MD13G1237400.v1.1

Transmembrane protein 230-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002326
Physical Location & Seq
Reverse (-)
24148234 .. 24149104
871 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1237400.v1.1.491

Sequence Viewer

Length: 312 bp
ATGGCGTACGTCGATCACGCGTTTTCCATTACAGACGAGGACATGATGATGGAGGACTCCTACACAGTCAGCAACAAGCCTCCCATCAAAGAGATCGCCCTCGCCCTCGCCCTCCTCGTCACCGGCACTCTCGGTCTTGTAATCGGCATCCTCATGGCCTACAACCATGTCGGCGGCGACAAAGCCCACGGGTTATTTTTTGCGATACTTGGTGGGATCTTGTTTCTTCCGGGCTTCTATTACACGCGGATTGCTTACTATGCGTATAAAGGGTACAAGGGCTTCTCCTTCTCCAACATTCCACCGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF872

Protein Analysis

104

Amino Acids

11.31

Weight (kDa)

5.79

Isoelectric Point (pI)

34.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM_230_134 PF05915 26 - 101 1.3e-17 Transmembrane proteins 230/134
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 20, 247
AciI CCGC 2 cut(s) 174, 247
AclWI GGATC 1 cut(s) 224
AfaI GTAC 2 cut(s) 8, 275
AflIII ACRYGT 1 cut(s) 18
AgeI ACCGGT 1 cut(s) 304
AlwI GGATC 1 cut(s) 224
AoxI GGCC 1 cut(s) 156
AsiGI ACCGGT 1 cut(s) 304
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 112
BarI GAAGNNNNNNTAC 2 cut(s) 266, 298
BccI CCATC 2 cut(s) 43, 92
BcnI CCSGG 1 cut(s) 231
BisI GCNGC 1 cut(s) 175
BlsI GCNGC 1 cut(s) 176
Bme1390I CCNGG 1 cut(s) 231
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 187
BsaWI WCCGGW 1 cut(s) 304
Bse118I RCCGGY 2 cut(s) 122, 304
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 104
Bsh1236I CGCG 2 cut(s) 20, 247
BshFI GGCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 304
BsiSI CCGG 3 cut(s) 123, 230, 305
BsiWI CGTACG 1 cut(s) 6
BsnI GGCC 1 cut(s) 158
Bsp143I GATC 3 cut(s) 13, 93, 216
BspACI CCGC 2 cut(s) 174, 247
BspANI GGCC 1 cut(s) 158
BspFNI CGCG 2 cut(s) 20, 247
BspPI GGATC 1 cut(s) 224
BsrFI RCCGGY 2 cut(s) 122, 304
BssAI RCCGGY 2 cut(s) 122, 304
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 3 cut(s) 13, 93, 216
Bst4CI ACNGT 1 cut(s) 67
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 2 cut(s) 20, 247
BstKTI GATC 3 cut(s) 16, 96, 219
BstMBI GATC 3 cut(s) 13, 93, 216
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstSCI CCNGG 1 cut(s) 229
BstUI CGCG 2 cut(s) 20, 247
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 1 cut(s) 158
BtgI CCRYGG 1 cut(s) 187
BtsCI GGATG 1 cut(s) 147
Cfr10I RCCGGY 2 cut(s) 122, 304
Csp6I GTAC 2 cut(s) 7, 274
CspAI ACCGGT 1 cut(s) 304
CviAII CATG 3 cut(s) 43, 154, 167
CviJI RGCY 5 cut(s) 79, 158, 185, 234, 282
CviKI_1 RGCY 5 cut(s) 79, 158, 185, 234, 282
CviQI GTAC 2 cut(s) 7, 274
DpnI GATC 3 cut(s) 15, 95, 218
DpnII GATC 3 cut(s) 13, 93, 216
FaeI CATG 3 cut(s) 46, 157, 170
FaiI YATR 5 cut(s) 44, 155, 168, 261, 267
FatI CATG 3 cut(s) 42, 153, 166
Fnu4HI GCNGC 1 cut(s) 175
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 1 cut(s) 175
GluI GCNGC 1 cut(s) 175
HaeIII GGCC 1 cut(s) 158
HapII CCGG 3 cut(s) 123, 230, 305
Hin1II CATG 3 cut(s) 46, 157, 170
HinfI GANTC 1 cut(s) 56
HpaII CCGG 3 cut(s) 123, 230, 305
HphI GGTGA 1 cut(s) 112
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 298
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 9
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 3 cut(s) 46, 157, 170
Kzo9I GATC 3 cut(s) 13, 93, 216
LpnPI CCDG 2 cut(s) 136, 243
LweI GCATC 1 cut(s) 156
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 1 cut(s) 118
MalI GATC 3 cut(s) 15, 95, 218
MboI GATC 3 cut(s) 13, 93, 216
MboII GAAGA 1 cut(s) 218
MflI RGATCY 1 cut(s) 216
MluI ACGCGT 1 cut(s) 18
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 8 cut(s) 31, 46, 90, 110, 116, 122, 125, 161
MslI CAYNNNNRTG 2 cut(s) 47, 152
MspI CCGG 3 cut(s) 123, 230, 305
MspR9I CCNGG 1 cut(s) 231
MvnI CGCG 2 cut(s) 20, 247
MwoI GCNNNNNNNGC 1 cut(s) 260
NciI CCSGG 1 cut(s) 231
NdeII GATC 3 cut(s) 13, 93, 216
NlaIII CATG 3 cut(s) 46, 157, 170
NmuCI GTSAC 1 cut(s) 118
PcsI WCGNNNNNNNCGW 1 cut(s) 114
Pfl23II CGTACG 1 cut(s) 6
PinAI ACCGGT 1 cut(s) 304
PkrI GCNGC 1 cut(s) 176
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PspLI CGTACG 1 cut(s) 6
PsuI RGATCY 1 cut(s) 216
RsaI GTAC 2 cut(s) 8, 275
RsaNI GTAC 2 cut(s) 7, 274
RseI CAYNNNNRTG 2 cut(s) 47, 152
SatI GCNGC 1 cut(s) 175
Sau3AI GATC 3 cut(s) 13, 93, 216
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 1 cut(s) 231
SetI ASST 1 cut(s) 12
SfaNI GCATC 1 cut(s) 156
SgrAI CRCCGGYG 1 cut(s) 304
SmiMI CAYNNNNRTG 2 cut(s) 47, 152
SsiI CCGC 2 cut(s) 174, 247
StyD4I CCNGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 12
TaqI TCGA 1 cut(s) 12
TaqII GACCGA 1 cut(s) 122
TauI GCSGC 1 cut(s) 177
TseFI GTSAC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.