RchiOBHm_Chr6g0305381

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010804
Physical Location & Seq
Forward (+)
64397958 .. 64400418
2461 bp
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UTR
Exon/CDS
Intron
PRQ27444

Sequence Viewer

Length: 684 bp
ATGGTATTCTGGGTGTTTGGATATGGTTCACTTGTGTGGAACCCTGGTTTTGAATACGACGAGAAAGTGATAGGTTTCATCAAGGACTACAGGCGTGTATTCGATCTTGCTTGCATTGATCATCGAGGTACGCCTGAGCACCCTGCAAGAACTTGCACATTGGAACACCAAGAAGGGGCTATTTGCTGGGGTGCTGCTTATTCTGTGTGTGGCGGCCCTGAAAGGGAAAGACTGGCTATGGAGTACTTGGAAAGGAGAGAATGTGAATATGATGCAAAGACTCTTGTGAACTTTTACAAAGAAGGGGACTTCTCTGATGAGCCTTCTTTGACAGGAGTCATTGTTTTCACATCTACTCCAGACAAAGAATCAAACAAATATTACCTGGGTCCTGCTCCATTGGAGGATATGGCAAGCCAAATTGCGACTGCTTTTGGGCCATGCGGGAACAACAGAGACTACTTATTTTTGCTGGAAAAAGCTATGTTTGATATTGGCCATGAAGATGACTATGTGATAGAGCTTGCAAATGAGGTGAGGAATGTACTTGGAATTGTTGGGAAAGGAATTCCAGGAATTCCAAGGGATAAGAAGTTGACAGGACCAACCCACATCCCGCTCAAGTCCCACATTCCAACCATTCAATTGCATCCCCTTCCGGAAGCCATCGCCATGGACTCGTAG

Protein Analysis

227

Amino Acids

25.52

Weight (kDa)

4.94

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 181 1.7e-44 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 619
AccIII TCCGGA 1 cut(s) 658
AciI CCGC 3 cut(s) 213, 444, 617
AcoI YGGCCR 1 cut(s) 496
AcsI RAATTY 2 cut(s) 567, 576
AfaI GTAC 3 cut(s) 130, 245, 546
AfiI CCNNNNNNNGG 2 cut(s) 175, 223
AgsI TTSAA 2 cut(s) 53, 644
AjnI CCWGG 3 cut(s) 43, 384, 571
AleI CACNNNNGTG 1 cut(s) 34
AluBI AGCT 2 cut(s) 482, 523
AluI AGCT 2 cut(s) 482, 523
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 450
Aor13HI TCCGGA 1 cut(s) 658
AoxI GGCC 3 cut(s) 214, 437, 496
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 2 cut(s) 567, 576
AspS9I GGNCC 4 cut(s) 215, 389, 437, 602
AsuHPI GGTGA 1 cut(s) 547
AvaII GGWCC 2 cut(s) 389, 602
BalI TGGCCA 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 674
BciT130I CCWGG 3 cut(s) 45, 386, 573
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 450
BfmI CTRYAG 1 cut(s) 88
BisI GCNGC 2 cut(s) 195, 214
BlsI GCNGC 2 cut(s) 196, 215
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 3 cut(s) 45, 386, 573
Bme18I GGWCC 2 cut(s) 389, 602
BmgT120I GGNCC 4 cut(s) 215, 389, 437, 602
BmiI GGNNCC 2 cut(s) 41, 390
BmrFI CCNGG 3 cut(s) 45, 386, 573
BmsI GCATC 2 cut(s) 262, 658
BoxI GACNNNNGTC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 605
BsaJI CCNNGG 4 cut(s) 43, 385, 581, 672
BsaWI WCCGGW 1 cut(s) 658
BsaXI ACNNNNNCTCC 6 cut(s) 247, 277, 327, 340, 357, 370
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 223
Bse1I ACTGG 1 cut(s) 237
BseAI TCCGGA 1 cut(s) 658
BseBI CCWGG 3 cut(s) 45, 386, 573
BseDI CCNNGG 4 cut(s) 43, 385, 581, 672
BseGI GGATG 2 cut(s) 612, 649
BseLI CCNNNNNNNGG 2 cut(s) 175, 223
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 186
BshFI GGCC 3 cut(s) 216, 439, 498
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 659
BslFI GGGAC 2 cut(s) 320, 610
BslI CCNNNNNNNGG 2 cut(s) 175, 223
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 2 cut(s) 320, 610
BsnI GGCC 3 cut(s) 216, 439, 498
Bsp1286I GDGCHC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 658
Bsp143I GATC 2 cut(s) 103, 118
Bsp19I CCATGG 1 cut(s) 672
BspACI CCGC 3 cut(s) 213, 444, 617
BspANI GGCC 3 cut(s) 216, 439, 498
BspCNI CTCAG 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 658
BspLI GGNNCC 2 cut(s) 41, 390
BsrBI CCGCTC 1 cut(s) 619
BsrI ACTGG 1 cut(s) 237
BssECI CCNNGG 4 cut(s) 43, 385, 581, 672
BssMI GATC 2 cut(s) 103, 118
BssT1I CCWWGG 2 cut(s) 581, 672
Bst2UI CCWGG 3 cut(s) 45, 386, 573
BstC8I GCNNGC 3 cut(s) 112, 415, 525
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 672
BstF5I GGATG 2 cut(s) 612, 649
BstKTI GATC 2 cut(s) 106, 121
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 2 cut(s) 103, 118
BstNI CCWGG 3 cut(s) 45, 386, 573
BstPAI GACNNNNGTC 1 cut(s) 335
BstSCI CCNGG 3 cut(s) 43, 384, 571
BstSFI CTRYAG 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 673
BsuRI GGCC 3 cut(s) 216, 439, 498
BtgI CCRYGG 1 cut(s) 672
BtgZI GCGATG 1 cut(s) 652
BtsCI GGATG 2 cut(s) 612, 649
Cac8I GCNNGC 3 cut(s) 112, 415, 525
Cfr13I GGNCC 4 cut(s) 215, 389, 437, 602
Csp6I GTAC 3 cut(s) 129, 244, 545
CviAII CATG 3 cut(s) 441, 500, 673
CviQI GTAC 3 cut(s) 129, 244, 545
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 105, 120
DpnII GATC 2 cut(s) 103, 118
EaeI YGGCCR 1 cut(s) 496
Eco130I CCWWGG 2 cut(s) 581, 672
Eco47I GGWCC 2 cut(s) 389, 602
EcoO109I RGGNCCY 1 cut(s) 389
EcoRI GAATTC 2 cut(s) 567, 576
EcoRII CCWGG 3 cut(s) 43, 384, 571
EcoT14I CCWWGG 2 cut(s) 581, 672
ErhI CCWWGG 2 cut(s) 581, 672
FaeI CATG 3 cut(s) 444, 503, 676
FaiI YATR 9 cut(s) 24, 239, 270, 410, 442, 485, 501, 513, 674
FaqI GGGAC 2 cut(s) 320, 610
FatI CATG 3 cut(s) 440, 499, 672
FauI CCCGC 2 cut(s) 437, 624
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 2 cut(s) 195, 214
FokI GGATG 2 cut(s) 599, 636
Fsp4HI GCNGC 2 cut(s) 195, 214
GluI GCNGC 2 cut(s) 195, 214
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 3 cut(s) 216, 439, 498
HapII CCGG 1 cut(s) 659
Hin1II CATG 3 cut(s) 444, 503, 676
HincII GTYRAC 1 cut(s) 597
HindII GTYRAC 1 cut(s) 597
HinfI GANTC 4 cut(s) 280, 336, 368, 677
HpaII CCGG 1 cut(s) 659
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 3 cut(s) 29, 289, 597
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 359, 659
Hpy8I GTNNAC 3 cut(s) 29, 289, 597
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 4 cut(s) 167, 296, 333, 665
HpyCH4V TGCA 6 cut(s) 114, 146, 156, 275, 527, 649
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 3 cut(s) 444, 503, 676
Kpn2I TCCGGA 1 cut(s) 658
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 103, 118
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 2 cut(s) 262, 658
MalI GATC 2 cut(s) 105, 120
MbiI CCGCTC 1 cut(s) 619
MboI GATC 2 cut(s) 103, 118
MboII GAAGA 1 cut(s) 515
MfeI CAATTG 1 cut(s) 644
MhlI GDGCHC 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 5 cut(s) 420, 552, 567, 576, 644
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 3 cut(s) 274, 345, 671
MmeI TCCRAC 1 cut(s) 659
MnlI CCTC 4 cut(s) 119, 397, 526, 531
Mox20I TGGCCA 1 cut(s) 498
MroI TCCGGA 1 cut(s) 658
MscI TGGCCA 1 cut(s) 498
MslI CAYNNNNRTG 3 cut(s) 34, 504, 671
Msp20I TGGCCA 1 cut(s) 498
MspI CCGG 1 cut(s) 659
MspR9I CCNGG 3 cut(s) 45, 386, 573
MunI CAATTG 1 cut(s) 644
MvaI CCWGG 3 cut(s) 45, 386, 573
NcoI CCATGG 1 cut(s) 672
NdeII GATC 2 cut(s) 103, 118
NlaIII CATG 3 cut(s) 444, 503, 676
NlaIV GGNNCC 2 cut(s) 41, 390
OliI CACNNNNGTG 1 cut(s) 34
PfeI GAWTC 1 cut(s) 368
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 2 cut(s) 196, 215
PleI GAGTC 3 cut(s) 274, 344, 671
PpsI GAGTC 3 cut(s) 274, 344, 671
PpuMI RGGWCCY 1 cut(s) 389
PshAI GACNNNNGTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 3 cut(s) 43, 384, 571
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 3 cut(s) 43, 384, 571
PspN4I GGNNCC 2 cut(s) 41, 390
PspPI GGNCC 4 cut(s) 215, 389, 437, 602
PspPPI RGGWCCY 1 cut(s) 389
RsaI GTAC 3 cut(s) 130, 245, 546
RsaNI GTAC 3 cut(s) 129, 244, 545
RseI CAYNNNNRTG 3 cut(s) 34, 504, 671
SatI GCNGC 2 cut(s) 195, 214
Sau3AI GATC 2 cut(s) 103, 118
Sau96I GGNCC 4 cut(s) 215, 389, 437, 602
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 3 cut(s) 274, 345, 671
ScrFI CCNGG 3 cut(s) 45, 386, 573
SduI GDGCHC 1 cut(s) 141
SetI ASST 6 cut(s) 76, 130, 387, 484, 525, 537
SfaNI GCATC 2 cut(s) 262, 658
SfcI CTRYAG 1 cut(s) 88
SinI GGWCC 2 cut(s) 389, 602
SmiMI CAYNNNNRTG 3 cut(s) 34, 504, 671
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 5 cut(s) 420, 552, 567, 576, 644
SsiI CCGC 3 cut(s) 213, 444, 617
SspI AATATT 1 cut(s) 380
StyD4I CCNGG 3 cut(s) 43, 384, 571
StyI CCWWGG 2 cut(s) 581, 672
TaqI TCGA 2 cut(s) 102, 124
TasI AATT 5 cut(s) 420, 552, 567, 576, 644
TatI WGTACW 2 cut(s) 243, 544
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 368
TseI GCWGC 1 cut(s) 194
TspDTI ATGAA 2 cut(s) 67, 516
VpaK11BI GGWCC 2 cut(s) 389, 602
XapI RAATTY 2 cut(s) 567, 576
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.