FvH4_5g08360

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007112
Physical Location & Seq
Forward (+)
4862685 .. 4866008
3324 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08360.t1

Sequence Viewer

Length: 1248 bp
ATGAAGAAGACGAAGACGAAGAAGAAGAAGCACGAGTTCGAATATTGCACGGTTTGCAAGCTCAATCACGACCAAGGTCCGAGCCACAAGTACATCCCCAGCCACACCAAGTCCCTCTCTACTTTCCTCTCCCGCTTCCAGGCCAAGCTCTCCGACGTCGTTTTCTTCCTCAACAACCCTACCTTCCTCCGCCTCGAACACGCCGCCCGCAACCGCCTCTGGTGCGTCTTCTGCGATTTCGACATTGACGAGCGCGACAGCTCCTTCGCCTGTGGCAATGCCATTAATCACCTTGCGAGTGCCGAACATCTGAAGAATTTGAAGCATTTTTTGTGGAAATACGGTGGAGGAATGGATTCTGTAGATAAATTCCGGATATTGGAAGCTGACCTTGCTAAGTGGGAGAAAAAGTGCAAGGCATTGAAGACTGAAGCTGTATCTGGAGGACCGATGCCAGGGCCTTCAAATGATATCCATACTAAACTTGAATATGGAATTTTAGATACTTTCCAAAATAGTAGTAGTTCAAAATTTTCAAATGGTGTTATGCCTTTACTAGATCATACAAATGAGAATCAGGTATCCCATTCAGTACCCTCCCAATATGCAAACGGTGGTTGCTTTGTGCAAGATGTTGTTGCTTCCTCTTCTATAAATTCCCAAGGTTTTACAGTTAGTAGCCAGGGTTCTCTTATTTCTAATGGTAGAAAAGGCTCGGCTGATGTCAATTTGAATAATGAAAGGATGAACAAGGTGTACCAGCACGGAAGAATGGCAATGGGACAGAGCAGTTCTCAAGGTGTTCATAGCCTCACTCAAATTCCTTACAAGGGTCCTAAAGATGCTAGTGGAAATGTGCATACTGGAGCACTTCCTCCTTGGTTTGCAGCTGAGGAAGAAATTCAGCTAAGTATTCCACTACAACGAGTTTCTGCTACCCCTCTTCCTCTGTCAAATCAGGCAGGGAAGTCCAAGAAATTGAACCCAAAACGAGTGGGAGCAGCTTGGGCAGAAAGGAGAAAGAGAGAGATGGAGATGGAGCAAAGAGGTGAGATTGTCAAGAACGATTATGATGCTAATTGGCTCCCCAATTTTGGTGGAGTTTGGCAATCTGGTACCCGGAAAGAGGCTAGAAAAGAATTTGAGTTGGAGAAACATAATTTAGTTAAGGTTGAAAGTCAATCTGAGATGCCAATCAAGATACAGCCTTACGTCAGCAAAAGGATGCGAACAGGTGCAAGTGAATAA

Protein Analysis

416

Amino Acids

46.47

Weight (kDa)

9.33

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 15 - 386 9.5e-89 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 159
Acc65I GGTACC 1 cut(s) 1115
AccB1I GGYRCC 1 cut(s) 1115
AccII CGCG 1 cut(s) 255
AccIII TCCGGA 1 cut(s) 372
AciI CCGC 5 cut(s) 133, 190, 204, 208, 214
AcsI RAATTY 8 cut(s) 316, 368, 495, 530, 655, 819, 900, 1139
AcuI CTGAAG 2 cut(s) 332, 450
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 4 cut(s) 92, 594, 758, 1117
AfiI CCNNNNNNNGG 6 cut(s) 139, 379, 455, 830, 1094, 1126
AgsI TTSAA 9 cut(s) 322, 424, 465, 488, 528, 537, 733, 982, 1175
AjnI CCWGG 3 cut(s) 138, 454, 681
AluBI AGCT 8 cut(s) 61, 148, 261, 386, 434, 890, 907, 1004
AluI AGCT 8 cut(s) 61, 148, 261, 386, 434, 890, 907, 1004
Alw21I GWGCWC 1 cut(s) 871
Aor13HI TCCGGA 1 cut(s) 372
AoxI GGCC 2 cut(s) 141, 458
ApeKI GCWGC 2 cut(s) 887, 1001
ApoI RAATTY 8 cut(s) 316, 368, 495, 530, 655, 819, 900, 1139
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
AseI ATTAAT 1 cut(s) 285
Asp700I GAANNNNTTC 2 cut(s) 355, 900
Asp718I GGTACC 1 cut(s) 1115
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 4 cut(s) 77, 446, 458, 833
AsuC2I CCSGG 1 cut(s) 1120
AsuHPI GGTGA 2 cut(s) 281, 1061
AsuII TTCGAA 1 cut(s) 39
AvaII GGWCC 3 cut(s) 77, 446, 833
BanI GGYRCC 1 cut(s) 1115
BauI CACGAG 1 cut(s) 32
BbsI GAAGAC 4 cut(s) 14, 20, 220, 431
Bbv12I GWGCWC 1 cut(s) 871
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 2 cut(s) 899, 1013
BccI CCATC 2 cut(s) 1024, 1030
BcgI CGANNNNNNTGC 2 cut(s) 1055, 1089
BciT130I CCWGG 3 cut(s) 140, 456, 683
BciVI GTATCC 1 cut(s) 592
BcnI CCSGG 1 cut(s) 1120
BfaI CTAG 3 cut(s) 557, 846, 1131
BfmI CTRYAG 1 cut(s) 360
BfuI GTATCC 1 cut(s) 592
BisI GCNGC 3 cut(s) 204, 888, 1002
BlsI GCNGC 3 cut(s) 205, 889, 1003
Bme1390I CCNGG 4 cut(s) 140, 456, 683, 1120
Bme18I GGWCC 3 cut(s) 77, 446, 833
BmgT120I GGNCC 4 cut(s) 77, 446, 458, 833
BmiI GGNNCC 3 cut(s) 834, 1085, 1117
BmrFI CCNGG 4 cut(s) 140, 456, 683, 1120
BmsI GCATC 5 cut(s) 441, 832, 1063, 1179, 1215
BoxI GACNNNNGTC 1 cut(s) 75
BpiI GAAGAC 4 cut(s) 14, 20, 220, 431
BplI GAGNNNNNCTC 2 cut(s) 778, 810
BpmI CTGGAG 2 cut(s) 462, 885
Bpu10I CCTNAGC 1 cut(s) 891
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 780
BpuMI CCSGG 1 cut(s) 1120
BsaBI GATNNNNATC 1 cut(s) 1193
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 5 cut(s) 73, 455, 661, 682, 878
BsaWI WCCGGW 1 cut(s) 372
BsaXI ACNNNNNCTCC 2 cut(s) 395, 425
Bsc4I CCNNNNNNNGG 6 cut(s) 139, 379, 455, 830, 1094, 1126
Bse1I ACTGG 1 cut(s) 868
Bse3DI GCAATG 2 cut(s) 283, 783
Bse8I GATNNNNATC 1 cut(s) 1193
BseAI TCCGGA 1 cut(s) 372
BseBI CCWGG 3 cut(s) 140, 456, 683
BseDI CCNNGG 5 cut(s) 73, 455, 661, 682, 878
BseGI GGATG 3 cut(s) 93, 750, 1230
BseJI GATNNNNATC 1 cut(s) 1193
BseLI CCNNNNNNNGG 6 cut(s) 139, 379, 455, 830, 1094, 1126
BseMI GCAATG 2 cut(s) 283, 783
BseMII CTCAG 2 cut(s) 882, 1176
BseNI ACTGG 1 cut(s) 868
BseXI GCAGC 2 cut(s) 899, 1013
BseYI CCCAGC 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 2 cut(s) 143, 460
BshNI GGYRCC 1 cut(s) 1115
BsiHKAI GWGCWC 1 cut(s) 871
BsiSI CCGG 2 cut(s) 373, 1120
BslFI GGGAC 2 cut(s) 97, 795
BslI CCNNNNNNNGG 6 cut(s) 139, 379, 455, 830, 1094, 1126
BsmFI GGGAC 2 cut(s) 97, 795
BsnI GGCC 2 cut(s) 143, 460
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 871
Bsp13I TCCGGA 1 cut(s) 372
Bsp143I GATC 1 cut(s) 559
BspACI CCGC 5 cut(s) 133, 190, 204, 208, 214
BspANI GGCC 2 cut(s) 143, 460
BspCNI CTCAG 2 cut(s) 883, 1177
BspEI TCCGGA 1 cut(s) 372
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 3 cut(s) 834, 1085, 1117
BspT104I TTCGAA 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 1115
BsrDI GCAATG 2 cut(s) 283, 783
BsrI ACTGG 1 cut(s) 868
BssECI CCNNGG 5 cut(s) 73, 455, 661, 682, 878
BssMI GATC 1 cut(s) 559
BssNI GRCGYC 1 cut(s) 156
BssSI CACGAG 1 cut(s) 32
BssT1I CCWWGG 3 cut(s) 73, 661, 878
Bst2BI CACGAG 1 cut(s) 32
Bst2UI CCWGG 3 cut(s) 140, 456, 683
Bst4CI ACNGT 4 cut(s) 52, 344, 614, 673
Bst6I CTCTTC 2 cut(s) 652, 948
BstACI GRCGYC 1 cut(s) 156
BstAPI GCANNNNNTGC 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 59, 208
BstDEI CTNAG 4 cut(s) 396, 891, 908, 1185
BstF5I GGATG 3 cut(s) 93, 750, 1230
BstFNI CGCG 1 cut(s) 255
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 1 cut(s) 562
BstMBI GATC 1 cut(s) 559
BstMWI GCNNNNNNNGC 5 cut(s) 54, 222, 231, 392, 1007
BstNI CCWGG 3 cut(s) 140, 456, 683
BstPAI GACNNNNGTC 1 cut(s) 75
BstSCI CCNGG 4 cut(s) 138, 454, 681, 1118
BstSFI CTRYAG 1 cut(s) 360
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 2 cut(s) 899, 1013
BstV2I GAAGAC 4 cut(s) 14, 20, 220, 431
BsuI GTATCC 1 cut(s) 592
BsuRI GGCC 2 cut(s) 143, 460
BtsCI GGATG 3 cut(s) 93, 750, 1230
Cac8I GCNNGC 2 cut(s) 59, 208
CfoI GCGC 1 cut(s) 255
Cfr13I GGNCC 4 cut(s) 77, 446, 458, 833
CseI GACGC 1 cut(s) 214
Csp6I GTAC 4 cut(s) 91, 593, 757, 1116
CspCI CAANNNNNGTGG 4 cut(s) 975, 1010, 1078, 1113
CviQI GTAC 4 cut(s) 91, 593, 757, 1116
DdeI CTNAG 4 cut(s) 396, 891, 908, 1185
DpnI GATC 1 cut(s) 561
DpnII GATC 1 cut(s) 559
Eam1104I CTCTTC 2 cut(s) 652, 948
EarI CTCTTC 2 cut(s) 652, 948
EciI GGCGGA 1 cut(s) 179
Eco130I CCWWGG 3 cut(s) 73, 661, 878
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 3 cut(s) 77, 446, 833
Eco57I CTGAAG 2 cut(s) 332, 450
EcoO109I RGGNCCY 2 cut(s) 458, 833
EcoRII CCWGG 3 cut(s) 138, 454, 681
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 3 cut(s) 73, 661, 878
ErhI CCWWGG 3 cut(s) 73, 661, 878
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FaqI GGGAC 2 cut(s) 97, 795
FauI CCCGC 2 cut(s) 140, 215
Fnu4HI GCNGC 3 cut(s) 204, 888, 1002
FokI GGATG 3 cut(s) 80, 757, 1237
Fsp4HI GCNGC 3 cut(s) 204, 888, 1002
FspBI CTAG 3 cut(s) 557, 846, 1131
GlaI GCGC 1 cut(s) 254
GluI GCNGC 3 cut(s) 204, 888, 1002
GsaI CCCAGC 1 cut(s) 102
GsuI CTGGAG 2 cut(s) 462, 885
HaeIII GGCC 2 cut(s) 143, 460
HapII CCGG 2 cut(s) 373, 1120
HgaI GACGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 255
Hin1I GRCGYC 1 cut(s) 156
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HinfI GANTC 2 cut(s) 356, 574
HpaII CCGG 2 cut(s) 373, 1120
HphI GGTGA 2 cut(s) 281, 1061
Hpy166II GTNNAC 1 cut(s) 757
Hpy188I TCNGA 4 cut(s) 81, 154, 312, 1186
Hpy188III TCNNGA 5 cut(s) 68, 373, 441, 1060, 1198
Hpy8I GTNNAC 1 cut(s) 757
Hpy99I CGWCG 2 cut(s) 158, 161
HpyAV CCTTC 3 cut(s) 193, 274, 471
HpyCH4III ACNGT 4 cut(s) 52, 344, 614, 673
HpyCH4IV ACGT 2 cut(s) 156, 1212
HpyCH4V TGCA 8 cut(s) 48, 57, 414, 608, 628, 859, 887, 1238
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 222, 231, 392, 1007
HpyF3I CTNAG 4 cut(s) 396, 891, 908, 1185
HpySE526I ACGT 2 cut(s) 156, 1212
Hsp92I GRCGYC 1 cut(s) 156
HspAI GCGC 1 cut(s) 253
Kpn2I TCCGGA 1 cut(s) 372
KpnI GGTACC 1 cut(s) 1119
Kzo9I GATC 1 cut(s) 559
LmnI GCTCC 5 cut(s) 266, 866, 998, 1039, 1089
Lsp1109I GCAGC 2 cut(s) 899, 1013
LweI GCATC 5 cut(s) 441, 832, 1063, 1179, 1215
MaeI CTAG 3 cut(s) 557, 846, 1131
MaeII ACGT 2 cut(s) 156, 1212
MalI GATC 1 cut(s) 561
MboI GATC 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 871
MmeI TCCRAC 2 cut(s) 177, 1128
MroI TCCGGA 1 cut(s) 372
MroXI GAANNNNTTC 2 cut(s) 355, 900
MseI TTAA 2 cut(s) 285, 1167
MslI CAYNNNNRTG 1 cut(s) 567
MspA1I CMGCKG 1 cut(s) 890
MspI CCGG 2 cut(s) 373, 1120
MspR9I CCNGG 4 cut(s) 140, 456, 683, 1120
MvaI CCWGG 3 cut(s) 140, 456, 683
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 54, 222, 231, 392, 1007
NciI CCSGG 1 cut(s) 1120
NdeII GATC 1 cut(s) 559
NlaIV GGNNCC 3 cut(s) 834, 1085, 1117
NmeAIII GCCGAG 1 cut(s) 695
NspV TTCGAA 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PdmI GAANNNNTTC 2 cut(s) 355, 900
PfeI GAWTC 2 cut(s) 356, 574
PkrI GCNGC 3 cut(s) 205, 889, 1003
PpuMI RGGWCCY 1 cut(s) 833
PshAI GACNNNNGTC 1 cut(s) 75
PshBI ATTAAT 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 833
Psp6I CCWGG 3 cut(s) 138, 454, 681
PspFI CCCAGC 1 cut(s) 98
PspGI CCWGG 3 cut(s) 138, 454, 681
PspN4I GGNNCC 3 cut(s) 834, 1085, 1117
PspPI GGNCC 4 cut(s) 77, 446, 458, 833
PspPPI RGGWCCY 1 cut(s) 833
PsrI GAACNNNNNNTAC 4 cut(s) 670, 702, 740, 772
PvuII CAGCTG 1 cut(s) 890
RsaI GTAC 4 cut(s) 92, 594, 758, 1117
RsaNI GTAC 4 cut(s) 91, 593, 757, 1116
RseI CAYNNNNRTG 1 cut(s) 567
SaqAI TTAA 2 cut(s) 285, 1167
SatI GCNGC 3 cut(s) 204, 888, 1002
Sau3AI GATC 1 cut(s) 559
Sau96I GGNCC 4 cut(s) 77, 446, 458, 833
ScrFI CCNGG 4 cut(s) 140, 456, 683, 1120
SduI GDGCHC 1 cut(s) 871
SfaNI GCATC 5 cut(s) 441, 832, 1063, 1179, 1215
SfcI CTRYAG 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 3 cut(s) 77, 446, 833
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 1 cut(s) 795
SmoI CTYRAG 1 cut(s) 795
SsiI CCGC 5 cut(s) 133, 190, 204, 208, 214
SspI AATATT 1 cut(s) 44
SspMI CTAG 3 cut(s) 557, 846, 1131
StyD4I CCNGG 4 cut(s) 138, 454, 681, 1118
StyI CCWWGG 3 cut(s) 73, 661, 878
TaaI ACNGT 4 cut(s) 52, 344, 614, 673
TaiI ACGT 2 cut(s) 159, 1215
TaqI TCGA 3 cut(s) 39, 195, 240
TaqII GACCGA 1 cut(s) 463
TatI WGTACW 1 cut(s) 90
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 2 cut(s) 356, 574
Tru1I TTAA 2 cut(s) 285, 1167
Tru9I TTAA 2 cut(s) 285, 1167
TseI GCWGC 2 cut(s) 887, 1001
TspDTI ATGAA 4 cut(s) 17, 753, 761, 794
TspGWI ACGGA 1 cut(s) 780
VpaK11BI GGWCC 3 cut(s) 77, 446, 833
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 8 cut(s) 316, 368, 495, 530, 655, 819, 900, 1139
XmnI GAANNNNTTC 2 cut(s) 355, 900
XspI CTAG 3 cut(s) 557, 846, 1131
ZraI GACGTC 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.