AT4G29850

Eukaryotic protein of unknown function (DUF872)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002326
Physical Location & Seq
Reverse (-)
14601621 .. 14603011
1391 bp
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UTR
Exon/CDS
Intron
AT4G29850.1

Sequence Viewer

Length: 312 bp
ATGGCGTACGTCGACCATGCGTTCTCGATAACGGACGAGGACATTATGATTGAGACTTCATACACCGTCAATAACCGACCGCCGGTGAAGGAGATCGCACTCGCGGTGGCTTTACTCGTGTTCGGAACCCTAGGAATTGTCTCCGGTTTCTTCATGGCTTATAATCGAGTTGGCGGTGATCGAGGCCACGGGATTTTCTTCATCGTCCTCGGGTGTCTTCTGTTCATTCCCGGGTTTTACTATACACGGATTGCGTATTATGCTTACAAAGGATACAAAGGTTTCTCCTTCTCAAACATACCGCCCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF872

Protein Analysis

103

Amino Acids

11.48

Weight (kDa)

6.7

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM_230_134 PF05915 29 - 101 2.1e-17 Transmembrane proteins 230/134
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
AccI GTMKAC 1 cut(s) 12
AccII CGCG 1 cut(s) 104
AciI CCGC 4 cut(s) 80, 104, 174, 302
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 1 cut(s) 82
Alw26I GTCTC 2 cut(s) 47, 145
Ama87I CYCGRG 2 cut(s) 209, 230
AoxI GGCC 1 cut(s) 184
AspA2I CCTAGG 1 cut(s) 130
AsuC2I CCSGG 2 cut(s) 231, 232
AsuHPI GGTGA 2 cut(s) 97, 188
AvaI CYCGRG 2 cut(s) 209, 230
AvrII CCTAGG 1 cut(s) 130
BaeI ACNNNNGTAYC 2 cut(s) 265, 298
BauI CACGAG 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 209
BciVI GTATCC 1 cut(s) 266
BcnI CCSGG 2 cut(s) 231, 232
BcoDI GTCTC 2 cut(s) 47, 145
BfaI CTAG 1 cut(s) 131
BfuI GTATCC 1 cut(s) 266
BlnI CCTAGG 1 cut(s) 130
Bme1390I CCNGG 2 cut(s) 231, 232
BmeT110I CYCGRG 2 cut(s) 209, 230
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 231, 232
BpiI GAAGAC 1 cut(s) 209
BpuMI CCSGG 2 cut(s) 231, 232
BsaJI CCNNGG 4 cut(s) 130, 187, 208, 230
BsaWI WCCGGW 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse118I RCCGGY 1 cut(s) 82
BseDI CCNNGG 4 cut(s) 130, 187, 208, 230
BseLI CCNNNNNNNGG 1 cut(s) 82
Bsh1236I CGCG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 80
BshFI GGCC 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 80
BsiHKCI CYCGRG 2 cut(s) 209, 230
BsiSI CCGG 3 cut(s) 83, 144, 231
BsiWI CGTACG 1 cut(s) 6
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 2 cut(s) 47, 145
BsnI GGCC 1 cut(s) 186
BsoBI CYCGRG 2 cut(s) 209, 230
Bsp143I GATC 2 cut(s) 93, 178
BspACI CCGC 4 cut(s) 80, 104, 174, 302
BspANI GGCC 1 cut(s) 186
BspFNI CGCG 1 cut(s) 104
BspLI GGNNCC 1 cut(s) 127
BsrFI RCCGGY 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 4 cut(s) 130, 187, 208, 230
BssMI GATC 2 cut(s) 93, 178
BssSI CACGAG 1 cut(s) 116
BssT1I CCWWGG 1 cut(s) 130
Bst2BI CACGAG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 187
BstFNI CGCG 1 cut(s) 104
BstKTI GATC 2 cut(s) 96, 181
BstMAI GTCTC 2 cut(s) 47, 145
BstMBI GATC 2 cut(s) 93, 178
BstMCI CGRYCG 1 cut(s) 80
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 229, 230
BstUI CGCG 1 cut(s) 104
BstV2I GAAGAC 1 cut(s) 209
BsuI GTATCC 1 cut(s) 266
BsuRI GGCC 1 cut(s) 186
BtgI CCRYGG 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 82
Cfr9I CCCGGG 1 cut(s) 230
Csp6I GTAC 1 cut(s) 7
CviAII CATG 2 cut(s) 17, 154
CviJI RGCY 3 cut(s) 110, 158, 186
CviKI_1 RGCY 3 cut(s) 110, 158, 186
CviQI GTAC 1 cut(s) 7
DpnI GATC 2 cut(s) 95, 180
DpnII GATC 2 cut(s) 93, 178
Eco130I CCWWGG 1 cut(s) 130
Eco88I CYCGRG 2 cut(s) 209, 230
EcoT14I CCWWGG 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 2 cut(s) 20, 157
FaiI YATR 8 cut(s) 18, 47, 61, 155, 162, 243, 261, 299
FatI CATG 2 cut(s) 16, 153
FblI GTMKAC 1 cut(s) 12
FspBI CTAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 186
HapII CCGG 3 cut(s) 83, 144, 231
Hin1II CATG 2 cut(s) 20, 157
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HpaII CCGG 3 cut(s) 83, 144, 231
HphI GGTGA 2 cut(s) 97, 188
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 1 cut(s) 125
Hpy188III TCNNGA 1 cut(s) 25
Hpy8I GTNNAC 1 cut(s) 13
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 2 cut(s) 82, 298
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 9
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 2 cut(s) 20, 157
Kzo9I GATC 2 cut(s) 93, 178
LpnPI CCDG 3 cut(s) 96, 157, 244
MaeI CTAG 1 cut(s) 131
MaeII ACGT 1 cut(s) 9
MalI GATC 2 cut(s) 95, 180
MboI GATC 2 cut(s) 93, 178
MboII GAAGA 3 cut(s) 142, 190, 209
MluCI AATT 1 cut(s) 135
MnlI CCTC 3 cut(s) 31, 176, 218
MspI CCGG 3 cut(s) 83, 144, 231
MspR9I CCNGG 2 cut(s) 231, 232
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 1 cut(s) 260
NciI CCSGG 2 cut(s) 231, 232
NdeII GATC 2 cut(s) 93, 178
NlaIII CATG 2 cut(s) 20, 157
NlaIV GGNNCC 1 cut(s) 127
Pfl23II CGTACG 1 cut(s) 6
PsiI TTATAA 1 cut(s) 162
PspLI CGTACG 1 cut(s) 6
PspN4I GGNNCC 1 cut(s) 127
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SalI GTCGAC 1 cut(s) 11
Sau3AI GATC 2 cut(s) 93, 178
ScrFI CCNGG 2 cut(s) 231, 232
SetI ASST 2 cut(s) 12, 283
SgrAI CRCCGGYG 1 cut(s) 82
SmaI CCCGGG 1 cut(s) 232
Sse9I AATT 1 cut(s) 135
SsiI CCGC 4 cut(s) 80, 104, 174, 302
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 229, 230
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 12
TaqI TCGA 4 cut(s) 12, 26, 166, 181
TasI AATT 1 cut(s) 135
TspDTI ATGAA 4 cut(s) 48, 142, 190, 214
TspGWI ACGGA 2 cut(s) 47, 262
TspMI CCCGGG 1 cut(s) 230
XmaI CCCGGG 1 cut(s) 230
XmaJI CCTAGG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.