FvH4_4g00180

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004997
Physical Location & Seq
Forward (+)
174885 .. 179130
4246 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00180.t7

Sequence Viewer

Length: 1251 bp
ATGTTGTCCATGGTCTTGTGCGAGAACTTGATTTTTGTTAACACCAACCGCAAGAACGCCTTTTTCTCCCACAAAATTGATTCGCACTCGAGACTAGCAGCAGCTCAAGTTGAAGCTCAAGCTCAAGTTCAAGTTCAAGGTAGGAGGTGCAGTGGGCTCGTTGCCAATGCCGGAAAAAGAAACCGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGATGGCAACTGGCTTGGTTTGAAGGACGATGACATGCTGGCGGCGGAGGAGGAGGAAGAGAGCTTGGATGGCTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGAGCGGAGGCAATCGTGGATTTGAGGGAAGCACAGCAAGATATAAGCAACGAGGAGAATCGCAAGTGGGAGGACTGGCTCGCCGTGGATTCATCCTCCTCGTGGGAATTCGTGCAGTCTGATGATACTCCTCCTACCCAAACTGAAGCCGAAAAAGGAATGCTTTACTCTCTCACCGATTCCCTTCTCGGAAGGGAAGACGATGACCTGCTCTATGAAGACCGTGTCTTCCGTTACGCCTCCCTCAATTCGGCTAAATTTTTGGCGCTGTTAATCATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCTTTTCTCGACAGATATGTGAAGACCGTGCCACTTGCAGCAGAGATGCTGGACGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATTGAGAGAGCAAGATTTCAGCTTGAGATGGAAATCGGTAAATCTCCTCCCCTCTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATAGAAGAGCATTTGCCAACATATGGTCAGATATGTTATTTGGGATCTCATTGTTCATTCTTTTATACTGCAACCAGAGTAAAGTAGCTTTGCTGAAATTTACAGGCTATAAGATAATAAATAATATTTCAGATACTGGGAAGGCATTTCTAATTATACTCATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAGACTTTGTTAGAAATTTTTGTTGAGCATTATGGGCTTCAGATTGATCAATCAGCCATAACCATTTTTATCTGCTTGATTCCAGTTATCATTGACGCATGCGTGAAACTTTGGCTGTTTAAGTTCCTTCCAAGATTATCCCCAAAGGTGGCAAACATATTCCGCGAGATGCAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

417

Amino Acids

48.41

Weight (kDa)

4.93

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 185 - 395 3.1e-68 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 702
Acc36I ACCTGC 1 cut(s) 537
AccB7I CCANNNNNTGG 1 cut(s) 888
AccBSI CCGCTC 1 cut(s) 326
AccII CGCG 1 cut(s) 1233
AciI CCGC 6 cut(s) 49, 257, 260, 326, 823, 1231
AclWI GGATC 1 cut(s) 917
AcsI RAATTY 5 cut(s) 302, 428, 578, 962, 1083
AcuI CTGAAG 2 cut(s) 486, 1091
AcyI GRCGYC 1 cut(s) 699
AfiI CCNNNNNNNGG 4 cut(s) 423, 571, 888, 1216
AgsI TTSAA 6 cut(s) 113, 131, 137, 238, 722, 863
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 1 cut(s) 917
AlwNI CAGNNNCTG 1 cut(s) 1001
Ama87I CYCGRG 1 cut(s) 88
ApeKI GCWGC 4 cut(s) 98, 101, 683, 1240
ApoI RAATTY 5 cut(s) 302, 428, 578, 962, 1083
AspLEI GCGC 2 cut(s) 589, 611
AsuHPI GGTGA 1 cut(s) 487
AvaI CYCGRG 1 cut(s) 88
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 1 cut(s) 421
BbsI GAAGAC 5 cut(s) 323, 525, 541, 546, 674
BbvI GCAGC 3 cut(s) 110, 113, 695
BccI CCATC 3 cut(s) 212, 278, 763
BceAI ACGGC 1 cut(s) 389
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BclI TGATCA 1 cut(s) 1114
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 2 cut(s) 95, 1249
BfoI RGCGCY 1 cut(s) 590
BfuAI ACCTGC 1 cut(s) 537
BisI GCNGC 6 cut(s) 99, 102, 258, 684, 824, 1241
BlsI GCNGC 6 cut(s) 100, 103, 259, 685, 825, 1242
BmeT110I CYCGRG 1 cut(s) 88
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 3 cut(s) 633, 681, 1227
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 5 cut(s) 323, 525, 541, 546, 674
BpuEI CTTGAG 4 cut(s) 90, 102, 108, 785
BsaBI GATNNNNATC 1 cut(s) 773
BsaHI GRCGYC 1 cut(s) 699
BsaJI CCNNGG 3 cut(s) 9, 405, 612
BsaWI WCCGGW 1 cut(s) 183
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 797, 827
Bsc4I CCNNNNNNNGG 4 cut(s) 423, 571, 888, 1216
Bse1I ACTGG 4 cut(s) 230, 401, 1006, 1151
Bse8I GATNNNNATC 1 cut(s) 773
BseDI CCNNGG 3 cut(s) 9, 405, 612
BseGI GGATG 3 cut(s) 223, 289, 413
BseJI GATNNNNATC 1 cut(s) 773
BseLI CCNNNNNNNGG 4 cut(s) 423, 571, 888, 1216
BseMII CTCAG 3 cut(s) 288, 833, 1047
BseNI ACTGG 4 cut(s) 230, 401, 1006, 1151
BseRI GAGGAG 7 cut(s) 278, 281, 389, 409, 441, 777, 818
BseXI GCAGC 3 cut(s) 110, 113, 695
BsgI GTGCAG 2 cut(s) 169, 455
Bsh1236I CGCG 1 cut(s) 1233
BsiHKCI CYCGRG 1 cut(s) 88
BsiSI CCGG 2 cut(s) 171, 184
BslI CCNNNNNNNGG 4 cut(s) 423, 571, 888, 1216
BsmAI GTCTC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 486
BsoBI CYCGRG 1 cut(s) 88
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 2 cut(s) 909, 1114
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 6 cut(s) 49, 257, 260, 326, 823, 1231
BspCNI CTCAG 3 cut(s) 289, 834, 1048
BspFNI CGCG 1 cut(s) 1233
BspMI ACCTGC 1 cut(s) 537
BspPI GGATC 1 cut(s) 917
BspQI GCTCTTC 1 cut(s) 865
BsrBI CCGCTC 1 cut(s) 326
BsrI ACTGG 4 cut(s) 230, 401, 1006, 1151
BssECI CCNNGG 3 cut(s) 9, 405, 612
BssMI GATC 2 cut(s) 909, 1114
BssNI GRCGYC 1 cut(s) 699
BssSI CACGAG 1 cut(s) 421
BssT1I CCWWGG 2 cut(s) 9, 612
Bst2BI CACGAG 1 cut(s) 421
Bst4CI ACNGT 2 cut(s) 545, 673
Bst6I CTCTTC 2 cut(s) 267, 865
BstACI GRCGYC 1 cut(s) 699
BstC8I GCNNGC 3 cut(s) 255, 402, 1168
BstDEI CTNAG 3 cut(s) 297, 842, 1056
BstDSI CCRYGG 2 cut(s) 9, 405
BstF5I GGATG 3 cut(s) 223, 289, 413
BstFNI CGCG 1 cut(s) 1233
BstH2I RGCGCY 1 cut(s) 590
BstHHI GCGC 2 cut(s) 589, 611
BstKTI GATC 2 cut(s) 912, 1117
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 2 cut(s) 909, 1114
BstMWI GCNNNNNNNGC 2 cut(s) 285, 1102
BstNSI RCATGY 2 cut(s) 253, 1170
BstUI CGCG 1 cut(s) 1233
BstV1I GCAGC 3 cut(s) 110, 113, 695
BstV2I GAAGAC 5 cut(s) 323, 525, 541, 546, 674
BstX2I RGATCY 1 cut(s) 909
BstYI RGATCY 1 cut(s) 909
BtgI CCRYGG 2 cut(s) 9, 405
BtsCI GGATG 3 cut(s) 223, 289, 413
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 157
BveI ACCTGC 1 cut(s) 537
Cac8I GCNNGC 3 cut(s) 255, 402, 1168
CaiI CAGNNNCTG 1 cut(s) 1001
CfoI GCGC 2 cut(s) 589, 611
CseI GACGC 2 cut(s) 1172, 1232
CviAII CATG 3 cut(s) 10, 250, 1167
DdeI CTNAG 3 cut(s) 297, 842, 1056
DpnI GATC 2 cut(s) 911, 1116
DpnII GATC 2 cut(s) 909, 1114
Eam1104I CTCTTC 2 cut(s) 267, 865
EarI CTCTTC 2 cut(s) 267, 865
EciI GGCGGA 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 9, 612
Eco24I GRGCYC 1 cut(s) 159
Eco57I CTGAAG 2 cut(s) 486, 1091
Eco88I CYCGRG 1 cut(s) 88
EcoRI GAATTC 1 cut(s) 428
EcoT14I CCWWGG 2 cut(s) 9, 612
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 2 cut(s) 9, 612
FaeI CATG 3 cut(s) 13, 253, 1170
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 468, 500
FatI CATG 3 cut(s) 9, 249, 1166
FauNDI CATATG 1 cut(s) 887
FbaI TGATCA 1 cut(s) 1114
Fnu4HI GCNGC 6 cut(s) 99, 102, 258, 684, 824, 1241
FokI GGATG 3 cut(s) 230, 296, 400
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 6 cut(s) 99, 102, 258, 684, 824, 1241
FspBI CTAG 2 cut(s) 95, 1249
GlaI GCGC 2 cut(s) 588, 610
GluI GCNGC 6 cut(s) 99, 102, 258, 684, 824, 1241
HaeII RGCGCY 1 cut(s) 590
HapII CCGG 2 cut(s) 171, 184
HgaI GACGC 2 cut(s) 1172, 1232
HhaI GCGC 2 cut(s) 589, 611
Hin1I GRCGYC 1 cut(s) 699
Hin1II CATG 3 cut(s) 13, 253, 1170
Hin6I GCGC 2 cut(s) 587, 609
HinP1I GCGC 2 cut(s) 587, 609
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 6 cut(s) 80, 379, 410, 500, 1058, 1147
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 2 cut(s) 171, 184
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 2 cut(s) 40, 628
Hpy188I TCNGA 9 cut(s) 292, 442, 512, 704, 799, 895, 997, 1057, 1110
Hpy188III TCNNGA 3 cut(s) 90, 629, 653
Hpy8I GTNNAC 2 cut(s) 40, 628
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 5 cut(s) 232, 507, 515, 1000, 1205
HpyCH4III ACNGT 2 cut(s) 545, 673
HpyCH4IV ACGT 1 cut(s) 699
HpyCH4V TGCA 5 cut(s) 150, 436, 683, 936, 1240
HpyF10VI GCNNNNNNNGC 2 cut(s) 285, 1102
HpyF3I CTNAG 3 cut(s) 297, 842, 1056
HpySE526I ACGT 1 cut(s) 699
Hsp92I GRCGYC 1 cut(s) 699
Hsp92II CATG 3 cut(s) 13, 253, 1170
HspAI GCGC 2 cut(s) 587, 609
Ksp22I TGATCA 1 cut(s) 1114
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 2 cut(s) 909, 1114
LguI GCTCTTC 1 cut(s) 865
Lsp1109I GCAGC 3 cut(s) 110, 113, 695
LweI GCATC 3 cut(s) 633, 681, 1227
MaeI CTAG 2 cut(s) 95, 1249
MaeII ACGT 1 cut(s) 699
MaeIII GTNAC 2 cut(s) 554, 1244
MalI GATC 2 cut(s) 911, 1116
MbiI CCGCTC 1 cut(s) 326
MboI GATC 2 cut(s) 909, 1114
MflI RGATCY 1 cut(s) 909
MhlI GDGCHC 1 cut(s) 159
MlyI GAGTC 1 cut(s) 1067
MseI TTAA 4 cut(s) 39, 593, 737, 1188
MslI CAYNNNNRTG 1 cut(s) 633
MspI CCGG 2 cut(s) 171, 184
Mva1269I GAATGC 1 cut(s) 486
MvnI CGCG 1 cut(s) 1233
MwoI GCNNNNNNNGC 2 cut(s) 285, 1102
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 887
NdeII GATC 2 cut(s) 909, 1114
NlaIII CATG 3 cut(s) 13, 253, 1170
NmuCI GTSAC 1 cut(s) 1244
NspI RCATGY 2 cut(s) 253, 1170
PaeI GCATGC 1 cut(s) 1170
PaeR7I CTCGAG 1 cut(s) 88
PciSI GCTCTTC 1 cut(s) 865
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 5 cut(s) 80, 379, 410, 500, 1147
PflFI GACNNNGTC 1 cut(s) 545
PflMI CCANNNNNTGG 1 cut(s) 888
PkrI GCNGC 6 cut(s) 100, 103, 259, 685, 825, 1242
PleI GAGTC 1 cut(s) 1066
PpsI GAGTC 1 cut(s) 1066
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 1 cut(s) 909
PsyI GACNNNGTC 1 cut(s) 545
RseI CAYNNNNRTG 1 cut(s) 633
SapI GCTCTTC 1 cut(s) 865
SaqAI TTAA 4 cut(s) 39, 593, 737, 1188
SatI GCNGC 6 cut(s) 99, 102, 258, 684, 824, 1241
Sau3AI GATC 2 cut(s) 909, 1114
SchI GAGTC 1 cut(s) 1067
SduI GDGCHC 1 cut(s) 159
SfaNI GCATC 3 cut(s) 633, 681, 1227
Sfr274I CTCGAG 1 cut(s) 88
SlaI CTCGAG 1 cut(s) 88
SmiMI CAYNNNNRTG 1 cut(s) 633
SmlI CTYRAG 5 cut(s) 88, 105, 117, 123, 764
SmoI CTYRAG 5 cut(s) 88, 105, 117, 123, 764
SphI GCATGC 1 cut(s) 1170
SsiI CCGC 6 cut(s) 49, 257, 260, 326, 823, 1231
SspI AATATT 1 cut(s) 991
SspMI CTAG 2 cut(s) 95, 1249
StyI CCWWGG 2 cut(s) 9, 612
TaaI ACNGT 2 cut(s) 545, 673
TaiI ACGT 1 cut(s) 702
TaqI TCGA 3 cut(s) 89, 210, 654
TauI GCSGC 2 cut(s) 260, 826
TfiI GAWTC 5 cut(s) 80, 379, 410, 500, 1147
Tru1I TTAA 4 cut(s) 39, 593, 737, 1188
Tru9I TTAA 4 cut(s) 39, 593, 737, 1188
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 1244
TseI GCWGC 4 cut(s) 98, 101, 683, 1240
Tsp45I GTSAC 1 cut(s) 1244
TspDTI ATGAA 5 cut(s) 402, 552, 747, 864, 910
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 1 cut(s) 157
Tth111I GACNNNGTC 1 cut(s) 545
Van91I CCANNNNNTGG 1 cut(s) 888
XapI RAATTY 5 cut(s) 302, 428, 578, 962, 1083
XceI RCATGY 2 cut(s) 253, 1170
XhoI CTCGAG 1 cut(s) 88
XspI CTAG 2 cut(s) 95, 1249
ZraI GACGTC 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.