pycom420g00290

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0004997
Physical Location & Seq
Forward (+)
371185 .. 374389
3205 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00290.1

Sequence Viewer

Length: 1053 bp
ATGTTAATCATGTCCTCCTCCGCCGTCTTGTGCAAAAGCTCGATCTTTGTTAACTCCAAGTTCAGTCTGCTCAGCCGCAACTCCATTTTCCACAGAAATTTGTCGACGCCACTGCCTCTTGTGGTTGATACAAGGCGGCGGAGGTCTGCCGCTGCAGAGCACATTCCAAATGCTTCTGGAAAAAGGAAAAGGACTTGGTGGCAAACATTCTTTTTCGACGACGACGGGAATTGGCTCGGTTTGAAGGAGGACGACATGCTGGCGGCGGAGGAGGAGCAAGAAGAGGAGTTAGATGGCTCCTCCCCAGCTGCCGCCAACAGCAGAGGCGAAGAAGCTGAGAAATTTGAGGCGTGGAAGACGAGGGCAGAAGCAATCGTGGAGTTGAGGGAAGCACAGCAGGATATGACCAACGAGGAGAACCGCATGTGGGAGGATTGGATTTCGGATTCTAATTCTAACTCTAATTCTTCTACTTCCTCCTGGGAAGCATCTGTGCAGTCGGATCCTACTCAAGAAAGGGGATTGATTTACTCTCTCAGCGATTTTGTCCTCGGAAAGGAAGAAGATGATGAGGAGGACATGCTCTATGAAGACCGCGTTTTTCGCTATGCCTCACTCAATTCGGCCAAATTTTTGGCAGTATTAGTTATCATACCCTGGGCCTTGGATTTTGTGGTTCACGACTATGTTCTGATGCCTTTCTTAGACAGATATGTGAAGACTGTACCACTTGCAGCACAGTTGCTTGATGTCAGACGAAATCAAAAGCTTCAAATGGTTGAGGAATTAAAAATGGAGGGAGCAAGATTTCATCTTGAGATGGAAATTGGAAAATCTCCACCGCTTTCTGATGATGAGGTTTGGTGGGAGTTGCGGCATAAAGCTTATAGCCGGACATTTGTCTCAGTGCACTGCACACTTGGACTTTTGGATCTACATAACAAAAAATACTCTCATTTCAGAGATCCAAATGCCCTAGTATGCAATGAGAAAAATGTCCAGGTTATAATTGTAAAAGGTCATCAAGTATTCTCACTTTTTAATTATGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

351

Amino Acids

40.37

Weight (kDa)

5.04

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 202 - 296 3.2e-15 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1007
AccI GTMKAC 1 cut(s) 104
AccII CGCG 1 cut(s) 597
AclWI GGATC 4 cut(s) 497, 510, 939, 959
AcoI YGGCCR 1 cut(s) 624
AcsI RAATTY 3 cut(s) 97, 341, 629
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 1 cut(s) 726
AfiI CCNNNNNNNGG 2 cut(s) 427, 556
AgsI TTSAA 2 cut(s) 244, 773
AjnI CCWGG 3 cut(s) 479, 656, 999
AluBI AGCT 5 cut(s) 39, 308, 335, 769, 884
AluI AGCT 5 cut(s) 39, 308, 335, 769, 884
Alw21I GWGCWC 2 cut(s) 162, 912
Alw26I GTCTC 1 cut(s) 907
Alw44I GTGCAC 1 cut(s) 908
AlwI GGATC 4 cut(s) 497, 510, 939, 959
AoxI GGCC 2 cut(s) 624, 660
ApaLI GTGCAC 1 cut(s) 908
ApeKI GCWGC 3 cut(s) 152, 308, 734
ApoI RAATTY 3 cut(s) 97, 341, 629
AspS9I GGNCC 1 cut(s) 660
BaeGI GKGCMC 1 cut(s) 912
BamHI GGATCC 1 cut(s) 502
BbsI GAAGAC 3 cut(s) 362, 597, 725
Bbv12I GWGCWC 2 cut(s) 162, 912
BbvI GCAGC 3 cut(s) 139, 295, 746
BccI CCATC 2 cut(s) 287, 814
BceAI ACGGC 1 cut(s) 8
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 3 cut(s) 481, 658, 1001
BcoDI GTCTC 1 cut(s) 907
BfaI CTAG 1 cut(s) 977
BfmI CTRYAG 1 cut(s) 153
BisI GCNGC 9 cut(s) 76, 137, 150, 153, 264, 309, 312, 735, 875
BlpI GCTNAGC 1 cut(s) 71
BlsI GCNGC 9 cut(s) 77, 138, 151, 154, 265, 310, 313, 736, 876
Bme1390I CCNGG 3 cut(s) 481, 658, 1001
BmgT120I GGNCC 1 cut(s) 660
BmiI GGNNCC 2 cut(s) 298, 504
BmrFI CCNGG 3 cut(s) 481, 658, 1001
BmsI GCATC 2 cut(s) 497, 684
BoxI GACNNNNGTC 1 cut(s) 899
BpiI GAAGAC 3 cut(s) 362, 597, 725
Bpu1102I GCTNAGC 1 cut(s) 71
BpuEI CTTGAG 2 cut(s) 495, 836
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 5 cut(s) 480, 550, 656, 657, 663
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 556
Bse3DI GCAATG 1 cut(s) 991
BseBI CCWGG 3 cut(s) 481, 658, 1001
BseDI CCNNGG 5 cut(s) 480, 550, 656, 657, 663
BseLI CCNNNNNNNGG 2 cut(s) 427, 556
BseMI GCAATG 1 cut(s) 991
BseMII CTCAG 4 cut(s) 85, 327, 550, 918
BseRI GAGGAG 7 cut(s) 7, 284, 287, 289, 299, 428, 587
BseSI GKGCMC 1 cut(s) 912
BseXI GCAGC 3 cut(s) 139, 295, 746
BseYI CCCAGC 1 cut(s) 304
BsgI GTGCAG 2 cut(s) 515, 898
Bsh1236I CGCG 1 cut(s) 597
BshFI GGCC 2 cut(s) 626, 662
BsiHKAI GWGCWC 2 cut(s) 162, 912
BsiSI CCGG 1 cut(s) 892
BslI CCNNNNNNNGG 2 cut(s) 427, 556
BsmAI GTCTC 1 cut(s) 907
BsnI GGCC 2 cut(s) 626, 662
Bsp1286I GDGCHC 2 cut(s) 162, 912
Bsp143I GATC 4 cut(s) 42, 502, 931, 964
Bsp1720I GCTNAGC 1 cut(s) 71
BspANI GGCC 2 cut(s) 626, 662
BspCNI CTCAG 4 cut(s) 84, 328, 549, 917
BspFNI CGCG 1 cut(s) 597
BspLI GGNNCC 2 cut(s) 298, 504
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 4 cut(s) 497, 510, 939, 959
BsrDI GCAATG 1 cut(s) 991
BssECI CCNNGG 5 cut(s) 480, 550, 656, 657, 663
BssMI GATC 4 cut(s) 42, 502, 931, 964
BssNI GRCGYC 1 cut(s) 107
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 3 cut(s) 481, 658, 1001
Bst4CI ACNGT 2 cut(s) 724, 741
Bst6I CTCTTC 1 cut(s) 276
BstACI GRCGYC 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 5 cut(s) 71, 336, 536, 703, 904
BstENI CCTNNNNNAGG 1 cut(s) 554
BstFNI CGCG 1 cut(s) 597
BstKTI GATC 4 cut(s) 45, 505, 934, 967
BstMAI GTCTC 1 cut(s) 907
BstMBI GATC 4 cut(s) 42, 502, 931, 964
BstMWI GCNNNNNNNGC 1 cut(s) 603
BstNI CCWGG 3 cut(s) 481, 658, 1001
BstNSI RCATGY 3 cut(s) 259, 427, 583
BstPAI GACNNNNGTC 1 cut(s) 899
BstSCI CCNGG 3 cut(s) 479, 656, 999
BstSFI CTRYAG 1 cut(s) 153
BstSLI GKGCMC 1 cut(s) 912
BstUI CGCG 1 cut(s) 597
BstV1I GCAGC 3 cut(s) 139, 295, 746
BstV2I GAAGAC 3 cut(s) 362, 597, 725
BstX2I RGATCY 3 cut(s) 502, 931, 964
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 3 cut(s) 502, 931, 964
BsuRI GGCC 2 cut(s) 626, 662
BtsI GCAGTG 2 cut(s) 110, 910
BtsIMutI CAGTG 3 cut(s) 110, 910, 912
Cac8I GCNNGC 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 660
CseI GACGC 1 cut(s) 115
Csp6I GTAC 1 cut(s) 725
CviAII CATG 4 cut(s) 10, 256, 424, 580
CviQI GTAC 1 cut(s) 725
DdeI CTNAG 5 cut(s) 71, 336, 536, 703, 904
DpnI GATC 4 cut(s) 44, 504, 933, 966
DpnII GATC 4 cut(s) 42, 502, 931, 964
EaeI YGGCCR 1 cut(s) 624
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
EciI GGCGGA 3 cut(s) 10, 154, 281
Eco130I CCWWGG 1 cut(s) 663
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoRII CCWGG 3 cut(s) 479, 656, 999
EcoT14I CCWWGG 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 663
FaeI CATG 4 cut(s) 13, 259, 427, 583
FatI CATG 4 cut(s) 9, 255, 423, 579
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 9 cut(s) 76, 137, 150, 153, 264, 309, 312, 735, 875
Fsp4HI GCNGC 9 cut(s) 76, 137, 150, 153, 264, 309, 312, 735, 875
FspBI CTAG 1 cut(s) 977
GluI GCNGC 9 cut(s) 76, 137, 150, 153, 264, 309, 312, 735, 875
GsaI CCCAGC 1 cut(s) 308
HaeIII GGCC 2 cut(s) 626, 662
HapII CCGG 1 cut(s) 892
HgaI GACGC 1 cut(s) 115
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 4 cut(s) 13, 259, 427, 583
HincII GTYRAC 2 cut(s) 52, 105
HindII GTYRAC 2 cut(s) 52, 105
HindIII AAGCTT 2 cut(s) 767, 882
HinfI GANTC 1 cut(s) 446
HpaI GTTAAC 1 cut(s) 52
HpaII CCGG 1 cut(s) 892
Hpy166II GTNNAC 4 cut(s) 52, 105, 679, 910
Hpy188I TCNGA 7 cut(s) 445, 502, 554, 693, 755, 850, 962
Hpy188III TCNNGA 4 cut(s) 177, 512, 680, 815
Hpy8I GTNNAC 4 cut(s) 52, 105, 679, 910
Hpy99I CGWCG 4 cut(s) 109, 221, 224, 227
HpyAV CCTTC 1 cut(s) 238
HpyCH4III ACNGT 2 cut(s) 724, 741
HpyCH4V TGCA 7 cut(s) 33, 155, 496, 734, 910, 915, 984
HpyF10VI GCNNNNNNNGC 1 cut(s) 603
HpyF3I CTNAG 5 cut(s) 71, 336, 536, 703, 904
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 4 cut(s) 13, 259, 427, 583
KspAI GTTAAC 1 cut(s) 52
Kzo9I GATC 4 cut(s) 42, 502, 931, 964
LmnI GCTCC 3 cut(s) 274, 302, 800
Lsp1109I GCAGC 3 cut(s) 139, 295, 746
LweI GCATC 2 cut(s) 497, 684
MaeI CTAG 1 cut(s) 977
MalI GATC 4 cut(s) 44, 504, 933, 966
MboI GATC 4 cut(s) 42, 502, 931, 964
MboII GAAGA 8 cut(s) 293, 341, 367, 459, 572, 575, 602, 730
MflI RGATCY 3 cut(s) 502, 931, 964
MhlI GDGCHC 2 cut(s) 162, 912
MmeI TCCRAC 1 cut(s) 480
MseI TTAA 4 cut(s) 5, 51, 788, 1041
MslI CAYNNNNRTG 1 cut(s) 684
MspA1I CMGCKG 2 cut(s) 152, 308
MspI CCGG 1 cut(s) 892
MspR9I CCNGG 3 cut(s) 481, 658, 1001
MvaI CCWGG 3 cut(s) 481, 658, 1001
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 1 cut(s) 603
NdeII GATC 4 cut(s) 42, 502, 931, 964
NlaIII CATG 4 cut(s) 13, 259, 427, 583
NlaIV GGNNCC 2 cut(s) 298, 504
NspI RCATGY 3 cut(s) 259, 427, 583
PasI CCCWGGG 1 cut(s) 657
PfeI GAWTC 1 cut(s) 446
PkrI GCNGC 9 cut(s) 77, 138, 151, 154, 265, 310, 313, 736, 876
PshAI GACNNNNGTC 1 cut(s) 899
PsiI TTATAA 1 cut(s) 1007
Psp6I CCWGG 3 cut(s) 479, 656, 999
PspFI CCCAGC 1 cut(s) 304
PspGI CCWGG 3 cut(s) 479, 656, 999
PspN4I GGNNCC 2 cut(s) 298, 504
PspPI GGNCC 1 cut(s) 660
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 3 cut(s) 502, 931, 964
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 1 cut(s) 726
RsaNI GTAC 1 cut(s) 725
RseI CAYNNNNRTG 1 cut(s) 684
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 4 cut(s) 5, 51, 788, 1041
SatI GCNGC 9 cut(s) 76, 137, 150, 153, 264, 309, 312, 735, 875
Sau3AI GATC 4 cut(s) 42, 502, 931, 964
Sau96I GGNCC 1 cut(s) 660
ScrFI CCNGG 3 cut(s) 481, 658, 1001
SduI GDGCHC 2 cut(s) 162, 912
SetI ASST 9 cut(s) 41, 146, 310, 337, 771, 861, 886, 1005, 1021
SfaNI GCATC 2 cut(s) 497, 684
SfcI CTRYAG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 684
SmlI CTYRAG 2 cut(s) 510, 815
SmoI CTYRAG 2 cut(s) 510, 815
SspMI CTAG 1 cut(s) 977
StyD4I CCNGG 3 cut(s) 479, 656, 999
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 2 cut(s) 724, 741
TaqI TCGA 3 cut(s) 41, 104, 216
TauI GCSGC 6 cut(s) 78, 139, 152, 266, 314, 877
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 4 cut(s) 5, 51, 788, 1041
Tru9I TTAA 4 cut(s) 5, 51, 788, 1041
TscAI CASTG 3 cut(s) 117, 912, 917
TseI GCWGC 3 cut(s) 152, 308, 734
TspDTI ATGAA 2 cut(s) 603, 800
TspRI CASTG 3 cut(s) 117, 912, 917
VneI GTGCAC 1 cut(s) 908
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 3 cut(s) 97, 341, 629
XceI RCATGY 3 cut(s) 259, 427, 583
XmiI GTMKAC 1 cut(s) 104
XspI CTAG 1 cut(s) 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.