AT5G53750

CBS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003827
Physical Location & Seq
Forward (+)
21817128 .. 21818958
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53750.1

Sequence Viewer

Length: 1227 bp
ATGGCATTAACCCTTTTGTCTCACGAATTATCTGACCTCTGTATCGGTAAGCCACCTTTACGGTGTCTCTCCGTCGCCACAGCCACCGTAGCTGACGCCATCGCCGCTCTCAAATCCTCTGACGAACCGTTCCTCACCGTATGGAGCTGTAATCACGATGAGAAAACAGATGATAATGATAAGTGTGAGTGTTTGGGTAAGATCTGTATGGCTGATGTAATCTGTTACCTATCCAAATTCGACAACAATGTTTTGTCTCTTTCCTCTGCTTTCGACGCATCTGTCTCTGTTCTTCTTCCCAAATCTCGTGCCCTCGTCGTCCATGTTCAATCTTCTTGCAGTTTGATTGAAGCTATTGATCTGATAATCAAAGGAGCACAGAATCTGATTGTTCCGATTCATACGAAATCAATCACAAAGAGAAGACAACAACAAAAACTTCTGAAACGAAACGTCGTCGTTTCACTCACCAACGCAACTTCAACAACCCACAAAAACAGCCGAGAATTCTGCTGGATCACACAAGAAGACATTATTCGATTCCTTCTCGATTCCATTAGCGTTTTCTCTCCATTACCGTCGCTTTCTATCTCCGATCTTGGAGTTATCAATAGTACACACACTATCCTCGCCGTTGATTACTACTCCTCAGCTGCTTCCGCCGTCTCTGCCATCTCTCGTGCCATCTTGGACAATGTCTCTGTCGCGGTGGTTGGTAAAGGATGTGATCAAGAAGATCCATGTATGGTTTTGATAGGCGAGATTTCACCGATGACACTCGCTTGCTGCGATGAAACTGCCGTAGCAGCGGTTGCTACACTCTCTGCCGGAGATTTAATGTCCTATATCGACGGTAGTGGTCCGCCGGAGAGTCTAGTTGGAGTAGTTAGGAATCGTTTGGAAGATAAAGGGATGGTTGGATTAATCTCACTCATTGATTCTTTGTCGTTGTCGTCGGGGTCTTCCTCGGATGAAGAATCTCCGGCGGGGAAGACGAGAATGACTTCTTCGTATGGGAGGTCGGTGAGTAGCGCGGCGAGGATGGCTAGGAAATCGGTGGCGATAGTGTGTAATCGGAAGAGTTCTTTAATGGCGGTGATGATACAAGCTATTGCTCATAGAGTGAGTTATGTGTGGGTGATTGATGAAGATGGTTGTTTGATTGGTATGGTTACTTTTGTTGATATATTGAAACTTTTTAGGGAATTTTTGGATGATGAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS

Protein Analysis

408

Amino Acids

43.79

Weight (kDa)

5.25

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AccBSI CCGCTC 1 cut(s) 107
AccII CGCG 2 cut(s) 707, 1034
AciI CCGC 8 cut(s) 105, 660, 707, 809, 863, 986, 1034, 1094
AclWI GGATC 2 cut(s) 524, 731
AcsI RAATTY 3 cut(s) 236, 506, 1205
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 1 cut(s) 616
AgsI TTSAA 4 cut(s) 329, 350, 483, 1192
AluBI AGCT 5 cut(s) 92, 147, 353, 653, 1109
AluI AGCT 5 cut(s) 92, 147, 353, 653, 1109
Alw21I GWGCWC 1 cut(s) 379
Alw26I GTCTC 6 cut(s) 24, 71, 261, 289, 670, 703
AlwI GGATC 2 cut(s) 524, 731
AlwNI CAGNNNCTG 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 653, 786, 806
ApoI RAATTY 3 cut(s) 236, 506, 1205
AseI ATTAAT 1 cut(s) 923
Asp700I GAANNNNTTC 1 cut(s) 1003
AspLEI GCGC 1 cut(s) 1034
AspS9I GGNCC 1 cut(s) 860
AsuHPI GGTGA 6 cut(s) 127, 460, 759, 1036, 1108, 1150
AvaII GGWCC 1 cut(s) 860
BaeGI GKGCMC 1 cut(s) 313
BaeI ACNNNNGTAYC 2 cut(s) 25, 58
BauI CACGAG 2 cut(s) 306, 678
BbsI GAAGAC 4 cut(s) 430, 534, 954, 998
Bbv12I GWGCWC 1 cut(s) 379
BbvCI CCTCAGC 1 cut(s) 649
BbvI GCAGC 3 cut(s) 640, 773, 818
BccI CCATC 6 cut(s) 107, 680, 692, 907, 1036, 1145
BceAI ACGGC 3 cut(s) 617, 647, 785
BcgI CGANNNNNNTGC 4 cut(s) 492, 526, 779, 813
BclI TGATCA 1 cut(s) 727
BcoDI GTCTC 6 cut(s) 24, 71, 261, 289, 670, 703
BfaI CTAG 2 cut(s) 875, 1047
BglII AGATCT 1 cut(s) 201
BisI GCNGC 5 cut(s) 105, 654, 787, 807, 1035
BlsI GCNGC 5 cut(s) 106, 655, 788, 808, 1036
Bme18I GGWCC 1 cut(s) 860
BmgT120I GGNCC 1 cut(s) 860
BmsI GCATC 1 cut(s) 287
BpiI GAAGAC 4 cut(s) 430, 534, 954, 998
Bpu10I CCTNAGC 1 cut(s) 649
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 966
BseDI CCNNGG 1 cut(s) 966
BseGI GGATG 5 cut(s) 728, 918, 976, 1047, 1219
BseMII CTCAG 1 cut(s) 663
BseRI GAGGAG 1 cut(s) 637
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 3 cut(s) 640, 773, 818
Bsh1236I CGCG 2 cut(s) 707, 1034
BsiHKAI GWGCWC 1 cut(s) 379
BsiSI CCGG 3 cut(s) 828, 866, 983
BsmAI GTCTC 6 cut(s) 24, 71, 261, 289, 670, 703
BsmBI CGTCTC 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 313, 379
Bsp143I GATC 6 cut(s) 201, 358, 516, 595, 727, 736
BspACI CCGC 8 cut(s) 105, 660, 707, 809, 863, 986, 1034, 1094
BspCNI CTCAG 1 cut(s) 662
BspFNI CGCG 2 cut(s) 707, 1034
BspPI GGATC 2 cut(s) 524, 731
BsrBI CCGCTC 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 966
BssMI GATC 6 cut(s) 201, 358, 516, 595, 727, 736
BssNI GRCGYC 1 cut(s) 96
BssSI CACGAG 2 cut(s) 306, 678
Bst2BI CACGAG 2 cut(s) 306, 678
Bst4CI ACNGT 6 cut(s) 63, 88, 129, 139, 579, 854
Bst6I CTCTTC 1 cut(s) 1073
BstACI GRCGYC 1 cut(s) 96
BstAPI GCANNNNNTGC 1 cut(s) 812
BstC8I GCNNGC 1 cut(s) 784
BstDEI CTNAG 1 cut(s) 649
BstF5I GGATG 5 cut(s) 728, 918, 976, 1047, 1219
BstFNI CGCG 2 cut(s) 707, 1034
BstHHI GCGC 1 cut(s) 1034
BstKTI GATC 6 cut(s) 204, 361, 519, 598, 730, 739
BstMAI GTCTC 6 cut(s) 24, 71, 261, 289, 670, 703
BstMBI GATC 6 cut(s) 201, 358, 516, 595, 727, 736
BstMWI GCNNNNNNNGC 8 cut(s) 89, 104, 275, 659, 668, 806, 812, 1043
BstSLI GKGCMC 1 cut(s) 313
BstUI CGCG 2 cut(s) 707, 1034
BstV1I GCAGC 3 cut(s) 640, 773, 818
BstV2I GAAGAC 4 cut(s) 430, 534, 954, 998
BstX2I RGATCY 2 cut(s) 201, 736
BstYI RGATCY 2 cut(s) 201, 736
BtgZI GCGATG 2 cut(s) 85, 804
BtsCI GGATG 5 cut(s) 728, 918, 976, 1047, 1219
Cac8I GCNNGC 1 cut(s) 784
CaiI CAGNNNCTG 1 cut(s) 385
CfoI GCGC 1 cut(s) 1034
Cfr13I GGNCC 1 cut(s) 860
CseI GACGC 2 cut(s) 104, 284
Csp6I GTAC 1 cut(s) 615
CviAII CATG 2 cut(s) 323, 741
CviQI GTAC 1 cut(s) 615
DdeI CTNAG 1 cut(s) 649
DpnI GATC 6 cut(s) 203, 360, 518, 597, 729, 738
DpnII GATC 6 cut(s) 201, 358, 516, 595, 727, 736
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 1073
EarI CTCTTC 1 cut(s) 1073
EciI GGCGGA 2 cut(s) 649, 852
Eco47I GGWCC 1 cut(s) 860
EcoRI GAATTC 1 cut(s) 506
Esp3I CGTCTC 1 cut(s) 670
FaeI CATG 2 cut(s) 326, 744
FatI CATG 2 cut(s) 322, 740
FauI CCCGC 1 cut(s) 979
FbaI TGATCA 1 cut(s) 727
Fnu4HI GCNGC 5 cut(s) 105, 654, 787, 807, 1035
FokI GGATG 4 cut(s) 735, 925, 983, 1054
Fsp4HI GCNGC 5 cut(s) 105, 654, 787, 807, 1035
FspBI CTAG 2 cut(s) 875, 1047
GlaI GCGC 1 cut(s) 1033
GluI GCNGC 5 cut(s) 105, 654, 787, 807, 1035
HapII CCGG 3 cut(s) 828, 866, 983
HgaI GACGC 2 cut(s) 104, 284
HhaI GCGC 1 cut(s) 1034
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 2 cut(s) 326, 744
Hin6I GCGC 1 cut(s) 1032
HinP1I GCGC 1 cut(s) 1032
HinfI GANTC 8 cut(s) 382, 397, 540, 551, 871, 892, 938, 977
HpaII CCGG 3 cut(s) 828, 866, 983
HphI GGTGA 6 cut(s) 127, 460, 759, 1036, 1108, 1150
Hpy166II GTNNAC 1 cut(s) 617
Hpy188I TCNGA 9 cut(s) 34, 121, 363, 387, 396, 444, 595, 970, 1077
Hpy188III TCNNGA 4 cut(s) 23, 155, 548, 731
Hpy8I GTNNAC 1 cut(s) 617
Hpy99I CGWCG 8 cut(s) 77, 278, 320, 458, 461, 583, 854, 958
HpyAV CCTTC 1 cut(s) 554
HpyCH4III ACNGT 6 cut(s) 63, 88, 129, 139, 579, 854
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 1 cut(s) 339
HpyF10VI GCNNNNNNNGC 8 cut(s) 89, 104, 275, 659, 668, 806, 812, 1043
HpyF3I CTNAG 1 cut(s) 649
HpySE526I ACGT 1 cut(s) 453
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 2 cut(s) 326, 744
HspAI GCGC 1 cut(s) 1032
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 6 cut(s) 201, 358, 516, 595, 727, 736
LmnI GCTCC 2 cut(s) 144, 374
LpnPI CCDG 4 cut(s) 499, 841, 879, 996
Lsp1109I GCAGC 3 cut(s) 640, 773, 818
LweI GCATC 1 cut(s) 287
MaeI CTAG 2 cut(s) 875, 1047
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 2 cut(s) 224, 1171
MalI GATC 6 cut(s) 203, 360, 518, 597, 729, 738
MbiI CCGCTC 1 cut(s) 107
MboI GATC 6 cut(s) 201, 358, 516, 595, 727, 736
MflI RGATCY 2 cut(s) 201, 736
MhlI GDGCHC 2 cut(s) 313, 379
MluCI AATT 4 cut(s) 26, 236, 506, 1205
MlyI GAGTC 1 cut(s) 880
MmeI TCCRAC 2 cut(s) 859, 898
MroXI GAANNNNTTC 1 cut(s) 1003
MseI TTAA 4 cut(s) 8, 836, 923, 1088
MspA1I CMGCKG 2 cut(s) 653, 809
MspI CCGG 3 cut(s) 828, 866, 983
MvnI CGCG 2 cut(s) 707, 1034
MwoI GCNNNNNNNGC 8 cut(s) 89, 104, 275, 659, 668, 806, 812, 1043
NdeII GATC 6 cut(s) 201, 358, 516, 595, 727, 736
NlaIII CATG 2 cut(s) 326, 744
NmeAIII GCCGAG 1 cut(s) 527
PcsI WCGNNNNNNNCGW 1 cut(s) 786
PdmI GAANNNNTTC 1 cut(s) 1003
PfeI GAWTC 7 cut(s) 382, 397, 540, 551, 892, 938, 977
PflFI GACNNNGTC 1 cut(s) 695
PkrI GCNGC 5 cut(s) 106, 655, 788, 808, 1036
PleI GAGTC 1 cut(s) 879
PpsI GAGTC 1 cut(s) 879
PshBI ATTAAT 1 cut(s) 923
PspPI GGNCC 1 cut(s) 860
PstNI CAGNNNCTG 1 cut(s) 385
PsuI RGATCY 2 cut(s) 201, 736
PsyI GACNNNGTC 1 cut(s) 695
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 1 cut(s) 616
RsaNI GTAC 1 cut(s) 615
SaqAI TTAA 4 cut(s) 8, 836, 923, 1088
SatI GCNGC 5 cut(s) 105, 654, 787, 807, 1035
Sau3AI GATC 6 cut(s) 201, 358, 516, 595, 727, 736
Sau96I GGNCC 1 cut(s) 860
SchI GAGTC 1 cut(s) 880
SduI GDGCHC 2 cut(s) 313, 379
SfaNI GCATC 1 cut(s) 287
SinI GGWCC 1 cut(s) 860
Sse9I AATT 4 cut(s) 26, 236, 506, 1205
SsiI CCGC 8 cut(s) 105, 660, 707, 809, 863, 986, 1034, 1094
SspMI CTAG 2 cut(s) 875, 1047
TaaI ACNGT 6 cut(s) 63, 88, 129, 139, 579, 854
TaiI ACGT 1 cut(s) 456
TaqI TCGA 5 cut(s) 240, 273, 538, 549, 849
TasI AATT 4 cut(s) 26, 236, 506, 1205
TatI WGTACW 1 cut(s) 614
TauI GCSGC 2 cut(s) 107, 1037
TfiI GAWTC 7 cut(s) 382, 397, 540, 551, 892, 938, 977
Tru1I TTAA 4 cut(s) 8, 836, 923, 1088
Tru9I TTAA 4 cut(s) 8, 836, 923, 1088
TseI GCWGC 3 cut(s) 653, 786, 806
TspDTI ATGAA 4 cut(s) 389, 807, 987, 1161
TspGWI ACGGA 1 cut(s) 61
Tth111I GACNNNGTC 1 cut(s) 695
VpaK11BI GGWCC 1 cut(s) 860
VspI ATTAAT 1 cut(s) 923
XapI RAATTY 3 cut(s) 236, 506, 1205
XmnI GAANNNNTTC 1 cut(s) 1003
XspI CTAG 2 cut(s) 875, 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.