FvH4_3g20550

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003827
Physical Location & Seq
Reverse (-)
13605521 .. 13607466
1946 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20550.t1

Sequence Viewer

Length: 1218 bp
ATGGCAGTGAGCTTTTTAAGGCACGAGGTATCTGACCTCTGTCTGGGCAAGCCGGCTTTGAGGTCTCTCTCCATCTCCGCCACCGTCGCTGATGCCGTTGAAGCCTTGAGGACCTCGGAAGATAACTTCATCAGCGTCTGGGATTGCAACCACCACTCCAAGAGTCTCGCCGGCGACCAGTGCCGCTGTGTTGGGAAGGTTTGCATGGTCGACGTCATTTGCTATCTTTCTAAGGATGAGAACCTGTCGTCTCCATCTGCTGCGCTGAAGGCACCCGTCTCTGAAATCTTGACAAAGATTCCGGGAACTGTTATGCACGTCGAGCCATCTTGCAGCTTATTGCAGGCCATTGATCTAATTCTCCAAGGAAACCAGAACCTGGTAGTACCAATTCAGACCAGGCAAAGCAGCACTTTAAGAAGAAAACAGTTCCCAAAATCTACAAACCAAATGGCCACAACAACCAATCACAACGGTCAAGAATTCTGCTGGCTAACACAGGAAGACGTAATCCGATTCCTTCTGAGCTCGATCGGGGTCTTCTCTCCCATGCCTGCTCTCTCAATCGACTCTCTCGGCATCATCAACACTATAGACATCTTGGCCATCAACTACCACTCCCCGGCAAGCTCCGCCGTCCAGCTGATAACTCAGTCACTCGAGCAGCAAACCTCAGTGGCTGTTGTGGACAGTGATGATGTGTTGATCGGTGAGATCTCGCCTTTCACTCTGGCCTGCTGTGACGAGTCGGTGGCTGCAGCCATCGCCACTCTGTCTTGCGGAGAGCTCATGTCCTACATTGACTGTGGAGGACCACCAGAGCACTTGATCAAGATTGTCACGGAGAGATTGAAGGAGAGGAAGTTAAAAAGAGCTTTGGAAAATTACTCATCAGTAATTAATGCCTCTGCAAACTCAATGTCGTCGTCTTCTTCTTCTTCTTCATCAGATGAGGAGTCCTCTGTATCTTCAAGTAGTAGCAAACTCAGGTCTGCAGGAAGATTTAGTAGGTCAAATAGCTACTCGGCTAGAATGGTGAGAAGGTCTGAGGCCATAGTTTGTCATCCAAAGAGTTCGTTGGTGGCTGTGATGATTCAGGCTATTGCTCATAGAGTGAACTATGTCTGGGTTATTGAGGATGACTATAACTTGGTGGGTGTTGTCACTTTTACCGGCATGTTAGAGGTTTTCCGGGAACACTTGGAGACCATGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

406

Amino Acids

44.0

Weight (kDa)

5.61

Isoelectric Point (pI)

59.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 271
AccB7I CCANNNNNTGG 1 cut(s) 379
AccI GTMKAC 1 cut(s) 210
AciI CCGC 4 cut(s) 78, 184, 633, 780
AcoI YGGCCR 2 cut(s) 453, 603
AcsI RAATTY 1 cut(s) 482
AcuI CTGAAG 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 3 cut(s) 43, 379, 622
AgsI TTSAA 3 cut(s) 101, 853, 972
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 2 cut(s) 378, 398
AluBI AGCT 8 cut(s) 12, 336, 528, 630, 643, 787, 875, 1020
AluI AGCT 8 cut(s) 12, 336, 528, 630, 643, 787, 875, 1020
Alw21I GWGCWC 3 cut(s) 530, 789, 825
Alw26I GTCTC 5 cut(s) 69, 170, 255, 283, 1199
AlwNI CAGNNNCTG 3 cut(s) 138, 379, 680
Ama87I CYCGRG 1 cut(s) 659
AoxI GGCC 5 cut(s) 345, 453, 603, 732, 1050
ApeKI GCWGC 6 cut(s) 260, 333, 408, 664, 755, 758
ApoI RAATTY 1 cut(s) 482
ArsI GACNNNNNNTTYG 2 cut(s) 905, 937
AseI ATTAAT 1 cut(s) 900
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 2 cut(s) 111, 812
AsuC2I CCSGG 3 cut(s) 303, 623, 1193
AsuHPI GGTGA 2 cut(s) 722, 1048
AvaI CYCGRG 1 cut(s) 659
AvaII GGWCC 2 cut(s) 111, 812
BalI TGGCCA 2 cut(s) 455, 605
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 2 cut(s) 530, 789
BauI CACGAG 1 cut(s) 23
BbsI GAAGAC 3 cut(s) 510, 532, 921
Bbv12I GWGCWC 3 cut(s) 530, 789, 825
BbvI GCAGC 6 cut(s) 247, 345, 420, 676, 742, 770
BccI CCATC 5 cut(s) 80, 262, 334, 614, 770
BceAI ACGGC 2 cut(s) 80, 620
BciT130I CCWGG 2 cut(s) 380, 400
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 3 cut(s) 303, 623, 1193
BcoDI GTCTC 5 cut(s) 69, 170, 255, 283, 1199
BfaI CTAG 2 cut(s) 1029, 1216
BfmI CTRYAG 3 cut(s) 591, 756, 993
BglII AGATCT 1 cut(s) 714
BisI GCNGC 7 cut(s) 184, 261, 334, 409, 665, 756, 759
BlsI GCNGC 7 cut(s) 185, 262, 335, 410, 666, 757, 760
Bme1390I CCNGG 5 cut(s) 303, 380, 400, 623, 1193
Bme18I GGWCC 2 cut(s) 111, 812
BmeT110I CYCGRG 1 cut(s) 659
BmgBI CACGTC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 111, 812
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 5 cut(s) 303, 380, 400, 623, 1193
BmsI GCATC 2 cut(s) 82, 588
BoxI GACNNNNGTC 1 cut(s) 39
BpiI GAAGAC 3 cut(s) 510, 532, 921
BplI GAGNNNNNCTC 2 cut(s) 944, 976
BpuEI CTTGAG 1 cut(s) 127
BpuMI CCSGG 3 cut(s) 303, 623, 1193
BsaHI GRCGYC 1 cut(s) 213
BsaI GGTCTC 2 cut(s) 69, 1199
BsaJI CCNNGG 3 cut(s) 114, 364, 621
BsaXI ACNNNNNCTCC 6 cut(s) 140, 170, 602, 632, 848, 878
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 379, 622
Bse118I RCCGGY 3 cut(s) 52, 170, 1172
Bse1I ACTGG 1 cut(s) 178
BseBI CCWGG 2 cut(s) 380, 400
BseDI CCNNGG 3 cut(s) 114, 364, 621
BseGI GGATG 3 cut(s) 241, 1063, 1144
BseLI CCNNNNNNNGG 3 cut(s) 43, 379, 622
BseMII CTCAG 5 cut(s) 515, 665, 687, 1000, 1038
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 968
BseXI GCAGC 6 cut(s) 247, 345, 420, 676, 742, 770
Bsh1285I CGRYCG 1 cut(s) 534
BshFI GGCC 5 cut(s) 347, 455, 605, 734, 1052
BshNI GGYRCC 1 cut(s) 271
BsiEI CGRYCG 1 cut(s) 534
BsiHKAI GWGCWC 3 cut(s) 530, 789, 825
BsiHKCI CYCGRG 1 cut(s) 659
BsiSI CCGG 6 cut(s) 53, 171, 302, 623, 1173, 1192
BslI CCNNNNNNNGG 3 cut(s) 43, 379, 622
BsmAI GTCTC 5 cut(s) 69, 170, 255, 283, 1199
BsmBI CGTCTC 2 cut(s) 255, 283
BsnI GGCC 5 cut(s) 347, 455, 605, 734, 1052
Bso31I GGTCTC 2 cut(s) 69, 1199
BsoBI CYCGRG 1 cut(s) 659
Bsp1286I GDGCHC 3 cut(s) 530, 789, 825
Bsp143I GATC 6 cut(s) 352, 531, 705, 714, 828, 1212
BspACI CCGC 4 cut(s) 78, 184, 633, 780
BspANI GGCC 5 cut(s) 347, 455, 605, 734, 1052
BspCNI CTCAG 5 cut(s) 516, 664, 686, 999, 1039
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 2 cut(s) 760, 997
BspT107I GGYRCC 1 cut(s) 271
BspTNI GGTCTC 2 cut(s) 69, 1199
BsrFI RCCGGY 3 cut(s) 52, 170, 1172
BsrI ACTGG 1 cut(s) 178
BssAI RCCGGY 3 cut(s) 52, 170, 1172
BssECI CCNNGG 3 cut(s) 114, 364, 621
BssMI GATC 6 cut(s) 352, 531, 705, 714, 828, 1212
BssNI GRCGYC 1 cut(s) 213
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 364
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 2 cut(s) 380, 400
Bst4CI ACNGT 6 cut(s) 85, 310, 429, 476, 692, 806
BstACI GRCGYC 1 cut(s) 213
BstC8I GCNNGC 8 cut(s) 50, 54, 172, 345, 491, 555, 628, 736
BstDEI CTNAG 6 cut(s) 231, 524, 651, 673, 986, 1047
BstF5I GGATG 3 cut(s) 241, 1063, 1144
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 6 cut(s) 355, 534, 708, 717, 831, 1215
BstMAI GTCTC 5 cut(s) 69, 170, 255, 283, 1199
BstMBI GATC 6 cut(s) 352, 531, 705, 714, 828, 1212
BstMCI CGRYCG 1 cut(s) 534
BstMWI GCNNNNNNNGC 7 cut(s) 86, 101, 180, 269, 322, 632, 764
BstNI CCWGG 2 cut(s) 380, 400
BstNSI RCATGY 1 cut(s) 1180
BstPAI GACNNNNGTC 1 cut(s) 39
BstSCI CCNGG 5 cut(s) 301, 378, 398, 621, 1191
BstSFI CTRYAG 3 cut(s) 591, 756, 993
BstV1I GCAGC 6 cut(s) 247, 345, 420, 676, 742, 770
BstV2I GAAGAC 3 cut(s) 510, 532, 921
BstX2I RGATCY 1 cut(s) 714
BstYI RGATCY 1 cut(s) 714
BsuRI GGCC 5 cut(s) 347, 455, 605, 734, 1052
BtgZI GCGATG 1 cut(s) 748
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 3 cut(s) 241, 1063, 1144
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 4 cut(s) 12, 185, 681, 697
Cac8I GCNNGC 8 cut(s) 50, 54, 172, 345, 491, 555, 628, 736
CaiI CAGNNNCTG 3 cut(s) 138, 379, 680
CfoI GCGC 1 cut(s) 265
Cfr10I RCCGGY 3 cut(s) 52, 170, 1172
Cfr13I GGNCC 2 cut(s) 111, 812
CseI GACGC 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 378
Csp6I GTAC 1 cut(s) 386
CviAII CATG 5 cut(s) 205, 550, 790, 1177, 1210
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 6 cut(s) 231, 524, 651, 673, 986, 1047
DpnI GATC 6 cut(s) 354, 533, 707, 716, 830, 1214
DpnII GATC 6 cut(s) 352, 531, 705, 714, 828, 1212
EaeI YGGCCR 2 cut(s) 453, 603
EciI GGCGGA 2 cut(s) 67, 622
Ecl136II GAGCTC 2 cut(s) 528, 787
Eco130I CCWWGG 1 cut(s) 364
Eco24I GRGCYC 2 cut(s) 530, 789
Eco31I GGTCTC 2 cut(s) 69, 1199
Eco47I GGWCC 2 cut(s) 111, 812
Eco53kI GAGCTC 2 cut(s) 528, 787
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 659
EcoICRI GAGCTC 2 cut(s) 528, 787
EcoO109I RGGNCCY 1 cut(s) 111
EcoRI GAATTC 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 378, 398
EcoT14I CCWWGG 1 cut(s) 364
EcoT38I GRGCYC 2 cut(s) 530, 789
ErhI CCWWGG 1 cut(s) 364
Esp3I CGTCTC 2 cut(s) 255, 283
FaeI CATG 5 cut(s) 208, 553, 793, 1180, 1213
FalI AAGNNNNNCTT 2 cut(s) 397, 429
FatI CATG 5 cut(s) 204, 549, 789, 1176, 1209
FbaI TGATCA 1 cut(s) 828
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 7 cut(s) 184, 261, 334, 409, 665, 756, 759
FokI GGATG 3 cut(s) 248, 1050, 1151
FriOI GRGCYC 2 cut(s) 530, 789
Fsp4HI GCNGC 7 cut(s) 184, 261, 334, 409, 665, 756, 759
FspBI CTAG 2 cut(s) 1029, 1216
GlaI GCGC 1 cut(s) 264
GluI GCNGC 7 cut(s) 184, 261, 334, 409, 665, 756, 759
HaeIII GGCC 5 cut(s) 347, 455, 605, 734, 1052
HapII CCGG 6 cut(s) 53, 171, 302, 623, 1173, 1192
HgaI GACGC 1 cut(s) 124
HhaI GCGC 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 5 cut(s) 208, 553, 793, 1180, 1213
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 7 cut(s) 163, 298, 516, 569, 746, 956, 1093
HpaII CCGG 6 cut(s) 53, 171, 302, 623, 1173, 1192
HphI GGTGA 2 cut(s) 722, 1048
Hpy166II GTNNAC 3 cut(s) 211, 688, 1117
Hpy188I TCNGA 8 cut(s) 34, 118, 283, 396, 515, 525, 949, 1048
Hpy188III TCNNGA 3 cut(s) 289, 479, 832
Hpy8I GTNNAC 3 cut(s) 211, 688, 1117
Hpy99I CGWCG 4 cut(s) 89, 215, 323, 928
HpyAV CCTTC 5 cut(s) 190, 262, 530, 847, 1035
HpyCH4III ACNGT 6 cut(s) 85, 310, 429, 476, 692, 806
HpyCH4IV ACGT 3 cut(s) 213, 318, 507
HpyCH4V TGCA 8 cut(s) 147, 204, 316, 333, 343, 758, 911, 995
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 101, 180, 269, 322, 632, 764
HpyF3I CTNAG 6 cut(s) 231, 524, 651, 673, 986, 1047
HpySE526I ACGT 3 cut(s) 213, 318, 507
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 5 cut(s) 208, 553, 793, 1180, 1213
HspAI GCGC 1 cut(s) 263
KroI GCCGGC 2 cut(s) 52, 170
KroNI GCCGGC 2 cut(s) 54, 172
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 6 cut(s) 352, 531, 705, 714, 828, 1212
LmnI GCTCC 1 cut(s) 635
Lsp1109I GCAGC 6 cut(s) 247, 345, 420, 676, 742, 770
LweI GCATC 2 cut(s) 82, 588
MabI ACCWGGT 1 cut(s) 378
MaeI CTAG 2 cut(s) 1029, 1216
MaeII ACGT 3 cut(s) 213, 318, 507
MaeIII GTNAC 4 cut(s) 654, 740, 838, 1162
MalI GATC 6 cut(s) 354, 533, 707, 716, 830, 1214
MboI GATC 6 cut(s) 352, 531, 705, 714, 828, 1212
MflI RGATCY 1 cut(s) 714
MhlI GDGCHC 3 cut(s) 530, 789, 825
MlsI TGGCCA 2 cut(s) 455, 605
MluCI AATT 5 cut(s) 357, 390, 482, 883, 897
MluNI TGGCCA 2 cut(s) 455, 605
MlyI GAGTC 4 cut(s) 172, 563, 755, 965
Mox20I TGGCCA 2 cut(s) 455, 605
MreI CGCCGGCG 1 cut(s) 170
MroNI GCCGGC 2 cut(s) 52, 170
MscI TGGCCA 2 cut(s) 455, 605
MseI TTAA 4 cut(s) 17, 416, 866, 900
Msp20I TGGCCA 2 cut(s) 455, 605
MspA1I CMGCKG 2 cut(s) 186, 643
MspI CCGG 6 cut(s) 53, 171, 302, 623, 1173, 1192
MspR9I CCNGG 5 cut(s) 303, 380, 400, 623, 1193
MvaI CCWGG 2 cut(s) 380, 400
MwoI GCNNNNNNNGC 7 cut(s) 86, 101, 180, 269, 322, 632, 764
NaeI GCCGGC 2 cut(s) 54, 172
NciI CCSGG 3 cut(s) 303, 623, 1193
NdeII GATC 6 cut(s) 352, 531, 705, 714, 828, 1212
NgoMIV GCCGGC 2 cut(s) 52, 170
NlaIII CATG 5 cut(s) 208, 553, 793, 1180, 1213
NlaIV GGNNCC 1 cut(s) 273
NmeAIII GCCGAG 2 cut(s) 555, 1004
NmuCI GTSAC 4 cut(s) 654, 740, 838, 1162
NspI RCATGY 1 cut(s) 1180
PaeR7I CTCGAG 1 cut(s) 659
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PdiI GCCGGC 2 cut(s) 54, 172
PfeI GAWTC 3 cut(s) 298, 516, 1093
PflMI CCANNNNNTGG 1 cut(s) 379
PfoI TCCNGGA 2 cut(s) 301, 1191
PkrI GCNGC 7 cut(s) 185, 262, 335, 410, 666, 757, 760
Ple19I CGATCG 1 cut(s) 534
PleI GAGTC 4 cut(s) 171, 563, 754, 964
PpsI GAGTC 4 cut(s) 171, 563, 754, 964
PpuMI RGGWCCY 1 cut(s) 111
PshAI GACNNNNGTC 1 cut(s) 39
PshBI ATTAAT 1 cut(s) 900
Psp124BI GAGCTC 2 cut(s) 530, 789
Psp5II RGGWCCY 1 cut(s) 111
Psp6I CCWGG 2 cut(s) 378, 398
PspGI CCWGG 2 cut(s) 378, 398
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 2 cut(s) 111, 812
PspPPI RGGWCCY 1 cut(s) 111
PspXI VCTCGAGB 1 cut(s) 659
PstI CTGCAG 2 cut(s) 760, 997
PstNI CAGNNNCTG 3 cut(s) 138, 379, 680
PsuI RGATCY 1 cut(s) 714
PvuI CGATCG 1 cut(s) 534
PvuII CAGCTG 1 cut(s) 643
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SacI GAGCTC 2 cut(s) 530, 789
SalI GTCGAC 1 cut(s) 209
SaqAI TTAA 4 cut(s) 17, 416, 866, 900
SatI GCNGC 7 cut(s) 184, 261, 334, 409, 665, 756, 759
Sau3AI GATC 6 cut(s) 352, 531, 705, 714, 828, 1212
Sau96I GGNCC 2 cut(s) 111, 812
SchI GAGTC 4 cut(s) 172, 563, 755, 965
ScrFI CCNGG 5 cut(s) 303, 380, 400, 623, 1193
SduI GDGCHC 3 cut(s) 530, 789, 825
SexAI ACCWGGT 1 cut(s) 378
SfaNI GCATC 2 cut(s) 82, 588
SfcI CTRYAG 3 cut(s) 591, 756, 993
Sfr274I CTCGAG 1 cut(s) 659
SgrAI CRCCGGYG 1 cut(s) 170
SinI GGWCC 2 cut(s) 111, 812
SlaI CTCGAG 1 cut(s) 659
SmlI CTYRAG 2 cut(s) 106, 659
SmoI CTYRAG 2 cut(s) 106, 659
Sse9I AATT 5 cut(s) 357, 390, 482, 883, 897
SsiI CCGC 4 cut(s) 78, 184, 633, 780
SspMI CTAG 2 cut(s) 1029, 1216
SstI GAGCTC 2 cut(s) 530, 789
StyD4I CCNGG 5 cut(s) 301, 378, 398, 621, 1191
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 6 cut(s) 85, 310, 429, 476, 692, 806
TaiI ACGT 3 cut(s) 216, 321, 510
TaqI TCGA 5 cut(s) 210, 321, 530, 567, 660
TasI AATT 5 cut(s) 357, 390, 482, 883, 897
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 3 cut(s) 298, 516, 1093
Tru1I TTAA 4 cut(s) 17, 416, 866, 900
Tru9I TTAA 4 cut(s) 17, 416, 866, 900
TscAI CASTG 4 cut(s) 12, 185, 681, 697
TseFI GTSAC 4 cut(s) 654, 740, 838, 1162
TseI GCWGC 6 cut(s) 260, 333, 408, 664, 755, 758
Tsp45I GTSAC 4 cut(s) 654, 740, 838, 1162
TspDTI ATGAA 2 cut(s) 118, 933
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 4 cut(s) 12, 185, 681, 697
Van91I CCANNNNNTGG 1 cut(s) 379
VpaK11BI GGWCC 2 cut(s) 111, 812
VspI ATTAAT 1 cut(s) 900
XapI RAATTY 1 cut(s) 482
XceI RCATGY 1 cut(s) 1180
XhoI CTCGAG 1 cut(s) 659
XmiI GTMKAC 1 cut(s) 210
XspI CTAG 2 cut(s) 1029, 1216
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.