Rh4CG001100

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004997
Physical Location & Seq
Forward (+)
227950 .. 234259
6310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG001100.1

Sequence Viewer

Length: 1008 bp
ATGTTGTCCATGGTCTTATGCGAGAACTTCATCTGTTTTAACAACCACAACAAGCAGACTTTGTTTTCGCACAAAATTGATTCGCAGTCGAGGCTCGCAGCAGCAGTTGAGGTGGGTCGAGGAGGAGGAGGACGGAGGTGCAGTGGGTTCGTTGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCGGAGGAGGAGGACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGGGCCGAAGCAATCGTGGATTTGAGGGAAGCACAGCAGGATATGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCCAACTCGTCCTCGTCCTGGGAATACATGCAGTCTGATATTCCTACCGAAACTGAAGCCGAAAAGGGGGTGCTTTACTCTCTCACCGATTCCCTTCTTGGAAGGGAAGATGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCCCTCAATTCGGCTAAATTTTTGGCGGTTTTAATAATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCGGCAGAGATGCTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATGGAGAGAGCAAGATTTCAGCTTGAGATGGAAATTGGTAAAGCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTCATTTGGGATCTCATTATTCATTCTTTTATACTGCAACCAGAGTAAAGTGAGTTATTATTACTTCTATTCTGTTCTTTCCTTTTATGTATTTAAATTGTCTTACCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

335

Amino Acids

39.28

Weight (kDa)

4.78

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 184 - 317 1.2e-26 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 534
AccB7I CCANNNNNTGG 1 cut(s) 885
AccII CGCG 1 cut(s) 251
AciI CCGC 6 cut(s) 249, 251, 401, 584, 680, 820
AclWI GGATC 1 cut(s) 914
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 2 cut(s) 296, 575
AcuI CTGAAG 1 cut(s) 480
AfiI CCNNNNNNNGG 4 cut(s) 423, 471, 568, 885
AgsI TTSAA 3 cut(s) 229, 719, 860
AjnI CCWGG 1 cut(s) 422
AluBI AGCT 6 cut(s) 192, 290, 715, 760, 782, 838
AluI AGCT 6 cut(s) 192, 290, 715, 760, 782, 838
AlwI GGATC 1 cut(s) 914
AoxI GGCC 2 cut(s) 246, 318
ApeKI GCWGC 3 cut(s) 98, 101, 398
ApoI RAATTY 2 cut(s) 296, 575
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
AspLEI GCGC 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 318, 888
AsuHPI GGTGA 1 cut(s) 481
AvaII GGWCC 1 cut(s) 888
BbsI GAAGAC 3 cut(s) 317, 543, 671
BbvI GCAGC 3 cut(s) 110, 113, 385
BccI CCATC 1 cut(s) 760
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 534
BisI GCNGC 6 cut(s) 99, 102, 249, 399, 681, 821
BlsI GCNGC 6 cut(s) 100, 103, 250, 400, 682, 822
Bme1390I CCNGG 1 cut(s) 424
Bme18I GGWCC 1 cut(s) 888
BmgT120I GGNCC 2 cut(s) 318, 888
BmrFI CCNGG 1 cut(s) 424
BmsI GCATC 2 cut(s) 630, 678
BpiI GAAGAC 3 cut(s) 317, 543, 671
BpuEI CTTGAG 1 cut(s) 782
BsaJI CCNNGG 4 cut(s) 9, 249, 423, 609
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 794, 824
Bsc4I CCNNNNNNNGG 4 cut(s) 423, 471, 568, 885
BseBI CCWGG 1 cut(s) 424
BseDI CCNNGG 4 cut(s) 9, 249, 423, 609
BseGI GGATG 1 cut(s) 700
BseLI CCNNNNNNNGG 4 cut(s) 423, 471, 568, 885
BseMII CTCAG 2 cut(s) 282, 830
BseRI GAGGAG 7 cut(s) 135, 138, 141, 269, 272, 383, 815
BseXI GCAGC 3 cut(s) 110, 113, 385
BsgI GTGCAG 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 2 cut(s) 248, 320
BsiSI CCGG 1 cut(s) 162
BslI CCNNNNNNNGG 4 cut(s) 423, 471, 568, 885
BsnI GGCC 2 cut(s) 248, 320
Bsp143I GATC 1 cut(s) 906
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 6 cut(s) 249, 251, 401, 584, 680, 820
BspANI GGCC 2 cut(s) 248, 320
BspCNI CTCAG 2 cut(s) 283, 831
BspFNI CGCG 1 cut(s) 251
BspMI ACCTGC 1 cut(s) 534
BspPI GGATC 1 cut(s) 914
BssECI CCNNGG 4 cut(s) 9, 249, 423, 609
BssMI GATC 1 cut(s) 906
BssT1I CCWWGG 2 cut(s) 9, 609
Bst2UI CCWGG 1 cut(s) 424
Bst4CI ACNGT 2 cut(s) 542, 670
BstC8I GCNNGC 3 cut(s) 96, 246, 396
BstDEI CTNAG 2 cut(s) 291, 839
BstDSI CCRYGG 2 cut(s) 9, 249
BstF5I GGATG 1 cut(s) 700
BstFNI CGCG 1 cut(s) 251
BstHHI GCGC 1 cut(s) 608
BstKTI GATC 1 cut(s) 909
BstMBI GATC 1 cut(s) 906
BstMWI GCNNNNNNNGC 1 cut(s) 91
BstNI CCWGG 1 cut(s) 424
BstNSI RCATGY 2 cut(s) 244, 436
BstSCI CCNGG 1 cut(s) 422
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 3 cut(s) 110, 113, 385
BstV2I GAAGAC 3 cut(s) 317, 543, 671
BstX2I RGATCY 1 cut(s) 906
BstYI RGATCY 1 cut(s) 906
BsuRI GGCC 2 cut(s) 248, 320
BtgI CCRYGG 2 cut(s) 9, 249
BtsCI GGATG 1 cut(s) 700
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 1 cut(s) 148
BveI ACCTGC 1 cut(s) 534
Cac8I GCNNGC 3 cut(s) 96, 246, 396
CfoI GCGC 1 cut(s) 608
Cfr13I GGNCC 2 cut(s) 318, 888
Cfr42I CCGCGG 1 cut(s) 252
CviAII CATG 3 cut(s) 10, 241, 433
DdeI CTNAG 2 cut(s) 291, 839
DpnI GATC 1 cut(s) 908
DpnII GATC 1 cut(s) 906
DraI TTTAAA 1 cut(s) 991
EaeI YGGCCR 1 cut(s) 246
Eco130I CCWWGG 2 cut(s) 9, 609
Eco47I GGWCC 1 cut(s) 888
Eco57I CTGAAG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 422
EcoT14I CCWWGG 2 cut(s) 9, 609
ErhI CCWWGG 2 cut(s) 9, 609
FaeI CATG 3 cut(s) 13, 244, 436
FatI CATG 3 cut(s) 9, 240, 432
FauNDI CATATG 1 cut(s) 884
Fnu4HI GCNGC 6 cut(s) 99, 102, 249, 399, 681, 821
FokI GGATG 1 cut(s) 707
Fsp4HI GCNGC 6 cut(s) 99, 102, 249, 399, 681, 821
GlaI GCGC 1 cut(s) 607
GluI GCNGC 6 cut(s) 99, 102, 249, 399, 681, 821
HaeIII GGCC 2 cut(s) 248, 320
HapII CCGG 1 cut(s) 162
HhaI GCGC 1 cut(s) 608
Hin1II CATG 3 cut(s) 13, 244, 436
Hin6I GCGC 1 cut(s) 606
HinP1I GCGC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 5 cut(s) 80, 269, 373, 404, 494
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 5 cut(s) 286, 442, 701, 796, 892
Hpy188III TCNNGA 2 cut(s) 626, 650
Hpy8I GTNNAC 1 cut(s) 625
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 3 cut(s) 203, 501, 509
HpyCH4III ACNGT 2 cut(s) 542, 670
HpyCH4V TGCA 3 cut(s) 141, 436, 933
HpyF10VI GCNNNNNNNGC 1 cut(s) 91
HpyF3I CTNAG 2 cut(s) 291, 839
Hsp92II CATG 3 cut(s) 13, 244, 436
HspAI GCGC 1 cut(s) 606
KspI CCGCGG 1 cut(s) 252
Kzo9I GATC 1 cut(s) 906
LmnI GCTCC 1 cut(s) 787
Lsp1109I GCAGC 3 cut(s) 110, 113, 385
LweI GCATC 2 cut(s) 630, 678
MaeIII GTNAC 1 cut(s) 551
MalI GATC 1 cut(s) 908
MboI GATC 1 cut(s) 906
MboII GAAGA 7 cut(s) 187, 190, 322, 524, 548, 676, 714
MflI RGATCY 1 cut(s) 906
MmeI TCCRAC 1 cut(s) 432
MseI TTAA 4 cut(s) 39, 590, 734, 990
MslI CAYNNNNRTG 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 424
MvaI CCWGG 1 cut(s) 424
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 91
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 884
NdeII GATC 1 cut(s) 906
NlaIII CATG 3 cut(s) 13, 244, 436
NspI RCATGY 2 cut(s) 244, 436
PfeI GAWTC 5 cut(s) 80, 269, 373, 404, 494
PflMI CCANNNNNTGG 1 cut(s) 885
PkrI GCNGC 6 cut(s) 100, 103, 250, 400, 682, 822
Psp6I CCWGG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 422
PspPI GGNCC 2 cut(s) 318, 888
PsuI RGATCY 1 cut(s) 906
RseI CAYNNNNRTG 1 cut(s) 630
SacII CCGCGG 1 cut(s) 252
SaqAI TTAA 4 cut(s) 39, 590, 734, 990
SatI GCNGC 6 cut(s) 99, 102, 249, 399, 681, 821
Sau3AI GATC 1 cut(s) 906
Sau96I GGNCC 2 cut(s) 318, 888
ScrFI CCNGG 1 cut(s) 424
SfaNI GCATC 2 cut(s) 630, 678
Sfr303I CCGCGG 1 cut(s) 252
SgrBI CCGCGG 1 cut(s) 252
SinI GGWCC 1 cut(s) 888
SmiI ATTTAAAT 1 cut(s) 991
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
SsiI CCGC 6 cut(s) 249, 251, 401, 584, 680, 820
StyD4I CCNGG 1 cut(s) 422
StyI CCWWGG 2 cut(s) 9, 609
SwaI ATTTAAAT 1 cut(s) 991
TaaI ACNGT 2 cut(s) 542, 670
TaqI TCGA 3 cut(s) 89, 118, 201
TauI GCSGC 3 cut(s) 251, 683, 823
TfiI GAWTC 5 cut(s) 80, 269, 373, 404, 494
Tru1I TTAA 4 cut(s) 39, 590, 734, 990
Tru9I TTAA 4 cut(s) 39, 590, 734, 990
TscAI CASTG 1 cut(s) 148
TseI GCWGC 3 cut(s) 98, 101, 398
TspDTI ATGAA 4 cut(s) 19, 744, 861, 907
TspGWI ACGGA 3 cut(s) 148, 190, 539
TspRI CASTG 1 cut(s) 148
Van91I CCANNNNNTGG 1 cut(s) 885
VpaK11BI GGWCC 1 cut(s) 888
XapI RAATTY 2 cut(s) 296, 575
XceI RCATGY 2 cut(s) 244, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.