AT4G27460

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003827
Physical Location & Seq
Forward (+)
13732832 .. 13734531
1700 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27460.1

Sequence Viewer

Length: 1176 bp
ATGGCACTGTCCCTTCTCTCCTACAACGTCTCCGACCTCTGTCTCGGCAAACCTCCTCTTCGTTGTCTTTCCTCCTCTTCCTCCTCCGTATCCGACGCCATCGCTGCCCTCAAATCCTCTGAAGACACTTTCCTCTCCGTCTGGAATTGCAATCACGACGATGACAATAACACAGAGTGCGAGTGCTTGGGGAAAATATCAATGGCTGACGTCATCTGCCACTTGTCCAAAGACCATGACCACAGTCTCTGTGCCTTAAACTCATCCGTCTCTGTTCTTCTCCCCAAAACTCGTTCCATTGTCCTCCATGTTCAACCATCCTGCAGCTTAATAGAAGCCATTGATCTGATAATCAAAGGAGCACAGAATCTGATTGTCCCGATACATACAAAGCCCTACACTAAGAAAAAGCAGCACAACGACAACGTTTCGGTCACCACCACCACCCACTCGAACGGACAACGATTCTGCTGGATCACACAAGAAGACATCATTCAGTTTCTCCTCGGATTCATCGCCGCATTCTCTCCTTTGCCAGCGATGTCCCTCTCTGATCTCGGTGTCATCAACAGCACACACACAGTTGTTGCGGTCGATTACCACTCCTCAGCGTCCGCAGTTGTCTCTGCCGTATCCAACGCTCTTGCCGTCCAAACCTCTGTCGCAGTGGTCGACGGTGAGGGAGATGACCCTTTTACGTCTTTGATCGGCGAAATATCCCCGATGACATTAACTTGCTGCGACGAGACAGCAGCTGCGGCGGTGGCGACGCTCTCCGCTGGAGATCTGATGGCGTACATAGACGGAGCAAATCCTCCGGAGAGTCTTGTTCAGATCGTTAGGAACCGTTTGGAGGATAAAGGATTGATTGGTTTGATGTCGCTTTTCGATTCTCTCTCATCTTATTCGACGTCGTCGGGTTATTCGTCGGAGGAAGAAGCTCCAGTGAGAACGACGTCGTATGGACGGTCGATGAGTAGCTCAGCAAGAATGGCAAGGAAGTCGGAGGCGATAGTGTGTAATCCAAAGAGCTCGTTAATGGCAGTGATGATCCAAGCGGTTGCTCATCGAGTGAACTACGCGTGGGTGGTTGAGAAGGATGGTTGTTTCGTTGGCATGGTTACTTTTGTTGATATCTTAAAAGTTTTTAGGAAATTTTTGGAGAATGACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

391

Amino Acids

41.96

Weight (kDa)

5.16

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 213, 914, 959
AccI GTMKAC 1 cut(s) 672
AccII CGCG 1 cut(s) 1082
AccIII TCCGGA 1 cut(s) 817
AciI CCGC 7 cut(s) 519, 590, 615, 758, 761, 777, 1058
AclI AACGTT 1 cut(s) 426
AclWI GGATC 2 cut(s) 482, 1045
AcsI RAATTY 1 cut(s) 1154
AcuI CTGAAG 1 cut(s) 141
AcyI GRCGYC 4 cut(s) 96, 210, 911, 956
AdeI CACNNNGTG 1 cut(s) 177
AfaI GTAC 1 cut(s) 797
AfiI CCNNNNNNNGG 1 cut(s) 853
AflIII ACRYGT 2 cut(s) 1080, 1170
AgsI TTSAA 1 cut(s) 314
AluBI AGCT 5 cut(s) 327, 755, 941, 981, 1032
AluI AGCT 5 cut(s) 327, 755, 941, 981, 1032
Alw21I GWGCWC 2 cut(s) 364, 1034
Alw26I GTCTC 6 cut(s) 34, 47, 251, 274, 628, 740
AlwI GGATC 2 cut(s) 482, 1045
AlwNI CAGNNNCTG 3 cut(s) 249, 370, 755
Aor13HI TCCGGA 1 cut(s) 817
ApeKI GCWGC 6 cut(s) 104, 324, 412, 738, 752, 755
ApoI RAATTY 1 cut(s) 1154
AsuHPI GGTGA 2 cut(s) 427, 689
BanII GRGCYC 1 cut(s) 1034
BbsI GAAGAC 2 cut(s) 129, 492
Bbv12I GWGCWC 2 cut(s) 364, 1034
BbvCI CCTCAGC 1 cut(s) 607
BbvI GCAGC 6 cut(s) 91, 336, 424, 725, 742, 764
BccI CCATC 4 cut(s) 107, 325, 784, 1094
BceAI ACGGC 2 cut(s) 614, 632
BcgI CGANNNNNNTGC 2 cut(s) 984, 1018
BciVI GTATCC 2 cut(s) 100, 643
BcoDI GTCTC 6 cut(s) 34, 47, 251, 274, 628, 740
BfmI CTRYAG 1 cut(s) 322
BfuI GTATCC 2 cut(s) 100, 643
BglII AGATCT 1 cut(s) 784
BisI GCNGC 8 cut(s) 105, 325, 413, 519, 739, 753, 756, 759
BlpI GCTNAGC 1 cut(s) 982
BlsI GCNGC 8 cut(s) 106, 326, 414, 520, 740, 754, 757, 760
BmiI GGNNCC 1 cut(s) 845
BoxI GACNNNNGTC 2 cut(s) 39, 243
BpiI GAAGAC 2 cut(s) 129, 492
BpmI CTGGAG 2 cut(s) 801, 927
Bpu10I CCTNAGC 1 cut(s) 607
Bpu1102I GCTNAGC 1 cut(s) 982
BsaHI GRCGYC 4 cut(s) 96, 210, 911, 956
BsaJI CCNNGG 1 cut(s) 505
BsaWI WCCGGW 1 cut(s) 817
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 1 cut(s) 853
Bse1I ACTGG 1 cut(s) 944
BseAI TCCGGA 1 cut(s) 817
BseDI CCNNGG 1 cut(s) 505
BseGI GGATG 3 cut(s) 263, 317, 1105
BseLI CCNNNNNNNGG 1 cut(s) 853
BseMII CTCAG 2 cut(s) 621, 996
BseNI ACTGG 1 cut(s) 944
BseRI GAGGAG 5 cut(s) 45, 64, 73, 494, 595
BseXI GCAGC 6 cut(s) 91, 336, 424, 725, 742, 764
Bsh1236I CGCG 1 cut(s) 1082
Bsh1285I CGRYCG 2 cut(s) 594, 971
BsiEI CGRYCG 2 cut(s) 594, 971
BsiHKAI GWGCWC 2 cut(s) 364, 1034
BsiSI CCGG 1 cut(s) 818
BslFI GGGAC 2 cut(s) 362, 529
BslI CCNNNNNNNGG 1 cut(s) 853
BsmAI GTCTC 6 cut(s) 34, 47, 251, 274, 628, 740
BsmBI CGTCTC 2 cut(s) 34, 274
BsmFI GGGAC 2 cut(s) 362, 529
BsmI GAATGC 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 364, 1034
Bsp13I TCCGGA 1 cut(s) 817
Bsp143I GATC 7 cut(s) 343, 474, 553, 705, 784, 834, 1050
Bsp1720I GCTNAGC 1 cut(s) 982
BspACI CCGC 7 cut(s) 519, 590, 615, 758, 761, 777, 1058
BspCNI CTCAG 2 cut(s) 620, 995
BspEI TCCGGA 1 cut(s) 817
BspFNI CGCG 1 cut(s) 1082
BspLI GGNNCC 1 cut(s) 845
BspMAI CTGCAG 1 cut(s) 326
BspPI GGATC 2 cut(s) 482, 1045
BsrI ACTGG 1 cut(s) 944
BssECI CCNNGG 1 cut(s) 505
BssMI GATC 7 cut(s) 343, 474, 553, 705, 784, 834, 1050
BssNI GRCGYC 4 cut(s) 96, 210, 911, 956
Bst4CI ACNGT 6 cut(s) 9, 245, 583, 677, 848, 969
Bst6I CTCTTC 2 cut(s) 63, 82
BstACI GRCGYC 4 cut(s) 96, 210, 911, 956
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 3 cut(s) 402, 607, 982
BstEII GGTNACC 1 cut(s) 433
BstF5I GGATG 3 cut(s) 263, 317, 1105
BstFNI CGCG 1 cut(s) 1082
BstKTI GATC 7 cut(s) 346, 477, 556, 708, 787, 837, 1053
BstMAI GTCTC 6 cut(s) 34, 47, 251, 274, 628, 740
BstMBI GATC 7 cut(s) 343, 474, 553, 705, 784, 834, 1050
BstMCI CGRYCG 2 cut(s) 594, 971
BstMWI GCNNNNNNNGC 4 cut(s) 104, 758, 764, 992
BstNSI RCATGY 1 cut(s) 1174
BstPAI GACNNNNGTC 2 cut(s) 39, 243
BstPI GGTNACC 1 cut(s) 433
BstSFI CTRYAG 1 cut(s) 322
BstUI CGCG 1 cut(s) 1082
BstV1I GCAGC 6 cut(s) 91, 336, 424, 725, 742, 764
BstV2I GAAGAC 2 cut(s) 129, 492
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BsuI GTATCC 2 cut(s) 100, 643
BtgZI GCGATG 3 cut(s) 85, 499, 554
BtsCI GGATG 3 cut(s) 263, 317, 1105
BtsI GCAGTG 2 cut(s) 672, 1050
BtsIMutI CAGTG 4 cut(s) 5, 672, 951, 1050
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 3 cut(s) 249, 370, 755
CseI GACGC 3 cut(s) 104, 600, 778
Csp6I GTAC 1 cut(s) 796
CviAII CATG 4 cut(s) 236, 308, 1117, 1171
CviJI RGCY 8 cut(s) 206, 327, 338, 394, 755, 941, 981, 1032
CviKI_1 RGCY 8 cut(s) 206, 327, 338, 394, 755, 941, 981, 1032
CviQI GTAC 1 cut(s) 796
DdeI CTNAG 3 cut(s) 402, 607, 982
DpnI GATC 7 cut(s) 345, 476, 555, 707, 786, 836, 1052
DpnII GATC 7 cut(s) 343, 474, 553, 705, 784, 834, 1050
DraIII CACNNNGTG 1 cut(s) 177
Eam1104I CTCTTC 2 cut(s) 63, 82
EarI CTCTTC 2 cut(s) 63, 82
Ecl136II GAGCTC 1 cut(s) 1032
Eco24I GRGCYC 1 cut(s) 1034
Eco32I GATATC 1 cut(s) 1135
Eco53kI GAGCTC 1 cut(s) 1032
Eco57I CTGAAG 1 cut(s) 141
Eco91I GGTNACC 1 cut(s) 433
EcoICRI GAGCTC 1 cut(s) 1032
EcoO65I GGTNACC 1 cut(s) 433
EcoRV GATATC 1 cut(s) 1135
EcoT38I GRGCYC 1 cut(s) 1034
Esp3I CGTCTC 2 cut(s) 34, 274
FaeI CATG 4 cut(s) 239, 311, 1120, 1174
FaiI YATR 7 cut(s) 237, 309, 387, 800, 963, 1118, 1172
FaqI GGGAC 2 cut(s) 362, 529
FatI CATG 4 cut(s) 235, 307, 1116, 1170
FblI GTMKAC 1 cut(s) 672
Fnu4HI GCNGC 8 cut(s) 105, 325, 413, 519, 739, 753, 756, 759
FokI GGATG 3 cut(s) 250, 304, 1112
FriOI GRGCYC 1 cut(s) 1034
Fsp4HI GCNGC 8 cut(s) 105, 325, 413, 519, 739, 753, 756, 759
GluI GCNGC 8 cut(s) 105, 325, 413, 519, 739, 753, 756, 759
GsuI CTGGAG 2 cut(s) 801, 927
HapII CCGG 1 cut(s) 818
HgaI GACGC 3 cut(s) 104, 600, 778
Hin1I GRCGYC 4 cut(s) 96, 210, 911, 956
Hin1II CATG 4 cut(s) 239, 311, 1120, 1174
HincII GTYRAC 1 cut(s) 673
HindII GTYRAC 1 cut(s) 673
HinfI GANTC 5 cut(s) 367, 465, 510, 823, 890
HpaII CCGG 1 cut(s) 818
HphI GGTGA 2 cut(s) 427, 689
Hpy166II GTNNAC 2 cut(s) 673, 1075
Hpy188III TCNNGA 4 cut(s) 142, 155, 379, 818
Hpy8I GTNNAC 2 cut(s) 673, 1075
HpyAV CCTTC 2 cut(s) 23, 1090
HpyCH4III ACNGT 6 cut(s) 9, 245, 583, 677, 848, 969
HpyCH4IV ACGT 6 cut(s) 27, 210, 426, 698, 911, 956
HpyCH4V TGCA 2 cut(s) 150, 324
HpyF10VI GCNNNNNNNGC 4 cut(s) 104, 758, 764, 992
HpyF3I CTNAG 3 cut(s) 402, 607, 982
HpySE526I ACGT 6 cut(s) 27, 210, 426, 698, 911, 956
Hsp92I GRCGYC 4 cut(s) 96, 210, 911, 956
Hsp92II CATG 4 cut(s) 239, 311, 1120, 1174
Kpn2I TCCGGA 1 cut(s) 817
Kzo9I GATC 7 cut(s) 343, 474, 553, 705, 784, 834, 1050
LmnI GCTCC 3 cut(s) 359, 806, 946
LpnPI CCDG 7 cut(s) 127, 334, 457, 549, 765, 831, 957
Lsp1109I GCAGC 6 cut(s) 91, 336, 424, 725, 742, 764
MaeII ACGT 6 cut(s) 27, 210, 426, 698, 911, 956
MaeIII GTNAC 2 cut(s) 433, 1120
MalI GATC 7 cut(s) 345, 476, 555, 707, 786, 836, 1052
MboI GATC 7 cut(s) 343, 474, 553, 705, 784, 834, 1050
MboII GAAGA 6 cut(s) 50, 69, 134, 269, 497, 947
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 364, 1034
MluCI AATT 2 cut(s) 145, 1154
MluI ACGCGT 1 cut(s) 1080
MlyI GAGTC 1 cut(s) 832
MmeI TCCRAC 5 cut(s) 57, 117, 660, 909, 984
MroI TCCGGA 1 cut(s) 817
MseI TTAA 5 cut(s) 257, 329, 731, 1037, 1139
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 2 cut(s) 755, 779
MspI CCGG 1 cut(s) 818
Mva1269I GAATGC 1 cut(s) 521
MvnI CGCG 1 cut(s) 1082
MwoI GCNNNNNNNGC 4 cut(s) 104, 758, 764, 992
NdeII GATC 7 cut(s) 343, 474, 553, 705, 784, 834, 1050
NlaIII CATG 4 cut(s) 239, 311, 1120, 1174
NlaIV GGNNCC 1 cut(s) 845
NmeAIII GCCGAG 1 cut(s) 24
NmuCI GTSAC 1 cut(s) 433
NspI RCATGY 1 cut(s) 1174
PciI ACATGT 1 cut(s) 1170
PcsI WCGNNNNNNNCGW 3 cut(s) 645, 669, 923
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 4 cut(s) 367, 465, 510, 890
PflFI GACNNNGTC 1 cut(s) 913
PkrI GCNGC 8 cut(s) 106, 326, 414, 520, 740, 754, 757, 760
PleI GAGTC 1 cut(s) 831
PpsI GAGTC 1 cut(s) 831
PscI ACATGT 1 cut(s) 1170
PshAI GACNNNNGTC 2 cut(s) 39, 243
Psp124BI GAGCTC 1 cut(s) 1034
Psp1406I AACGTT 1 cut(s) 426
PspEI GGTNACC 1 cut(s) 433
PspN4I GGNNCC 1 cut(s) 845
PstI CTGCAG 1 cut(s) 326
PstNI CAGNNNCTG 3 cut(s) 249, 370, 755
PsuI RGATCY 1 cut(s) 784
PsyI GACNNNGTC 1 cut(s) 913
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 1 cut(s) 797
RsaNI GTAC 1 cut(s) 796
RseI CAYNNNNRTG 1 cut(s) 159
SacI GAGCTC 1 cut(s) 1034
SalI GTCGAC 1 cut(s) 671
SaqAI TTAA 5 cut(s) 257, 329, 731, 1037, 1139
SatI GCNGC 8 cut(s) 105, 325, 413, 519, 739, 753, 756, 759
Sau3AI GATC 7 cut(s) 343, 474, 553, 705, 784, 834, 1050
SchI GAGTC 1 cut(s) 832
SduI GDGCHC 2 cut(s) 364, 1034
SfcI CTRYAG 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 159
Sse9I AATT 2 cut(s) 145, 1154
SsiI CCGC 7 cut(s) 519, 590, 615, 758, 761, 777, 1058
SstI GAGCTC 1 cut(s) 1034
TaaI ACNGT 6 cut(s) 9, 245, 583, 677, 848, 969
TaiI ACGT 6 cut(s) 30, 213, 429, 701, 914, 959
TaqI TCGA 7 cut(s) 452, 594, 672, 888, 908, 971, 1069
TaqII GACCGA 1 cut(s) 421
TasI AATT 2 cut(s) 145, 1154
TauI GCSGC 2 cut(s) 521, 761
TfiI GAWTC 4 cut(s) 367, 465, 510, 890
Tru1I TTAA 5 cut(s) 257, 329, 731, 1037, 1139
Tru9I TTAA 5 cut(s) 257, 329, 731, 1037, 1139
TscAI CASTG 4 cut(s) 12, 672, 951, 1050
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 6 cut(s) 104, 324, 412, 738, 752, 755
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 1 cut(s) 502
TspGWI ACGGA 5 cut(s) 76, 127, 256, 471, 819
TspRI CASTG 4 cut(s) 12, 672, 951, 1050
Tth111I GACNNNGTC 1 cut(s) 913
XapI RAATTY 1 cut(s) 1154
XceI RCATGY 1 cut(s) 1174
XmiI GTMKAC 1 cut(s) 672
ZraI GACGTC 3 cut(s) 211, 912, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.