RLG00000010270

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0004997
Physical Location & Seq
Reverse (-)
64987036 .. 64992179
5144 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010270

Sequence Viewer

Length: 1263 bp
ATGTTGTCCATGGTCTTATGCGAGAACTTAATCTTTGTTAACAACCACAACAAGCAGACTTTGTTTTCGCACAGAATTGATTCGCAGTCGAGACTCGCAGCAGCAGTTGAGGTGGGTCGAGGAGGAGGACGGAGGTGCAGTGGGTTCGTCGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCGGAGGAGGAGGACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCATGGAAGACAAGGGCCGAAGCAATAGTGGATTTGAGGGAAGCACAGCAGGATATGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCGAACTCGGCTCCGTCGTCCTCGTCCTCGTCCTGGGAATACATGCCGTCTGATATTCCTACCGAAACTGAAGCCGAAAAGGGGGTGCTTTACTCTCTCACCGATTCCCTTCTTGGAAGGGACCGGGAAGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCACTCAATTCGGCTAAATTTTTGGCGGTTTTAATTATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCAGCAGAGATGCTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATTGAGAGAGCAAGATTTCAGCTTGAGATGGAAATTGGTAAATCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTTATTTGGGATCTCATTATTCATTCTTTTATACTGCAACCAGAGTAAAGTAGCTTTGCTGAAATTTACAGGTTATAAGATAATAAATAATATTTCAGATACTGGGAAGGCATTCTTAATTATACTCATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAGACTTTGATAGAAATTTTTGTTGAGCATTATGGGCTTCAGGTTGATCAATCAGTTATAACCATTTTTATCTGCTTGATTCCGGTTATCATTGACGCATGCGTGAAACTTTGGCTGTTTAAGTTCCTTCCAAGATTATCCCCGAAGGTGGCAAACATATTCCGGGAGATGCAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

421

Amino Acids

48.78

Weight (kDa)

5.01

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 189 - 418 1.1e-76 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 987, 1139
Acc36I ACCTGC 1 cut(s) 549
AccB7I CCANNNNNTGG 1 cut(s) 900
AccII CGCG 1 cut(s) 248
AciI CCGC 5 cut(s) 246, 248, 398, 599, 835
AclWI GGATC 1 cut(s) 929
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 4 cut(s) 293, 590, 974, 1095
AcuI CTGAAG 2 cut(s) 492, 1103
AfiI CCNNNNNNNGG 4 cut(s) 435, 483, 900, 1228
AgsI TTSAA 3 cut(s) 226, 734, 875
AjnI CCWGG 1 cut(s) 434
AluBI AGCT 6 cut(s) 189, 287, 730, 775, 853, 965
AluI AGCT 6 cut(s) 189, 287, 730, 775, 853, 965
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 1 cut(s) 929
AlwNI CAGNNNCTG 1 cut(s) 1013
AoxI GGCC 2 cut(s) 243, 315
ApeKI GCWGC 5 cut(s) 98, 101, 395, 695, 1252
ApoI RAATTY 4 cut(s) 293, 590, 974, 1095
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
Asp700I GAANNNNTTC 2 cut(s) 79, 1022
AspLEI GCGC 1 cut(s) 623
AspS9I GGNCC 3 cut(s) 315, 523, 903
AsuC2I CCSGG 2 cut(s) 527, 1244
AsuHPI GGTGA 1 cut(s) 493
AsuII TTCGAA 1 cut(s) 404
AvaII GGWCC 2 cut(s) 523, 903
BbsI GAAGAC 3 cut(s) 314, 558, 686
BbvI GCAGC 4 cut(s) 110, 113, 382, 707
BccI CCATC 1 cut(s) 775
BceAI ACGGC 1 cut(s) 433
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 436
BclI TGATCA 1 cut(s) 1126
BcnI CCSGG 2 cut(s) 527, 1244
BcoDI GTCTC 1 cut(s) 85
BfaI CTAG 1 cut(s) 1261
BfuAI ACCTGC 1 cut(s) 549
BisI GCNGC 7 cut(s) 99, 102, 246, 396, 696, 836, 1253
BlsI GCNGC 7 cut(s) 100, 103, 247, 397, 697, 837, 1254
Bme1390I CCNGG 3 cut(s) 436, 527, 1244
Bme18I GGWCC 2 cut(s) 523, 903
BmgT120I GGNCC 3 cut(s) 315, 523, 903
BmiI GGNNCC 2 cut(s) 414, 524
BmrFI CCNGG 3 cut(s) 436, 527, 1244
BmrI ACTGGG 1 cut(s) 1023
BmsI GCATC 3 cut(s) 645, 693, 1239
BmuI ACTGGG 1 cut(s) 1023
BpiI GAAGAC 3 cut(s) 314, 558, 686
Bpu14I TTCGAA 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 797
BpuMI CCSGG 2 cut(s) 527, 1244
BsaJI CCNNGG 4 cut(s) 9, 246, 435, 624
BsaWI WCCGGW 1 cut(s) 1162
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 809, 839
Bsc4I CCNNNNNNNGG 4 cut(s) 435, 483, 900, 1228
Bse1I ACTGG 1 cut(s) 1018
BseBI CCWGG 1 cut(s) 436
BseDI CCNNGG 4 cut(s) 9, 246, 435, 624
BseGI GGATG 1 cut(s) 715
BseLI CCNNNNNNNGG 4 cut(s) 435, 483, 900, 1228
BseMII CTCAG 3 cut(s) 279, 845, 1059
BseNI ACTGG 1 cut(s) 1018
BseRI GAGGAG 6 cut(s) 135, 138, 266, 269, 380, 830
BseXI GCAGC 4 cut(s) 110, 113, 382, 707
BsgI GTGCAG 1 cut(s) 157
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 2 cut(s) 245, 317
BsiSI CCGG 4 cut(s) 159, 526, 1163, 1243
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 4 cut(s) 435, 483, 900, 1228
BsmAI GTCTC 1 cut(s) 85
BsmFI GGGAC 1 cut(s) 536
BsmI GAATGC 1 cut(s) 1022
BsnI GGCC 2 cut(s) 245, 317
Bsp119I TTCGAA 1 cut(s) 404
Bsp143I GATC 2 cut(s) 921, 1126
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 5 cut(s) 246, 248, 398, 599, 835
BspANI GGCC 2 cut(s) 245, 317
BspCNI CTCAG 3 cut(s) 280, 846, 1060
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 2 cut(s) 414, 524
BspMI ACCTGC 1 cut(s) 549
BspPI GGATC 1 cut(s) 929
BspT104I TTCGAA 1 cut(s) 404
BsrI ACTGG 1 cut(s) 1018
BssECI CCNNGG 4 cut(s) 9, 246, 435, 624
BssMI GATC 2 cut(s) 921, 1126
BssT1I CCWWGG 2 cut(s) 9, 624
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 557, 685
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 243, 393, 1180
BstDEI CTNAG 3 cut(s) 288, 854, 1068
BstDSI CCRYGG 2 cut(s) 9, 246
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 2 cut(s) 924, 1129
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 2 cut(s) 921, 1126
BstMWI GCNNNNNNNGC 1 cut(s) 1114
BstNI CCWGG 1 cut(s) 436
BstNSI RCATGY 3 cut(s) 241, 448, 1182
BstSCI CCNGG 3 cut(s) 434, 525, 1242
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 4 cut(s) 110, 113, 382, 707
BstV2I GAAGAC 3 cut(s) 314, 558, 686
BstX2I RGATCY 1 cut(s) 921
BstYI RGATCY 1 cut(s) 921
BsuRI GGCC 2 cut(s) 245, 317
BtgI CCRYGG 2 cut(s) 9, 246
BtsCI GGATG 1 cut(s) 715
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
BveI ACCTGC 1 cut(s) 549
Cac8I GCNNGC 3 cut(s) 243, 393, 1180
CaiI CAGNNNCTG 1 cut(s) 1013
CfoI GCGC 1 cut(s) 623
Cfr13I GGNCC 3 cut(s) 315, 523, 903
Cfr42I CCGCGG 1 cut(s) 249
CseI GACGC 2 cut(s) 1184, 1244
CviAII CATG 5 cut(s) 10, 238, 303, 445, 1179
DdeI CTNAG 3 cut(s) 288, 854, 1068
DpnI GATC 2 cut(s) 923, 1128
DpnII GATC 2 cut(s) 921, 1126
EaeI YGGCCR 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 9, 624
Eco47I GGWCC 2 cut(s) 523, 903
Eco57I CTGAAG 2 cut(s) 492, 1103
EcoRII CCWGG 1 cut(s) 434
EcoT14I CCWWGG 2 cut(s) 9, 624
ErhI CCWWGG 2 cut(s) 9, 624
FaeI CATG 5 cut(s) 13, 241, 306, 448, 1182
FalI AAGNNNNNCTT 2 cut(s) 1010, 1042
FaqI GGGAC 1 cut(s) 536
FatI CATG 5 cut(s) 9, 237, 302, 444, 1178
FauNDI CATATG 1 cut(s) 899
FbaI TGATCA 1 cut(s) 1126
Fnu4HI GCNGC 7 cut(s) 99, 102, 246, 396, 696, 836, 1253
FokI GGATG 1 cut(s) 722
Fsp4HI GCNGC 7 cut(s) 99, 102, 246, 396, 696, 836, 1253
FspBI CTAG 1 cut(s) 1261
GlaI GCGC 1 cut(s) 622
GluI GCNGC 7 cut(s) 99, 102, 246, 396, 696, 836, 1253
HaeIII GGCC 2 cut(s) 245, 317
HapII CCGG 4 cut(s) 159, 526, 1163, 1243
HgaI GACGC 2 cut(s) 1184, 1244
HhaI GCGC 1 cut(s) 623
Hin1II CATG 5 cut(s) 13, 241, 306, 448, 1182
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 728
HinfI GANTC 8 cut(s) 80, 93, 266, 370, 401, 506, 1070, 1159
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 4 cut(s) 159, 526, 1163, 1243
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 2 cut(s) 40, 640
Hpy188I TCNGA 7 cut(s) 283, 454, 716, 811, 907, 1009, 1069
Hpy188III TCNNGA 3 cut(s) 90, 641, 665
Hpy8I GTNNAC 2 cut(s) 40, 640
Hpy99I CGWCG 3 cut(s) 152, 203, 421
HpyAV CCTTC 6 cut(s) 200, 513, 521, 1012, 1217, 1219
HpyCH4III ACNGT 2 cut(s) 557, 685
HpyCH4V TGCA 4 cut(s) 138, 695, 948, 1252
HpyF10VI GCNNNNNNNGC 1 cut(s) 1114
HpyF3I CTNAG 3 cut(s) 288, 854, 1068
Hsp92II CATG 5 cut(s) 13, 241, 306, 448, 1182
HspAI GCGC 1 cut(s) 621
Ksp22I TGATCA 1 cut(s) 1126
KspAI GTTAAC 1 cut(s) 40
KspI CCGCGG 1 cut(s) 249
Kzo9I GATC 2 cut(s) 921, 1126
LmnI GCTCC 1 cut(s) 418
Lsp1109I GCAGC 4 cut(s) 110, 113, 382, 707
LweI GCATC 3 cut(s) 645, 693, 1239
MaeI CTAG 1 cut(s) 1261
MaeIII GTNAC 2 cut(s) 566, 1256
MalI GATC 2 cut(s) 923, 1128
MboI GATC 2 cut(s) 921, 1126
MboII GAAGA 7 cut(s) 184, 187, 319, 542, 563, 691, 729
MflI RGATCY 1 cut(s) 921
MlyI GAGTC 2 cut(s) 87, 1079
MroXI GAANNNNTTC 2 cut(s) 79, 1022
MseI TTAA 6 cut(s) 29, 39, 605, 749, 1028, 1200
MslI CAYNNNNRTG 1 cut(s) 645
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 4 cut(s) 159, 526, 1163, 1243
MspR9I CCNGG 3 cut(s) 436, 527, 1244
Mva1269I GAATGC 1 cut(s) 1022
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 1114
NciI CCSGG 2 cut(s) 527, 1244
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 899
NdeII GATC 2 cut(s) 921, 1126
NlaIII CATG 5 cut(s) 13, 241, 306, 448, 1182
NlaIV GGNNCC 2 cut(s) 414, 524
NmeAIII GCCGAG 1 cut(s) 389
NmuCI GTSAC 1 cut(s) 1256
NspI RCATGY 3 cut(s) 241, 448, 1182
NspV TTCGAA 1 cut(s) 404
PaeI GCATGC 1 cut(s) 1182
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PctI GAATGC 1 cut(s) 1022
PdmI GAANNNNTTC 2 cut(s) 79, 1022
PfeI GAWTC 6 cut(s) 80, 266, 370, 401, 506, 1159
PflMI CCANNNNNTGG 1 cut(s) 900
PfoI TCCNGGA 1 cut(s) 1242
PkrI GCNGC 7 cut(s) 100, 103, 247, 397, 697, 837, 1254
PleI GAGTC 2 cut(s) 87, 1078
PpsI GAGTC 2 cut(s) 87, 1078
PsiI TTATAA 2 cut(s) 987, 1139
Psp6I CCWGG 1 cut(s) 434
PspGI CCWGG 1 cut(s) 434
PspN4I GGNNCC 2 cut(s) 414, 524
PspPI GGNCC 3 cut(s) 315, 523, 903
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 1 cut(s) 921
RseI CAYNNNNRTG 1 cut(s) 645
SacII CCGCGG 1 cut(s) 249
SaqAI TTAA 6 cut(s) 29, 39, 605, 749, 1028, 1200
SatI GCNGC 7 cut(s) 99, 102, 246, 396, 696, 836, 1253
Sau3AI GATC 2 cut(s) 921, 1126
Sau96I GGNCC 3 cut(s) 315, 523, 903
SchI GAGTC 2 cut(s) 87, 1079
ScrFI CCNGG 3 cut(s) 436, 527, 1244
SfaNI GCATC 3 cut(s) 645, 693, 1239
Sfr303I CCGCGG 1 cut(s) 249
SfuI TTCGAA 1 cut(s) 404
SgrBI CCGCGG 1 cut(s) 249
SinI GGWCC 2 cut(s) 523, 903
SmiMI CAYNNNNRTG 1 cut(s) 645
SmlI CTYRAG 1 cut(s) 776
SmoI CTYRAG 1 cut(s) 776
SphI GCATGC 1 cut(s) 1182
SsiI CCGC 5 cut(s) 246, 248, 398, 599, 835
SspI AATATT 1 cut(s) 1003
SspMI CTAG 1 cut(s) 1261
StyD4I CCNGG 3 cut(s) 434, 525, 1242
StyI CCWWGG 2 cut(s) 9, 624
TaaI ACNGT 2 cut(s) 557, 685
TaqI TCGA 4 cut(s) 89, 118, 198, 404
TauI GCSGC 2 cut(s) 248, 838
TfiI GAWTC 6 cut(s) 80, 266, 370, 401, 506, 1159
Tru1I TTAA 6 cut(s) 29, 39, 605, 749, 1028, 1200
Tru9I TTAA 6 cut(s) 29, 39, 605, 749, 1028, 1200
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 1256
TseI GCWGC 5 cut(s) 98, 101, 395, 695, 1252
Tsp45I GTSAC 1 cut(s) 1256
TspDTI ATGAA 3 cut(s) 759, 876, 922
TspGWI ACGGA 4 cut(s) 145, 187, 405, 554
TspRI CASTG 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 900
VpaK11BI GGWCC 2 cut(s) 523, 903
XapI RAATTY 4 cut(s) 293, 590, 974, 1095
XceI RCATGY 3 cut(s) 241, 448, 1182
XmnI GAANNNNTTC 2 cut(s) 79, 1022
XspI CTAG 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.