pycom06g13140

TITAN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0007112
Physical Location & Seq
Reverse (-)
18189846 .. 18191602
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13140.1

Sequence Viewer

Length: 897 bp
ATGTTCCAGTTGTATGCAGGAGAAGAGGAAAGATCGATCACGAAGAAGAAGAAGAAGTACGAGTTCGAGTACTGCGTGGTTTGCAAGCTCAATCACGACCAAGGTCCGAGCCACAAGTACATCCCGAACCACACCAAGTCCCTCTCCGCCTTCCTCTCTCGCTTCCAGTCCAAGCTCGCCGACGTCCGCTTCTTTCTCAAAAACCCTAGCCCTCTCCGCCCCGAACACGCCTCCCGCAATCGCCTCTGGTGCGTCTTCTGCGACTCCGATATCGACGAGCTCTATAGCTCCTTCGCCTGTGGTAATGCGATTAATCACCTTGCGACCGCTGAACATCTGAAGAATTTGAAGCATTTCTTGTGGAAATACGGTGGAGGAATGGACCGCATTGATACATTTAGAATTTCGGAAAATGAACTTGCTAAGTGGGAGAAGAAGTGTAATTCACTGAAGCTTGAAGCTGTGCCTTGTGGTGAAGGCTCTCGAGGACCGGCATTTGGACCTTCGAATGATATCCATACTGAACTGAAATATGGAATTATAGATACTTCTGAAAATAATAGTAATTCATGTTTTTCCCATGGTGTCATTCCTTTACCATATCATACAAATGAGAATCAGGTATCCCATTCAGGACTCCCTCAAGATACAAATGTTGGCTGCTTTCCGCAAGCTGTTGCTAACGTTTCTTATCATGTGGGAATACGGTCTGGTACAAATTCCATAAATTCAAACGGTTTAACAGGCTTATGCGTGTTTTGTGCCCTCTGGTGCTTACTCACTCACCCAGTTAGCAGGAGTACCCAACATCCTTTGGTTTCTAATGGTAGAAAATTCTCAGCTGATGGTTACTTCGATAACGAAAGGGTAAGTTTGATCATCCACACTCTTCACTAA

Protein Analysis

299

Amino Acids

33.51

Weight (kDa)

8.21

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC84 PF14968 24 - 213 2.7e-52 Coiled coil protein 84
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
AciI CCGC 7 cut(s) 147, 187, 217, 235, 327, 385, 668
AclI AACGTT 1 cut(s) 684
AcsI RAATTY 5 cut(s) 343, 402, 718, 727, 833
AcuI CTGAAG 2 cut(s) 359, 470
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 5 cut(s) 59, 71, 119, 715, 802
AfiI CCNNNNNNNGG 1 cut(s) 497
AgsI TTSAA 3 cut(s) 349, 458, 732
AluBI AGCT 8 cut(s) 88, 175, 280, 288, 454, 461, 674, 842
AluI AGCT 8 cut(s) 88, 175, 280, 288, 454, 461, 674, 842
Alw21I GWGCWC 1 cut(s) 282
Ama87I CYCGRG 1 cut(s) 483
ApeKI GCWGC 1 cut(s) 660
ApoI RAATTY 5 cut(s) 343, 402, 718, 727, 833
AseI ATTAAT 1 cut(s) 312
Asp700I GAANNNNTTC 1 cut(s) 353
AspS9I GGNCC 4 cut(s) 104, 382, 488, 500
AsuHPI GGTGA 3 cut(s) 308, 485, 776
AsuII TTCGAA 1 cut(s) 506
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 4 cut(s) 104, 382, 488, 500
BaeGI GKGCMC 1 cut(s) 766
BaeI ACNNNNGTAYC 2 cut(s) 639, 672
BanII GRGCYC 1 cut(s) 282
BarI GAAGNNNNNNTAC 2 cut(s) 41, 73
BbsI GAAGAC 1 cut(s) 247
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 647
BccI CCATC 1 cut(s) 839
BciVI GTATCC 1 cut(s) 634
BclI TGATCA 1 cut(s) 876
BfaI CTAG 1 cut(s) 207
BfmI CTRYAG 1 cut(s) 283
BfuI GTATCC 1 cut(s) 634
BisI GCNGC 1 cut(s) 661
BlsI GCNGC 1 cut(s) 662
BmcAI AGTACT 1 cut(s) 71
Bme18I GGWCC 4 cut(s) 104, 382, 488, 500
BmeT110I CYCGRG 1 cut(s) 483
BmgT120I GGNCC 4 cut(s) 104, 382, 488, 500
BmrI ACTGGG 1 cut(s) 782
BmuI ACTGGG 1 cut(s) 782
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 247
Bpu14I TTCGAA 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 627
Bsa29I ATCGAT 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 100, 580
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 497
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 3 cut(s) 7, 166, 788
BseCI ATCGAT 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 100, 580
BseGI GGATG 3 cut(s) 120, 808, 879
BseLI CCNNNNNNNGG 1 cut(s) 497
BseMII CTCAG 1 cut(s) 852
BseNI ACTGG 3 cut(s) 7, 166, 788
BseSI GKGCMC 1 cut(s) 766
BseXI GCAGC 1 cut(s) 647
Bsh1285I CGRYCG 1 cut(s) 327
BshVI ATCGAT 1 cut(s) 35
BsiEI CGRYCG 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 1 cut(s) 491
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 124
BsoBI CYCGRG 1 cut(s) 483
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 282, 766
Bsp143I GATC 3 cut(s) 32, 36, 876
Bsp19I CCATGG 1 cut(s) 580
BspACI CCGC 7 cut(s) 147, 187, 217, 235, 327, 385, 668
BspCNI CTCAG 1 cut(s) 851
BspDI ATCGAT 1 cut(s) 35
BspT104I TTCGAA 1 cut(s) 506
BsrFI RCCGGY 1 cut(s) 490
BsrI ACTGG 3 cut(s) 7, 166, 788
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 100, 580
BssMI GATC 3 cut(s) 32, 36, 876
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 2 cut(s) 100, 580
Bst4CI ACNGT 3 cut(s) 371, 708, 737
Bst6I CTCTTC 2 cut(s) 18, 894
BstACI GRCGYC 1 cut(s) 183
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 3 cut(s) 86, 177, 672
BstDEI CTNAG 2 cut(s) 423, 838
BstDSI CCRYGG 1 cut(s) 580
BstF5I GGATG 3 cut(s) 120, 808, 879
BstKTI GATC 3 cut(s) 35, 39, 879
BstMBI GATC 3 cut(s) 32, 36, 876
BstMCI CGRYCG 1 cut(s) 327
BstMWI GCNNNNNNNGC 4 cut(s) 81, 216, 249, 258
BstPAI GACNNNNGTC 1 cut(s) 102
BstSFI CTRYAG 1 cut(s) 283
BstSLI GKGCMC 1 cut(s) 766
BstV1I GCAGC 1 cut(s) 647
BstV2I GAAGAC 1 cut(s) 247
Bsu15I ATCGAT 1 cut(s) 35
BsuI GTATCC 1 cut(s) 634
BsuTUI ATCGAT 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 580
BtsCI GGATG 3 cut(s) 120, 808, 879
BtsIMutI CAGTG 1 cut(s) 446
Cac8I GCNNGC 3 cut(s) 86, 177, 672
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 4 cut(s) 104, 382, 488, 500
ClaI ATCGAT 1 cut(s) 35
CseI GACGC 1 cut(s) 241
Csp6I GTAC 5 cut(s) 58, 70, 118, 714, 801
CviAII CATG 3 cut(s) 570, 581, 695
CviQI GTAC 5 cut(s) 58, 70, 118, 714, 801
DdeI CTNAG 2 cut(s) 423, 838
DpnI GATC 3 cut(s) 34, 38, 878
DpnII GATC 3 cut(s) 32, 36, 876
Eam1104I CTCTTC 2 cut(s) 18, 894
EarI CTCTTC 2 cut(s) 18, 894
EciI GGCGGA 2 cut(s) 136, 206
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 100, 580
Eco24I GRGCYC 1 cut(s) 282
Eco32I GATATC 2 cut(s) 271, 514
Eco47I GGWCC 4 cut(s) 104, 382, 488, 500
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 2 cut(s) 359, 470
Eco88I CYCGRG 1 cut(s) 483
EcoICRI GAGCTC 1 cut(s) 280
EcoRV GATATC 2 cut(s) 271, 514
EcoT14I CCWWGG 2 cut(s) 100, 580
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 100, 580
FaeI CATG 3 cut(s) 573, 584, 698
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 1 cut(s) 124
FatI CATG 3 cut(s) 569, 580, 694
FauI CCCGC 1 cut(s) 242
FbaI TGATCA 1 cut(s) 876
Fnu4HI GCNGC 1 cut(s) 661
FokI GGATG 3 cut(s) 107, 795, 866
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 1 cut(s) 661
FspBI CTAG 1 cut(s) 207
GluI GCNGC 1 cut(s) 661
HapII CCGG 1 cut(s) 491
HgaI GACGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 573, 584, 698
HindIII AAGCTT 1 cut(s) 452
HinfI GANTC 3 cut(s) 263, 616, 636
HpaII CCGG 1 cut(s) 491
HphI GGTGA 3 cut(s) 308, 485, 776
Hpy188I TCNGA 5 cut(s) 108, 268, 339, 409, 553
Hpy188III TCNNGA 6 cut(s) 40, 95, 124, 483, 633, 644
Hpy99I CGWCG 2 cut(s) 185, 278
HpyAV CCTTC 4 cut(s) 160, 301, 470, 513
HpyCH4III ACNGT 3 cut(s) 371, 708, 737
HpyCH4IV ACGT 2 cut(s) 183, 684
HpyCH4V TGCA 2 cut(s) 17, 84
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 216, 249, 258
HpyF3I CTNAG 2 cut(s) 423, 838
HpySE526I ACGT 2 cut(s) 183, 684
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 573, 584, 698
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 3 cut(s) 32, 36, 876
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 1 cut(s) 647
MaeI CTAG 1 cut(s) 207
MaeII ACGT 2 cut(s) 183, 684
MaeIII GTNAC 1 cut(s) 848
MalI GATC 3 cut(s) 34, 38, 878
MboI GATC 3 cut(s) 32, 36, 876
MboII GAAGA 9 cut(s) 35, 55, 58, 61, 64, 247, 352, 445, 881
MhlI GDGCHC 2 cut(s) 282, 766
MluCI AATT 8 cut(s) 343, 402, 442, 537, 565, 718, 727, 833
MlyI GAGTC 2 cut(s) 257, 630
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 2 cut(s) 312, 740
MslI CAYNNNNRTG 1 cut(s) 609
MspA1I CMGCKG 2 cut(s) 329, 842
MspI CCGG 1 cut(s) 491
MwoI GCNNNNNNNGC 4 cut(s) 81, 216, 249, 258
NcoI CCATGG 1 cut(s) 580
NdeII GATC 3 cut(s) 32, 36, 876
NlaIII CATG 3 cut(s) 573, 584, 698
NspV TTCGAA 1 cut(s) 506
PaeR7I CTCGAG 1 cut(s) 483
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 616
PkrI GCNGC 1 cut(s) 662
PleI GAGTC 2 cut(s) 257, 630
PpsI GAGTC 2 cut(s) 257, 630
PshAI GACNNNNGTC 1 cut(s) 102
PshBI ATTAAT 1 cut(s) 312
Psp124BI GAGCTC 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 684
PspPI GGNCC 4 cut(s) 104, 382, 488, 500
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 5 cut(s) 59, 71, 119, 715, 802
RsaNI GTAC 5 cut(s) 58, 70, 118, 714, 801
RseI CAYNNNNRTG 1 cut(s) 609
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 2 cut(s) 312, 740
SatI GCNGC 1 cut(s) 661
Sau3AI GATC 3 cut(s) 32, 36, 876
Sau96I GGNCC 4 cut(s) 104, 382, 488, 500
ScaI AGTACT 1 cut(s) 71
SchI GAGTC 2 cut(s) 257, 630
SduI GDGCHC 2 cut(s) 282, 766
SfcI CTRYAG 1 cut(s) 283
Sfr274I CTCGAG 1 cut(s) 483
SfuI TTCGAA 1 cut(s) 506
SinI GGWCC 4 cut(s) 104, 382, 488, 500
SlaI CTCGAG 1 cut(s) 483
SmiMI CAYNNNNRTG 1 cut(s) 609
SmlI CTYRAG 2 cut(s) 483, 642
SmoI CTYRAG 2 cut(s) 483, 642
Sse9I AATT 8 cut(s) 343, 402, 442, 537, 565, 718, 727, 833
SsiI CCGC 7 cut(s) 147, 187, 217, 235, 327, 385, 668
SspMI CTAG 1 cut(s) 207
SstI GAGCTC 1 cut(s) 282
StyI CCWWGG 2 cut(s) 100, 580
TaaI ACNGT 3 cut(s) 371, 708, 737
TaiI ACGT 2 cut(s) 186, 687
TaqI TCGA 6 cut(s) 35, 66, 273, 484, 506, 855
TasI AATT 8 cut(s) 343, 402, 442, 537, 565, 718, 727, 833
TatI WGTACW 2 cut(s) 69, 117
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 2 cut(s) 312, 740
Tru9I TTAA 2 cut(s) 312, 740
TscAI CASTG 1 cut(s) 453
TseI GCWGC 1 cut(s) 660
TspDTI ATGAA 2 cut(s) 429, 558
TspRI CASTG 1 cut(s) 453
VpaK11BI GGWCC 4 cut(s) 104, 382, 488, 500
VspI ATTAAT 1 cut(s) 312
XapI RAATTY 5 cut(s) 343, 402, 718, 727, 833
XhoI CTCGAG 1 cut(s) 483
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 1 cut(s) 207
ZraI GACGTC 1 cut(s) 184
ZrmI AGTACT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.