MD14G1156600.v1.1

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007112
Physical Location & Seq
Reverse (-)
25095415 .. 25098278
2864 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1156600.v1.1.491

Sequence Viewer

Length: 1260 bp
ATGCAGGAGAAGAAGAAGAAGAAGTACGAGTTCGAGTACTGCGTGGTTTGCAAGCTGAATCACGACCAAGGTCCGAGCCACAAGTACATACCCAGCCACACCAAGTCCCTCTCCGCCTTCCTCACTCGCTTCCAAGCCAAGCTCTCCGATGTCCGCTTCTTTCTCAAAAACCCTATCCCTCTCCGCCCCGAACACGCCTCCCGCAACCGCCTCTGGTGCGTTTTCTGCGACTCCGACATCGACGAGCTCGATAGCTCCTTCGCCTGTGGTAATGCAATTAATCACCTTGCGAACGTGGAACATCTGAAGAATTTGAAGCATTTCTTGTGGAAATACGGTGGAGGAATGGACCGTGTCGATACATTCAGAATTTCGGAAGATGAACTTGCTAAGTGGGAGAAGAAGTGTAATTCATTGAAGCTTGAAGCTGTGCCATGTGGTGAAGGATCTCAAGGACCGACATTTGGACCATCGAATGATATCCATACTGAACTGAAATACGGAATTATAGATACTTTTGAAAATAATAGTAGTTCAAGTTTTTCACATGGTGTTATGCCTTTACTATATCATACAAATGAGTATCAGGTATCCCATTCAGGACTCCCTCAAGTTACAAATGTTGGCTGCTTTTCGCAAGATGTTGCTACCTCTTCTTATCATGGGGGATCAAGTTCTGTTAGCAGGAGTAACCAACATTCTTTGGTTTCTAATGGTAGAAAATCCTCGGCTGATGGTTACTTCAGTAATGAAAGGATGAGAGAGGGGTCTCAGCATGAAAGAATGACAAACGAGCAGAGCAGTTCTCCAGTTTTTCAGAATCTCACCCAAATATCGTCCATGGGTCCTAAAGAGGCTAGAGGAAATGTGCGCACTGGGGCACCTCCTCCTTGGCTTCAAGCAGATGAAGAAATTCAGTGTAGTGTTCCACTGAAACGAGTTTCAGGTAGCCTTGTTCCCCCGTCAAATAAATCGGGGAAGTCCAAGAAATTGAACCCAAACCGTGTGGGAGCAGCTTGGGCAGAAAGGAGAAAGAGGGAGATGGAGATGGAGAAAAGAGGGGAAATTGTCATGAGTGACTGCGATGCCAGTTGGCTCCCTAATTTTGGTAGAGTTTGGCAGTCCGGTAGCCGAAAAGAGTCCAGAAAAGAATTTGAGTTGGAGAAACAAAAATTACCCAAGGTTGAAAGTCAGTCGGATATGCCAATTAAGATACAGCCTTACATCAGCAAACGAATGAGAACTGATTCGAGTCAATAA

Protein Analysis

420

Amino Acids

47.36

Weight (kDa)

9.0

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 13 - 391 1.6e-95 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 872
AccB1I GGYRCC 1 cut(s) 880
AciI CCGC 5 cut(s) 114, 154, 184, 202, 208
AclWI GGATC 2 cut(s) 454, 676
AcsI RAATTY 4 cut(s) 310, 369, 912, 1151
AcuI CTGAAG 2 cut(s) 326, 727
AdeI CACNNNGTG 1 cut(s) 551
AfaI GTAC 3 cut(s) 26, 38, 86
AfiI CCNNNNNNNGG 2 cut(s) 464, 1106
AgsI TTSAA 8 cut(s) 316, 418, 425, 521, 537, 899, 994, 1187
AluBI AGCT 7 cut(s) 55, 142, 247, 255, 421, 428, 1016
AluI AGCT 7 cut(s) 55, 142, 247, 255, 421, 428, 1016
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 774
AlwI GGATC 2 cut(s) 454, 676
ApeKI GCWGC 2 cut(s) 627, 1013
ApoI RAATTY 4 cut(s) 310, 369, 912, 1151
AseI ATTAAT 1 cut(s) 279
Asp700I GAANNNNTTC 3 cut(s) 320, 912, 1246
AspLEI GCGC 1 cut(s) 873
AspS9I GGNCC 5 cut(s) 71, 349, 455, 467, 845
AsuHPI GGTGA 3 cut(s) 275, 452, 817
AvaII GGWCC 5 cut(s) 71, 349, 455, 467, 845
BaeGI GKGCMC 1 cut(s) 883
BanI GGYRCC 1 cut(s) 880
BanII GRGCYC 1 cut(s) 249
BarI GAAGNNNNNNTAC 2 cut(s) 8, 40
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 614, 1025
BccI CCATC 4 cut(s) 478, 728, 1036, 1042
BciVI GTATCC 1 cut(s) 601
BcoDI GTCTC 1 cut(s) 774
BfaI CTAG 1 cut(s) 858
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 2 cut(s) 628, 1014
BlsI GCNGC 2 cut(s) 629, 1015
BmcAI AGTACT 1 cut(s) 38
Bme18I GGWCC 5 cut(s) 71, 349, 455, 467, 845
BmgT120I GGNCC 5 cut(s) 71, 349, 455, 467, 845
BmiI GGNNCC 3 cut(s) 846, 882, 1097
BmrI ACTGGG 1 cut(s) 885
BmsI GCATC 1 cut(s) 1075
BmuI ACTGGG 1 cut(s) 885
BoxI GACNNNNGTC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 790, 822
BpmI CTGGAG 1 cut(s) 792
BpuEI CTTGAG 2 cut(s) 435, 594
BsaI GGTCTC 1 cut(s) 774
BsaJI CCNNGG 5 cut(s) 67, 726, 840, 890, 1179
BsaWI WCCGGW 1 cut(s) 1124
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 1106
Bse1I ACTGG 3 cut(s) 809, 880, 1089
BseDI CCNNGG 5 cut(s) 67, 726, 840, 890, 1179
BseGI GGATG 1 cut(s) 762
BseLI CCNNNNNNNGG 2 cut(s) 464, 1106
BseMII CTCAG 1 cut(s) 785
BseNI ACTGG 3 cut(s) 809, 880, 1089
BseRI GAGGAG 1 cut(s) 876
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 2 cut(s) 614, 1025
BseYI CCCAGC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 880
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 1 cut(s) 1125
BslFI GGGAC 1 cut(s) 91
BslI CCNNNNNNNGG 2 cut(s) 464, 1106
BsmAI GTCTC 1 cut(s) 774
BsmFI GGGAC 1 cut(s) 91
Bso31I GGTCTC 1 cut(s) 774
Bsp1286I GDGCHC 2 cut(s) 249, 883
Bsp143I GATC 2 cut(s) 446, 668
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 5 cut(s) 114, 154, 184, 202, 208
BspCNI CTCAG 1 cut(s) 784
BspHI TCATGA 1 cut(s) 1071
BspLI GGNNCC 3 cut(s) 846, 882, 1097
BspPI GGATC 2 cut(s) 454, 676
BspT107I GGYRCC 1 cut(s) 880
BspTNI GGTCTC 1 cut(s) 774
BsrI ACTGG 3 cut(s) 809, 880, 1089
BssECI CCNNGG 5 cut(s) 67, 726, 840, 890, 1179
BssMI GATC 2 cut(s) 446, 668
BssT1I CCWWGG 4 cut(s) 67, 840, 890, 1179
Bst4CI ACNGT 3 cut(s) 338, 353, 1004
Bst6I CTCTTC 1 cut(s) 658
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 2 cut(s) 390, 771
BstDSI CCRYGG 1 cut(s) 840
BstF5I GGATG 1 cut(s) 762
BstHHI GCGC 1 cut(s) 873
BstKTI GATC 2 cut(s) 449, 671
BstMAI GTCTC 1 cut(s) 774
BstMBI GATC 2 cut(s) 446, 668
BstMWI GCNNNNNNNGC 4 cut(s) 48, 216, 225, 1019
BstPAI GACNNNNGTC 1 cut(s) 69
BstSLI GKGCMC 1 cut(s) 883
BstV1I GCAGC 2 cut(s) 614, 1025
BstX2I RGATCY 1 cut(s) 446
BstYI RGATCY 1 cut(s) 446
BsuI GTATCC 1 cut(s) 601
BtgI CCRYGG 1 cut(s) 840
BtgZI GCGATG 1 cut(s) 1098
BtsCI GGATG 1 cut(s) 762
BtsIMutI CAGTG 3 cut(s) 873, 923, 929
Cac8I GCNNGC 1 cut(s) 53
CciI TCATGA 1 cut(s) 1071
CfoI GCGC 1 cut(s) 873
Cfr13I GGNCC 5 cut(s) 71, 349, 455, 467, 845
Csp6I GTAC 3 cut(s) 25, 37, 85
CspCI CAANNNNNGTGG 2 cut(s) 987, 1022
CviAII CATG 6 cut(s) 435, 548, 662, 776, 841, 1072
CviQI GTAC 3 cut(s) 25, 37, 85
DdeI CTNAG 2 cut(s) 390, 771
DpnI GATC 2 cut(s) 448, 670
DpnII GATC 2 cut(s) 446, 668
DraIII CACNNNGTG 1 cut(s) 551
Eam1104I CTCTTC 1 cut(s) 658
EarI CTCTTC 1 cut(s) 658
EciI GGCGGA 2 cut(s) 103, 173
Ecl136II GAGCTC 1 cut(s) 247
Eco130I CCWWGG 4 cut(s) 67, 840, 890, 1179
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 774
Eco32I GATATC 1 cut(s) 481
Eco47I GGWCC 5 cut(s) 71, 349, 455, 467, 845
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 326, 727
EcoICRI GAGCTC 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 845
EcoRV GATATC 1 cut(s) 481
EcoT14I CCWWGG 4 cut(s) 67, 840, 890, 1179
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 4 cut(s) 67, 840, 890, 1179
FaeI CATG 6 cut(s) 438, 551, 665, 779, 844, 1075
FalI AAGNNNNNCTT 4 cut(s) 308, 340, 369, 401
FaqI GGGAC 1 cut(s) 91
FatI CATG 6 cut(s) 434, 547, 661, 775, 840, 1071
FauI CCCGC 1 cut(s) 209
Fnu4HI GCNGC 2 cut(s) 628, 1014
FokI GGATG 1 cut(s) 769
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 2 cut(s) 628, 1014
FspAI RTGCGCAY 1 cut(s) 872
FspBI CTAG 1 cut(s) 858
FspI TGCGCA 1 cut(s) 872
GlaI GCGC 1 cut(s) 872
GluI GCNGC 2 cut(s) 628, 1014
GsaI CCCAGC 1 cut(s) 96
GsuI CTGGAG 1 cut(s) 792
HapII CCGG 1 cut(s) 1125
HhaI GCGC 1 cut(s) 873
Hin1II CATG 6 cut(s) 438, 551, 665, 779, 844, 1075
Hin6I GCGC 1 cut(s) 871
HinP1I GCGC 1 cut(s) 871
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 7 cut(s) 58, 230, 603, 820, 1139, 1247, 1252
HpaII CCGG 1 cut(s) 1125
HphI GGTGA 3 cut(s) 275, 452, 817
Hpy188I TCNGA 8 cut(s) 75, 148, 235, 306, 368, 376, 819, 1198
Hpy188III TCNNGA 4 cut(s) 62, 600, 1072, 1143
Hpy99I CGWCG 1 cut(s) 245
HpyAV CCTTC 3 cut(s) 127, 268, 437
HpyCH4III ACNGT 3 cut(s) 338, 353, 1004
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 3 cut(s) 4, 51, 275
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 216, 225, 1019
HpyF3I CTNAG 2 cut(s) 390, 771
HpySE526I ACGT 1 cut(s) 294
Hsp92II CATG 6 cut(s) 438, 551, 665, 779, 844, 1075
HspAI GCGC 1 cut(s) 871
Kzo9I GATC 2 cut(s) 446, 668
LmnI GCTCC 3 cut(s) 260, 1010, 1101
Lsp1109I GCAGC 2 cut(s) 614, 1025
LweI GCATC 1 cut(s) 1075
MaeI CTAG 1 cut(s) 858
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 4 cut(s) 613, 689, 737, 1076
MalI GATC 2 cut(s) 448, 670
MboI GATC 2 cut(s) 446, 668
MboII GAAGA 9 cut(s) 22, 25, 28, 31, 319, 389, 412, 645, 920
MflI RGATCY 1 cut(s) 446
MhlI GDGCHC 2 cut(s) 249, 883
MlyI GAGTC 3 cut(s) 224, 597, 1148
MmeI TCCRAC 3 cut(s) 258, 1140, 1176
MroXI GAANNNNTTC 3 cut(s) 320, 912, 1246
MseI TTAA 2 cut(s) 279, 1209
MslI CAYNNNNRTG 1 cut(s) 576
MspI CCGG 1 cut(s) 1125
MwoI GCNNNNNNNGC 4 cut(s) 48, 216, 225, 1019
NcoI CCATGG 1 cut(s) 840
NdeII GATC 2 cut(s) 446, 668
NlaIII CATG 6 cut(s) 438, 551, 665, 779, 844, 1075
NlaIV GGNNCC 3 cut(s) 846, 882, 1097
NmeAIII GCCGAG 1 cut(s) 707
NmuCI GTSAC 1 cut(s) 1076
NsbI TGCGCA 1 cut(s) 872
PagI TCATGA 1 cut(s) 1071
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 246
PdmI GAANNNNTTC 3 cut(s) 320, 912, 1246
PfeI GAWTC 3 cut(s) 58, 820, 1247
PflFI GACNNNGTC 1 cut(s) 353
PkrI GCNGC 2 cut(s) 629, 1015
PleI GAGTC 3 cut(s) 224, 597, 1147
PpsI GAGTC 3 cut(s) 224, 597, 1147
PpuMI RGGWCCY 1 cut(s) 845
PshAI GACNNNNGTC 1 cut(s) 69
PshBI ATTAAT 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 249
Psp5II RGGWCCY 1 cut(s) 845
PspFI CCCAGC 1 cut(s) 92
PspN4I GGNNCC 3 cut(s) 846, 882, 1097
PspPI GGNCC 5 cut(s) 71, 349, 455, 467, 845
PspPPI RGGWCCY 1 cut(s) 845
PsuI RGATCY 1 cut(s) 446
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 3 cut(s) 26, 38, 86
RsaNI GTAC 3 cut(s) 25, 37, 85
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 279, 1209
SatI GCNGC 2 cut(s) 628, 1014
Sau3AI GATC 2 cut(s) 446, 668
Sau96I GGNCC 5 cut(s) 71, 349, 455, 467, 845
ScaI AGTACT 1 cut(s) 38
SchI GAGTC 3 cut(s) 224, 597, 1148
SduI GDGCHC 2 cut(s) 249, 883
SfaNI GCATC 1 cut(s) 1075
SinI GGWCC 5 cut(s) 71, 349, 455, 467, 845
SmiMI CAYNNNNRTG 1 cut(s) 576
SmlI CTYRAG 2 cut(s) 450, 609
SmoI CTYRAG 2 cut(s) 450, 609
SsiI CCGC 5 cut(s) 114, 154, 184, 202, 208
SspMI CTAG 1 cut(s) 858
SstI GAGCTC 1 cut(s) 249
StyI CCWWGG 4 cut(s) 67, 840, 890, 1179
TaaI ACNGT 3 cut(s) 338, 353, 1004
TaiI ACGT 1 cut(s) 297
TaqI TCGA 6 cut(s) 33, 240, 249, 357, 473, 1250
TaqII GACCGA 1 cut(s) 472
TatI WGTACW 2 cut(s) 36, 84
TfiI GAWTC 3 cut(s) 58, 820, 1247
Tru1I TTAA 2 cut(s) 279, 1209
Tru9I TTAA 2 cut(s) 279, 1209
TscAI CASTG 3 cut(s) 880, 923, 936
TseFI GTSAC 1 cut(s) 1076
TseI GCWGC 2 cut(s) 627, 1013
Tsp45I GTSAC 1 cut(s) 1076
TspDTI ATGAA 5 cut(s) 396, 402, 765, 792, 921
TspGWI ACGGA 1 cut(s) 516
TspRI CASTG 3 cut(s) 880, 923, 936
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 5 cut(s) 71, 349, 455, 467, 845
VspI ATTAAT 1 cut(s) 279
XapI RAATTY 4 cut(s) 310, 369, 912, 1151
XmnI GAANNNNTTC 3 cut(s) 320, 912, 1246
XspI CTAG 1 cut(s) 858
ZrmI AGTACT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.