Rw6G039360

Catalyzes the formation of 5-oxoproline from gamma- glutamyl dipeptides and plays a significant role in glutathione (GSH) homeostasis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010804
Physical Location & Seq
Forward (+)
63374960 .. 63377327
2368 bp
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UTR
Exon/CDS
Intron
Rw6G039360.1

Sequence Viewer

Length: 558 bp
ATGGTATTCTGGGTGTTTGGATATGGTTCACTTGTGTGGAACCCTGGTTTTGAATACGACGAGAAAGTGATAGGTTTCATTAAGGACTACAGGCGTGTATTCGATCTTGCTTGCATTGATCATCGAGGTACGCCTGAGCACCCTGCAAGAACTTGCACATTGGAACACCAAGAAGGGGCTGTTTGCTGGGGTGCTGCTTATTCTGTGTGTGGCGGCCCTGAAAGGGAAAGACTGGCTATGGAGTACTTGGAAAGGAGAGAATGTGAATATGATGCAAAGACTCTTGTGAACTTTTACAAGGAAGGGGACTTCTCTGATGAACCTTCTTTGACAGGAGTCATTGTTTTCACATCTACTCCAGACAAAGAATCAAACAAATATTACTTGGGTCCTGCTCCATTGGAGGATATGGCAAGCCAAATTGCGACTGCTTTTGGGCCATGCGGGAACAACAGAGATTACTTATTTTTGCTGGAAAAAGCTATGTTTGATATTGGTGAGCAATCTTTTTATGCGTCGACAGATGGTCAAAATTACACTGATTTGAGGGGGGTCTAA

Protein Analysis

185

Amino Acids

21.01

Weight (kDa)

4.59

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ChaC PF04752 3 - 165 3.5e-42 ChaC-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010804)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G31290 AT5G26220 AT5G26220
fragaria_vesca FvH4_2g36490 FvH4_3g36120
malus_domestica MD08G1124700.v1.1 MD15G1104100.v1.1
prunus_persica Prupe.1G456300_v2.0.a1
pyrus_communis pycom08g10340 pycom15g09450 pycom15g09490
rosa_chinensis RchiOBHm_Chr6g0305381
rosa_laevigata RLG00000010895
rosa_multiflora Rmu_co8139608.1_g000001 Rmu_sc0002782.1_g000008
rosa_roxburghii Rroxscaffold_7G00162860
rosa_rugosa Rorug06G0340000
rosa_samantha Rh6BG449800 Rh6CG465800
rosa_wichuraiana Rw6G039360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 518
AciI CCGC 2 cut(s) 213, 444
AfaI GTAC 2 cut(s) 130, 245
AfiI CCNNNNNNNGG 2 cut(s) 175, 223
AgsI TTSAA 1 cut(s) 53
AhdI GACNNNNNGTC 1 cut(s) 525
AjnI CCWGG 1 cut(s) 43
AleI CACNNNNGTG 1 cut(s) 34
AluBI AGCT 1 cut(s) 482
AluI AGCT 1 cut(s) 482
Alw21I GWGCWC 1 cut(s) 141
AoxI GGCC 2 cut(s) 214, 437
ApeKI GCWGC 1 cut(s) 194
AspS9I GGNCC 3 cut(s) 215, 389, 437
AsuHPI GGTGA 1 cut(s) 509
AvaII GGWCC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 181
BccI CCATC 1 cut(s) 518
BciT130I CCWGG 1 cut(s) 45
BclI TGATCA 1 cut(s) 118
BfmI CTRYAG 1 cut(s) 88
BisI GCNGC 2 cut(s) 195, 214
BlsI GCNGC 2 cut(s) 196, 215
BmcAI AGTACT 1 cut(s) 245
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 389
BmeRI GACNNNNNGTC 1 cut(s) 525
BmgT120I GGNCC 3 cut(s) 215, 389, 437
BmiI GGNNCC 2 cut(s) 41, 390
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 1 cut(s) 262
BoxI GACNNNNGTC 1 cut(s) 335
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 135
BsaJI CCNNGG 1 cut(s) 43
BsaXI ACNNNNNCTCC 6 cut(s) 247, 277, 327, 340, 357, 370
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 223
Bse1I ACTGG 1 cut(s) 237
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 43
BseLI CCNNNNNNNGG 2 cut(s) 175, 223
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 181
BseYI CCCAGC 1 cut(s) 186
BshFI GGCC 2 cut(s) 216, 439
BsiHKAI GWGCWC 1 cut(s) 141
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 175, 223
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 2 cut(s) 216, 439
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 2 cut(s) 103, 118
BspACI CCGC 2 cut(s) 213, 444
BspANI GGCC 2 cut(s) 216, 439
BspCNI CTCAG 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 41, 390
BsrI ACTGG 1 cut(s) 237
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 2 cut(s) 103, 118
Bst2UI CCWGG 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 112, 415
BstDEI CTNAG 1 cut(s) 135
BstKTI GATC 2 cut(s) 106, 121
BstMBI GATC 2 cut(s) 103, 118
BstNI CCWGG 1 cut(s) 45
BstPAI GACNNNNGTC 1 cut(s) 335
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 181
BsuRI GGCC 2 cut(s) 216, 439
BtsIMutI CAGTG 1 cut(s) 537
Cac8I GCNNGC 2 cut(s) 112, 415
Cfr13I GGNCC 3 cut(s) 215, 389, 437
CseI GACGC 1 cut(s) 504
Csp6I GTAC 2 cut(s) 129, 244
CviAII CATG 1 cut(s) 441
CviJI RGCY 6 cut(s) 179, 216, 236, 417, 439, 482
CviKI_1 RGCY 6 cut(s) 179, 216, 236, 417, 439, 482
CviQI GTAC 2 cut(s) 129, 244
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 105, 120
DpnII GATC 2 cut(s) 103, 118
DriI GACNNNNNGTC 1 cut(s) 525
Eam1105I GACNNNNNGTC 1 cut(s) 525
Eco47I GGWCC 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 43
FaeI CATG 1 cut(s) 444
FaiI YATR 7 cut(s) 24, 239, 270, 410, 442, 485, 513
FaqI GGGAC 1 cut(s) 320
FatI CATG 1 cut(s) 440
FauI CCCGC 1 cut(s) 437
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 518
Fnu4HI GCNGC 2 cut(s) 195, 214
Fsp4HI GCNGC 2 cut(s) 195, 214
GluI GCNGC 2 cut(s) 195, 214
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 342
HaeIII GGCC 2 cut(s) 216, 439
HgaI GACGC 1 cut(s) 504
Hin1II CATG 1 cut(s) 444
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HinfI GANTC 3 cut(s) 280, 336, 368
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 3 cut(s) 29, 289, 519
Hpy188I TCNGA 1 cut(s) 316
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 3 cut(s) 29, 289, 519
Hpy99I CGWCG 2 cut(s) 62, 520
HpyAV CCTTC 3 cut(s) 167, 296, 333
HpyCH4V TGCA 4 cut(s) 114, 146, 156, 275
HpyF3I CTNAG 1 cut(s) 135
Hsp92II CATG 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 103, 118
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 262
MalI GATC 2 cut(s) 105, 120
MboI GATC 2 cut(s) 103, 118
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 2 cut(s) 420, 532
MlyI GAGTC 2 cut(s) 274, 345
MnlI CCTC 3 cut(s) 119, 397, 540
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 1 cut(s) 34
MspR9I CCNGG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 45
NdeII GATC 2 cut(s) 103, 118
NlaIII CATG 1 cut(s) 444
NlaIV GGNNCC 2 cut(s) 41, 390
OliI CACNNNNGTG 1 cut(s) 34
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 2 cut(s) 196, 215
PleI GAGTC 2 cut(s) 274, 344
PpsI GAGTC 2 cut(s) 274, 344
PpuMI RGGWCCY 1 cut(s) 389
PshAI GACNNNNGTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 43
PspFI CCCAGC 1 cut(s) 186
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 41, 390
PspPI GGNCC 3 cut(s) 215, 389, 437
PspPPI RGGWCCY 1 cut(s) 389
RsaI GTAC 2 cut(s) 130, 245
RsaNI GTAC 2 cut(s) 129, 244
RseI CAYNNNNRTG 1 cut(s) 34
SalI GTCGAC 1 cut(s) 517
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 2 cut(s) 195, 214
Sau3AI GATC 2 cut(s) 103, 118
Sau96I GGNCC 3 cut(s) 215, 389, 437
ScaI AGTACT 1 cut(s) 245
SchI GAGTC 2 cut(s) 274, 345
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 1 cut(s) 141
SetI ASST 4 cut(s) 76, 130, 325, 484
SfaNI GCATC 1 cut(s) 262
SfcI CTRYAG 1 cut(s) 88
SinI GGWCC 1 cut(s) 389
SmiMI CAYNNNNRTG 1 cut(s) 34
Sse9I AATT 2 cut(s) 420, 532
SsiI CCGC 2 cut(s) 213, 444
SspI AATATT 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 43
TaqI TCGA 3 cut(s) 102, 124, 518
TasI AATT 2 cut(s) 420, 532
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 1 cut(s) 544
TseI GCWGC 1 cut(s) 194
TspDTI ATGAA 2 cut(s) 67, 333
TspRI CASTG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 518
ZrmI AGTACT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.