cut(s) 84, 219, 252, 261, 1088
BstNI CCWGG 1 cut(s) 879
BstPAI GACNNNNGTC 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 877
BstSLI GKGCMC 1 cut(s) 952
BstV1I GCAGC 2 cut(s) 650, 1094
BstV2I GAAGAC 1 cut(s) 250
Bsu15I ATCGAT 1 cut(s) 393
BsuI GTATCC 1 cut(s) 637
BsuTUI ATCGAT 1 cut(s) 393
BtsCI GGATG 3 cut(s) 123, 766, 905
BtsIMutI CAGTG 2 cut(s) 449, 998
Cac8I GCNNGC 3 cut(s) 89, 180, 281
CciI TCATGA 1 cut(s) 1140
Cfr13I GGNCC 4 cut(s) 107, 385, 503, 914
ClaI ATCGAT 1 cut(s) 393
CseI GACGC 1 cut(s) 244
Csp6I GTAC 4 cut(s) 61, 73, 121, 717
CviAII CATG 5 cut(s) 573, 584, 698, 845, 1141
CviQI GTAC 4 cut(s) 61, 73, 121, 717
DdeI CTNAG 3 cut(s) 426, 796, 840
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
DraIII CACNNNGTG 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 5
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 2 cut(s) 139, 209
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 3 cut(s) 103, 909, 959
Eco24I GRGCYC 1 cut(s) 285
Eco31I GGTCTC 1 cut(s) 843
Eco32I GATATC 2 cut(s) 274, 517
Eco47I GGWCC 4 cut(s) 107, 385, 503, 914
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 2 cut(s) 362, 473
Eco88I CYCGRG 1 cut(s) 486
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 914
EcoRII CCWGG 1 cut(s) 877
EcoRV GATATC 2 cut(s) 274, 517
EcoT14I CCWWGG 3 cut(s) 103, 909, 959
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 3 cut(s) 103, 909, 959
FaeI CATG 5 cut(s) 576, 587, 701, 848, 1144
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FaqI GGGAC 1 cut(s) 127
FatI CATG 5 cut(s) 572, 583, 697, 844, 1140
Fnu4HI GCNGC 2 cut(s) 664, 1083
FokI GGATG 3 cut(s) 110, 753, 892
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 2 cut(s) 664, 1083
FspBI CTAG 2 cut(s) 210, 927
GluI GCNGC 2 cut(s) 664, 1083
GsuI CTGGAG 1 cut(s) 861
HapII CCGG 1 cut(s) 1192
HgaI GACGC 1 cut(s) 244
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 5 cut(s) 576, 587, 701, 848, 1144
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 5 cut(s) 266, 619, 639, 889, 1063
HpaII CCGG 1 cut(s) 1192
HphI GGTGA 2 cut(s) 311, 488
Hpy188I TCNGA 8 cut(s) 111, 271, 342, 412, 556, 888, 1045, 1079
Hpy188III TCNNGA 7 cut(s) 25, 98, 127, 486, 488, 636, 1141
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 4 cut(s) 163, 304, 473, 516
HpyCH4III ACNGT 5 cut(s) 374, 711, 748, 1073, 1151
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 3 cut(s) 87, 240, 940
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 219, 252, 261, 1088
HpyF3I CTNAG 3 cut(s) 426, 796, 840
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 5 cut(s) 576, 587, 701, 848, 1144
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 2 cut(s) 296, 1079
Lsp1109I GCAGC 2 cut(s) 650, 1094
LweI GCATC 2 cut(s) 399, 1144
MaeI CTAG 2 cut(s) 210, 927
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 3 cut(s) 649, 806, 1145
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MfeI CAATTG 1 cut(s) 1159
MhlI GDGCHC 2 cut(s) 285, 952
MlyI GAGTC 2 cut(s) 260, 633
MmeI TCCRAC 2 cut(s) 1023, 1057
MroXI GAANNNNTTC 2 cut(s) 356, 981
MseI TTAA 2 cut(s) 315, 743
MslI CAYNNNNRTG 1 cut(s) 612
MspA1I CMGCKG 2 cut(s) 332, 800
MspI CCGG 1 cut(s) 1192
MspR9I CCNGG 1 cut(s) 879
MunI CAATTG 1 cut(s) 1159
MvaI CCWGG 1 cut(s) 879
MwoI GCNNNNNNNGC 5 cut(s) 84, 219, 252, 261, 1088
NdeII GATC 1 cut(s) 21
NlaIII CATG 5 cut(s) 576, 587, 701, 848, 1144
NlaIV GGNNCC 2 cut(s) 915, 951
NmuCI GTSAC 1 cut(s) 1145
NspV TTCGAA 1 cut(s) 509
PaeR7I CTCGAG 1 cut(s) 486
PagI TCATGA 1 cut(s) 1140
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 282
PdmI GAANNNNTTC 2 cut(s) 356, 981
PfeI GAWTC 3 cut(s) 619, 889, 1063
PkrI GCNGC 2 cut(s) 665, 1084
PleI GAGTC 2 cut(s) 260, 633
PpsI GAGTC 2 cut(s) 260, 633
PpuMI RGGWCCY 1 cut(s) 914
PshAI GACNNNNGTC 1 cut(s) 105
PshBI ATTAAT 1 cut(s) 315
Psp124BI GAGCTC 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 914
Psp6I CCWGG 1 cut(s) 877
PspGI CCWGG 1 cut(s) 877
PspN4I GGNNCC 2 cut(s) 915, 951
PspPI GGNCC 4 cut(s) 107, 385, 503, 914
PspPPI RGGWCCY 1 cut(s) 914
PvuII CAGCTG 1 cut(s) 800
RsaI GTAC 4 cut(s) 62, 74, 122, 718
RsaNI GTAC 4 cut(s) 61, 73, 121, 717
RseI CAYNNNNRTG 1 cut(s) 612
SacI GAGCTC 1 cut(s) 285
SaqAI TTAA 2 cut(s) 315, 743
SatI GCNGC 2 cut(s) 664, 1083
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 4 cut(s) 107, 385, 503, 914
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 2 cut(s) 260, 633
ScrFI CCNGG 1 cut(s) 879
SduI GDGCHC 2 cut(s) 285, 952
SfaNI GCATC 2 cut(s) 399, 1144
Sfr274I CTCGAG 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 509
SinI GGWCC 4 cut(s) 107, 385, 503, 914
SlaI CTCGAG 1 cut(s) 486
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 2 cut(s) 486, 645
SmoI CTYRAG 2 cut(s) 486, 645
SsiI CCGC 6 cut(s) 150, 190, 220, 330, 388, 671
SspMI CTAG 2 cut(s) 210, 927
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 877
StyI CCWWGG 3 cut(s) 103, 909, 959
TaaI ACNGT 5 cut(s) 374, 711, 748, 1073, 1151
TaiI ACGT 1 cut(s) 189
TaqI TCGA 6 cut(s) 69, 285, 393, 487, 509, 813
TatI WGTACW 1 cut(s) 72
TfiI GAWTC 3 cut(s) 619, 889, 1063
Tru1I TTAA 2 cut(s) 315, 743
Tru9I TTAA 2 cut(s) 315, 743
TscAI CASTG 2 cut(s) 456, 1005
TseFI GTSAC 1 cut(s) 1145
TseI GCWGC 2 cut(s) 663, 1082
Tsp45I GTSAC 1 cut(s) 1145
TspDTI ATGAA 4 cut(s) 432, 561, 834, 990
TspRI CASTG 2 cut(s) 456, 1005
VpaK11BI GGWCC 4 cut(s) 107, 385, 503, 914
VspI ATTAAT 1 cut(s) 315
XapI RAATTY 6 cut(s) 346, 405, 721, 730, 791, 981
XhoI CTCGAG 1 cut(s) 486
XmnI GAANNNNTTC 2 cut(s) 356, 981
XspI CTAG 2 cut(s) 210, 927
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.