MD10G1290500.v1.1

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004997
Physical Location & Seq
Reverse (-)
37917031 .. 37923108
6078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1290500.v1.1.491

Sequence Viewer

Length: 1308 bp
ATGTTAATCATGTCCTCCTCCGCCGTCTTGTGCAAAAGCTTGATCTTTGTTAACCCCAAGCTCAATCTGCTCAGCCGCAACTCCATTTTCCACGGAAATTTGTCGACGCCACTGCCTCTGGTGGTTGATACAAGGCGGCGGAGGTCTGCCGCTGCAGAGCACATTCCAAATGCTTCTGGAAAAAGGAAAAGGAAAAGGACTTGGTGGCAAACATTCTTTTTCGACGACGATGGGAATTGGCTTGGTTTGAAGGAGGACGACATGCTGGCGGCGGAGGAGGAGCAGGAAGAGGAGTTAGATGGCTCCTCCCCAGCTGCCGCCAACAGCAGATGCGAAGAAGCTGAGAAATTTGATGCGTGGAAGACGAGGGCAGAAGCAATCGTGGAGTTGAGGGAAGCACAGCAGGATATGACCAACGAGGAGAACCGCATGTGGGAGGATTGGATTTCGGATTCTAATTCTAACTCTAATTCTTCTACTTCCTCCTGGGAAGCATCTGTGCAGTCAGATCCTGCTCAAGAAAGGGGATTGATTTACTCTCTCAGCGATTTTGTCCTCGGAAAGGAAGAAGATGAGGAGGATGACATGCTCTATGAAGACCGCGTTTTTCGCTACGCCTCACTCAATTCGGCTAAATTTTTGGCAGTATTAATTATCATACCCTGGGCCTTGGATTTTGTGGTTCACGACTATGTTCTGATGCCTTTCTTAGACAGATATGTGAAGACCGTACCACTTGCAGCACAGTTGCTTGACGTCAGAAGAAATCAAAAGCTTCAAATGGTTGAGGAATTAAAAATGGAGGGAGCAAGATTTCATCTTGAGATGGAAATTGGAAAATCTCCACCGCTTTCTGATGATGAGGTTTGGTGGGAGTTGCGGCATAAAGCGTTAGAGCTGAGAGATGAATGGAGACTAGAGAATCGTAAAGCATTTGCCAACATATGGTCAGATATGATATTCGGGATCTCATTATTCGTTCTTTTATACAGCAATCAGAGTAAAGTAGCATTGCTGAAATTTACAGGTTATAAGATAATAAATAATATTTCAGATACCGGGAAGGCATTTCTAATTATACTTATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAAACGTTGTTAGAAATTTTTGTTGAGCATTATGGGCTTGAGGTTGATCAGTCTGCCATAACCATTTTCATCTGCCTGATTCCAGTTATCATGGATGCATGCGTCAAACTTTGGCTGTTTAAATTCCTTCCAAGATTATCCCCGAAGGTGACAAACATATTCCGGGAAATGAAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

436

Amino Acids

50.4

Weight (kDa)

5.03

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 204 - 433 3.2e-77 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1032
AatII GACGTC 1 cut(s) 759
AccB7I CCANNNNNTGG 1 cut(s) 945
AccI GTMKAC 1 cut(s) 104
AccII CGCG 1 cut(s) 603
AclI AACGTT 1 cut(s) 1130
AclWI GGATC 2 cut(s) 503, 974
AcsI RAATTY 6 cut(s) 97, 347, 635, 1019, 1140, 1247
AcyI GRCGYC 2 cut(s) 107, 756
AfaI GTAC 1 cut(s) 732
AfiI CCNNNNNNNGG 3 cut(s) 433, 562, 945
AgsI TTSAA 2 cut(s) 250, 779
AjnI CCWGG 2 cut(s) 485, 662
AluBI AGCT 6 cut(s) 39, 61, 314, 341, 775, 898
AluI AGCT 6 cut(s) 39, 61, 314, 341, 775, 898
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 907
AlwI GGATC 2 cut(s) 503, 974
AlwNI CAGNNNCTG 1 cut(s) 512
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 3 cut(s) 152, 314, 740
ApoI RAATTY 6 cut(s) 97, 347, 635, 1019, 1140, 1247
AseI ATTAAT 1 cut(s) 650
AspS9I GGNCC 1 cut(s) 666
AsuC2I CCSGG 2 cut(s) 1060, 1289
AsuHPI GGTGA 1 cut(s) 1285
BbsI GAAGAC 3 cut(s) 368, 603, 731
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 3 cut(s) 139, 301, 752
BccI CCATC 3 cut(s) 224, 293, 820
BceAI ACGGC 1 cut(s) 8
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 2 cut(s) 487, 664
BclI TGATCA 1 cut(s) 1171
BcnI CCSGG 2 cut(s) 1060, 1289
BcoDI GTCTC 1 cut(s) 907
BfaI CTAG 2 cut(s) 917, 1306
BfmI CTRYAG 1 cut(s) 153
BisI GCNGC 9 cut(s) 76, 137, 150, 153, 270, 315, 318, 741, 881
BlpI GCTNAGC 1 cut(s) 71
BlsI GCNGC 9 cut(s) 77, 138, 151, 154, 271, 316, 319, 742, 882
Bme1390I CCNGG 4 cut(s) 487, 664, 1060, 1289
BmgT120I GGNCC 1 cut(s) 666
BmiI GGNNCC 1 cut(s) 304
BmrFI CCNGG 4 cut(s) 487, 664, 1060, 1289
BmsI GCATC 5 cut(s) 320, 343, 503, 690, 1210
BpiI GAAGAC 3 cut(s) 368, 603, 731
Bpu1102I GCTNAGC 1 cut(s) 71
BpuEI CTTGAG 3 cut(s) 501, 842, 1184
BpuMI CCSGG 2 cut(s) 1060, 1289
BsaHI GRCGYC 2 cut(s) 107, 756
BsaJI CCNNGG 6 cut(s) 91, 486, 556, 662, 663, 669
Bsc4I CCNNNNNNNGG 3 cut(s) 433, 562, 945
Bse1I ACTGG 1 cut(s) 1208
Bse3DI GCAATG 1 cut(s) 1010
BseBI CCWGG 2 cut(s) 487, 664
BseDI CCNNGG 6 cut(s) 91, 486, 556, 662, 663, 669
BseGI GGATG 2 cut(s) 586, 1225
BseLI CCNNNNNNNGG 3 cut(s) 433, 562, 945
BseMI GCAATG 1 cut(s) 1010
BseMII CTCAG 5 cut(s) 85, 333, 556, 890, 1104
BseNI ACTGG 1 cut(s) 1208
BseRI GAGGAG 7 cut(s) 7, 290, 293, 295, 305, 434, 590
BseXI GCAGC 3 cut(s) 139, 301, 752
BseYI CCCAGC 1 cut(s) 310
BsgI GTGCAG 1 cut(s) 521
Bsh1236I CGCG 1 cut(s) 603
BshFI GGCC 1 cut(s) 668
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 2 cut(s) 1059, 1288
BslI CCNNNNNNNGG 3 cut(s) 433, 562, 945
BsmAI GTCTC 1 cut(s) 907
BsnI GGCC 1 cut(s) 668
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 4 cut(s) 42, 508, 966, 1171
Bsp1720I GCTNAGC 1 cut(s) 71
BspANI GGCC 1 cut(s) 668
BspCNI CTCAG 5 cut(s) 84, 334, 555, 891, 1105
BspFNI CGCG 1 cut(s) 603
BspLI GGNNCC 1 cut(s) 304
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 2 cut(s) 503, 974
BsrDI GCAATG 1 cut(s) 1010
BsrI ACTGG 1 cut(s) 1208
BssECI CCNNGG 6 cut(s) 91, 486, 556, 662, 663, 669
BssMI GATC 4 cut(s) 42, 508, 966, 1171
BssNI GRCGYC 2 cut(s) 107, 756
BssT1I CCWWGG 1 cut(s) 669
Bst2UI CCWGG 2 cut(s) 487, 664
Bst4CI ACNGT 2 cut(s) 730, 747
Bst6I CTCTTC 1 cut(s) 282
BstACI GRCGYC 2 cut(s) 107, 756
BstC8I GCNNGC 2 cut(s) 267, 1225
BstDEI CTNAG 6 cut(s) 71, 342, 542, 709, 899, 1113
BstDSI CCRYGG 1 cut(s) 91
BstENI CCTNNNNNAGG 1 cut(s) 560
BstF5I GGATG 2 cut(s) 586, 1225
BstFNI CGCG 1 cut(s) 603
BstKTI GATC 4 cut(s) 45, 511, 969, 1174
BstMAI GTCTC 1 cut(s) 907
BstMBI GATC 4 cut(s) 42, 508, 966, 1171
BstMWI GCNNNNNNNGC 3 cut(s) 67, 609, 1159
BstNI CCWGG 2 cut(s) 487, 664
BstNSI RCATGY 4 cut(s) 265, 433, 589, 1227
BstSCI CCNGG 4 cut(s) 485, 662, 1058, 1287
BstSFI CTRYAG 1 cut(s) 153
BstUI CGCG 1 cut(s) 603
BstV1I GCAGC 3 cut(s) 139, 301, 752
BstV2I GAAGAC 3 cut(s) 368, 603, 731
BstX2I RGATCY 2 cut(s) 508, 966
BstYI RGATCY 2 cut(s) 508, 966
BsuRI GGCC 1 cut(s) 668
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 2 cut(s) 586, 1225
BtsI GCAGTG 1 cut(s) 110
BtsIMutI CAGTG 1 cut(s) 110
Cac8I GCNNGC 2 cut(s) 267, 1225
CaiI CAGNNNCTG 1 cut(s) 512
Cfr13I GGNCC 1 cut(s) 666
CseI GACGC 3 cut(s) 115, 1216, 1289
Csp6I GTAC 1 cut(s) 731
CviAII CATG 6 cut(s) 10, 262, 430, 586, 1216, 1224
CviQI GTAC 1 cut(s) 731
DdeI CTNAG 6 cut(s) 71, 342, 542, 709, 899, 1113
DpnI GATC 4 cut(s) 44, 510, 968, 1173
DpnII GATC 4 cut(s) 42, 508, 966, 1171
DraI TTTAAA 1 cut(s) 1246
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
EciI GGCGGA 3 cut(s) 10, 154, 287
Eco130I CCWWGG 1 cut(s) 669
EcoNI CCTNNNNNAGG 1 cut(s) 560
EcoRII CCWGG 2 cut(s) 485, 662
EcoT14I CCWWGG 1 cut(s) 669
EcoT22I ATGCAT 1 cut(s) 1225
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 6 cut(s) 13, 265, 433, 589, 1219, 1227
FatI CATG 6 cut(s) 9, 261, 429, 585, 1215, 1223
FauNDI CATATG 1 cut(s) 944
FbaI TGATCA 1 cut(s) 1171
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 9 cut(s) 76, 137, 150, 153, 270, 315, 318, 741, 881
FokI GGATG 2 cut(s) 593, 1232
Fsp4HI GCNGC 9 cut(s) 76, 137, 150, 153, 270, 315, 318, 741, 881
FspBI CTAG 2 cut(s) 917, 1306
GluI GCNGC 9 cut(s) 76, 137, 150, 153, 270, 315, 318, 741, 881
GsaI CCCAGC 1 cut(s) 314
HaeIII GGCC 1 cut(s) 668
HapII CCGG 2 cut(s) 1059, 1288
HgaI GACGC 3 cut(s) 115, 1216, 1289
Hin1I GRCGYC 2 cut(s) 107, 756
Hin1II CATG 6 cut(s) 13, 265, 433, 589, 1219, 1227
HincII GTYRAC 2 cut(s) 52, 105
HindII GTYRAC 2 cut(s) 52, 105
HindIII AAGCTT 2 cut(s) 37, 773
HinfI GANTC 4 cut(s) 452, 922, 1115, 1204
HpaI GTTAAC 1 cut(s) 52
HpaII CCGG 2 cut(s) 1059, 1288
HphI GGTGA 1 cut(s) 1285
Hpy166II GTNNAC 3 cut(s) 52, 105, 685
Hpy188III TCNNGA 5 cut(s) 177, 518, 686, 821, 964
Hpy8I GTNNAC 3 cut(s) 52, 105, 685
Hpy99I CGWCG 3 cut(s) 109, 227, 230
HpyAV CCTTC 4 cut(s) 244, 1057, 1262, 1264
HpyCH4III ACNGT 2 cut(s) 730, 747
HpyCH4IV ACGT 2 cut(s) 756, 1130
HpyCH4V TGCA 5 cut(s) 33, 155, 502, 740, 1223
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 609, 1159
HpyF3I CTNAG 6 cut(s) 71, 342, 542, 709, 899, 1113
HpySE526I ACGT 2 cut(s) 756, 1130
Hsp92I GRCGYC 2 cut(s) 107, 756
Hsp92II CATG 6 cut(s) 13, 265, 433, 589, 1219, 1227
Ksp22I TGATCA 1 cut(s) 1171
KspAI GTTAAC 1 cut(s) 52
Kzo9I GATC 4 cut(s) 42, 508, 966, 1171
LmnI GCTCC 3 cut(s) 280, 308, 806
Lsp1109I GCAGC 3 cut(s) 139, 301, 752
LweI GCATC 5 cut(s) 320, 343, 503, 690, 1210
MaeI CTAG 2 cut(s) 917, 1306
MaeII ACGT 2 cut(s) 756, 1130
MaeIII GTNAC 2 cut(s) 1273, 1301
MalI GATC 4 cut(s) 44, 510, 968, 1173
MboI GATC 4 cut(s) 42, 508, 966, 1171
MboII GAAGA 9 cut(s) 299, 347, 373, 465, 578, 581, 608, 736, 774
MflI RGATCY 2 cut(s) 508, 966
MhlI GDGCHC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 1124
Mph1103I ATGCAT 1 cut(s) 1225
MseI TTAA 5 cut(s) 5, 51, 650, 794, 1245
MslI CAYNNNNRTG 1 cut(s) 690
MspA1I CMGCKG 2 cut(s) 152, 314
MspI CCGG 2 cut(s) 1059, 1288
MspR9I CCNGG 4 cut(s) 487, 664, 1060, 1289
MvaI CCWGG 2 cut(s) 487, 664
MvnI CGCG 1 cut(s) 603
MwoI GCNNNNNNNGC 3 cut(s) 67, 609, 1159
NciI CCSGG 2 cut(s) 1060, 1289
NdeI CATATG 1 cut(s) 944
NdeII GATC 4 cut(s) 42, 508, 966, 1171
NlaIII CATG 6 cut(s) 13, 265, 433, 589, 1219, 1227
NlaIV GGNNCC 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 1273, 1301
NsiI ATGCAT 1 cut(s) 1225
NspI RCATGY 4 cut(s) 265, 433, 589, 1227
PaeI GCATGC 1 cut(s) 1227
PasI CCCWGGG 1 cut(s) 663
PfeI GAWTC 3 cut(s) 452, 922, 1204
PflMI CCANNNNNTGG 1 cut(s) 945
PfoI TCCNGGA 1 cut(s) 1287
PkrI GCNGC 9 cut(s) 77, 138, 151, 154, 271, 316, 319, 742, 882
PleI GAGTC 1 cut(s) 1123
PpsI GAGTC 1 cut(s) 1123
PshBI ATTAAT 1 cut(s) 650
PsiI TTATAA 1 cut(s) 1032
Psp1406I AACGTT 1 cut(s) 1130
Psp6I CCWGG 2 cut(s) 485, 662
PspFI CCCAGC 1 cut(s) 310
PspGI CCWGG 2 cut(s) 485, 662
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 1 cut(s) 666
PstI CTGCAG 1 cut(s) 157
PstNI CAGNNNCTG 1 cut(s) 512
PsuI RGATCY 2 cut(s) 508, 966
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 1 cut(s) 732
RsaNI GTAC 1 cut(s) 731
RseI CAYNNNNRTG 1 cut(s) 690
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 5 cut(s) 5, 51, 650, 794, 1245
SatI GCNGC 9 cut(s) 76, 137, 150, 153, 270, 315, 318, 741, 881
Sau3AI GATC 4 cut(s) 42, 508, 966, 1171
Sau96I GGNCC 1 cut(s) 666
SchI GAGTC 1 cut(s) 1124
ScrFI CCNGG 4 cut(s) 487, 664, 1060, 1289
SduI GDGCHC 1 cut(s) 162
SfaNI GCATC 5 cut(s) 320, 343, 503, 690, 1210
SfcI CTRYAG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 690
SmlI CTYRAG 3 cut(s) 516, 821, 1163
SmoI CTYRAG 3 cut(s) 516, 821, 1163
SphI GCATGC 1 cut(s) 1227
SspI AATATT 1 cut(s) 1048
SspMI CTAG 2 cut(s) 917, 1306
StyD4I CCNGG 4 cut(s) 485, 662, 1058, 1287
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 2 cut(s) 730, 747
TaiI ACGT 2 cut(s) 759, 1133
TaqI TCGA 2 cut(s) 104, 222
TauI GCSGC 6 cut(s) 78, 139, 152, 272, 320, 883
TfiI GAWTC 3 cut(s) 452, 922, 1204
Tru1I TTAA 5 cut(s) 5, 51, 650, 794, 1245
Tru9I TTAA 5 cut(s) 5, 51, 650, 794, 1245
TscAI CASTG 1 cut(s) 117
TseFI GTSAC 2 cut(s) 1273, 1301
TseI GCWGC 3 cut(s) 152, 314, 740
Tsp45I GTSAC 2 cut(s) 1273, 1301
TspDTI ATGAA 4 cut(s) 609, 806, 921, 1183
TspGWI ACGGA 1 cut(s) 108
TspRI CASTG 1 cut(s) 117
Van91I CCANNNNNTGG 1 cut(s) 945
VspI ATTAAT 1 cut(s) 650
XagI CCTNNNNNAGG 1 cut(s) 560
XapI RAATTY 6 cut(s) 97, 347, 635, 1019, 1140, 1247
XceI RCATGY 4 cut(s) 265, 433, 589, 1227
XmiI GTMKAC 1 cut(s) 104
XspI CTAG 2 cut(s) 917, 1306
ZraI GACGTC 1 cut(s) 757
Zsp2I ATGCAT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.