Rroxscaffold_5G00332850

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004997
Physical Location & Seq
Forward (+)
209260 .. 214676
5417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00332850.1

Sequence Viewer

Length: 1272 bp
ATGTTGTCCATGGTCTTATGCGAGAACTTCATCTTTGTTAACAACCACAACAAGCAGACTTTGTTTTCGCACAAAATTGATTCGCAGTCGAGACTCGCAGCAGCAGCAGCACTTGAGGTGGGTCGAGGAGGAGGACGGAGGTGCAGTGGGTTCGTTGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCGGAGGAGGAAGAGGAGGAGAACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGGGCCGAAGCAATCGTGGATTTGAGGGAAGCACAGCAGGATCTGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCCAACTCGTCTCCGTCGTCCTCGTCCTGGGAATACATGCAGTCTGATATTCCTACCGAAACTGAAGCCGAAAAGGGGGTGCTTTACTCTCTCACCGATTCCCTTCTTGGAAGGGACCCGGAAGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCACTCAATTCGGCTAAATTTTTGGCGGTTTTAATAATTATACCCTGCGCCTTGGATTTTGTGGTTCATGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCAGCAGAGATGCTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATTGAGAGAGCAAGATTTCAGCTTGAGATGGAAATTGGTAAAGCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTTATTTGGGATCTCATTATTCATTCTTTTATACTGCAACCAGAGTAAAGTAGCTTTGCTGAAATTTACAGGTTATAAGATAATAAATAATATTTCAGATACTGGGAAGGCATTTCTAATTATACTCATCACAGATATTTTTTTAGGGTATCATTCTGAGTCTGGTTGGCAGACTTTGATAGAAATTTTTGTTGAGCATTATGGGCTTCAGGTTGATCAATCAGTTATAACCATTTTTATCTGCTTGATTCCGGTTATTATTGACGCCTGCGTGAAACTTTGGCTGTTTAAGTTCCTTCCAAGATTATCCCCGAAGGTGGCAAACATATTCCGGGAGATGCAGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

423

Amino Acids

49.11

Weight (kDa)

4.91

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 192 - 421 9.9e-77 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 996, 1148
Acc36I ACCTGC 1 cut(s) 558
AccB7I CCANNNNNTGG 1 cut(s) 909
AccII CGCG 1 cut(s) 254
AciI CCGC 5 cut(s) 252, 254, 413, 608, 844
AclWI GGATC 2 cut(s) 375, 938
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 4 cut(s) 308, 599, 983, 1104
AcuI CTGAAG 2 cut(s) 501, 1112
AcyI GRCGYC 1 cut(s) 1185
AfiI CCNNNNNNNGG 4 cut(s) 444, 492, 909, 1237
AgsI TTSAA 3 cut(s) 232, 743, 884
AjnI CCWGG 1 cut(s) 443
AloI GAACNNNNNNTCC 2 cut(s) 266, 298
AluBI AGCT 7 cut(s) 195, 302, 739, 784, 806, 862, 974
AluI AGCT 7 cut(s) 195, 302, 739, 784, 806, 862, 974
Alw26I GTCTC 2 cut(s) 85, 432
AlwI GGATC 2 cut(s) 375, 938
AlwNI CAGNNNCTG 2 cut(s) 370, 1022
AoxI GGCC 2 cut(s) 249, 330
ApeKI GCWGC 7 cut(s) 98, 101, 104, 107, 410, 704, 1261
ApoI RAATTY 4 cut(s) 308, 599, 983, 1104
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 3 cut(s) 330, 532, 912
AsuC2I CCSGG 2 cut(s) 536, 1253
AsuHPI GGTGA 1 cut(s) 502
AvaII GGWCC 2 cut(s) 532, 912
BbsI GAAGAC 3 cut(s) 329, 567, 695
BbvI GCAGC 6 cut(s) 110, 113, 116, 119, 397, 716
BccI CCATC 1 cut(s) 784
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 1 cut(s) 445
BclI TGATCA 1 cut(s) 1135
BcnI CCSGG 2 cut(s) 536, 1253
BcoDI GTCTC 2 cut(s) 85, 432
BfaI CTAG 1 cut(s) 1270
BfuAI ACCTGC 1 cut(s) 558
BisI GCNGC 9 cut(s) 99, 102, 105, 108, 252, 411, 705, 845, 1262
BlsI GCNGC 9 cut(s) 100, 103, 106, 109, 253, 412, 706, 846, 1263
Bme1390I CCNGG 3 cut(s) 445, 536, 1253
Bme18I GGWCC 2 cut(s) 532, 912
BmgT120I GGNCC 3 cut(s) 330, 532, 912
BmiI GGNNCC 2 cut(s) 533, 534
BmrFI CCNGG 3 cut(s) 445, 536, 1253
BmrI ACTGGG 1 cut(s) 1032
BmsI GCATC 3 cut(s) 654, 702, 1248
BmuI ACTGGG 1 cut(s) 1032
BpiI GAAGAC 3 cut(s) 329, 567, 695
BpuEI CTTGAG 2 cut(s) 134, 806
BpuMI CCSGG 2 cut(s) 536, 1253
BsaHI GRCGYC 1 cut(s) 1185
BsaJI CCNNGG 4 cut(s) 9, 252, 444, 633
BsaWI WCCGGW 1 cut(s) 1171
BsaXI ACNNNNNCTCC 4 cut(s) 130, 160, 818, 848
Bsc4I CCNNNNNNNGG 4 cut(s) 444, 492, 909, 1237
Bse1I ACTGG 1 cut(s) 1027
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 4 cut(s) 9, 252, 444, 633
BseGI GGATG 1 cut(s) 724
BseLI CCNNNNNNNGG 4 cut(s) 444, 492, 909, 1237
BseMII CTCAG 4 cut(s) 294, 362, 854, 1068
BseNI ACTGG 1 cut(s) 1027
BseRI GAGGAG 7 cut(s) 141, 144, 272, 281, 284, 395, 839
BseXI GCAGC 6 cut(s) 110, 113, 116, 119, 397, 716
BsgI GTGCAG 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 2 cut(s) 251, 332
BsiSI CCGG 4 cut(s) 165, 536, 1172, 1252
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 4 cut(s) 444, 492, 909, 1237
BsmAI GTCTC 2 cut(s) 85, 432
BsmBI CGTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 2 cut(s) 251, 332
Bsp143I GATC 3 cut(s) 367, 930, 1135
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 5 cut(s) 252, 254, 413, 608, 844
BspANI GGCC 2 cut(s) 251, 332
BspCNI CTCAG 4 cut(s) 295, 363, 855, 1069
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 533, 534
BspMI ACCTGC 1 cut(s) 558
BspPI GGATC 2 cut(s) 375, 938
BsrI ACTGG 1 cut(s) 1027
BssECI CCNNGG 4 cut(s) 9, 252, 444, 633
BssMI GATC 3 cut(s) 367, 930, 1135
BssNI GRCGYC 1 cut(s) 1185
BssT1I CCWWGG 2 cut(s) 9, 633
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 2 cut(s) 566, 694
Bst6I CTCTTC 1 cut(s) 258
BstACI GRCGYC 1 cut(s) 1185
BstC8I GCNNGC 3 cut(s) 249, 408, 1189
BstDEI CTNAG 4 cut(s) 303, 371, 863, 1077
BstDSI CCRYGG 2 cut(s) 9, 252
BstF5I GGATG 1 cut(s) 724
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 3 cut(s) 370, 933, 1138
BstMAI GTCTC 2 cut(s) 85, 432
BstMBI GATC 3 cut(s) 367, 930, 1135
BstMWI GCNNNNNNNGC 3 cut(s) 104, 107, 1123
BstNI CCWGG 1 cut(s) 445
BstNSI RCATGY 2 cut(s) 247, 457
BstSCI CCNGG 3 cut(s) 443, 534, 1251
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 6 cut(s) 110, 113, 116, 119, 397, 716
BstV2I GAAGAC 3 cut(s) 329, 567, 695
BstX2I RGATCY 2 cut(s) 367, 930
BstYI RGATCY 2 cut(s) 367, 930
BsuRI GGCC 2 cut(s) 251, 332
BtgI CCRYGG 2 cut(s) 9, 252
BtsCI GGATG 1 cut(s) 724
BtsI GCAGTG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 151
BveI ACCTGC 1 cut(s) 558
Cac8I GCNNGC 3 cut(s) 249, 408, 1189
CaiI CAGNNNCTG 2 cut(s) 370, 1022
CciI TCATGA 1 cut(s) 649
CfoI GCGC 1 cut(s) 632
Cfr13I GGNCC 3 cut(s) 330, 532, 912
Cfr42I CCGCGG 1 cut(s) 255
CseI GACGC 2 cut(s) 1193, 1253
CviAII CATG 4 cut(s) 10, 244, 454, 650
DdeI CTNAG 4 cut(s) 303, 371, 863, 1077
DpnI GATC 3 cut(s) 369, 932, 1137
DpnII GATC 3 cut(s) 367, 930, 1135
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco130I CCWWGG 2 cut(s) 9, 633
Eco47I GGWCC 2 cut(s) 532, 912
Eco57I CTGAAG 2 cut(s) 501, 1112
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 443
EcoT14I CCWWGG 2 cut(s) 9, 633
ErhI CCWWGG 2 cut(s) 9, 633
Esp3I CGTCTC 1 cut(s) 432
FaeI CATG 4 cut(s) 13, 247, 457, 653
FaqI GGGAC 1 cut(s) 545
FatI CATG 4 cut(s) 9, 243, 453, 649
FauNDI CATATG 1 cut(s) 908
FbaI TGATCA 1 cut(s) 1135
Fnu4HI GCNGC 9 cut(s) 99, 102, 105, 108, 252, 411, 705, 845, 1262
FokI GGATG 1 cut(s) 731
Fsp4HI GCNGC 9 cut(s) 99, 102, 105, 108, 252, 411, 705, 845, 1262
FspBI CTAG 1 cut(s) 1270
GlaI GCGC 1 cut(s) 631
GluI GCNGC 9 cut(s) 99, 102, 105, 108, 252, 411, 705, 845, 1262
HaeIII GGCC 2 cut(s) 251, 332
HapII CCGG 4 cut(s) 165, 536, 1172, 1252
HgaI GACGC 2 cut(s) 1193, 1253
HhaI GCGC 1 cut(s) 632
Hin1I GRCGYC 1 cut(s) 1185
Hin1II CATG 4 cut(s) 13, 247, 457, 653
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 737
HinfI GANTC 8 cut(s) 80, 93, 281, 385, 416, 515, 1079, 1168
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 4 cut(s) 165, 536, 1172, 1252
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 8 cut(s) 298, 372, 463, 725, 820, 916, 1018, 1078
Hpy188III TCNNGA 3 cut(s) 90, 650, 674
Hpy8I GTNNAC 1 cut(s) 40
Hpy99I CGWCG 2 cut(s) 209, 436
HpyAV CCTTC 6 cut(s) 206, 522, 530, 1021, 1226, 1228
HpyCH4III ACNGT 2 cut(s) 566, 694
HpyCH4V TGCA 5 cut(s) 144, 457, 704, 957, 1261
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 107, 1123
HpyF3I CTNAG 4 cut(s) 303, 371, 863, 1077
Hsp92I GRCGYC 1 cut(s) 1185
Hsp92II CATG 4 cut(s) 13, 247, 457, 653
HspAI GCGC 1 cut(s) 630
KflI GGGWCCC 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 1135
KspAI GTTAAC 1 cut(s) 40
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 3 cut(s) 367, 930, 1135
LmnI GCTCC 1 cut(s) 811
Lsp1109I GCAGC 6 cut(s) 110, 113, 116, 119, 397, 716
LweI GCATC 3 cut(s) 654, 702, 1248
MaeI CTAG 1 cut(s) 1270
MaeIII GTNAC 2 cut(s) 575, 1265
MalI GATC 3 cut(s) 369, 932, 1137
MboI GATC 3 cut(s) 367, 930, 1135
MboII GAAGA 8 cut(s) 190, 193, 275, 334, 551, 572, 700, 738
MflI RGATCY 2 cut(s) 367, 930
MlyI GAGTC 2 cut(s) 87, 1088
MmeI TCCRAC 1 cut(s) 444
MseI TTAA 4 cut(s) 39, 614, 758, 1209
MslI CAYNNNNRTG 1 cut(s) 654
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 4 cut(s) 165, 536, 1172, 1252
MspR9I CCNGG 3 cut(s) 445, 536, 1253
MvaI CCWGG 1 cut(s) 445
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 3 cut(s) 104, 107, 1123
NciI CCSGG 2 cut(s) 536, 1253
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 908
NdeII GATC 3 cut(s) 367, 930, 1135
NlaIII CATG 4 cut(s) 13, 247, 457, 653
NlaIV GGNNCC 2 cut(s) 533, 534
NmuCI GTSAC 1 cut(s) 1265
NspI RCATGY 2 cut(s) 247, 457
PagI TCATGA 1 cut(s) 649
PcsI WCGNNNNNNNCGW 1 cut(s) 431
PfeI GAWTC 6 cut(s) 80, 281, 385, 416, 515, 1168
PflMI CCANNNNNTGG 1 cut(s) 909
PfoI TCCNGGA 1 cut(s) 1251
PkrI GCNGC 9 cut(s) 100, 103, 106, 109, 253, 412, 706, 846, 1263
PleI GAGTC 2 cut(s) 87, 1087
PpsI GAGTC 2 cut(s) 87, 1087
PpuMI RGGWCCY 1 cut(s) 532
PsiI TTATAA 2 cut(s) 996, 1148
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 443
PspGI CCWGG 1 cut(s) 443
PspN4I GGNNCC 2 cut(s) 533, 534
PspPI GGNCC 3 cut(s) 330, 532, 912
PspPPI RGGWCCY 1 cut(s) 532
PstNI CAGNNNCTG 2 cut(s) 370, 1022
PsuI RGATCY 2 cut(s) 367, 930
RseI CAYNNNNRTG 1 cut(s) 654
SacII CCGCGG 1 cut(s) 255
SaqAI TTAA 4 cut(s) 39, 614, 758, 1209
SatI GCNGC 9 cut(s) 99, 102, 105, 108, 252, 411, 705, 845, 1262
Sau3AI GATC 3 cut(s) 367, 930, 1135
Sau96I GGNCC 3 cut(s) 330, 532, 912
SchI GAGTC 2 cut(s) 87, 1088
ScrFI CCNGG 3 cut(s) 445, 536, 1253
SfaNI GCATC 3 cut(s) 654, 702, 1248
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SinI GGWCC 2 cut(s) 532, 912
SmiMI CAYNNNNRTG 1 cut(s) 654
SmlI CTYRAG 2 cut(s) 113, 785
SmoI CTYRAG 2 cut(s) 113, 785
SsiI CCGC 5 cut(s) 252, 254, 413, 608, 844
SspI AATATT 1 cut(s) 1012
SspMI CTAG 1 cut(s) 1270
StyD4I CCNGG 3 cut(s) 443, 534, 1251
StyI CCWWGG 2 cut(s) 9, 633
TaaI ACNGT 2 cut(s) 566, 694
TaqI TCGA 3 cut(s) 89, 124, 204
TauI GCSGC 2 cut(s) 254, 847
TfiI GAWTC 6 cut(s) 80, 281, 385, 416, 515, 1168
Tru1I TTAA 4 cut(s) 39, 614, 758, 1209
Tru9I TTAA 4 cut(s) 39, 614, 758, 1209
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 1265
TseI GCWGC 7 cut(s) 98, 101, 104, 107, 410, 704, 1261
Tsp45I GTSAC 1 cut(s) 1265
TspDTI ATGAA 5 cut(s) 19, 638, 768, 885, 931
TspGWI ACGGA 4 cut(s) 151, 193, 420, 563
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 909
VpaK11BI GGWCC 2 cut(s) 532, 912
XapI RAATTY 4 cut(s) 308, 599, 983, 1104
XceI RCATGY 2 cut(s) 247, 457
XspI CTAG 1 cut(s) 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.