Rroxscaffold_7G00162860

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010804
Physical Location & Seq
Reverse (-)
5225942 .. 5228384
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00162860.1

Sequence Viewer

Length: 675 bp
ATGGTATTCTGGGTGTTTGGATATGGTTCACTTGTGTGGAACCCTGGTTTTGAATACGACGAGAAAGTGATAGGTTTCATCAAGGACTACAGGCGTGTATTCGATCTTGCGTGCATTGATCATCGAGGTACGCCTGAGCACCCTGCAAGAACTTGCACATTGGAACACCAAGAAGGGGCTATTTGCTGGGGTGCTGCTTATTCTGTGCGTGGCGGCCCTGAAAGGGAAAGATTGGCTATGGAGTACTTGGAAAGGAGAGAATGTGAATATGATGCAAAGACTCTTGTGAACTTTTACAAGGAAGGGGACTTCTCTGATGAGCCTTCTTTGACAGGAGTCATTGTTTTCACATCTACTCCAGACAAAGAGTCAAACAAATATTACCTGGGTCCTGCTCCATTGGAGGATATGGCAAGCCAAATTGCGACTGCTTTTGGGCCATGCGGGAACAACAGAGACTACCTATTTTTGCTGGAAAAAGCTATGTTTGATATTGGCCATGAAGATGACTATGTGATAGAGCTTGCAAATGAGGTGAGGAATGTGCTTGGAGTTGTTGGGAAAGGAATTCCAAGGGATAAGAAGTTGAAAGGACCAACCCACATCCCGCTTAAGTCCCACATTCCAACCATTCAATTGCATCCCCTTCCGGAAGCCATCGCCATGGACTCGTAG

Protein Analysis

224

Amino Acids

25.32

Weight (kDa)

5.11

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 181 6.9e-45 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 649
AciI CCGC 3 cut(s) 213, 444, 608
AcoI YGGCCR 1 cut(s) 496
AcsI RAATTY 1 cut(s) 567
AfaI GTAC 2 cut(s) 130, 245
AfiI CCNNNNNNNGG 2 cut(s) 175, 223
AflII CTTAAG 1 cut(s) 611
AgsI TTSAA 3 cut(s) 53, 589, 635
AhdI GACNNNNNGTC 1 cut(s) 367
AjnI CCWGG 2 cut(s) 43, 384
AleI CACNNNNGTG 1 cut(s) 34
AluBI AGCT 2 cut(s) 482, 523
AluI AGCT 2 cut(s) 482, 523
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 450
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 3 cut(s) 214, 437, 496
ApeKI GCWGC 1 cut(s) 194
ApoI RAATTY 1 cut(s) 567
AspS9I GGNCC 4 cut(s) 215, 389, 437, 593
AsuHPI GGTGA 1 cut(s) 547
AvaII GGWCC 2 cut(s) 389, 593
BalI TGGCCA 1 cut(s) 498
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 665
BciT130I CCWGG 2 cut(s) 45, 386
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 450
BfmI CTRYAG 1 cut(s) 88
BfrI CTTAAG 1 cut(s) 611
BisI GCNGC 2 cut(s) 195, 214
BlsI GCNGC 2 cut(s) 196, 215
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 2 cut(s) 45, 386
Bme18I GGWCC 2 cut(s) 389, 593
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgT120I GGNCC 4 cut(s) 215, 389, 437, 593
BmiI GGNNCC 2 cut(s) 41, 390
BmrFI CCNGG 2 cut(s) 45, 386
BmsI GCATC 2 cut(s) 262, 649
BoxI GACNNNNGTC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 135
BsaJI CCNNGG 4 cut(s) 43, 385, 572, 663
BsaWI WCCGGW 1 cut(s) 649
BsaXI ACNNNNNCTCC 6 cut(s) 247, 277, 327, 340, 357, 370
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 223
BseAI TCCGGA 1 cut(s) 649
BseBI CCWGG 2 cut(s) 45, 386
BseDI CCNNGG 4 cut(s) 43, 385, 572, 663
BseGI GGATG 2 cut(s) 603, 640
BseLI CCNNNNNNNGG 2 cut(s) 175, 223
BseMII CTCAG 1 cut(s) 126
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 186
BshFI GGCC 3 cut(s) 216, 439, 498
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 650
BslFI GGGAC 2 cut(s) 320, 601
BslI CCNNNNNNNGG 2 cut(s) 175, 223
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 2 cut(s) 320, 601
BsnI GGCC 3 cut(s) 216, 439, 498
Bsp1286I GDGCHC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 649
Bsp143I GATC 2 cut(s) 103, 118
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 3 cut(s) 213, 444, 608
BspANI GGCC 3 cut(s) 216, 439, 498
BspCNI CTCAG 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 41, 390
BspTI CTTAAG 1 cut(s) 611
BssECI CCNNGG 4 cut(s) 43, 385, 572, 663
BssMI GATC 2 cut(s) 103, 118
BssT1I CCWWGG 2 cut(s) 572, 663
Bst2UI CCWGG 2 cut(s) 45, 386
BstAFI CTTAAG 1 cut(s) 611
BstC8I GCNNGC 3 cut(s) 112, 415, 525
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 663
BstF5I GGATG 2 cut(s) 603, 640
BstKTI GATC 2 cut(s) 106, 121
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 2 cut(s) 103, 118
BstNI CCWGG 2 cut(s) 45, 386
BstPAI GACNNNNGTC 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 43, 384
BstSFI CTRYAG 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 664
BsuRI GGCC 3 cut(s) 216, 439, 498
BtgI CCRYGG 1 cut(s) 663
BtgZI GCGATG 1 cut(s) 643
BtsCI GGATG 2 cut(s) 603, 640
Cac8I GCNNGC 3 cut(s) 112, 415, 525
Cfr13I GGNCC 4 cut(s) 215, 389, 437, 593
Csp6I GTAC 2 cut(s) 129, 244
CviAII CATG 3 cut(s) 441, 500, 664
CviQI GTAC 2 cut(s) 129, 244
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 105, 120
DpnII GATC 2 cut(s) 103, 118
DriI GACNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 496
Eam1105I GACNNNNNGTC 1 cut(s) 367
Eco130I CCWWGG 2 cut(s) 572, 663
Eco47I GGWCC 2 cut(s) 389, 593
EcoO109I RGGNCCY 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 567
EcoRII CCWGG 2 cut(s) 43, 384
EcoT14I CCWWGG 2 cut(s) 572, 663
ErhI CCWWGG 2 cut(s) 572, 663
FaeI CATG 3 cut(s) 444, 503, 667
FaiI YATR 9 cut(s) 24, 239, 270, 410, 442, 485, 501, 513, 665
FaqI GGGAC 2 cut(s) 320, 601
FatI CATG 3 cut(s) 440, 499, 663
FauI CCCGC 2 cut(s) 437, 615
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 2 cut(s) 195, 214
FokI GGATG 2 cut(s) 590, 627
Fsp4HI GCNGC 2 cut(s) 195, 214
GluI GCNGC 2 cut(s) 195, 214
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 3 cut(s) 216, 439, 498
HapII CCGG 1 cut(s) 650
Hin1II CATG 3 cut(s) 444, 503, 667
HinfI GANTC 4 cut(s) 280, 336, 368, 668
HpaII CCGG 1 cut(s) 650
HphI GGTGA 1 cut(s) 547
Hpy166II GTNNAC 2 cut(s) 29, 289
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 359, 650
Hpy8I GTNNAC 2 cut(s) 29, 289
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 4 cut(s) 167, 296, 333, 656
HpyCH4V TGCA 6 cut(s) 114, 146, 156, 275, 527, 640
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 3 cut(s) 444, 503, 667
Kpn2I TCCGGA 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 103, 118
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 2 cut(s) 262, 649
MalI GATC 2 cut(s) 105, 120
MboI GATC 2 cut(s) 103, 118
MboII GAAGA 1 cut(s) 515
MfeI CAATTG 1 cut(s) 635
MhlI GDGCHC 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 3 cut(s) 420, 567, 635
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 4 cut(s) 274, 345, 377, 662
MmeI TCCRAC 1 cut(s) 650
MnlI CCTC 4 cut(s) 119, 397, 526, 531
Mox20I TGGCCA 1 cut(s) 498
MroI TCCGGA 1 cut(s) 649
MscI TGGCCA 1 cut(s) 498
MseI TTAA 1 cut(s) 612
MslI CAYNNNNRTG 3 cut(s) 34, 504, 662
Msp20I TGGCCA 1 cut(s) 498
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 1 cut(s) 650
MspR9I CCNGG 2 cut(s) 45, 386
MunI CAATTG 1 cut(s) 635
MvaI CCWGG 2 cut(s) 45, 386
NcoI CCATGG 1 cut(s) 663
NdeII GATC 2 cut(s) 103, 118
NlaIII CATG 3 cut(s) 444, 503, 667
NlaIV GGNNCC 2 cut(s) 41, 390
OliI CACNNNNGTG 1 cut(s) 34
PkrI GCNGC 2 cut(s) 196, 215
PleI GAGTC 4 cut(s) 274, 344, 376, 662
PpsI GAGTC 4 cut(s) 274, 344, 376, 662
PpuMI RGGWCCY 1 cut(s) 389
PshAI GACNNNNGTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 43, 384
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 2 cut(s) 43, 384
PspN4I GGNNCC 2 cut(s) 41, 390
PspPI GGNCC 4 cut(s) 215, 389, 437, 593
PspPPI RGGWCCY 1 cut(s) 389
RsaI GTAC 2 cut(s) 130, 245
RsaNI GTAC 2 cut(s) 129, 244
RseI CAYNNNNRTG 3 cut(s) 34, 504, 662
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 2 cut(s) 195, 214
Sau3AI GATC 2 cut(s) 103, 118
Sau96I GGNCC 4 cut(s) 215, 389, 437, 593
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 4 cut(s) 274, 345, 377, 662
ScrFI CCNGG 2 cut(s) 45, 386
SduI GDGCHC 1 cut(s) 141
SetI ASST 7 cut(s) 76, 130, 387, 465, 484, 525, 537
SfaNI GCATC 2 cut(s) 262, 649
SfcI CTRYAG 1 cut(s) 88
SinI GGWCC 2 cut(s) 389, 593
SmiMI CAYNNNNRTG 3 cut(s) 34, 504, 662
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 3 cut(s) 420, 567, 635
SsiI CCGC 3 cut(s) 213, 444, 608
SspI AATATT 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 43, 384
StyI CCWWGG 2 cut(s) 572, 663
TaqI TCGA 2 cut(s) 102, 124
TasI AATT 3 cut(s) 420, 567, 635
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 216
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TseI GCWGC 1 cut(s) 194
TspDTI ATGAA 2 cut(s) 67, 516
Vha464I CTTAAG 1 cut(s) 611
VpaK11BI GGWCC 2 cut(s) 389, 593
XapI RAATTY 1 cut(s) 567
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.