RchiOBHm_Chr4g0384781

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004997
Physical Location & Seq
Forward (+)
130122 .. 136377
6256 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35833

Sequence Viewer

Length: 1248 bp
ATGTTGTCCATGGTCTTATGCGAGAACTTCATCTGTTTTAACAACCACAACAAGCAGACTTTGTTTTCGCACAAAATTGATTCGCAGTCGAGGCTCGCAGCAGCAGTTGAGGTGGGTCGAGGAGGAGGAGGACGGAGGTGCAGTGGGTTCGTTGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCGGAGGAGGAGGACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGGGCCGAAGCAATCGTGGATTTGAGGGAAGCACAGCAGGATATGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCCAACTCGTCCTCGTCCTGGGAATACATGCAGTCTGATATTCCTACCGAAACTGAAGCCGAAAAGGGGGTGCTTTACTCTCTCACCGATTCCCTTCTTGGAAGGGAAGATGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCCCTCAATTCGGCTAAATTTTTGGCGGTTTTAATAATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCGGCAGAGATGCTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATGGAGAGAGCAAGATTTCAGCTTGAGATGGAAATTGGTAAAGCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTCATTTGGGATCTCATTATTCATTCTTTTATACTGCAACCAGAGTAAAGTAGCTTTGCTGAAATTTACAGGTTATAAGATAATAAATAATATTTCAGATACTGGGAAGGCATTTCTAATTATACTCATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAGACTTTGATAGAAATTTTTGTCGAGCATTATGGGCTTCAGGTTGATCAAGCAGTTATAACCATTTTTATCTGCTTGATTCCAGTTATTATTGACGCATGCGTGAAACTTTGGCTGTTTAAGTTTCTTCCAAGATTATCCCCGAAGGTGGCAAACATATTCCGGGAGATGCAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

415

Amino Acids

48.25

Weight (kDa)

4.97

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 184 - 413 3e-76 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 972, 1124
Acc36I ACCTGC 1 cut(s) 534
AccB7I CCANNNNNTGG 1 cut(s) 885
AccII CGCG 1 cut(s) 251
AciI CCGC 6 cut(s) 249, 251, 401, 584, 680, 820
AclWI GGATC 1 cut(s) 914
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 4 cut(s) 296, 575, 959, 1080
AcuI CTGAAG 2 cut(s) 480, 1088
AfiI CCNNNNNNNGG 5 cut(s) 423, 471, 568, 885, 1213
AgsI TTSAA 3 cut(s) 229, 719, 860
AjnI CCWGG 1 cut(s) 422
AluBI AGCT 7 cut(s) 192, 290, 715, 760, 782, 838, 950
AluI AGCT 7 cut(s) 192, 290, 715, 760, 782, 838, 950
AlwI GGATC 1 cut(s) 914
AlwNI CAGNNNCTG 1 cut(s) 998
AoxI GGCC 2 cut(s) 246, 318
ApeKI GCWGC 4 cut(s) 98, 101, 398, 1237
ApoI RAATTY 4 cut(s) 296, 575, 959, 1080
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
AspLEI GCGC 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 318, 888
AsuC2I CCSGG 1 cut(s) 1229
AsuHPI GGTGA 1 cut(s) 481
AvaII GGWCC 1 cut(s) 888
BbsI GAAGAC 3 cut(s) 317, 543, 671
BbvI GCAGC 3 cut(s) 110, 113, 385
BccI CCATC 1 cut(s) 760
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 424
BclI TGATCA 1 cut(s) 1111
BcnI CCSGG 1 cut(s) 1229
BfaI CTAG 1 cut(s) 1246
BfuAI ACCTGC 1 cut(s) 534
BisI GCNGC 7 cut(s) 99, 102, 249, 399, 681, 821, 1238
BlsI GCNGC 7 cut(s) 100, 103, 250, 400, 682, 822, 1239
Bme1390I CCNGG 2 cut(s) 424, 1229
Bme18I GGWCC 1 cut(s) 888
BmgT120I GGNCC 2 cut(s) 318, 888
BmrFI CCNGG 2 cut(s) 424, 1229
BmrI ACTGGG 1 cut(s) 1008
BmsI GCATC 3 cut(s) 630, 678, 1224
BmuI ACTGGG 1 cut(s) 1008
BpiI GAAGAC 3 cut(s) 317, 543, 671
BpuEI CTTGAG 1 cut(s) 782
BpuMI CCSGG 1 cut(s) 1229
BsaJI CCNNGG 4 cut(s) 9, 249, 423, 609
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 794, 824
Bsc4I CCNNNNNNNGG 5 cut(s) 423, 471, 568, 885, 1213
Bse1I ACTGG 2 cut(s) 1003, 1148
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 4 cut(s) 9, 249, 423, 609
BseGI GGATG 1 cut(s) 700
BseLI CCNNNNNNNGG 5 cut(s) 423, 471, 568, 885, 1213
BseMII CTCAG 3 cut(s) 282, 830, 1044
BseNI ACTGG 2 cut(s) 1003, 1148
BseRI GAGGAG 7 cut(s) 135, 138, 141, 269, 272, 383, 815
BseXI GCAGC 3 cut(s) 110, 113, 385
BsgI GTGCAG 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 2 cut(s) 248, 320
BsiSI CCGG 2 cut(s) 162, 1228
BslI CCNNNNNNNGG 5 cut(s) 423, 471, 568, 885, 1213
BsnI GGCC 2 cut(s) 248, 320
Bsp143I GATC 2 cut(s) 906, 1111
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 6 cut(s) 249, 251, 401, 584, 680, 820
BspANI GGCC 2 cut(s) 248, 320
BspCNI CTCAG 3 cut(s) 283, 831, 1045
BspFNI CGCG 1 cut(s) 251
BspMI ACCTGC 1 cut(s) 534
BspPI GGATC 1 cut(s) 914
BsrI ACTGG 2 cut(s) 1003, 1148
BssECI CCNNGG 4 cut(s) 9, 249, 423, 609
BssMI GATC 2 cut(s) 906, 1111
BssT1I CCWWGG 2 cut(s) 9, 609
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 2 cut(s) 542, 670
BstC8I GCNNGC 4 cut(s) 96, 246, 396, 1165
BstDEI CTNAG 3 cut(s) 291, 839, 1053
BstDSI CCRYGG 2 cut(s) 9, 249
BstF5I GGATG 1 cut(s) 700
BstFNI CGCG 1 cut(s) 251
BstHHI GCGC 1 cut(s) 608
BstKTI GATC 2 cut(s) 909, 1114
BstMBI GATC 2 cut(s) 906, 1111
BstMWI GCNNNNNNNGC 2 cut(s) 91, 1099
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 3 cut(s) 244, 436, 1167
BstSCI CCNGG 2 cut(s) 422, 1227
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 3 cut(s) 110, 113, 385
BstV2I GAAGAC 3 cut(s) 317, 543, 671
BstX2I RGATCY 1 cut(s) 906
BstYI RGATCY 1 cut(s) 906
BsuRI GGCC 2 cut(s) 248, 320
BtgI CCRYGG 2 cut(s) 9, 249
BtsCI GGATG 1 cut(s) 700
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 148
BveI ACCTGC 1 cut(s) 534
Cac8I GCNNGC 4 cut(s) 96, 246, 396, 1165
CaiI CAGNNNCTG 1 cut(s) 998
CfoI GCGC 1 cut(s) 608
Cfr13I GGNCC 2 cut(s) 318, 888
Cfr42I CCGCGG 1 cut(s) 252
CseI GACGC 2 cut(s) 1169, 1229
CviAII CATG 4 cut(s) 10, 241, 433, 1164
DdeI CTNAG 3 cut(s) 291, 839, 1053
DpnI GATC 2 cut(s) 908, 1113
DpnII GATC 2 cut(s) 906, 1111
EaeI YGGCCR 1 cut(s) 246
Eco130I CCWWGG 2 cut(s) 9, 609
Eco47I GGWCC 1 cut(s) 888
Eco57I CTGAAG 2 cut(s) 480, 1088
EcoRII CCWGG 1 cut(s) 422
EcoT14I CCWWGG 2 cut(s) 9, 609
ErhI CCWWGG 2 cut(s) 9, 609
FaeI CATG 4 cut(s) 13, 244, 436, 1167
FatI CATG 4 cut(s) 9, 240, 432, 1163
FauNDI CATATG 1 cut(s) 884
FbaI TGATCA 1 cut(s) 1111
Fnu4HI GCNGC 7 cut(s) 99, 102, 249, 399, 681, 821, 1238
FokI GGATG 1 cut(s) 707
Fsp4HI GCNGC 7 cut(s) 99, 102, 249, 399, 681, 821, 1238
FspBI CTAG 1 cut(s) 1246
GlaI GCGC 1 cut(s) 607
GluI GCNGC 7 cut(s) 99, 102, 249, 399, 681, 821, 1238
HaeIII GGCC 2 cut(s) 248, 320
HapII CCGG 2 cut(s) 162, 1228
HgaI GACGC 2 cut(s) 1169, 1229
HhaI GCGC 1 cut(s) 608
Hin1II CATG 4 cut(s) 13, 244, 436, 1167
Hin6I GCGC 1 cut(s) 606
HinP1I GCGC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 7 cut(s) 80, 269, 373, 404, 494, 1055, 1144
HpaII CCGG 2 cut(s) 162, 1228
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 7 cut(s) 286, 442, 701, 796, 892, 994, 1054
Hpy188III TCNNGA 2 cut(s) 626, 650
Hpy8I GTNNAC 1 cut(s) 625
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 5 cut(s) 203, 501, 509, 997, 1204
HpyCH4III ACNGT 2 cut(s) 542, 670
HpyCH4V TGCA 4 cut(s) 141, 436, 933, 1237
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 1099
HpyF3I CTNAG 3 cut(s) 291, 839, 1053
Hsp92II CATG 4 cut(s) 13, 244, 436, 1167
HspAI GCGC 1 cut(s) 606
Ksp22I TGATCA 1 cut(s) 1111
KspI CCGCGG 1 cut(s) 252
Kzo9I GATC 2 cut(s) 906, 1111
LmnI GCTCC 1 cut(s) 787
Lsp1109I GCAGC 3 cut(s) 110, 113, 385
LweI GCATC 3 cut(s) 630, 678, 1224
MaeI CTAG 1 cut(s) 1246
MaeIII GTNAC 2 cut(s) 551, 1241
MalI GATC 2 cut(s) 908, 1113
MboI GATC 2 cut(s) 906, 1111
MboII GAAGA 8 cut(s) 187, 190, 322, 524, 548, 676, 714, 1184
MflI RGATCY 1 cut(s) 906
MlyI GAGTC 1 cut(s) 1064
MmeI TCCRAC 1 cut(s) 432
MseI TTAA 4 cut(s) 39, 590, 734, 1185
MslI CAYNNNNRTG 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 2 cut(s) 162, 1228
MspR9I CCNGG 2 cut(s) 424, 1229
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 91, 1099
NciI CCSGG 1 cut(s) 1229
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 884
NdeII GATC 2 cut(s) 906, 1111
NlaIII CATG 4 cut(s) 13, 244, 436, 1167
NmuCI GTSAC 1 cut(s) 1241
NspI RCATGY 3 cut(s) 244, 436, 1167
PaeI GCATGC 1 cut(s) 1167
PfeI GAWTC 6 cut(s) 80, 269, 373, 404, 494, 1144
PflMI CCANNNNNTGG 1 cut(s) 885
PfoI TCCNGGA 1 cut(s) 1227
PkrI GCNGC 7 cut(s) 100, 103, 250, 400, 682, 822, 1239
PleI GAGTC 1 cut(s) 1063
PpsI GAGTC 1 cut(s) 1063
PsiI TTATAA 2 cut(s) 972, 1124
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspPI GGNCC 2 cut(s) 318, 888
PstNI CAGNNNCTG 1 cut(s) 998
PsuI RGATCY 1 cut(s) 906
RseI CAYNNNNRTG 1 cut(s) 630
SacII CCGCGG 1 cut(s) 252
SaqAI TTAA 4 cut(s) 39, 590, 734, 1185
SatI GCNGC 7 cut(s) 99, 102, 249, 399, 681, 821, 1238
Sau3AI GATC 2 cut(s) 906, 1111
Sau96I GGNCC 2 cut(s) 318, 888
SchI GAGTC 1 cut(s) 1064
ScrFI CCNGG 2 cut(s) 424, 1229
SfaNI GCATC 3 cut(s) 630, 678, 1224
Sfr303I CCGCGG 1 cut(s) 252
SgrBI CCGCGG 1 cut(s) 252
SinI GGWCC 1 cut(s) 888
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
SphI GCATGC 1 cut(s) 1167
SsiI CCGC 6 cut(s) 249, 251, 401, 584, 680, 820
SspI AATATT 1 cut(s) 988
SspMI CTAG 1 cut(s) 1246
StyD4I CCNGG 2 cut(s) 422, 1227
StyI CCWWGG 2 cut(s) 9, 609
TaaI ACNGT 2 cut(s) 542, 670
TaqI TCGA 4 cut(s) 89, 118, 201, 1089
TauI GCSGC 3 cut(s) 251, 683, 823
TfiI GAWTC 6 cut(s) 80, 269, 373, 404, 494, 1144
Tru1I TTAA 4 cut(s) 39, 590, 734, 1185
Tru9I TTAA 4 cut(s) 39, 590, 734, 1185
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 1241
TseI GCWGC 4 cut(s) 98, 101, 398, 1237
Tsp45I GTSAC 1 cut(s) 1241
TspDTI ATGAA 4 cut(s) 19, 744, 861, 907
TspGWI ACGGA 3 cut(s) 148, 190, 539
TspRI CASTG 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 885
VpaK11BI GGWCC 1 cut(s) 888
XapI RAATTY 4 cut(s) 296, 575, 959, 1080
XceI RCATGY 3 cut(s) 244, 436, 1167
XspI CTAG 1 cut(s) 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.