AT5G26220

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010804
Physical Location & Seq
Reverse (-)
9162969 .. 9164884
1916 bp
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UTR
Exon/CDS
Intron
AT5G26220.1

Sequence Viewer

Length: 651 bp
ATGGTTTTGTGGGTATTTGGATATGGTTCTTTGATCTGGAACCCAGGTTTTGATTTTGATGAAAAACTCATTGGTTATATCAAGGACTATAAACGTGTCTTCGATCTAGCGTGTATTGATCATAGAGGTACTCCTGAACATCCTGCTAGGACTTGTACGCTGGAGCAATCCACCGGAGCTATTTGCTGGGGTGCTGCTTATTGTGTTCGTGGAGGACCAGAGAAAGAGAAACTAGCAATGGAGTACTTGGAACGAAGAGAGTGTGAATACGACTCAAAAACACTTGTTGAGTTTTACACTGAAAATGACACTTCCACGCCAATCGTTACCGGAGTTATTGTCTTCACGTCAACACCAGACAAAGTGTCGAACAAATACTACTTAGGTCCAGCTCCATTGGAAGAAATGGCGAGGCAAATCGCTACGGCTTCGGGACCTTGTGGGAACAACCGAGAGTATCTTTTCAAGCTAGAGAAAGCAATGTTCGACATTGAGCATGAGGAAGAGTATGTGATTGAGTTAGCGAATGAGGTTAGGAAGCAACTCGATTTACCGGAGGAAGTGAAGGCATTGTTGAAGCCTATTGTTTCTCATGTATCCGTCAAGTCTCAAGCTCATGTTTCCACTCGTCAACGGGTCTTTGCATCATGA

Protein Analysis

216

Amino Acids

24.57

Weight (kDa)

5.25

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 181 1.1e-46 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 3 cut(s) 130, 157, 245
AflIII ACRYGT 1 cut(s) 94
AgsI TTSAA 2 cut(s) 466, 577
AhdI GACNNNNNGTC 1 cut(s) 364
AjiI CACGTC 1 cut(s) 348
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AluBI AGCT 4 cut(s) 179, 392, 469, 614
AluI AGCT 4 cut(s) 179, 392, 469, 614
Alw26I GTCTC 1 cut(s) 612
ApeKI GCWGC 1 cut(s) 194
AspS9I GGNCC 3 cut(s) 215, 386, 434
AvaII GGWCC 3 cut(s) 215, 386, 434
BbsI GAAGAC 2 cut(s) 91, 334
BbvI GCAGC 1 cut(s) 181
BceAI ACGGC 1 cut(s) 441
BciT130I CCWGG 1 cut(s) 45
BciVI GTATCC 1 cut(s) 607
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 4 cut(s) 107, 147, 233, 470
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 3 cut(s) 215, 386, 434
BmeRI GACNNNNNGTC 1 cut(s) 364
BmgBI CACGTC 1 cut(s) 348
BmgT120I GGNCC 3 cut(s) 215, 386, 434
BmiI GGNNCC 2 cut(s) 41, 435
BmrFI CCNGG 1 cut(s) 45
BpiI GAAGAC 2 cut(s) 91, 334
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 594
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 3 cut(s) 173, 329, 553
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bse3DI GCAATG 2 cut(s) 243, 486
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 1 cut(s) 139
BseMI GCAATG 2 cut(s) 243, 486
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 186
BsiSI CCGG 3 cut(s) 174, 330, 554
BslFI GGGAC 1 cut(s) 447
BsmAI GTCTC 1 cut(s) 612
BsmFI GGGAC 1 cut(s) 447
Bsp143I GATC 3 cut(s) 33, 103, 118
BspHI TCATGA 1 cut(s) 647
BspLI GGNNCC 2 cut(s) 41, 435
BsrDI GCAATG 2 cut(s) 243, 486
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 3 cut(s) 33, 103, 118
Bst2UI CCWGG 1 cut(s) 45
Bst6I CTCTTC 2 cut(s) 250, 498
BstDEI CTNAG 1 cut(s) 382
BstF5I GGATG 1 cut(s) 139
BstKTI GATC 3 cut(s) 36, 106, 121
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 3 cut(s) 33, 103, 118
BstNI CCWGG 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 43
BstV1I GCAGC 1 cut(s) 181
BstV2I GAAGAC 2 cut(s) 91, 334
BsuI GTATCC 1 cut(s) 607
BtrI CACGTC 1 cut(s) 348
BtsCI GGATG 1 cut(s) 139
BtsIMutI CAGTG 1 cut(s) 297
CciI TCATGA 1 cut(s) 647
Cfr13I GGNCC 3 cut(s) 215, 386, 434
Csp6I GTAC 3 cut(s) 129, 156, 244
CviAII CATG 4 cut(s) 497, 593, 617, 648
CviJI RGCY 6 cut(s) 179, 392, 428, 469, 580, 614
CviKI_1 RGCY 6 cut(s) 179, 392, 428, 469, 580, 614
CviQI GTAC 3 cut(s) 129, 156, 244
DdeI CTNAG 1 cut(s) 382
DpnI GATC 3 cut(s) 35, 105, 120
DpnII GATC 3 cut(s) 33, 103, 118
DriI GACNNNNNGTC 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 250, 498
Eam1105I GACNNNNNGTC 1 cut(s) 364
EarI CTCTTC 2 cut(s) 250, 498
Eco47I GGWCC 3 cut(s) 215, 386, 434
EcoO109I RGGNCCY 1 cut(s) 434
EcoRII CCWGG 1 cut(s) 43
FaeI CATG 4 cut(s) 500, 596, 620, 651
FaiI YATR 9 cut(s) 24, 78, 90, 123, 498, 510, 594, 618, 649
FaqI GGGAC 1 cut(s) 447
FatI CATG 4 cut(s) 496, 592, 616, 647
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 1 cut(s) 195
FokI GGATG 1 cut(s) 126
Fsp4HI GCNGC 1 cut(s) 195
FspBI CTAG 4 cut(s) 107, 147, 233, 470
GluI GCNGC 1 cut(s) 195
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 182
HapII CCGG 3 cut(s) 174, 330, 554
Hin1II CATG 4 cut(s) 500, 596, 620, 651
HincII GTYRAC 2 cut(s) 351, 632
HindII GTYRAC 2 cut(s) 351, 632
HinfI GANTC 1 cut(s) 272
HpaII CCGG 3 cut(s) 174, 330, 554
Hpy166II GTNNAC 2 cut(s) 351, 632
Hpy188III TCNNGA 4 cut(s) 37, 134, 432, 648
Hpy8I GTNNAC 2 cut(s) 351, 632
HpyAV CCTTC 1 cut(s) 559
HpyCH4IV ACGT 2 cut(s) 94, 347
HpyCH4V TGCA 1 cut(s) 644
HpyF3I CTNAG 1 cut(s) 382
HpySE526I ACGT 2 cut(s) 94, 347
Hsp92II CATG 4 cut(s) 500, 596, 620, 651
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 3 cut(s) 33, 103, 118
LmnI GCTCC 3 cut(s) 163, 176, 397
Lsp1109I GCAGC 1 cut(s) 181
MaeI CTAG 4 cut(s) 107, 147, 233, 470
MaeII ACGT 2 cut(s) 94, 347
MaeIII GTNAC 1 cut(s) 325
MalI GATC 3 cut(s) 35, 105, 120
MboI GATC 3 cut(s) 33, 103, 118
MboII GAAGA 5 cut(s) 91, 267, 334, 413, 515
MlyI GAGTC 1 cut(s) 266
MnlI CCTC 6 cut(s) 119, 206, 405, 493, 523, 550
MspI CCGG 3 cut(s) 174, 330, 554
MspR9I CCNGG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 45
NdeII GATC 3 cut(s) 33, 103, 118
NlaIII CATG 4 cut(s) 500, 596, 620, 651
NlaIV GGNNCC 2 cut(s) 41, 435
PagI TCATGA 1 cut(s) 647
PkrI GCNGC 1 cut(s) 196
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
PpuMI RGGWCCY 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 434
Psp6I CCWGG 1 cut(s) 43
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 41, 435
PspPI GGNCC 3 cut(s) 215, 386, 434
PspPPI RGGWCCY 1 cut(s) 434
PsrI GAACNNNNNNTAC 2 cut(s) 362, 394
RsaI GTAC 3 cut(s) 130, 157, 245
RsaNI GTAC 3 cut(s) 129, 156, 244
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 3 cut(s) 33, 103, 118
Sau96I GGNCC 3 cut(s) 215, 386, 434
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 45
SinI GGWCC 3 cut(s) 215, 386, 434
SmlI CTYRAG 1 cut(s) 609
SmoI CTYRAG 1 cut(s) 609
SspMI CTAG 4 cut(s) 107, 147, 233, 470
StyD4I CCNGG 1 cut(s) 43
TaiI ACGT 2 cut(s) 97, 350
TaqI TCGA 4 cut(s) 102, 368, 486, 546
TatI WGTACW 1 cut(s) 243
TscAI CASTG 1 cut(s) 304
TseI GCWGC 1 cut(s) 194
TspDTI ATGAA 1 cut(s) 75
TspGWI ACGGA 1 cut(s) 589
TspRI CASTG 1 cut(s) 304
VpaK11BI GGWCC 3 cut(s) 215, 386, 434
XspI CTAG 4 cut(s) 107, 147, 233, 470
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.