AT4G31040

Chloroplast envelope membrane protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004997
Physical Location & Seq
Reverse (-)
15111477 .. 15114126
2650 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31040.1

Sequence Viewer

Length: 1317 bp
ATGTCCTTGATGAGCTCGTCAATGGTCTTATGCCACTGCTTATCATTCAGTAGTCAAAACCCAGATCCTGAATCATCATCATCTTCTCTTCTTCGTTATAAGCCTTGTGATTCGATTTCGTTGTGGGGTAAGAGAAGGAAGAAGCTTTGGAGATTTGTTCCCAGTGCGGAGAAGAATAACAGTCATACGGGTAATAATAATAAGAGGAGAAGAAGCTGGTGGCAGAGGTTTTTCTTTGATGATGATGGGAATTGGCTTGGTTTGAGAGACGACGACATTGTTGATGAGACTACTGAGCTTGCAAAGGATGATGAGATGTCTGATGAAGAGAAATTCGAGACTTGGAAGAGACGAGCAGAGGCGATTGTCGAGCTACGGGAAGGTCAGGAGGAGATTGGGGATAGTGGTGTTGTTGCTGATGTGACCAAGAAATGGGAGGATTGGATTGTGGATTCAGATGATAGTTTTGTTGAGTCTTGGACCCGAGACTCTGCTGGTAGTGACGACAATTTAGAGCTTGATGAATTGACAATTCCAGATGGAGGGTTGGTTAAAATGGTGAGGGATATGGTTTTAGGTGCAGAAGAGGAAGATATTCTTTACGAAGACCGTATTTTTCGTTACGCTTCTTCAAAATCGGCAAAGTTTTTGGCGGTCTTAATTCTCATTCCATGGGCATTAGACTTTTTGGCTCATGATTATCTACTTATGCCCTTCTTAGACAGATATGTGAAGACTGTACCACTTGCTGCCCAGACGCTTGATGTGAGACGAAATCAGAAGCTAGAAATGGTTAAGGAACTTAATAGAGAAAAAGCTAGGTACCGTCTTGAAGTTGAGATTGGCAAATCTCCGCCGCTTTCTGACGATGATTTATGGTGGGAGATGCGGGGTAAAGCTTTGGAACTGCGTGATGAGTGGAGACTAGAGAACCGGAAAGCATTTGCCAACATATGGTCTGATATGGTGTTTGGGATCTCCTTGTTTGTTCTTTTGTACGCCAATCAGGGTAGAGTAGCCTTGTTGAAATTTACAGGATATAAAATCATAAACAACATTTCAGACACGGGAAAGGCATTTCTCATCATCCTGATCACCGACATATTCTTGGGGTACCATTCTGAATCTGGATGGGAGACATTGTTAGAGATAATAATGGAACATTATGGTCTTGAAGTTGAGCAATCTACTATTACCATTTTTATCTGCCTAGTTCCCGTTATTATGGACGCTTGTGTTAAGCTCTGGCTGTTTAAGTTCCTTCCAAGACTGTCTCCGCGAGTTTCCAACATATTCCAGGAGATGAAACGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

438

Amino Acids

51.02

Weight (kDa)

4.98

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 207 - 436 3.9e-78 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 99
Acc65I GGTACC 2 cut(s) 822, 1113
AccB1I GGYRCC 2 cut(s) 822, 1113
AccB7I CCANNNNNTGG 2 cut(s) 432, 954
AccII CGCG 1 cut(s) 1279
AciI CCGC 6 cut(s) 167, 653, 854, 857, 889, 1277
AclWI GGATC 2 cut(s) 59, 983
AcsI RAATTY 2 cut(s) 332, 1028
AfaI GTAC 4 cut(s) 741, 824, 998, 1115
AfiI CCNNNNNNNGG 3 cut(s) 432, 542, 954
AgsI TTSAA 4 cut(s) 633, 833, 1027, 1175
AjnI CCWGG 1 cut(s) 1296
AjuI GAANNNNNNNTTGG 2 cut(s) 825, 857
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 9 cut(s) 261, 281, 332, 343, 480, 763, 916, 1130, 1278
AlwI GGATC 2 cut(s) 59, 983
AlwNI CAGNNNCTG 1 cut(s) 68
Ama87I CYCGRG 1 cut(s) 483
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 2 cut(s) 332, 1028
Asp700I GAANNNNTTC 1 cut(s) 594
Asp718I GGTACC 2 cut(s) 822, 1113
AspS9I GGNCC 1 cut(s) 480
AsuHPI GGTGA 2 cut(s) 571, 1087
AvaI CYCGRG 1 cut(s) 483
AvaII GGWCC 1 cut(s) 480
BanI GGYRCC 2 cut(s) 822, 1113
BanII GRGCYC 1 cut(s) 17
BbsI GAAGAC 2 cut(s) 612, 740
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 736
BccI CCATC 3 cut(s) 239, 533, 1125
BciT130I CCWGG 1 cut(s) 1298
BclI TGATCA 1 cut(s) 1092
BcoDI GTCTC 9 cut(s) 261, 281, 332, 343, 480, 763, 916, 1130, 1278
BfaI CTAG 4 cut(s) 785, 819, 926, 1211
BisI GCNGC 2 cut(s) 750, 857
BlsI GCNGC 2 cut(s) 751, 858
Bme1390I CCNGG 1 cut(s) 1298
Bme18I GGWCC 1 cut(s) 480
BmeT110I CYCGRG 1 cut(s) 483
BmgT120I GGNCC 1 cut(s) 480
BmiI GGNNCC 3 cut(s) 482, 824, 1115
BmrFI CCNGG 1 cut(s) 1298
BmrI ACTGGG 1 cut(s) 156
BmsI GCATC 1 cut(s) 876
BmuI ACTGGG 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 612, 740
BsaJI CCNNGG 1 cut(s) 671
BsaWI WCCGGW 1 cut(s) 933
BsaXI ACNNNNNCTCC 4 cut(s) 534, 564, 1127, 1157
Bsc4I CCNNNNNNNGG 3 cut(s) 432, 542, 954
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 1298
BseDI CCNNGG 1 cut(s) 671
BseGI GGATG 3 cut(s) 313, 1086, 1136
BseLI CCNNNNNNNGG 3 cut(s) 432, 542, 954
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 2 cut(s) 220, 404
BseXI GCAGC 1 cut(s) 736
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 1279
BshNI GGYRCC 2 cut(s) 822, 1113
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 1 cut(s) 934
BslI CCNNNNNNNGG 3 cut(s) 432, 542, 954
BsmAI GTCTC 9 cut(s) 261, 281, 332, 343, 480, 763, 916, 1130, 1278
BsmBI CGTCTC 3 cut(s) 261, 343, 763
BsoBI CYCGRG 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 3 cut(s) 64, 975, 1092
Bsp19I CCATGG 1 cut(s) 671
BspACI CCGC 6 cut(s) 167, 653, 854, 857, 889, 1277
BspCNI CTCAG 1 cut(s) 286
BspFNI CGCG 1 cut(s) 1279
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 3 cut(s) 482, 824, 1115
BspPI GGATC 2 cut(s) 59, 983
BspT107I GGYRCC 2 cut(s) 822, 1113
BsrI ACTGG 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 671
BssMI GATC 3 cut(s) 64, 975, 1092
BssT1I CCWWGG 1 cut(s) 671
Bst2UI CCWGG 1 cut(s) 1298
Bst4CI ACNGT 5 cut(s) 182, 611, 739, 827, 1272
Bst6I CTCTTC 4 cut(s) 93, 321, 341, 579
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 2 cut(s) 294, 718
BstDSI CCRYGG 1 cut(s) 671
BstF5I GGATG 3 cut(s) 313, 1086, 1136
BstFNI CGCG 1 cut(s) 1279
BstKTI GATC 3 cut(s) 67, 978, 1095
BstMAI GTCTC 9 cut(s) 261, 281, 332, 343, 480, 763, 916, 1130, 1278
BstMBI GATC 3 cut(s) 64, 975, 1092
BstNI CCWGG 1 cut(s) 1298
BstSCI CCNGG 1 cut(s) 1296
BstUI CGCG 1 cut(s) 1279
BstV1I GCAGC 1 cut(s) 736
BstV2I GAAGAC 2 cut(s) 612, 740
BstX2I RGATCY 2 cut(s) 64, 975
BstYI RGATCY 2 cut(s) 64, 975
BtgI CCRYGG 1 cut(s) 671
BtsCI GGATG 3 cut(s) 313, 1086, 1136
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 3 cut(s) 34, 169, 1312
Cac8I GCNNGC 1 cut(s) 300
CaiI CAGNNNCTG 1 cut(s) 68
CciI TCATGA 1 cut(s) 694
Cfr13I GGNCC 1 cut(s) 480
CseI GACGC 2 cut(s) 766, 1238
Csp6I GTAC 4 cut(s) 740, 823, 997, 1114
CviAII CATG 2 cut(s) 672, 695
CviQI GTAC 4 cut(s) 740, 823, 997, 1114
DdeI CTNAG 2 cut(s) 294, 718
DpnI GATC 3 cut(s) 66, 977, 1094
DpnII GATC 3 cut(s) 64, 975, 1092
Eam1104I CTCTTC 4 cut(s) 93, 321, 341, 579
EarI CTCTTC 4 cut(s) 93, 321, 341, 579
EciI GGCGGA 1 cut(s) 843
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 671
Eco24I GRGCYC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 480
Eco53kI GAGCTC 1 cut(s) 15
Eco88I CYCGRG 1 cut(s) 483
EcoICRI GAGCTC 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 1296
EcoT14I CCWWGG 1 cut(s) 671
EcoT38I GRGCYC 1 cut(s) 17
ErhI CCWWGG 1 cut(s) 671
Esp3I CGTCTC 3 cut(s) 261, 343, 763
FaeI CATG 2 cut(s) 675, 698
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FatI CATG 2 cut(s) 671, 694
FauI CCCGC 1 cut(s) 882
FauNDI CATATG 1 cut(s) 953
FbaI TGATCA 1 cut(s) 1092
Fnu4HI GCNGC 2 cut(s) 750, 857
FokI GGATG 3 cut(s) 320, 1073, 1143
FriOI GRGCYC 1 cut(s) 17
Fsp4HI GCNGC 2 cut(s) 750, 857
FspBI CTAG 4 cut(s) 785, 819, 926, 1211
GluI GCNGC 2 cut(s) 750, 857
HapII CCGG 1 cut(s) 934
HgaI GACGC 2 cut(s) 766, 1238
Hin1II CATG 2 cut(s) 675, 698
HindIII AAGCTT 2 cut(s) 143, 897
HinfI GANTC 6 cut(s) 71, 110, 452, 473, 488, 1124
HpaII CCGG 1 cut(s) 934
HphI GGTGA 2 cut(s) 571, 1087
Hpy188I TCNGA 7 cut(s) 322, 457, 780, 865, 961, 1063, 1123
Hpy188III TCNNGA 9 cut(s) 68, 337, 386, 536, 695, 830, 1090, 1128, 1172
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 4 cut(s) 129, 374, 724, 1271
HpyCH4III ACNGT 5 cut(s) 182, 611, 739, 827, 1272
HpyCH4IV ACGT 1 cut(s) 1309
HpyCH4V TGCA 2 cut(s) 302, 581
HpyF3I CTNAG 2 cut(s) 294, 718
HpySE526I ACGT 1 cut(s) 1309
Hsp92II CATG 2 cut(s) 675, 698
KpnI GGTACC 2 cut(s) 826, 1117
Ksp22I TGATCA 1 cut(s) 1092
Kzo9I GATC 3 cut(s) 64, 975, 1092
Lsp1109I GCAGC 1 cut(s) 736
LweI GCATC 1 cut(s) 876
MaeI CTAG 4 cut(s) 785, 819, 926, 1211
MaeII ACGT 1 cut(s) 1309
MaeIII GTNAC 4 cut(s) 421, 500, 620, 1310
MalI GATC 3 cut(s) 66, 977, 1094
MboI GATC 3 cut(s) 64, 975, 1092
MflI RGATCY 2 cut(s) 64, 975
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 7 cut(s) 250, 332, 508, 524, 531, 660, 1028
MlyI GAGTC 2 cut(s) 482, 482
MmeI TCCRAC 1 cut(s) 1311
MnlI CCTC 8 cut(s) 198, 219, 352, 382, 430, 536, 555, 580
MroXI GAANNNNTTC 1 cut(s) 594
MseI TTAA 6 cut(s) 552, 659, 795, 804, 1239, 1254
MspI CCGG 1 cut(s) 934
MspR9I CCNGG 1 cut(s) 1298
MvaI CCWGG 1 cut(s) 1298
MvnI CGCG 1 cut(s) 1279
NcoI CCATGG 1 cut(s) 671
NdeI CATATG 1 cut(s) 953
NdeII GATC 3 cut(s) 64, 975, 1092
NlaIII CATG 2 cut(s) 675, 698
NlaIV GGNNCC 3 cut(s) 482, 824, 1115
NmuCI GTSAC 3 cut(s) 421, 500, 1310
PagI TCATGA 1 cut(s) 694
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 4 cut(s) 71, 110, 452, 1124
PflMI CCANNNNNTGG 2 cut(s) 432, 954
PfoI TCCNGGA 1 cut(s) 1296
PkrI GCNGC 2 cut(s) 751, 858
PleI GAGTC 2 cut(s) 481, 482
PpsI GAGTC 2 cut(s) 481, 482
PsiI TTATAA 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 1296
PspGI CCWGG 1 cut(s) 1296
PspN4I GGNNCC 3 cut(s) 482, 824, 1115
PspPI GGNCC 1 cut(s) 480
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 2 cut(s) 64, 975
RsaI GTAC 4 cut(s) 741, 824, 998, 1115
RsaNI GTAC 4 cut(s) 740, 823, 997, 1114
SacI GAGCTC 1 cut(s) 17
SaqAI TTAA 6 cut(s) 552, 659, 795, 804, 1239, 1254
SatI GCNGC 2 cut(s) 750, 857
Sau3AI GATC 3 cut(s) 64, 975, 1092
Sau96I GGNCC 1 cut(s) 480
SchI GAGTC 2 cut(s) 482, 482
ScrFI CCNGG 1 cut(s) 1298
SduI GDGCHC 1 cut(s) 17
SfaNI GCATC 1 cut(s) 876
SinI GGWCC 1 cut(s) 480
Sse9I AATT 7 cut(s) 250, 332, 508, 524, 531, 660, 1028
SsiI CCGC 6 cut(s) 167, 653, 854, 857, 889, 1277
SspMI CTAG 4 cut(s) 785, 819, 926, 1211
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 1 cut(s) 1296
StyI CCWWGG 1 cut(s) 671
TaaI ACNGT 5 cut(s) 182, 611, 739, 827, 1272
TaiI ACGT 1 cut(s) 1312
TaqI TCGA 3 cut(s) 113, 336, 369
TasI AATT 7 cut(s) 250, 332, 508, 524, 531, 660, 1028
TauI GCSGC 1 cut(s) 859
TfiI GAWTC 4 cut(s) 71, 110, 452, 1124
Tru1I TTAA 6 cut(s) 552, 659, 795, 804, 1239, 1254
Tru9I TTAA 6 cut(s) 552, 659, 795, 804, 1239, 1254
TscAI CASTG 2 cut(s) 41, 169
TseFI GTSAC 3 cut(s) 421, 500, 1310
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 3 cut(s) 421, 500, 1310
TspDTI ATGAA 2 cut(s) 339, 537
TspRI CASTG 2 cut(s) 41, 169
Van91I CCANNNNNTGG 2 cut(s) 432, 954
VpaK11BI GGWCC 1 cut(s) 480
XapI RAATTY 2 cut(s) 332, 1028
XcmI CCANNNNNNNNNTGG 1 cut(s) 1124
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 4 cut(s) 785, 819, 926, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.