FvH4_4g06710

Transmembrane protein 230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002326
Physical Location & Seq
Reverse (-)
6015783 .. 6018054
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06710.t4

Sequence Viewer

Length: 312 bp
ATGGCGTATGTAGATCACGCATTTTCCATTACCGACGAAGACATAATGATGGAGACCTCCTACACCGTCAACAACAAACCCCCAATCAAAGAGATCGCTCTCGCCATCGCCCTCCTCGTCACAGGAACCCTCGGCATTGTAATCGGCAGCCTCATGGCCTACAACCACGTCGGCGGTGACAAAGCTCACGGCTTGTTCTTTGCGATTCTGGGTGGGATCCTGTTCATTCCGGGGTCCTATTACACGCGGATTGCTTACTATGCTTACAAAGGTTACAAGGGCTTCTCTTTCTCCAACATACCGCCTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF872

Protein Analysis

104

Amino Acids

11.22

Weight (kDa)

6.23

Isoelectric Point (pI)

22.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM_230_134 PF05915 27 - 101 5.3e-17 Transmembrane proteins 230/134
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 247
AciI CCGC 3 cut(s) 174, 247, 302
AclWI GGATC 2 cut(s) 211, 224
AjiI CACGTC 1 cut(s) 169
AluBI AGCT 1 cut(s) 185
AluI AGCT 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 2 cut(s) 211, 224
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 234
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 234
BamHI GGATCC 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 159
BccI CCATC 2 cut(s) 43, 113
BceAI ACGGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BcnI CCSGG 1 cut(s) 231
BcoDI GTCTC 1 cut(s) 47
BisI GCNGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 234
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 3 cut(s) 127, 218, 235
BmrFI CCNGG 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 130, 230
BseDI CCNNGG 2 cut(s) 130, 230
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 159
Bsh1236I CGCG 1 cut(s) 247
BshFI GGCC 1 cut(s) 158
BsiSI CCGG 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 47
BsnI GGCC 1 cut(s) 158
Bso31I GGTCTC 1 cut(s) 47
Bsp143I GATC 3 cut(s) 13, 93, 216
BspACI CCGC 3 cut(s) 174, 247, 302
BspANI GGCC 1 cut(s) 158
BspFNI CGCG 1 cut(s) 247
BspLI GGNNCC 3 cut(s) 127, 218, 235
BspPI GGATC 2 cut(s) 211, 224
BspTNI GGTCTC 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 130, 230
BssMI GATC 3 cut(s) 13, 93, 216
Bst4CI ACNGT 1 cut(s) 67
BstFNI CGCG 1 cut(s) 247
BstKTI GATC 3 cut(s) 16, 96, 219
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 3 cut(s) 13, 93, 216
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 229
BstUI CGCG 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 1 cut(s) 158
BtgZI GCGATG 1 cut(s) 91
BtrI CACGTC 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 234
CviAII CATG 1 cut(s) 154
CviJI RGCY 5 cut(s) 150, 158, 185, 192, 282
CviKI_1 RGCY 5 cut(s) 150, 158, 185, 192, 282
DpnI GATC 3 cut(s) 15, 95, 218
DpnII GATC 3 cut(s) 13, 93, 216
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 234
EcoO109I RGGNCCY 1 cut(s) 234
FaeI CATG 1 cut(s) 157
FaiI YATR 5 cut(s) 9, 44, 155, 261, 299
FatI CATG 1 cut(s) 153
Fnu4HI GCNGC 1 cut(s) 148
Fsp4HI GCNGC 1 cut(s) 148
GluI GCNGC 1 cut(s) 148
HaeIII GGCC 1 cut(s) 158
HapII CCGG 1 cut(s) 230
Hin1II CATG 1 cut(s) 157
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 1 cut(s) 205
HpaII CCGG 1 cut(s) 230
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 70
Hpy8I GTNNAC 1 cut(s) 70
Hpy99I CGWCG 2 cut(s) 38, 173
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 168
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 1 cut(s) 157
Kzo9I GATC 3 cut(s) 13, 93, 216
LpnPI CCDG 4 cut(s) 108, 194, 233, 243
Lsp1109I GCAGC 1 cut(s) 159
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 3 cut(s) 118, 176, 272
MalI GATC 3 cut(s) 15, 95, 218
MboI GATC 3 cut(s) 13, 93, 216
MboII GAAGA 1 cut(s) 50
MflI RGATCY 1 cut(s) 216
MnlI CCTC 5 cut(s) 67, 122, 125, 140, 161
MslI CAYNNNNRTG 1 cut(s) 47
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 231
MvnI CGCG 1 cut(s) 247
MwoI GCNNNNNNNGC 1 cut(s) 260
NciI CCSGG 1 cut(s) 231
NdeII GATC 3 cut(s) 13, 93, 216
NlaIII CATG 1 cut(s) 157
NlaIV GGNNCC 3 cut(s) 127, 218, 235
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 118, 176
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PfeI GAWTC 1 cut(s) 205
PkrI GCNGC 1 cut(s) 149
PpuMI RGGWCCY 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 234
PspN4I GGNNCC 3 cut(s) 127, 218, 235
PspPI GGNCC 1 cut(s) 234
PspPPI RGGWCCY 1 cut(s) 234
PsuI RGATCY 1 cut(s) 216
RseI CAYNNNNRTG 1 cut(s) 47
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 3 cut(s) 13, 93, 216
Sau96I GGNCC 1 cut(s) 234
ScrFI CCNGG 1 cut(s) 231
SetI ASST 4 cut(s) 59, 171, 187, 274
SinI GGWCC 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 47
SsiI CCGC 3 cut(s) 174, 247, 302
StyD4I CCNGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 171
TfiI GAWTC 1 cut(s) 205
TseFI GTSAC 2 cut(s) 118, 176
TseI GCWGC 1 cut(s) 147
Tsp45I GTSAC 2 cut(s) 118, 176
TspDTI ATGAA 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.