AT4G31290

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010804
Physical Location & Seq
Forward (+)
15185775 .. 15188484
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31290.1

Sequence Viewer

Length: 684 bp
ATGGTGATGTGGGTCTTTGGCTATGGCTCTCTTGTGTGGAACCCAGGATTTCACTACGATGAGAAAGTGTTAGGTTTCATCAAGGGATATAAACGTGTCTTTGATCTTGCTTGCATTGATCATAGAGGTACACCAGAACACCCTGCAAGAACTTGCACCCTCGAGAAAGCTGAAGAAGCCATATGCTGGGGTACTGCATTCTGTGTCCGTGGAGGACCAGAAAAAGAACGTCTGGCTATGGAGTACTTGGAACGTAGAGAGTGTGAATATGATCTCAAGACAAGTGTAGACTTTTACAAGGAAGATGATCCTCTAAAGCCAGCTGTAACTGGAGTGATAGTATTCACTTCTACTCCTGACAAGGTCTCCAACAAGTATTATCTCGGACCTGCGCCATTAGAAGACATGGCAAGACAAATCGCGACAGCCAATGGACCATGTGGTAACAACAGAGATTATCTCTTCCTGCTCGAGAAGGCAATGCACGACATTGGGCATGAGGAGGACTATGTTATAGAGCTGGCAAACGAGGTGAGGAAGGTTCTGGCCGAGTCCTCGACTAAGAAGGTGACACCGGTGAAGGAATCAAGAGCAAGCCGTGTAGCTAACAAGTCGAAGAACAATGTCCCCACGGCTCATCAGATACTACCTCATCATCCAGAAGCTGTTGCCACTACAATATAA

Protein Analysis

227

Amino Acids

25.61

Weight (kDa)

6.08

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 180 6.6e-47 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 397
AccB7I CCANNNNNTGG 1 cut(s) 186
AccI GTMKAC 1 cut(s) 288
AccII CGCG 1 cut(s) 422
AclWI GGATC 1 cut(s) 302
AcoI YGGCCR 1 cut(s) 546
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 3 cut(s) 130, 193, 245
AfiI CCNNNNNNNGG 1 cut(s) 186
AflIII ACRYGT 1 cut(s) 94
AgeI ACCGGT 1 cut(s) 574
AjnI CCWGG 1 cut(s) 43
AluBI AGCT 5 cut(s) 170, 323, 520, 605, 665
AluI AGCT 5 cut(s) 170, 323, 520, 605, 665
Alw26I GTCTC 1 cut(s) 370
AlwI GGATC 1 cut(s) 302
AlwNI CAGNNNCTG 1 cut(s) 665
Ama87I CYCGRG 2 cut(s) 161, 470
AoxI GGCC 1 cut(s) 546
AsiGI ACCGGT 1 cut(s) 574
AspLEI GCGC 1 cut(s) 394
AspS9I GGNCC 3 cut(s) 215, 386, 434
AsuHPI GGTGA 4 cut(s) 16, 544, 580, 589
AvaI CYCGRG 2 cut(s) 161, 470
AvaII GGWCC 3 cut(s) 215, 386, 434
BbsI GAAGAC 1 cut(s) 408
BceAI ACGGC 2 cut(s) 582, 648
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 370
BfuAI ACCTGC 1 cut(s) 397
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 3 cut(s) 215, 386, 434
BmeT110I CYCGRG 2 cut(s) 161, 470
BmgT120I GGNCC 3 cut(s) 215, 386, 434
BmiI GGNNCC 1 cut(s) 41
BmrFI CCNGG 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 408
BplI GAGNNNNNCTC 2 cut(s) 444, 476
BpmI CTGGAG 1 cut(s) 351
BpuEI CTTGAG 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 370
BsaJI CCNNGG 3 cut(s) 43, 208, 630
BsaWI WCCGGW 1 cut(s) 574
BsaXI ACNNNNNCTCC 6 cut(s) 324, 337, 350, 354, 367, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse118I RCCGGY 1 cut(s) 574
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 43, 208, 630
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 486
BseNI ACTGG 1 cut(s) 334
BseRI GAGGAG 1 cut(s) 515
BseYI CCCAGC 1 cut(s) 186
Bsh1236I CGCG 1 cut(s) 422
BshFI GGCC 1 cut(s) 548
BshTI ACCGGT 1 cut(s) 574
BsiHKCI CYCGRG 2 cut(s) 161, 470
BsiSI CCGG 1 cut(s) 575
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 611
BsmI GAATGC 1 cut(s) 197
BsnI GGCC 1 cut(s) 548
Bso31I GGTCTC 1 cut(s) 370
BsoBI CYCGRG 2 cut(s) 161, 470
Bsp143I GATC 4 cut(s) 103, 118, 271, 307
Bsp68I TCGCGA 1 cut(s) 422
BspANI GGCC 1 cut(s) 548
BspFNI CGCG 1 cut(s) 422
BspLI GGNNCC 1 cut(s) 41
BspMI ACCTGC 1 cut(s) 397
BspPI GGATC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 1 cut(s) 574
BsrI ACTGG 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 574
BssECI CCNNGG 3 cut(s) 43, 208, 630
BssMI GATC 4 cut(s) 103, 118, 271, 307
Bst2UI CCWGG 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 467
BstC8I GCNNGC 4 cut(s) 112, 321, 522, 595
BstDEI CTNAG 1 cut(s) 561
BstDSI CCRYGG 2 cut(s) 208, 630
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 1 cut(s) 422
BstHHI GCGC 1 cut(s) 394
BstKTI GATC 4 cut(s) 106, 121, 274, 310
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 4 cut(s) 103, 118, 271, 307
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 43
BstUI CGCG 1 cut(s) 422
BstV2I GAAGAC 1 cut(s) 408
BsuRI GGCC 1 cut(s) 548
BtgI CCRYGG 2 cut(s) 208, 630
BtsCI GGATG 1 cut(s) 655
BtuMI TCGCGA 1 cut(s) 422
BveI ACCTGC 1 cut(s) 397
Cac8I GCNNGC 4 cut(s) 112, 321, 522, 595
CaiI CAGNNNCTG 1 cut(s) 665
CfoI GCGC 1 cut(s) 394
Cfr10I RCCGGY 1 cut(s) 574
Cfr13I GGNCC 3 cut(s) 215, 386, 434
Csp6I GTAC 3 cut(s) 129, 192, 244
CspAI ACCGGT 1 cut(s) 574
CviAII CATG 3 cut(s) 406, 438, 497
CviQI GTAC 3 cut(s) 129, 192, 244
DdeI CTNAG 1 cut(s) 561
DpnI GATC 4 cut(s) 105, 120, 273, 309
DpnII GATC 4 cut(s) 103, 118, 271, 307
EaeI YGGCCR 1 cut(s) 546
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Eco31I GGTCTC 1 cut(s) 370
Eco47I GGWCC 3 cut(s) 215, 386, 434
Eco57I CTGAAG 1 cut(s) 192
Eco88I CYCGRG 2 cut(s) 161, 470
EcoRII CCWGG 1 cut(s) 43
FaeI CATG 3 cut(s) 409, 441, 500
FaqI GGGAC 1 cut(s) 611
FatI CATG 3 cut(s) 405, 437, 496
FauNDI CATATG 1 cut(s) 182
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 288
FokI GGATG 1 cut(s) 642
GlaI GCGC 1 cut(s) 393
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 351
HaeIII GGCC 1 cut(s) 548
HapII CCGG 1 cut(s) 575
HhaI GCGC 1 cut(s) 394
Hin1II CATG 3 cut(s) 409, 441, 500
Hin6I GCGC 1 cut(s) 392
HinP1I GCGC 1 cut(s) 392
HinfI GANTC 2 cut(s) 551, 584
HpaII CCGG 1 cut(s) 575
HphI GGTGA 4 cut(s) 16, 544, 580, 589
Hpy166II GTNNAC 2 cut(s) 131, 289
Hpy188I TCNGA 2 cut(s) 386, 642
Hpy188III TCNNGA 7 cut(s) 163, 277, 356, 421, 472, 588, 659
Hpy8I GTNNAC 2 cut(s) 131, 289
HpyAV CCTTC 4 cut(s) 469, 532, 559, 574
HpyCH4IV ACGT 3 cut(s) 94, 229, 253
HpyCH4V TGCA 5 cut(s) 114, 146, 156, 197, 484
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 3 cut(s) 94, 229, 253
Hsp92II CATG 3 cut(s) 409, 441, 500
HspAI GCGC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 4 cut(s) 103, 118, 271, 307
MaeII ACGT 3 cut(s) 94, 229, 253
MaeIII GTNAC 3 cut(s) 325, 443, 568
MalI GATC 4 cut(s) 105, 120, 273, 309
MboI GATC 4 cut(s) 103, 118, 271, 307
MboII GAAGA 5 cut(s) 185, 314, 413, 454, 628
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 1 cut(s) 393
MslI CAYNNNNRTG 1 cut(s) 57
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 1 cut(s) 575
MspR9I CCNGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 197
MvaI CCWGG 1 cut(s) 45
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 1 cut(s) 176
NdeI CATATG 1 cut(s) 182
NdeII GATC 4 cut(s) 103, 118, 271, 307
NlaIII CATG 3 cut(s) 409, 441, 500
NlaIV GGNNCC 1 cut(s) 41
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 1 cut(s) 568
NruI TCGCGA 1 cut(s) 422
PaeR7I CTCGAG 2 cut(s) 161, 470
PctI GAATGC 1 cut(s) 197
PfeI GAWTC 1 cut(s) 584
PflFI GACNNNGTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 186
PinAI ACCGGT 1 cut(s) 574
PleI GAGTC 1 cut(s) 559
PpsI GAGTC 1 cut(s) 559
Psp6I CCWGG 1 cut(s) 43
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 41
PspPI GGNCC 3 cut(s) 215, 386, 434
PstNI CAGNNNCTG 1 cut(s) 665
PsyI GACNNNGTC 1 cut(s) 362
PvuII CAGCTG 1 cut(s) 323
RruI TCGCGA 1 cut(s) 422
RsaI GTAC 3 cut(s) 130, 193, 245
RsaNI GTAC 3 cut(s) 129, 192, 244
RseI CAYNNNNRTG 1 cut(s) 57
Sau3AI GATC 4 cut(s) 103, 118, 271, 307
Sau96I GGNCC 3 cut(s) 215, 386, 434
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 1 cut(s) 45
Sfr274I CTCGAG 2 cut(s) 161, 470
SgrAI CRCCGGYG 1 cut(s) 574
SinI GGWCC 3 cut(s) 215, 386, 434
SlaI CTCGAG 2 cut(s) 161, 470
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 3 cut(s) 161, 275, 470
SmoI CTYRAG 3 cut(s) 161, 275, 470
StyD4I CCNGG 1 cut(s) 43
TaiI ACGT 3 cut(s) 97, 232, 256
TaqI TCGA 4 cut(s) 162, 471, 557, 614
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 1 cut(s) 584
TseFI GTSAC 1 cut(s) 568
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 1 cut(s) 67
TspGWI ACGGA 1 cut(s) 197
Tth111I GACNNNGTC 1 cut(s) 362
Van91I CCANNNNNTGG 1 cut(s) 186
VpaK11BI GGWCC 3 cut(s) 215, 386, 434
XhoI CTCGAG 2 cut(s) 161, 470
XmiI GTMKAC 1 cut(s) 288
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.