Rh4DG001200

Chloroplast envelope membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004997
Physical Location & Seq
Forward (+)
276368 .. 282047
5680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG001200.1

Sequence Viewer

Length: 1080 bp
ATGTTGTCCATGGTCTTATGCGAGAACTTCATCTTTGTTAACAACCACAACAAGCAGACTTTGTTTTCGCACAAAATTGATTCGCAGTCGAGACTCGCAGCCGCAGCAGCAGCAGTTGAGGTGGGTCGAGGAGGAGGAGGACGGAGGTGCAGTGGGTTCGTTGCCAATGCCGGAAAGAGAAAACGGAAATGGTGGCAGAGCTTCTTCTTCGACGAGGAAGGTAATTGGCTTGGTTTGAAAGACGATGACATGCTGGCCGCGGAGGAAGAGGAGGAGAACTTGGATTCCAATTCCTACTCGGAAGCTGAGAAATTTGAGGCGTGGAAGACAAGGGCCGAAGCAATCGTGGATTTGAGGGAAGCACAGCAGGATATGAGCAACGAGGAGAATCGCAAGTGGGAGGATTGGCTTGCTGCGGATTCGAACTCGTCTCCCTCATCCTCGTCCTCGTCCTGGGAATACATGCAGTCTGATATTCCTACCGAAACTGAAGCCGAAAAGGGGGTGCTTTACTCTCTCACCGATTCCCTTCTTGGAAGGGAAGATGATGATGATGACCTGCTCTACGAAGACCGTGTGTTCCGTTACGCCTCCCTCAATTCGGCTAAATTTTTGGCGGTTTTAATAATTATACCCTGCGCCTTGGATTTTGTGGTTCACGACTATGTTTTGATGCCCTTTCTGGACAGATATGTGAAGACCGTGCCACTTGCAGCAGAGATGCTGGATGTCAGAAGAAATCAAAAGCTTCAAATGGTTGATGAATTAAAAATTGAGAGAGCAAGATTTCAGCTTGAGATGGAAATTGGTAAAGCTCCACCACTTTCTGATGAGGAGGTCTGGTGGGAGTTGCGGCATAAAGCATTAGAGCTGAGAGATGAATGGAGATTTGAAAATCGTAGAGCATTTGCCAACATATGGTCCGATATGTTATTTGGGATCTCATTATTCATTCTTTTATACTGCAACCAGAGTAAAGTAGCTTTGCTGAAATTTACAGGTTATAAGATAATAAATAATATTTCAGATACTGGGAAGGCATTCTTAATTATACTCATCACAGATATTTTTTTAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CemA

Protein Analysis

359

Amino Acids

41.39

Weight (kDa)

4.74

Isoelectric Point (pI)

51.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CemA PF03040 195 - 359 1.6e-42 CemA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1005
Acc36I ACCTGC 1 cut(s) 567
AccB7I CCANNNNNTGG 1 cut(s) 918
AccII CGCG 1 cut(s) 260
AciI CCGC 6 cut(s) 102, 258, 260, 416, 617, 853
AclWI GGATC 1 cut(s) 947
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 3 cut(s) 311, 608, 992
AcuI CTGAAG 1 cut(s) 510
AfiI CCNNNNNNNGG 4 cut(s) 453, 501, 601, 918
AgsI TTSAA 3 cut(s) 238, 752, 893
AjnI CCWGG 1 cut(s) 452
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 7 cut(s) 201, 305, 748, 793, 815, 871, 983
AluI AGCT 7 cut(s) 201, 305, 748, 793, 815, 871, 983
Alw26I GTCTC 2 cut(s) 85, 435
AlwI GGATC 1 cut(s) 947
AlwNI CAGNNNCTG 1 cut(s) 1031
AoxI GGCC 2 cut(s) 255, 333
ApeKI GCWGC 6 cut(s) 98, 104, 107, 110, 413, 713
ApoI RAATTY 3 cut(s) 311, 608, 992
ArsI GACNNNNNNTTYG 2 cut(s) 49, 81
Asp700I GAANNNNTTC 1 cut(s) 1040
AspLEI GCGC 1 cut(s) 641
AspS9I GGNCC 2 cut(s) 333, 921
AsuHPI GGTGA 1 cut(s) 511
AsuII TTCGAA 1 cut(s) 422
AvaII GGWCC 1 cut(s) 921
BbsI GAAGAC 3 cut(s) 332, 576, 704
BbvI GCAGC 6 cut(s) 110, 116, 119, 122, 400, 725
BccI CCATC 1 cut(s) 793
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BciT130I CCWGG 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 85, 435
BfuAI ACCTGC 1 cut(s) 567
BisI GCNGC 9 cut(s) 99, 102, 105, 108, 111, 258, 414, 714, 854
BlsI GCNGC 9 cut(s) 100, 103, 106, 109, 112, 259, 415, 715, 855
Bme1390I CCNGG 1 cut(s) 454
Bme18I GGWCC 1 cut(s) 921
BmgT120I GGNCC 2 cut(s) 333, 921
BmrFI CCNGG 1 cut(s) 454
BmrI ACTGGG 1 cut(s) 1041
BmsI GCATC 2 cut(s) 663, 711
BmuI ACTGGG 1 cut(s) 1041
BpiI GAAGAC 3 cut(s) 332, 576, 704
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 815
BsaJI CCNNGG 4 cut(s) 9, 258, 453, 642
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 827, 857
Bsc4I CCNNNNNNNGG 4 cut(s) 453, 501, 601, 918
Bse1I ACTGG 1 cut(s) 1036
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 4 cut(s) 9, 258, 453, 642
BseGI GGATG 2 cut(s) 437, 733
BseLI CCNNNNNNNGG 4 cut(s) 453, 501, 601, 918
BseMII CTCAG 2 cut(s) 297, 863
BseNI ACTGG 1 cut(s) 1036
BseRI GAGGAG 7 cut(s) 144, 147, 150, 284, 287, 398, 848
BseXI GCAGC 6 cut(s) 110, 116, 119, 122, 400, 725
BsgI GTGCAG 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 2 cut(s) 257, 335
BsiSI CCGG 1 cut(s) 171
BslI CCNNNNNNNGG 4 cut(s) 453, 501, 601, 918
BsmAI GTCTC 2 cut(s) 85, 435
BsmBI CGTCTC 1 cut(s) 435
BsmI GAATGC 1 cut(s) 1040
BsnI GGCC 2 cut(s) 257, 335
Bsp119I TTCGAA 1 cut(s) 422
Bsp143I GATC 1 cut(s) 939
Bsp19I CCATGG 1 cut(s) 9
BspACI CCGC 6 cut(s) 102, 258, 260, 416, 617, 853
BspANI GGCC 2 cut(s) 257, 335
BspCNI CTCAG 2 cut(s) 298, 864
BspFNI CGCG 1 cut(s) 260
BspMI ACCTGC 1 cut(s) 567
BspPI GGATC 1 cut(s) 947
BspT104I TTCGAA 1 cut(s) 422
BsrI ACTGG 1 cut(s) 1036
BssECI CCNNGG 4 cut(s) 9, 258, 453, 642
BssMI GATC 1 cut(s) 939
BssT1I CCWWGG 2 cut(s) 9, 642
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 2 cut(s) 575, 703
Bst6I CTCTTC 1 cut(s) 261
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 2 cut(s) 255, 411
BstDEI CTNAG 2 cut(s) 306, 872
BstDSI CCRYGG 2 cut(s) 9, 258
BstF5I GGATG 2 cut(s) 437, 733
BstFNI CGCG 1 cut(s) 260
BstHHI GCGC 1 cut(s) 641
BstKTI GATC 1 cut(s) 942
BstMAI GTCTC 2 cut(s) 85, 435
BstMBI GATC 1 cut(s) 939
BstMWI GCNNNNNNNGC 3 cut(s) 104, 107, 110
BstNI CCWGG 1 cut(s) 454
BstNSI RCATGY 2 cut(s) 253, 466
BstSCI CCNGG 1 cut(s) 452
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 6 cut(s) 110, 116, 119, 122, 400, 725
BstV2I GAAGAC 3 cut(s) 332, 576, 704
BstX2I RGATCY 1 cut(s) 939
BstYI RGATCY 1 cut(s) 939
BsuRI GGCC 2 cut(s) 257, 335
BtgI CCRYGG 2 cut(s) 9, 258
BtsCI GGATG 2 cut(s) 437, 733
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 157
BveI ACCTGC 1 cut(s) 567
Cac8I GCNNGC 2 cut(s) 255, 411
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 1 cut(s) 641
Cfr13I GGNCC 2 cut(s) 333, 921
Cfr42I CCGCGG 1 cut(s) 261
CviAII CATG 3 cut(s) 10, 250, 463
DdeI CTNAG 2 cut(s) 306, 872
DpnI GATC 1 cut(s) 941
DpnII GATC 1 cut(s) 939
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 9, 642
Eco47I GGWCC 1 cut(s) 921
Eco57I CTGAAG 1 cut(s) 510
EcoRII CCWGG 1 cut(s) 452
EcoT14I CCWWGG 2 cut(s) 9, 642
ErhI CCWWGG 2 cut(s) 9, 642
Esp3I CGTCTC 1 cut(s) 435
FaeI CATG 3 cut(s) 13, 253, 466
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FatI CATG 3 cut(s) 9, 249, 462
FauNDI CATATG 1 cut(s) 917
Fnu4HI GCNGC 9 cut(s) 99, 102, 105, 108, 111, 258, 414, 714, 854
FokI GGATG 2 cut(s) 424, 740
Fsp4HI GCNGC 9 cut(s) 99, 102, 105, 108, 111, 258, 414, 714, 854
GlaI GCGC 1 cut(s) 640
GluI GCNGC 9 cut(s) 99, 102, 105, 108, 111, 258, 414, 714, 854
HaeIII GGCC 2 cut(s) 257, 335
HapII CCGG 1 cut(s) 171
HhaI GCGC 1 cut(s) 641
Hin1II CATG 3 cut(s) 13, 253, 466
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 6 cut(s) 80, 93, 284, 388, 419, 524
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 1 cut(s) 171
HphI GGTGA 1 cut(s) 511
Hpy166II GTNNAC 2 cut(s) 40, 658
Hpy188I TCNGA 6 cut(s) 301, 472, 734, 829, 925, 1027
Hpy188III TCNNGA 3 cut(s) 90, 659, 683
Hpy8I GTNNAC 2 cut(s) 40, 658
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 4 cut(s) 212, 531, 539, 1030
HpyCH4III ACNGT 2 cut(s) 575, 703
HpyCH4V TGCA 4 cut(s) 150, 466, 713, 966
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 107, 110
HpyF3I CTNAG 2 cut(s) 306, 872
Hsp92II CATG 3 cut(s) 13, 253, 466
HspAI GCGC 1 cut(s) 639
KspAI GTTAAC 1 cut(s) 40
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 1 cut(s) 939
LmnI GCTCC 1 cut(s) 820
Lsp1109I GCAGC 6 cut(s) 110, 116, 119, 122, 400, 725
LweI GCATC 2 cut(s) 663, 711
MaeIII GTNAC 1 cut(s) 584
MalI GATC 1 cut(s) 941
MboI GATC 1 cut(s) 939
MboII GAAGA 8 cut(s) 196, 199, 278, 337, 554, 581, 709, 747
MflI RGATCY 1 cut(s) 939
MlyI GAGTC 1 cut(s) 87
MroXI GAANNNNTTC 1 cut(s) 1040
MseI TTAA 4 cut(s) 39, 623, 767, 1046
MslI CAYNNNNRTG 1 cut(s) 663
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 1 cut(s) 454
Mva1269I GAATGC 1 cut(s) 1040
MvaI CCWGG 1 cut(s) 454
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 3 cut(s) 104, 107, 110
NcoI CCATGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 917
NdeII GATC 1 cut(s) 939
NlaIII CATG 3 cut(s) 13, 253, 466
NspI RCATGY 2 cut(s) 253, 466
NspV TTCGAA 1 cut(s) 422
PctI GAATGC 1 cut(s) 1040
PdmI GAANNNNTTC 1 cut(s) 1040
PfeI GAWTC 5 cut(s) 80, 284, 388, 419, 524
PflMI CCANNNNNTGG 1 cut(s) 918
PkrI GCNGC 9 cut(s) 100, 103, 106, 109, 112, 259, 415, 715, 855
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PsiI TTATAA 1 cut(s) 1005
Psp6I CCWGG 1 cut(s) 452
PspGI CCWGG 1 cut(s) 452
PspPI GGNCC 2 cut(s) 333, 921
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 1 cut(s) 939
RseI CAYNNNNRTG 1 cut(s) 663
SacII CCGCGG 1 cut(s) 261
SaqAI TTAA 4 cut(s) 39, 623, 767, 1046
SatI GCNGC 9 cut(s) 99, 102, 105, 108, 111, 258, 414, 714, 854
Sau3AI GATC 1 cut(s) 939
Sau96I GGNCC 2 cut(s) 333, 921
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 454
SfaNI GCATC 2 cut(s) 663, 711
Sfr303I CCGCGG 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 422
SgrBI CCGCGG 1 cut(s) 261
SinI GGWCC 1 cut(s) 921
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
SsiI CCGC 6 cut(s) 102, 258, 260, 416, 617, 853
SspI AATATT 1 cut(s) 1021
StyD4I CCNGG 1 cut(s) 452
StyI CCWWGG 2 cut(s) 9, 642
TaaI ACNGT 2 cut(s) 575, 703
TaqI TCGA 4 cut(s) 89, 127, 210, 422
TauI GCSGC 3 cut(s) 104, 260, 856
TfiI GAWTC 5 cut(s) 80, 284, 388, 419, 524
Tru1I TTAA 4 cut(s) 39, 623, 767, 1046
Tru9I TTAA 4 cut(s) 39, 623, 767, 1046
TscAI CASTG 1 cut(s) 157
TseI GCWGC 6 cut(s) 98, 104, 107, 110, 413, 713
TspDTI ATGAA 4 cut(s) 19, 777, 894, 940
TspGWI ACGGA 3 cut(s) 157, 199, 572
TspRI CASTG 1 cut(s) 157
Van91I CCANNNNNTGG 1 cut(s) 918
VpaK11BI GGWCC 1 cut(s) 921
XapI RAATTY 3 cut(s) 311, 608, 992
XceI RCATGY 2 cut(s) 253, 466
XmnI GAANNNNTTC 1 cut(s) 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.