Rw4G000770

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004997
Physical Location & Seq
Reverse (-)
1533193 .. 1538834
5642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G000770.1

Sequence Viewer

Length: 1260 bp
ATGTTGTCCATGGTCTTATGCGAGAACTTCGTCTTTGTTAATAACCACAACAAGCAGACTTTGTTTTCGCACAAAATTGATTCGCAGTCGAGGCTCGCAGCAGCAGCAGTTGAGGTGGGTCGAGGAGGAGGAGGACGGAGGTGCAGTGGGTTCGTTGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCGGAGGAGGAGGAGGAGGAGAACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGGGCCGAAGCAATCGTGGATTTGAGGGAAGCACAGCAGGATATGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCGAACTCGTCCTCGTCCTGGGAATACTTGCAGTCTGATATTCCTACCGAAACTGAAGCCGAAAAGGGGGTGCTTTACTCCCTCACCGATTCCCTTCTTGGAAGGGAAGATGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCCCTCAATTCGGCTAAATTTTTGGCGGTTTTAATAATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCAGCAGAGATACTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATGGAGAGAGCCAGATTTCAGCTTGAGATGGAAATTGGTAAAGCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTCATTTGGGATCTCATTGTTCATTCTTTTATACTGCAACCAGAGTAAAGTAGCTTTGCTGAAATTTACAGGTTATAAGATAATAAATAATATTTCAGATACTGGGAAGGCATTTCTAATTATACTCATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAGACTTTGATAGAAATTTTTGTCGAGCATTATGGGCTTCAGGTTGATCAAGCAGTTATAACCATTTTTATCTGCTTGATTCCAGTTATTATTGACGCATGCGTGAAACTTTGGCTGTTTAAGTTTCTTCCGAGATTATCCCCGAAGGTGGCAAACATATTCCGGGAGATGCAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

419

Amino Acids

48.66

Weight (kDa)

4.91

Isoelectric Point (pI)

53.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 188 - 417 4.7e-76 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 984, 1136
Acc36I ACCTGC 1 cut(s) 546
AccB7I CCANNNNNTGG 1 cut(s) 897
AccII CGCG 1 cut(s) 254
AciI CCGC 5 cut(s) 252, 254, 413, 596, 832
AclWI GGATC 1 cut(s) 926
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 4 cut(s) 308, 587, 971, 1092
AcuI CTGAAG 2 cut(s) 492, 1100
AfiI CCNNNNNNNGG 5 cut(s) 435, 483, 580, 897, 1225
AgsI TTSAA 3 cut(s) 232, 731, 872
AjnI CCWGG 1 cut(s) 434
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 7 cut(s) 195, 302, 727, 772, 794, 850, 962
AluI AGCT 7 cut(s) 195, 302, 727, 772, 794, 850, 962
AlwI GGATC 1 cut(s) 926
AlwNI CAGNNNCTG 1 cut(s) 1010
AoxI GGCC 2 cut(s) 249, 330
ApeKI GCWGC 6 cut(s) 98, 101, 104, 410, 692, 1249
ApoI RAATTY 4 cut(s) 308, 587, 971, 1092
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
AspLEI GCGC 1 cut(s) 620
AspS9I GGNCC 2 cut(s) 330, 900
AsuC2I CCSGG 1 cut(s) 1241
AsuHPI GGTGA 1 cut(s) 493
AsuII TTCGAA 1 cut(s) 419
AvaII GGWCC 1 cut(s) 900
BbsI GAAGAC 3 cut(s) 329, 555, 683
BbvI GCAGC 5 cut(s) 110, 113, 116, 397, 704
BccI CCATC 1 cut(s) 772
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 1 cut(s) 436
BclI TGATCA 1 cut(s) 1123
BcnI CCSGG 1 cut(s) 1241
BfaI CTAG 1 cut(s) 1258
BfuAI ACCTGC 1 cut(s) 546
BisI GCNGC 8 cut(s) 99, 102, 105, 252, 411, 693, 833, 1250
BlsI GCNGC 8 cut(s) 100, 103, 106, 253, 412, 694, 834, 1251
Bme1390I CCNGG 2 cut(s) 436, 1241
Bme18I GGWCC 1 cut(s) 900
BmgT120I GGNCC 2 cut(s) 330, 900
BmrFI CCNGG 2 cut(s) 436, 1241
BmrI ACTGGG 1 cut(s) 1020
BmsI GCATC 2 cut(s) 642, 1236
BmuI ACTGGG 1 cut(s) 1020
BpiI GAAGAC 3 cut(s) 329, 555, 683
Bpu14I TTCGAA 1 cut(s) 419
BpuEI CTTGAG 1 cut(s) 794
BpuMI CCSGG 1 cut(s) 1241
BsaJI CCNNGG 4 cut(s) 9, 252, 435, 621
BsaXI ACNNNNNCTCC 4 cut(s) 130, 160, 806, 836
Bsc4I CCNNNNNNNGG 5 cut(s) 435, 483, 580, 897, 1225
Bse1I ACTGG 3 cut(s) 708, 1015, 1160
BseBI CCWGG 1 cut(s) 436
BseDI CCNNGG 4 cut(s) 9, 252, 435, 621
BseGI GGATG 1 cut(s) 712
BseLI CCNNNNNNNGG 5 cut(s) 435, 483, 580, 897, 1225
BseMII CTCAG 3 cut(s) 294, 842, 1056
BseNI ACTGG 3 cut(s) 708, 1015, 1160
BseXI GCAGC 5 cut(s) 110, 113, 116, 397, 704
BsgI GTGCAG 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 2 cut(s) 251, 332
BsiSI CCGG 2 cut(s) 165, 1240
BslI CCNNNNNNNGG 5 cut(s) 435, 483, 580, 897, 1225
BsnI GGCC 2 cut(s) 251, 332
Bsp119I TTCGAA 1 cut(s) 419
Bsp143I GATC 2 cut(s) 918, 1123
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 5 cut(s) 252, 254, 413, 596, 832
BspANI GGCC 2 cut(s) 251, 332
BspCNI CTCAG 3 cut(s) 295, 843, 1057
BspFNI CGCG 1 cut(s) 254
BspMI ACCTGC 1 cut(s) 546
BspPI GGATC 1 cut(s) 926
BspT104I TTCGAA 1 cut(s) 419
BsrI ACTGG 3 cut(s) 708, 1015, 1160
BssECI CCNNGG 4 cut(s) 9, 252, 435, 621
BssMI GATC 2 cut(s) 918, 1123
BssT1I CCWWGG 2 cut(s) 9, 621
Bst2UI CCWGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 554, 682
BstBI TTCGAA 1 cut(s) 419
BstC8I GCNNGC 4 cut(s) 96, 249, 408, 1177
BstDEI CTNAG 3 cut(s) 303, 851, 1065
BstDSI CCRYGG 2 cut(s) 9, 252
BstF5I GGATG 1 cut(s) 712
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 620
BstKTI GATC 2 cut(s) 921, 1126
BstMBI GATC 2 cut(s) 918, 1123
BstMWI GCNNNNNNNGC 3 cut(s) 91, 104, 1111
BstNI CCWGG 1 cut(s) 436
BstNSI RCATGY 2 cut(s) 247, 1179
BstSCI CCNGG 2 cut(s) 434, 1239
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 5 cut(s) 110, 113, 116, 397, 704
BstV2I GAAGAC 3 cut(s) 329, 555, 683
BstX2I RGATCY 1 cut(s) 918
BstYI RGATCY 1 cut(s) 918
BsuRI GGCC 2 cut(s) 251, 332
BtgI CCRYGG 2 cut(s) 9, 252
BtsCI GGATG 1 cut(s) 712
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 151
BveI ACCTGC 1 cut(s) 546
Cac8I GCNNGC 4 cut(s) 96, 249, 408, 1177
CaiI CAGNNNCTG 1 cut(s) 1010
CfoI GCGC 1 cut(s) 620
Cfr13I GGNCC 2 cut(s) 330, 900
Cfr42I CCGCGG 1 cut(s) 255
CseI GACGC 2 cut(s) 1181, 1241
CviAII CATG 3 cut(s) 10, 244, 1176
DdeI CTNAG 3 cut(s) 303, 851, 1065
DpnI GATC 2 cut(s) 920, 1125
DpnII GATC 2 cut(s) 918, 1123
EaeI YGGCCR 1 cut(s) 249
Eco130I CCWWGG 2 cut(s) 9, 621
Eco47I GGWCC 1 cut(s) 900
Eco57I CTGAAG 2 cut(s) 492, 1100
EcoRII CCWGG 1 cut(s) 434
EcoT14I CCWWGG 2 cut(s) 9, 621
ErhI CCWWGG 2 cut(s) 9, 621
FaeI CATG 3 cut(s) 13, 247, 1179
FatI CATG 3 cut(s) 9, 243, 1175
FauNDI CATATG 1 cut(s) 896
FbaI TGATCA 1 cut(s) 1123
Fnu4HI GCNGC 8 cut(s) 99, 102, 105, 252, 411, 693, 833, 1250
FokI GGATG 1 cut(s) 719
Fsp4HI GCNGC 8 cut(s) 99, 102, 105, 252, 411, 693, 833, 1250
FspBI CTAG 1 cut(s) 1258
GlaI GCGC 1 cut(s) 619
GluI GCNGC 8 cut(s) 99, 102, 105, 252, 411, 693, 833, 1250
HaeIII GGCC 2 cut(s) 251, 332
HapII CCGG 2 cut(s) 165, 1240
HgaI GACGC 2 cut(s) 1181, 1241
HhaI GCGC 1 cut(s) 620
Hin1II CATG 3 cut(s) 13, 247, 1179
Hin6I GCGC 1 cut(s) 618
HinP1I GCGC 1 cut(s) 618
HindIII AAGCTT 1 cut(s) 725
HinfI GANTC 7 cut(s) 80, 281, 385, 416, 506, 1067, 1156
HpaII CCGG 2 cut(s) 165, 1240
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 1 cut(s) 637
Hpy188I TCNGA 8 cut(s) 298, 454, 713, 808, 904, 1006, 1066, 1209
Hpy188III TCNNGA 2 cut(s) 638, 662
Hpy8I GTNNAC 1 cut(s) 637
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 5 cut(s) 206, 513, 521, 1009, 1216
HpyCH4III ACNGT 2 cut(s) 554, 682
HpyCH4V TGCA 5 cut(s) 144, 448, 692, 945, 1249
HpyF10VI GCNNNNNNNGC 3 cut(s) 91, 104, 1111
HpyF3I CTNAG 3 cut(s) 303, 851, 1065
Hsp92II CATG 3 cut(s) 13, 247, 1179
HspAI GCGC 1 cut(s) 618
Ksp22I TGATCA 1 cut(s) 1123
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 2 cut(s) 918, 1123
LmnI GCTCC 1 cut(s) 799
Lsp1109I GCAGC 5 cut(s) 110, 113, 116, 397, 704
LweI GCATC 2 cut(s) 642, 1236
MaeI CTAG 1 cut(s) 1258
MaeIII GTNAC 2 cut(s) 563, 1253
MalI GATC 2 cut(s) 920, 1125
MboI GATC 2 cut(s) 918, 1123
MboII GAAGA 8 cut(s) 190, 193, 334, 536, 560, 688, 726, 1196
MflI RGATCY 1 cut(s) 918
MlyI GAGTC 1 cut(s) 1076
MseI TTAA 4 cut(s) 39, 602, 746, 1197
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 2 cut(s) 165, 1240
MspR9I CCNGG 2 cut(s) 436, 1241
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 3 cut(s) 91, 104, 1111
NciI CCSGG 1 cut(s) 1241
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 896
NdeII GATC 2 cut(s) 918, 1123
NlaIII CATG 3 cut(s) 13, 247, 1179
NmuCI GTSAC 1 cut(s) 1253
NspI RCATGY 2 cut(s) 247, 1179
NspV TTCGAA 1 cut(s) 419
PaeI GCATGC 1 cut(s) 1179
PfeI GAWTC 6 cut(s) 80, 281, 385, 416, 506, 1156
PflMI CCANNNNNTGG 1 cut(s) 897
PfoI TCCNGGA 1 cut(s) 1239
PkrI GCNGC 8 cut(s) 100, 103, 106, 253, 412, 694, 834, 1251
PleI GAGTC 1 cut(s) 1075
PpsI GAGTC 1 cut(s) 1075
PsiI TTATAA 2 cut(s) 984, 1136
Psp6I CCWGG 1 cut(s) 434
PspGI CCWGG 1 cut(s) 434
PspPI GGNCC 2 cut(s) 330, 900
PstNI CAGNNNCTG 1 cut(s) 1010
PsuI RGATCY 1 cut(s) 918
RseI CAYNNNNRTG 1 cut(s) 642
SacII CCGCGG 1 cut(s) 255
SaqAI TTAA 4 cut(s) 39, 602, 746, 1197
SatI GCNGC 8 cut(s) 99, 102, 105, 252, 411, 693, 833, 1250
Sau3AI GATC 2 cut(s) 918, 1123
Sau96I GGNCC 2 cut(s) 330, 900
SchI GAGTC 1 cut(s) 1076
ScrFI CCNGG 2 cut(s) 436, 1241
SfaNI GCATC 2 cut(s) 642, 1236
Sfr303I CCGCGG 1 cut(s) 255
SfuI TTCGAA 1 cut(s) 419
SgrBI CCGCGG 1 cut(s) 255
SinI GGWCC 1 cut(s) 900
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
SphI GCATGC 1 cut(s) 1179
SsiI CCGC 5 cut(s) 252, 254, 413, 596, 832
SspI AATATT 1 cut(s) 1000
SspMI CTAG 1 cut(s) 1258
StyD4I CCNGG 2 cut(s) 434, 1239
StyI CCWWGG 2 cut(s) 9, 621
TaaI ACNGT 2 cut(s) 554, 682
TaqI TCGA 5 cut(s) 89, 121, 204, 419, 1101
TauI GCSGC 2 cut(s) 254, 835
TfiI GAWTC 6 cut(s) 80, 281, 385, 416, 506, 1156
Tru1I TTAA 4 cut(s) 39, 602, 746, 1197
Tru9I TTAA 4 cut(s) 39, 602, 746, 1197
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 1253
TseI GCWGC 6 cut(s) 98, 101, 104, 410, 692, 1249
Tsp45I GTSAC 1 cut(s) 1253
TspDTI ATGAA 3 cut(s) 756, 873, 919
TspGWI ACGGA 3 cut(s) 151, 193, 551
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 897
VpaK11BI GGWCC 1 cut(s) 900
XapI RAATTY 4 cut(s) 308, 587, 971, 1092
XceI RCATGY 2 cut(s) 247, 1179
XspI CTAG 1 cut(s) 1258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.