MD04G1001800.v1.1

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0004997
Physical Location & Seq
Forward (+)
224525 .. 230742
6218 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1001800.v1.1.491

Sequence Viewer

Length: 1287 bp
ATGTTAATCATGTCCTCCCCCGCCGTCTTCTGCAAAACCTTTATCTTTGTTAACCCCAAGCTCAGGCTGCTCAGTCGCAACACCTTCATTCTGCACAGAAATTGGTCGACTGCACTGGTGGTTGATACAAGGCGGTGGAGGTCTGCCGCTGCGGGGCACATTCCAAATGCTGGTGGCAAAAGGAAAAGGAAAAGGACTTGGTGGCAAACATTCTTTTTCGACGACGACGGGAATTGGCTTGGTTTGAAGGAGGACGACATGCTGGCGACGGAGGAGGAGGACTTGGATGGATCCTCCGCAGCTGCCTCCACGGAAAAAGGTGAAGAAGCTGAGAAATTTGAGGCGTGGAAGACGAGGGCAGAAGCAATCGTGGAGCTGAGGGAAGCGCAGCAGGATTTGACCAACGAGGAGAACCGCAAGTGGGAGGATTGGTTTGTGGATTCTAATTCTAAATCTACTTCCTTCTGGGAAGCATCTGTAGAGTCGGATCCTGCTCAAGAAAGGGGATTGCTTTACTCTCTCAGCAATTTCGTCCTCGGAAGGGAAGAAGATGATGATGAGGACATGCTCTATGAAGACCGTGTTTTTCGCTATGCCTCACTCAATTCGGCTAAATTTTTAGCAGTATTAATAATCATACCCTGGGCCTTGGATTTTGTGGTTCACGACTATGTTCTGATGCCCTTTTTAGACAGATATGTGAAGACCGTACCACTTGCAGCACAGTTGCTTGATGTCAGAAGAAATCAAAAGCTCCAAATGATTGATGAATTAAAAATGGAGAGAGCAAGATTTCATCTTGAGATGGAGATTGGTAAATCTCCACCGCTTTCTGATGACGAGGTTTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTAGAGAATCGTAAAGCATTTGCCAACATATGGTCAGATATGATATTCGGGATCTCATTATTCATTCTTTTATACAGCAATCAGAATAAAGTAGCATTGGTGAAGTTTACTGGTTATAAGATCATAAATAATATTTCAGATACCGGGAAGGCATTTCTAATTATACTTATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAAACATTGTTAGAAATTTTTGTTGAGCATTATGGGCTTGAGGTTGATCAGTCAGCCGTAACCTTTTTCATTTGCCTGATTCCAGTCATCATGGATGCATGTGTCAAACTATGGCTGTTTAAATTCCTTCCAAGATTATCCCCAAAGGTGACAAACATATTCCGGGAAATGAAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

429

Amino Acids

50.08

Weight (kDa)

5.35

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 197 - 426 2e-76 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1011
AccB7I CCANNNNNTGG 2 cut(s) 170, 924
AccI GTMKAC 1 cut(s) 107
AciI CCGC 8 cut(s) 21, 133, 147, 152, 297, 415, 827, 859
AclWI GGATC 5 cut(s) 285, 298, 482, 495, 953
AcsI RAATTY 4 cut(s) 335, 614, 1119, 1226
AfaI GTAC 1 cut(s) 711
AfiI CCNNNNNNNGG 6 cut(s) 63, 153, 170, 421, 541, 924
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 1 cut(s) 641
AluBI AGCT 6 cut(s) 61, 302, 329, 376, 754, 877
AluI AGCT 6 cut(s) 61, 302, 329, 376, 754, 877
AlwI GGATC 5 cut(s) 285, 298, 482, 495, 953
AoxI GGCC 1 cut(s) 645
ApeKI GCWGC 6 cut(s) 67, 149, 299, 302, 388, 719
ApoI RAATTY 4 cut(s) 335, 614, 1119, 1226
AseI ATTAAT 1 cut(s) 629
AspLEI GCGC 1 cut(s) 388
AspS9I GGNCC 1 cut(s) 645
AsuC2I CCSGG 2 cut(s) 1039, 1268
AsuHPI GGTGA 3 cut(s) 332, 1006, 1264
BaeGI GKGCMC 1 cut(s) 159
BamHI GGATCC 2 cut(s) 290, 487
BbsI GAAGAC 4 cut(s) 19, 356, 582, 710
BbvCI CCTCAGC 1 cut(s) 377
BbvI GCAGC 6 cut(s) 54, 136, 289, 311, 400, 731
BccI CCATC 2 cut(s) 281, 799
BceAI ACGGC 2 cut(s) 8, 1145
BciT130I CCWGG 1 cut(s) 643
BclI TGATCA 1 cut(s) 1150
BcnI CCSGG 2 cut(s) 1039, 1268
BfaI CTAG 1 cut(s) 1285
BfmI CTRYAG 1 cut(s) 477
BisI GCNGC 8 cut(s) 68, 147, 150, 300, 303, 389, 720, 860
BlsI GCNGC 8 cut(s) 69, 148, 151, 301, 304, 390, 721, 861
Bme1390I CCNGG 3 cut(s) 643, 1039, 1268
BmgT120I GGNCC 1 cut(s) 645
BmiI GGNNCC 2 cut(s) 292, 489
BmrFI CCNGG 3 cut(s) 643, 1039, 1268
BmsI GCATC 3 cut(s) 482, 669, 1189
BpiI GAAGAC 4 cut(s) 19, 356, 582, 710
Bpu10I CCTNAGC 2 cut(s) 62, 377
BpuEI CTTGAG 3 cut(s) 480, 821, 1163
BpuMI CCSGG 2 cut(s) 1039, 1268
BsaJI CCNNGG 5 cut(s) 309, 535, 641, 642, 648
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 6 cut(s) 63, 153, 170, 421, 541, 924
Bse1I ACTGG 3 cut(s) 120, 1009, 1187
BseBI CCWGG 1 cut(s) 643
BseDI CCNNGG 5 cut(s) 309, 535, 641, 642, 648
BseGI GGATG 2 cut(s) 292, 1204
BseLI CCNNNNNNNGG 6 cut(s) 63, 153, 170, 421, 541, 924
BseMII CTCAG 7 cut(s) 76, 85, 321, 368, 535, 869, 1083
BseNI ACTGG 3 cut(s) 120, 1009, 1187
BseRI GAGGAG 3 cut(s) 287, 290, 422
BseSI GKGCMC 1 cut(s) 159
BseXI GCAGC 6 cut(s) 54, 136, 289, 311, 400, 731
BsgI GTGCAG 2 cut(s) 77, 96
BshFI GGCC 1 cut(s) 647
BsiSI CCGG 2 cut(s) 1038, 1267
BslI CCNNNNNNNGG 6 cut(s) 63, 153, 170, 421, 541, 924
BsnI GGCC 1 cut(s) 647
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 5 cut(s) 290, 487, 945, 1014, 1150
BspACI CCGC 8 cut(s) 21, 133, 147, 152, 297, 415, 827, 859
BspANI GGCC 1 cut(s) 647
BspCNI CTCAG 7 cut(s) 75, 84, 322, 369, 534, 870, 1084
BspLI GGNNCC 2 cut(s) 292, 489
BspPI GGATC 5 cut(s) 285, 298, 482, 495, 953
BsrI ACTGG 3 cut(s) 120, 1009, 1187
BssECI CCNNGG 5 cut(s) 309, 535, 641, 642, 648
BssMI GATC 5 cut(s) 290, 487, 945, 1014, 1150
BssT1I CCWWGG 1 cut(s) 648
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 3 cut(s) 581, 709, 726
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 7 cut(s) 62, 71, 330, 377, 521, 878, 1092
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 2 cut(s) 292, 1204
BstHHI GCGC 1 cut(s) 388
BstKTI GATC 5 cut(s) 293, 490, 948, 1017, 1153
BstMBI GATC 5 cut(s) 290, 487, 945, 1014, 1150
BstMWI GCNNNNNNNGC 2 cut(s) 67, 1138
BstNI CCWGG 1 cut(s) 643
BstNSI RCATGY 3 cut(s) 262, 568, 1206
BstSCI CCNGG 3 cut(s) 641, 1037, 1266
BstSFI CTRYAG 1 cut(s) 477
BstSLI GKGCMC 1 cut(s) 159
BstV1I GCAGC 6 cut(s) 54, 136, 289, 311, 400, 731
BstV2I GAAGAC 4 cut(s) 19, 356, 582, 710
BstX2I RGATCY 3 cut(s) 290, 487, 945
BstYI RGATCY 3 cut(s) 290, 487, 945
BsuRI GGCC 1 cut(s) 647
BtgI CCRYGG 1 cut(s) 309
BtsCI GGATG 2 cut(s) 292, 1204
BtsIMutI CAGTG 1 cut(s) 113
Cac8I GCNNGC 1 cut(s) 264
CfoI GCGC 1 cut(s) 388
Cfr13I GGNCC 1 cut(s) 645
CseI GACGC 1 cut(s) 1268
Csp6I GTAC 1 cut(s) 710
CviAII CATG 5 cut(s) 10, 259, 565, 1195, 1203
CviQI GTAC 1 cut(s) 710
DdeI CTNAG 7 cut(s) 62, 71, 330, 377, 521, 878, 1092
DpnI GATC 5 cut(s) 292, 489, 947, 1016, 1152
DpnII GATC 5 cut(s) 290, 487, 945, 1014, 1150
DraI TTTAAA 1 cut(s) 1225
Eco130I CCWWGG 1 cut(s) 648
EcoRII CCWGG 1 cut(s) 641
EcoT14I CCWWGG 1 cut(s) 648
EcoT22I ATGCAT 1 cut(s) 1204
ErhI CCWWGG 1 cut(s) 648
FaeI CATG 5 cut(s) 13, 262, 568, 1198, 1206
FatI CATG 5 cut(s) 9, 258, 564, 1194, 1202
FauI CCCGC 2 cut(s) 28, 145
FauNDI CATATG 1 cut(s) 923
FbaI TGATCA 1 cut(s) 1150
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 8 cut(s) 68, 147, 150, 300, 303, 389, 720, 860
FokI GGATG 2 cut(s) 299, 1211
Fsp4HI GCNGC 8 cut(s) 68, 147, 150, 300, 303, 389, 720, 860
FspBI CTAG 1 cut(s) 1285
GlaI GCGC 1 cut(s) 387
GluI GCNGC 8 cut(s) 68, 147, 150, 300, 303, 389, 720, 860
HaeIII GGCC 1 cut(s) 647
HapII CCGG 2 cut(s) 1038, 1267
HgaI GACGC 1 cut(s) 1268
HhaI GCGC 1 cut(s) 388
Hin1II CATG 5 cut(s) 13, 262, 568, 1198, 1206
Hin6I GCGC 1 cut(s) 386
HinP1I GCGC 1 cut(s) 386
HincII GTYRAC 2 cut(s) 52, 108
HindII GTYRAC 2 cut(s) 52, 108
HinfI GANTC 5 cut(s) 440, 482, 901, 1094, 1183
HpaI GTTAAC 1 cut(s) 52
HpaII CCGG 2 cut(s) 1038, 1267
HphI GGTGA 3 cut(s) 332, 1006, 1264
Hpy166II GTNNAC 4 cut(s) 52, 108, 664, 1002
Hpy188I TCNGA 9 cut(s) 487, 539, 678, 740, 835, 931, 978, 1033, 1093
Hpy188III TCNNGA 4 cut(s) 497, 665, 800, 943
Hpy8I GTNNAC 4 cut(s) 52, 108, 664, 1002
Hpy99I CGWCG 4 cut(s) 224, 227, 230, 271
HpyAV CCTTC 6 cut(s) 94, 241, 472, 534, 1036, 1241
HpyCH4III ACNGT 3 cut(s) 581, 709, 726
HpyCH4V TGCA 5 cut(s) 33, 94, 113, 719, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 1138
HpyF3I CTNAG 7 cut(s) 62, 71, 330, 377, 521, 878, 1092
Hsp92II CATG 5 cut(s) 13, 262, 568, 1198, 1206
HspAI GCGC 1 cut(s) 386
Ksp22I TGATCA 1 cut(s) 1150
KspAI GTTAAC 1 cut(s) 52
Kzo9I GATC 5 cut(s) 290, 487, 945, 1014, 1150
LmnI GCTCC 2 cut(s) 373, 759
Lsp1109I GCAGC 6 cut(s) 54, 136, 289, 311, 400, 731
LweI GCATC 3 cut(s) 482, 669, 1189
MaeI CTAG 1 cut(s) 1285
MaeIII GTNAC 3 cut(s) 1162, 1252, 1280
MalI GATC 5 cut(s) 292, 489, 947, 1016, 1152
MboI GATC 5 cut(s) 290, 487, 945, 1014, 1150
MboII GAAGA 8 cut(s) 19, 335, 361, 557, 560, 587, 715, 753
MflI RGATCY 3 cut(s) 290, 487, 945
MhlI GDGCHC 1 cut(s) 159
MlyI GAGTC 2 cut(s) 491, 1103
MmeI TCCRAC 1 cut(s) 465
Mph1103I ATGCAT 1 cut(s) 1204
MseI TTAA 5 cut(s) 5, 51, 629, 773, 1224
MslI CAYNNNNRTG 1 cut(s) 669
MspA1I CMGCKG 2 cut(s) 149, 302
MspI CCGG 2 cut(s) 1038, 1267
MspR9I CCNGG 3 cut(s) 643, 1039, 1268
MvaI CCWGG 1 cut(s) 643
MwoI GCNNNNNNNGC 2 cut(s) 67, 1138
NciI CCSGG 2 cut(s) 1039, 1268
NdeI CATATG 1 cut(s) 923
NdeII GATC 5 cut(s) 290, 487, 945, 1014, 1150
NlaIII CATG 5 cut(s) 13, 262, 568, 1198, 1206
NlaIV GGNNCC 2 cut(s) 292, 489
NmuCI GTSAC 2 cut(s) 1252, 1280
NsiI ATGCAT 1 cut(s) 1204
NspI RCATGY 3 cut(s) 262, 568, 1206
PasI CCCWGGG 1 cut(s) 642
PfeI GAWTC 3 cut(s) 440, 901, 1183
PflMI CCANNNNNTGG 2 cut(s) 170, 924
PfoI TCCNGGA 1 cut(s) 1266
PkrI GCNGC 8 cut(s) 69, 148, 151, 301, 304, 390, 721, 861
PleI GAGTC 2 cut(s) 490, 1102
PpsI GAGTC 2 cut(s) 490, 1102
PshBI ATTAAT 1 cut(s) 629
PsiI TTATAA 1 cut(s) 1011
Psp6I CCWGG 1 cut(s) 641
PspGI CCWGG 1 cut(s) 641
PspN4I GGNNCC 2 cut(s) 292, 489
PspPI GGNCC 1 cut(s) 645
PsuI RGATCY 3 cut(s) 290, 487, 945
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 711
RsaNI GTAC 1 cut(s) 710
RseI CAYNNNNRTG 1 cut(s) 669
SalI GTCGAC 1 cut(s) 106
SaqAI TTAA 5 cut(s) 5, 51, 629, 773, 1224
SatI GCNGC 8 cut(s) 68, 147, 150, 300, 303, 389, 720, 860
Sau3AI GATC 5 cut(s) 290, 487, 945, 1014, 1150
Sau96I GGNCC 1 cut(s) 645
SchI GAGTC 2 cut(s) 491, 1103
ScrFI CCNGG 3 cut(s) 643, 1039, 1268
SduI GDGCHC 1 cut(s) 159
SfaNI GCATC 3 cut(s) 482, 669, 1189
SfcI CTRYAG 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 669
SmlI CTYRAG 3 cut(s) 495, 800, 1142
SmoI CTYRAG 3 cut(s) 495, 800, 1142
SsiI CCGC 8 cut(s) 21, 133, 147, 152, 297, 415, 827, 859
SspI AATATT 1 cut(s) 1027
SspMI CTAG 1 cut(s) 1285
StyD4I CCNGG 3 cut(s) 641, 1037, 1266
StyI CCWWGG 1 cut(s) 648
TaaI ACNGT 3 cut(s) 581, 709, 726
TaqI TCGA 2 cut(s) 107, 219
TauI GCSGC 2 cut(s) 149, 862
TfiI GAWTC 3 cut(s) 440, 901, 1183
Tru1I TTAA 5 cut(s) 5, 51, 629, 773, 1224
Tru9I TTAA 5 cut(s) 5, 51, 629, 773, 1224
TscAI CASTG 1 cut(s) 120
TseFI GTSAC 2 cut(s) 1252, 1280
TseI GCWGC 6 cut(s) 67, 149, 299, 302, 388, 719
Tsp45I GTSAC 2 cut(s) 1252, 1280
TspDTI ATGAA 7 cut(s) 76, 588, 783, 785, 900, 946, 1162
TspGWI ACGGA 2 cut(s) 284, 326
TspRI CASTG 1 cut(s) 120
Van91I CCANNNNNTGG 2 cut(s) 170, 924
VspI ATTAAT 1 cut(s) 629
XapI RAATTY 4 cut(s) 335, 614, 1119, 1226
XceI RCATGY 3 cut(s) 262, 568, 1206
XmiI GTMKAC 1 cut(s) 107
XspI CTAG 1 cut(s) 1285
Zsp2I ATGCAT 1 cut(s) 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.