Rw0G021960

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01020
OG0004997
Physical Location & Seq
Reverse (-)
5513 .. 10561
5049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G021960.1

Sequence Viewer

Length: 1263 bp
ATGTTGTCCATGGTCTTATGCGAGAACTTCATCTTTGTTAACAACCACAACAAGCAGACTTTGTTTTCGCACAAAATTGATTCGCAGTCGAGACTCGCAGCAGCAGCAGTTGAGGTGGGTCGAGGAGGAGGAGGAGGACGGAGGTGCAGTGGGTTCGTTGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCGGAGGAGGAGGAGGAGGAGAACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGGGCCGAAGCAATCGTGGATTTGAGGGAAGCACAGCAGGATATGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCGAACTCGTCCTCGTCCTGGGAATACTTGCAGTCTGATATTCCTACCGAAACCGAAGCCGAAAAGGGGGTGCTTTACTCTCTCACCGATTCCCTTCTTGGAAGGGAAGATGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCCCTCAATTCGGCTAAATTTTTGGCGGTTTTAATAATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCAGCAGAGATACTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATGGAGAGAGCCAGATTTCAGCTTGAGATGGAAATTGGTAAAGCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTCATTTGGGATCTCATTGTTCATTCTTTTATACTGCAACCAGAGTAAAGTAGCTTTGCTGAAATTTACAGGTTATAAGATAATAAATAATATTTCAGATACTGGGAAGGCATTTCTAATTATACTCATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAGACTTTGATAGAAATTTTTGTCGAGCATTATGGGCTTCAGGTTGATCAAGCAGTTATAACCATTTTTATCTGCTTGATTCCAGTTATTATTGACGCATGCGTGAAACTTTGGCTGTTTAAGTTTCTTCCGAGATTATCCCCGAAGGTGGCAAACATATTCCGGGAGATGCAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

420

Amino Acids

48.73

Weight (kDa)

4.91

Isoelectric Point (pI)

53.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 189 - 418 4.7e-76 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 987, 1139
Acc36I ACCTGC 1 cut(s) 549
AccB7I CCANNNNNTGG 1 cut(s) 900
AccII CGCG 1 cut(s) 257
AciI CCGC 5 cut(s) 255, 257, 416, 599, 835
AclWI GGATC 1 cut(s) 929
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 4 cut(s) 311, 590, 974, 1095
AcuI CTGAAG 1 cut(s) 1103
AfiI CCNNNNNNNGG 5 cut(s) 438, 486, 583, 900, 1228
AgsI TTSAA 3 cut(s) 235, 734, 875
AjnI CCWGG 1 cut(s) 437
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 7 cut(s) 198, 305, 730, 775, 797, 853, 965
AluI AGCT 7 cut(s) 198, 305, 730, 775, 797, 853, 965
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 1 cut(s) 929
AlwNI CAGNNNCTG 1 cut(s) 1013
AoxI GGCC 2 cut(s) 252, 333
ApeKI GCWGC 6 cut(s) 98, 101, 104, 413, 695, 1252
ApoI RAATTY 4 cut(s) 311, 590, 974, 1095
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
AspLEI GCGC 1 cut(s) 623
AspS9I GGNCC 2 cut(s) 333, 903
AsuC2I CCSGG 1 cut(s) 1244
AsuHPI GGTGA 1 cut(s) 496
AsuII TTCGAA 1 cut(s) 422
AvaII GGWCC 1 cut(s) 903
BbsI GAAGAC 3 cut(s) 332, 558, 686
BbvI GCAGC 5 cut(s) 110, 113, 116, 400, 707
BccI CCATC 1 cut(s) 775
BcgI CGANNNNNNTGC 2 cut(s) 136, 170
BciT130I CCWGG 1 cut(s) 439
BclI TGATCA 1 cut(s) 1126
BcnI CCSGG 1 cut(s) 1244
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 1 cut(s) 1261
BfuAI ACCTGC 1 cut(s) 549
BisI GCNGC 8 cut(s) 99, 102, 105, 255, 414, 696, 836, 1253
BlsI GCNGC 8 cut(s) 100, 103, 106, 256, 415, 697, 837, 1254
Bme1390I CCNGG 2 cut(s) 439, 1244
Bme18I GGWCC 1 cut(s) 903
BmgT120I GGNCC 2 cut(s) 333, 903
BmrFI CCNGG 2 cut(s) 439, 1244
BmrI ACTGGG 1 cut(s) 1023
BmsI GCATC 2 cut(s) 645, 1239
BmuI ACTGGG 1 cut(s) 1023
BpiI GAAGAC 3 cut(s) 332, 558, 686
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 797
BpuMI CCSGG 1 cut(s) 1244
BsaJI CCNNGG 4 cut(s) 9, 255, 438, 624
BsaXI ACNNNNNCTCC 4 cut(s) 133, 163, 809, 839
Bsc4I CCNNNNNNNGG 5 cut(s) 438, 486, 583, 900, 1228
Bse1I ACTGG 3 cut(s) 711, 1018, 1163
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 4 cut(s) 9, 255, 438, 624
BseGI GGATG 1 cut(s) 715
BseLI CCNNNNNNNGG 5 cut(s) 438, 486, 583, 900, 1228
BseMII CTCAG 3 cut(s) 297, 845, 1059
BseNI ACTGG 3 cut(s) 711, 1018, 1163
BseXI GCAGC 5 cut(s) 110, 113, 116, 400, 707
BsgI GTGCAG 1 cut(s) 166
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 2 cut(s) 254, 335
BsiSI CCGG 2 cut(s) 168, 1243
BslI CCNNNNNNNGG 5 cut(s) 438, 486, 583, 900, 1228
BsmAI GTCTC 1 cut(s) 85
BsnI GGCC 2 cut(s) 254, 335
Bsp119I TTCGAA 1 cut(s) 422
Bsp143I GATC 2 cut(s) 921, 1126
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 5 cut(s) 255, 257, 416, 599, 835
BspANI GGCC 2 cut(s) 254, 335
BspCNI CTCAG 3 cut(s) 298, 846, 1060
BspFNI CGCG 1 cut(s) 257
BspMI ACCTGC 1 cut(s) 549
BspPI GGATC 1 cut(s) 929
BspT104I TTCGAA 1 cut(s) 422
BsrI ACTGG 3 cut(s) 711, 1018, 1163
BssECI CCNNGG 4 cut(s) 9, 255, 438, 624
BssMI GATC 2 cut(s) 921, 1126
BssT1I CCWWGG 2 cut(s) 9, 624
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 2 cut(s) 557, 685
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 3 cut(s) 252, 411, 1180
BstDEI CTNAG 3 cut(s) 306, 854, 1068
BstDSI CCRYGG 2 cut(s) 9, 255
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 1 cut(s) 257
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 2 cut(s) 924, 1129
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 2 cut(s) 921, 1126
BstMWI GCNNNNNNNGC 2 cut(s) 104, 1114
BstNI CCWGG 1 cut(s) 439
BstNSI RCATGY 2 cut(s) 250, 1182
BstSCI CCNGG 2 cut(s) 437, 1242
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 5 cut(s) 110, 113, 116, 400, 707
BstV2I GAAGAC 3 cut(s) 332, 558, 686
BstX2I RGATCY 1 cut(s) 921
BstYI RGATCY 1 cut(s) 921
BsuRI GGCC 2 cut(s) 254, 335
BtgI CCRYGG 2 cut(s) 9, 255
BtsCI GGATG 1 cut(s) 715
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 154
BveI ACCTGC 1 cut(s) 549
Cac8I GCNNGC 3 cut(s) 252, 411, 1180
CaiI CAGNNNCTG 1 cut(s) 1013
CfoI GCGC 1 cut(s) 623
Cfr13I GGNCC 2 cut(s) 333, 903
Cfr42I CCGCGG 1 cut(s) 258
CseI GACGC 2 cut(s) 1184, 1244
CviAII CATG 3 cut(s) 10, 247, 1179
DdeI CTNAG 3 cut(s) 306, 854, 1068
DpnI GATC 2 cut(s) 923, 1128
DpnII GATC 2 cut(s) 921, 1126
EaeI YGGCCR 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 9, 624
Eco47I GGWCC 1 cut(s) 903
Eco57I CTGAAG 1 cut(s) 1103
EcoRII CCWGG 1 cut(s) 437
EcoT14I CCWWGG 2 cut(s) 9, 624
ErhI CCWWGG 2 cut(s) 9, 624
FaeI CATG 3 cut(s) 13, 250, 1182
FatI CATG 3 cut(s) 9, 246, 1178
FauNDI CATATG 1 cut(s) 899
FbaI TGATCA 1 cut(s) 1126
Fnu4HI GCNGC 8 cut(s) 99, 102, 105, 255, 414, 696, 836, 1253
FokI GGATG 1 cut(s) 722
Fsp4HI GCNGC 8 cut(s) 99, 102, 105, 255, 414, 696, 836, 1253
FspBI CTAG 1 cut(s) 1261
GlaI GCGC 1 cut(s) 622
GluI GCNGC 8 cut(s) 99, 102, 105, 255, 414, 696, 836, 1253
HaeIII GGCC 2 cut(s) 254, 335
HapII CCGG 2 cut(s) 168, 1243
HgaI GACGC 2 cut(s) 1184, 1244
HhaI GCGC 1 cut(s) 623
Hin1II CATG 3 cut(s) 13, 250, 1182
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 8 cut(s) 80, 93, 284, 388, 419, 509, 1070, 1159
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 2 cut(s) 168, 1243
HphI GGTGA 1 cut(s) 496
Hpy166II GTNNAC 2 cut(s) 40, 640
Hpy188I TCNGA 8 cut(s) 301, 457, 716, 811, 907, 1009, 1069, 1212
Hpy188III TCNNGA 3 cut(s) 90, 641, 665
Hpy8I GTNNAC 2 cut(s) 40, 640
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 5 cut(s) 209, 516, 524, 1012, 1219
HpyCH4III ACNGT 2 cut(s) 557, 685
HpyCH4V TGCA 5 cut(s) 147, 451, 695, 948, 1252
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 1114
HpyF3I CTNAG 3 cut(s) 306, 854, 1068
Hsp92II CATG 3 cut(s) 13, 250, 1182
HspAI GCGC 1 cut(s) 621
Ksp22I TGATCA 1 cut(s) 1126
KspAI GTTAAC 1 cut(s) 40
KspI CCGCGG 1 cut(s) 258
Kzo9I GATC 2 cut(s) 921, 1126
LmnI GCTCC 1 cut(s) 802
Lsp1109I GCAGC 5 cut(s) 110, 113, 116, 400, 707
LweI GCATC 2 cut(s) 645, 1239
MaeI CTAG 1 cut(s) 1261
MaeIII GTNAC 2 cut(s) 566, 1256
MalI GATC 2 cut(s) 923, 1128
MboI GATC 2 cut(s) 921, 1126
MboII GAAGA 8 cut(s) 193, 196, 337, 539, 563, 691, 729, 1199
MflI RGATCY 1 cut(s) 921
MlyI GAGTC 2 cut(s) 87, 1079
MseI TTAA 4 cut(s) 39, 605, 749, 1200
MslI CAYNNNNRTG 1 cut(s) 645
MspA1I CMGCKG 1 cut(s) 257
MspI CCGG 2 cut(s) 168, 1243
MspR9I CCNGG 2 cut(s) 439, 1244
MvaI CCWGG 1 cut(s) 439
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 2 cut(s) 104, 1114
NciI CCSGG 1 cut(s) 1244
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 899
NdeII GATC 2 cut(s) 921, 1126
NlaIII CATG 3 cut(s) 13, 250, 1182
NmuCI GTSAC 1 cut(s) 1256
NspI RCATGY 2 cut(s) 250, 1182
NspV TTCGAA 1 cut(s) 422
PaeI GCATGC 1 cut(s) 1182
PfeI GAWTC 6 cut(s) 80, 284, 388, 419, 509, 1159
PflMI CCANNNNNTGG 1 cut(s) 900
PfoI TCCNGGA 1 cut(s) 1242
PkrI GCNGC 8 cut(s) 100, 103, 106, 256, 415, 697, 837, 1254
PleI GAGTC 2 cut(s) 87, 1078
PpsI GAGTC 2 cut(s) 87, 1078
PsiI TTATAA 2 cut(s) 987, 1139
Psp6I CCWGG 1 cut(s) 437
PspGI CCWGG 1 cut(s) 437
PspPI GGNCC 2 cut(s) 333, 903
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 1 cut(s) 921
RseI CAYNNNNRTG 1 cut(s) 645
SacII CCGCGG 1 cut(s) 258
SaqAI TTAA 4 cut(s) 39, 605, 749, 1200
SatI GCNGC 8 cut(s) 99, 102, 105, 255, 414, 696, 836, 1253
Sau3AI GATC 2 cut(s) 921, 1126
Sau96I GGNCC 2 cut(s) 333, 903
SchI GAGTC 2 cut(s) 87, 1079
ScrFI CCNGG 2 cut(s) 439, 1244
SfaNI GCATC 2 cut(s) 645, 1239
Sfr303I CCGCGG 1 cut(s) 258
SfuI TTCGAA 1 cut(s) 422
SgrBI CCGCGG 1 cut(s) 258
SinI GGWCC 1 cut(s) 903
SmiMI CAYNNNNRTG 1 cut(s) 645
SmlI CTYRAG 1 cut(s) 776
SmoI CTYRAG 1 cut(s) 776
SphI GCATGC 1 cut(s) 1182
SsiI CCGC 5 cut(s) 255, 257, 416, 599, 835
SspI AATATT 1 cut(s) 1003
SspMI CTAG 1 cut(s) 1261
StyD4I CCNGG 2 cut(s) 437, 1242
StyI CCWWGG 2 cut(s) 9, 624
TaaI ACNGT 2 cut(s) 557, 685
TaqI TCGA 5 cut(s) 89, 121, 207, 422, 1104
TauI GCSGC 2 cut(s) 257, 838
TfiI GAWTC 6 cut(s) 80, 284, 388, 419, 509, 1159
Tru1I TTAA 4 cut(s) 39, 605, 749, 1200
Tru9I TTAA 4 cut(s) 39, 605, 749, 1200
TscAI CASTG 1 cut(s) 154
TseFI GTSAC 1 cut(s) 1256
TseI GCWGC 6 cut(s) 98, 101, 104, 413, 695, 1252
Tsp45I GTSAC 1 cut(s) 1256
TspDTI ATGAA 4 cut(s) 19, 759, 876, 922
TspGWI ACGGA 3 cut(s) 154, 196, 554
TspRI CASTG 1 cut(s) 154
Van91I CCANNNNNTGG 1 cut(s) 900
VpaK11BI GGWCC 1 cut(s) 903
XapI RAATTY 4 cut(s) 311, 590, 974, 1095
XceI RCATGY 2 cut(s) 250, 1182
XspI CTAG 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.