Rh4AG001400

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004997
Physical Location & Seq
Forward (+)
250046 .. 255823
5778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG001400.1

Sequence Viewer

Length: 978 bp
ATGTTGTCCATGGTCTTATGCGAAAACTTCATCTTTGTTAACAACCACAACAAGCAGACTTTGTTTTCGCACAAAATTGATTCGCAGTCGAGACTCGCAGCAGCAGTTGAGGTGGGTCGAGGAGGAGGACGGAGGTGCAGTGGGTTCGTTGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCTGAGGAGGAAGAGGAGGAGAACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGGGCCGAAGCAATCGTGGATTTGAGGGAAGCACAGCAGGATATGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCGAACTCGTCTCCGTCGTCCTCGTCCTGGTCCTGGGAATACATGCAGTCTGATATTCCTACCGAAACTGAAGCCGAAAAGGGGTTGCTTTACTCTCTCACCGATTCCCTTCTTGGAAGGGAAGATGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCCCTCAATTCGGCTAAATTTTTGGCGGTTTTAATAATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCAGCAGAGATGCTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTAATGAATTAAAAATTGAGAGAGCAAGATTTCAGCTTGAGATGGAAATTGGTAAAGCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTTATTTGGGATCTCATTATTCATTCTTTTATACTGCAACCAGAGTAAAGTGAGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

325

Amino Acids

37.99

Weight (kDa)

4.73

Isoelectric Point (pI)

55.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 191 - 323 5.3e-27 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 555
AccB7I CCANNNNNTGG 1 cut(s) 906
AciI CCGC 4 cut(s) 246, 407, 605, 841
AclWI GGATC 1 cut(s) 935
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 2 cut(s) 302, 596
AcuI CTGAAG 1 cut(s) 501
AfiI CCNNNNNNNGG 5 cut(s) 438, 444, 492, 589, 906
AgsI TTSAA 3 cut(s) 226, 740, 881
AjnI CCWGG 2 cut(s) 437, 443
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 6 cut(s) 189, 296, 736, 781, 803, 859
AluI AGCT 6 cut(s) 189, 296, 736, 781, 803, 859
Alw26I GTCTC 2 cut(s) 85, 426
AlwI GGATC 1 cut(s) 935
AoxI GGCC 2 cut(s) 243, 324
ApeKI GCWGC 4 cut(s) 98, 101, 404, 701
ApoI RAATTY 2 cut(s) 302, 596
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
AspLEI GCGC 1 cut(s) 629
AspS9I GGNCC 3 cut(s) 324, 441, 909
AsuHPI GGTGA 1 cut(s) 502
AsuII TTCGAA 1 cut(s) 413
AvaII GGWCC 2 cut(s) 441, 909
BbsI GAAGAC 3 cut(s) 323, 564, 692
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 4 cut(s) 110, 113, 391, 713
BccI CCATC 1 cut(s) 781
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 2 cut(s) 439, 445
BcoDI GTCTC 2 cut(s) 85, 426
BfuAI ACCTGC 1 cut(s) 555
BisI GCNGC 6 cut(s) 99, 102, 246, 405, 702, 842
BlsI GCNGC 6 cut(s) 100, 103, 247, 406, 703, 843
Bme1390I CCNGG 2 cut(s) 439, 445
Bme18I GGWCC 2 cut(s) 441, 909
BmgT120I GGNCC 3 cut(s) 324, 441, 909
BmrFI CCNGG 2 cut(s) 439, 445
BmsI GCATC 2 cut(s) 651, 699
BpiI GAAGAC 3 cut(s) 323, 564, 692
Bpu10I CCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 803
BsaJI CCNNGG 3 cut(s) 9, 444, 630
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 815, 845
Bsc4I CCNNNNNNNGG 5 cut(s) 438, 444, 492, 589, 906
BseBI CCWGG 2 cut(s) 439, 445
BseDI CCNNGG 3 cut(s) 9, 444, 630
BseGI GGATG 1 cut(s) 721
BseLI CCNNNNNNNGG 5 cut(s) 438, 444, 492, 589, 906
BseMII CTCAG 3 cut(s) 240, 288, 851
BseRI GAGGAG 7 cut(s) 135, 138, 266, 275, 278, 389, 836
BseXI GCAGC 4 cut(s) 110, 113, 391, 713
BsgI GTGCAG 1 cut(s) 157
BshFI GGCC 2 cut(s) 245, 326
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 5 cut(s) 438, 444, 492, 589, 906
BsmAI GTCTC 2 cut(s) 85, 426
BsmBI CGTCTC 1 cut(s) 426
BsnI GGCC 2 cut(s) 245, 326
Bsp119I TTCGAA 1 cut(s) 413
Bsp143I GATC 1 cut(s) 927
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 4 cut(s) 246, 407, 605, 841
BspANI GGCC 2 cut(s) 245, 326
BspCNI CTCAG 3 cut(s) 241, 289, 852
BspMI ACCTGC 1 cut(s) 555
BspPI GGATC 1 cut(s) 935
BspT104I TTCGAA 1 cut(s) 413
BssECI CCNNGG 3 cut(s) 9, 444, 630
BssMI GATC 1 cut(s) 927
BssT1I CCWWGG 2 cut(s) 9, 630
Bst2UI CCWGG 2 cut(s) 439, 445
Bst4CI ACNGT 2 cut(s) 563, 691
Bst6I CTCTTC 1 cut(s) 252
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 243, 402
BstDEI CTNAG 3 cut(s) 249, 297, 860
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 721
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 1 cut(s) 930
BstMAI GTCTC 2 cut(s) 85, 426
BstMBI GATC 1 cut(s) 927
BstNI CCWGG 2 cut(s) 439, 445
BstNSI RCATGY 2 cut(s) 241, 457
BstSCI CCNGG 2 cut(s) 437, 443
BstV1I GCAGC 4 cut(s) 110, 113, 391, 713
BstV2I GAAGAC 3 cut(s) 323, 564, 692
BstX2I RGATCY 1 cut(s) 927
BstYI RGATCY 1 cut(s) 927
BsuRI GGCC 2 cut(s) 245, 326
BtgI CCRYGG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 721
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
BveI ACCTGC 1 cut(s) 555
Cac8I GCNNGC 2 cut(s) 243, 402
CfoI GCGC 1 cut(s) 629
Cfr13I GGNCC 3 cut(s) 324, 441, 909
CviAII CATG 3 cut(s) 10, 238, 454
DdeI CTNAG 3 cut(s) 249, 297, 860
DpnI GATC 1 cut(s) 929
DpnII GATC 1 cut(s) 927
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 9, 630
Eco47I GGWCC 2 cut(s) 441, 909
Eco57I CTGAAG 1 cut(s) 501
EcoRII CCWGG 2 cut(s) 437, 443
EcoT14I CCWWGG 2 cut(s) 9, 630
ErhI CCWWGG 2 cut(s) 9, 630
Esp3I CGTCTC 1 cut(s) 426
FaeI CATG 3 cut(s) 13, 241, 457
FatI CATG 3 cut(s) 9, 237, 453
FauNDI CATATG 1 cut(s) 905
Fnu4HI GCNGC 6 cut(s) 99, 102, 246, 405, 702, 842
FokI GGATG 1 cut(s) 728
Fsp4HI GCNGC 6 cut(s) 99, 102, 246, 405, 702, 842
GlaI GCGC 1 cut(s) 628
GluI GCNGC 6 cut(s) 99, 102, 246, 405, 702, 842
HaeIII GGCC 2 cut(s) 245, 326
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 629
Hin1II CATG 3 cut(s) 13, 241, 457
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 6 cut(s) 80, 93, 275, 379, 410, 515
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 2 cut(s) 40, 646
Hpy188I TCNGA 5 cut(s) 292, 463, 722, 817, 913
Hpy188III TCNNGA 3 cut(s) 90, 647, 671
Hpy8I GTNNAC 2 cut(s) 40, 646
Hpy99I CGWCG 2 cut(s) 203, 430
HpyAV CCTTC 3 cut(s) 200, 522, 530
HpyCH4III ACNGT 2 cut(s) 563, 691
HpyCH4V TGCA 4 cut(s) 138, 457, 701, 954
HpyF3I CTNAG 3 cut(s) 249, 297, 860
Hsp92II CATG 3 cut(s) 13, 241, 457
HspAI GCGC 1 cut(s) 627
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 1 cut(s) 927
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 4 cut(s) 110, 113, 391, 713
LweI GCATC 2 cut(s) 651, 699
MaeIII GTNAC 1 cut(s) 572
MalI GATC 1 cut(s) 929
MboI GATC 1 cut(s) 927
MboII GAAGA 8 cut(s) 184, 187, 269, 328, 545, 569, 697, 735
MflI RGATCY 1 cut(s) 927
MlyI GAGTC 1 cut(s) 87
MseI TTAA 4 cut(s) 39, 611, 747, 755
MslI CAYNNNNRTG 1 cut(s) 651
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 2 cut(s) 439, 445
MvaI CCWGG 2 cut(s) 439, 445
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 905
NdeII GATC 1 cut(s) 927
NlaIII CATG 3 cut(s) 13, 241, 457
NspI RCATGY 2 cut(s) 241, 457
NspV TTCGAA 1 cut(s) 413
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PfeI GAWTC 5 cut(s) 80, 275, 379, 410, 515
PflMI CCANNNNNTGG 1 cut(s) 906
PkrI GCNGC 6 cut(s) 100, 103, 247, 406, 703, 843
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 437, 443
PspGI CCWGG 2 cut(s) 437, 443
PspPI GGNCC 3 cut(s) 324, 441, 909
PsuI RGATCY 1 cut(s) 927
RseI CAYNNNNRTG 1 cut(s) 651
SaqAI TTAA 4 cut(s) 39, 611, 747, 755
SatI GCNGC 6 cut(s) 99, 102, 246, 405, 702, 842
Sau3AI GATC 1 cut(s) 927
Sau96I GGNCC 3 cut(s) 324, 441, 909
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 2 cut(s) 439, 445
SfaNI GCATC 2 cut(s) 651, 699
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 2 cut(s) 441, 909
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
SsiI CCGC 4 cut(s) 246, 407, 605, 841
StyD4I CCNGG 2 cut(s) 437, 443
StyI CCWWGG 2 cut(s) 9, 630
TaaI ACNGT 2 cut(s) 563, 691
TaqI TCGA 4 cut(s) 89, 118, 198, 413
TauI GCSGC 2 cut(s) 248, 844
TfiI GAWTC 5 cut(s) 80, 275, 379, 410, 515
Tru1I TTAA 4 cut(s) 39, 611, 747, 755
Tru9I TTAA 4 cut(s) 39, 611, 747, 755
TscAI CASTG 1 cut(s) 145
TseI GCWGC 4 cut(s) 98, 101, 404, 701
TspDTI ATGAA 4 cut(s) 19, 765, 882, 928
TspGWI ACGGA 4 cut(s) 145, 187, 414, 560
TspRI CASTG 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 906
VpaK11BI GGWCC 2 cut(s) 441, 909
XapI RAATTY 2 cut(s) 302, 596
XceI RCATGY 2 cut(s) 241, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.