RLG00000010895

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010804
Physical Location & Seq
Reverse (-)
5129960 .. 5132751
2792 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010895

Sequence Viewer

Length: 756 bp
ATGATAAGATACGATAGCCTTCTCGGTCGGATCACACCACACGCTTTCCTCGCATTTCCTAATCGAGTTATAAAACCAACCATGGTATTCTGGGTGTTTGGATATGGTTCACTTGTGTGGAACCCTGGTTTTGAATACGACGAGAAAGTGATAGGTTTCATCAAGGACTACAGGCGTGTATTCGATCTTGCTTGCATTGATCATCGAGGTACGCCTGACCACCCTGCAAGAACTTGCACATTGGAACACCAAGAAGGGGCTATTTGCTGGGGTGCTGCTTATTCTGTGCGTGGTGGCCCTGAAAGGGAAAGACTGGCTATGGAGTACTTGGAAAGGAGAGAATGTGAATATGATGCAAAGACTCTTGTGAACTTTTACAAGGAAGGGGACTTCTCTGATGAGCCTTCTTTGACAGGAGTCATTGTTTTCACATCTACTCCAGACAAAGAATCAAACAAATATTACCTGGGTCCTGCTCCATTGGAGGATATGGCAAGCCAAATTGCGACTGCTTTTGGGCCATGCGGGAACAACAGAGACTACTTATTTTTGCTGGAAAAAGCTATGTTTGATATTGGCCATGAAGATGACTATGTGATAGAGCTTGCAAATGAGGTGAGGAATGTACTTGGAATTGTTGGGAAAGGAATTCCAAGGGATAAGAAGTTGACAGGACCAACCCACATCCCGCTTAAGTCCCACATTCCAACCATTCAGTTGCATCCCCTTCCGGAAGCCATCGCCATGGACTCATAG

Protein Analysis

252

Amino Acids

28.41

Weight (kDa)

5.4

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 31 - 208 2e-44 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 71
AccIII TCCGGA 1 cut(s) 730
AciI CCGC 2 cut(s) 525, 689
AclWI GGATC 1 cut(s) 38
AcoI YGGCCR 1 cut(s) 577
AcsI RAATTY 1 cut(s) 648
AfaI GTAC 3 cut(s) 211, 326, 627
AfiI CCNNNNNNNGG 2 cut(s) 256, 304
AflII CTTAAG 1 cut(s) 692
AgsI TTSAA 1 cut(s) 134
AjnI CCWGG 2 cut(s) 124, 465
AleI CACNNNNGTG 1 cut(s) 115
AluBI AGCT 2 cut(s) 563, 604
AluI AGCT 2 cut(s) 563, 604
Alw26I GTCTC 1 cut(s) 531
AlwI GGATC 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 730
AoxI GGCC 3 cut(s) 295, 518, 577
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 648
AspS9I GGNCC 4 cut(s) 296, 470, 518, 674
AsuHPI GGTGA 1 cut(s) 628
AvaII GGWCC 2 cut(s) 470, 674
BalI TGGCCA 1 cut(s) 579
BbvI GCAGC 1 cut(s) 262
BccI CCATC 1 cut(s) 746
BciT130I CCWGG 2 cut(s) 126, 467
BclI TGATCA 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 531
BfmI CTRYAG 1 cut(s) 169
BfrI CTTAAG 1 cut(s) 692
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 2 cut(s) 126, 467
Bme18I GGWCC 2 cut(s) 470, 674
BmgT120I GGNCC 4 cut(s) 296, 470, 518, 674
BmiI GGNNCC 2 cut(s) 122, 471
BmrFI CCNGG 2 cut(s) 126, 467
BmsI GCATC 2 cut(s) 343, 730
BoxI GACNNNNGTC 1 cut(s) 416
BpmI CTGGAG 1 cut(s) 423
BsaJI CCNNGG 5 cut(s) 81, 124, 466, 653, 744
BsaWI WCCGGW 1 cut(s) 730
BsaXI ACNNNNNCTCC 6 cut(s) 328, 358, 408, 421, 438, 451
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 304
Bse1I ACTGG 1 cut(s) 318
BseAI TCCGGA 1 cut(s) 730
BseBI CCWGG 2 cut(s) 126, 467
BseDI CCNNGG 5 cut(s) 81, 124, 466, 653, 744
BseGI GGATG 2 cut(s) 684, 721
BseLI CCNNNNNNNGG 2 cut(s) 256, 304
BseNI ACTGG 1 cut(s) 318
BseXI GCAGC 1 cut(s) 262
BseYI CCCAGC 1 cut(s) 267
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 3 cut(s) 297, 520, 579
BsiEI CGRYCG 1 cut(s) 28
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 2 cut(s) 401, 682
BslI CCNNNNNNNGG 2 cut(s) 256, 304
BsmAI GTCTC 1 cut(s) 531
BsmFI GGGAC 2 cut(s) 401, 682
BsnI GGCC 3 cut(s) 297, 520, 579
Bsp13I TCCGGA 1 cut(s) 730
Bsp143I GATC 3 cut(s) 30, 184, 199
Bsp19I CCATGG 2 cut(s) 81, 744
BspACI CCGC 2 cut(s) 525, 689
BspANI GGCC 3 cut(s) 297, 520, 579
BspEI TCCGGA 1 cut(s) 730
BspLI GGNNCC 2 cut(s) 122, 471
BspPI GGATC 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 692
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 5 cut(s) 81, 124, 466, 653, 744
BssMI GATC 3 cut(s) 30, 184, 199
BssT1I CCWWGG 3 cut(s) 81, 653, 744
Bst2UI CCWGG 2 cut(s) 126, 467
BstAFI CTTAAG 1 cut(s) 692
BstC8I GCNNGC 3 cut(s) 193, 496, 606
BstDSI CCRYGG 2 cut(s) 81, 744
BstF5I GGATG 2 cut(s) 684, 721
BstKTI GATC 3 cut(s) 33, 187, 202
BstMAI GTCTC 1 cut(s) 531
BstMBI GATC 3 cut(s) 30, 184, 199
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 2 cut(s) 126, 467
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 124, 465
BstSFI CTRYAG 1 cut(s) 169
BstV1I GCAGC 1 cut(s) 262
BstXI CCANNNNNNTGG 1 cut(s) 745
BsuRI GGCC 3 cut(s) 297, 520, 579
BtgI CCRYGG 2 cut(s) 81, 744
BtgZI GCGATG 1 cut(s) 724
BtsCI GGATG 2 cut(s) 684, 721
Cac8I GCNNGC 3 cut(s) 193, 496, 606
Cfr13I GGNCC 4 cut(s) 296, 470, 518, 674
Csp6I GTAC 3 cut(s) 210, 325, 626
CviAII CATG 4 cut(s) 82, 522, 581, 745
CviQI GTAC 3 cut(s) 210, 325, 626
DpnI GATC 3 cut(s) 32, 186, 201
DpnII GATC 3 cut(s) 30, 184, 199
EaeI YGGCCR 1 cut(s) 577
Eco130I CCWWGG 3 cut(s) 81, 653, 744
Eco47I GGWCC 2 cut(s) 470, 674
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 648
EcoRII CCWGG 2 cut(s) 124, 465
EcoT14I CCWWGG 3 cut(s) 81, 653, 744
ErhI CCWWGG 3 cut(s) 81, 653, 744
FaeI CATG 4 cut(s) 85, 525, 584, 748
FaqI GGGAC 2 cut(s) 401, 682
FatI CATG 4 cut(s) 81, 521, 580, 744
FauI CCCGC 2 cut(s) 518, 696
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 2 cut(s) 671, 708
Fsp4HI GCNGC 1 cut(s) 276
GluI GCNGC 1 cut(s) 276
GsaI CCCAGC 1 cut(s) 271
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 3 cut(s) 297, 520, 579
HapII CCGG 1 cut(s) 731
Hin1II CATG 4 cut(s) 85, 525, 584, 748
HincII GTYRAC 1 cut(s) 669
HindII GTYRAC 1 cut(s) 669
HinfI GANTC 4 cut(s) 361, 417, 449, 749
HpaII CCGG 1 cut(s) 731
HphI GGTGA 1 cut(s) 628
Hpy166II GTNNAC 3 cut(s) 110, 370, 669
Hpy188I TCNGA 2 cut(s) 30, 397
Hpy188III TCNNGA 2 cut(s) 440, 731
Hpy8I GTNNAC 3 cut(s) 110, 370, 669
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 5 cut(s) 29, 248, 377, 414, 737
HpyCH4V TGCA 6 cut(s) 195, 227, 237, 356, 608, 721
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
Hsp92II CATG 4 cut(s) 85, 525, 584, 748
Kpn2I TCCGGA 1 cut(s) 730
Ksp22I TGATCA 1 cut(s) 199
Kzo9I GATC 3 cut(s) 30, 184, 199
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 2 cut(s) 343, 730
MalI GATC 3 cut(s) 32, 186, 201
MboI GATC 3 cut(s) 30, 184, 199
MboII GAAGA 1 cut(s) 596
MlsI TGGCCA 1 cut(s) 579
MluCI AATT 3 cut(s) 501, 633, 648
MluNI TGGCCA 1 cut(s) 579
MlyI GAGTC 3 cut(s) 355, 426, 743
MmeI TCCRAC 2 cut(s) 8, 731
MnlI CCTC 5 cut(s) 59, 200, 478, 607, 612
Mox20I TGGCCA 1 cut(s) 579
MroI TCCGGA 1 cut(s) 730
MscI TGGCCA 1 cut(s) 579
MseI TTAA 1 cut(s) 693
MslI CAYNNNNRTG 3 cut(s) 115, 585, 743
Msp20I TGGCCA 1 cut(s) 579
MspCI CTTAAG 1 cut(s) 692
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 2 cut(s) 126, 467
MvaI CCWGG 2 cut(s) 126, 467
MwoI GCNNNNNNNGC 1 cut(s) 50
NcoI CCATGG 2 cut(s) 81, 744
NdeII GATC 3 cut(s) 30, 184, 199
NlaIII CATG 4 cut(s) 85, 525, 584, 748
NlaIV GGNNCC 2 cut(s) 122, 471
OliI CACNNNNGTG 1 cut(s) 115
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 3 cut(s) 355, 425, 743
PpsI GAGTC 3 cut(s) 355, 425, 743
PpuMI RGGWCCY 1 cut(s) 470
PshAI GACNNNNGTC 1 cut(s) 416
PsiI TTATAA 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 2 cut(s) 124, 465
PspFI CCCAGC 1 cut(s) 267
PspGI CCWGG 2 cut(s) 124, 465
PspN4I GGNNCC 2 cut(s) 122, 471
PspPI GGNCC 4 cut(s) 296, 470, 518, 674
PspPPI RGGWCCY 1 cut(s) 470
RsaI GTAC 3 cut(s) 211, 326, 627
RsaNI GTAC 3 cut(s) 210, 325, 626
RseI CAYNNNNRTG 3 cut(s) 115, 585, 743
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 3 cut(s) 30, 184, 199
Sau96I GGNCC 4 cut(s) 296, 470, 518, 674
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 3 cut(s) 355, 426, 743
ScrFI CCNGG 2 cut(s) 126, 467
SetI ASST 6 cut(s) 157, 211, 468, 565, 606, 618
SfaNI GCATC 2 cut(s) 343, 730
SfcI CTRYAG 1 cut(s) 169
SinI GGWCC 2 cut(s) 470, 674
SmiMI CAYNNNNRTG 3 cut(s) 115, 585, 743
SmlI CTYRAG 1 cut(s) 692
SmoI CTYRAG 1 cut(s) 692
Sse9I AATT 3 cut(s) 501, 633, 648
SsiI CCGC 2 cut(s) 525, 689
SspI AATATT 1 cut(s) 461
StyD4I CCNGG 2 cut(s) 124, 465
StyI CCWWGG 3 cut(s) 81, 653, 744
TaqI TCGA 3 cut(s) 64, 183, 205
TaqII GACCGA 1 cut(s) 14
TasI AATT 3 cut(s) 501, 633, 648
TatI WGTACW 2 cut(s) 324, 625
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 2 cut(s) 148, 597
Vha464I CTTAAG 1 cut(s) 692
VpaK11BI GGWCC 2 cut(s) 470, 674
XapI RAATTY 1 cut(s) 648
ZrmI AGTACT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.